DIY Tissue Culture: How to Get Started for Less Than $200

[@juandiegosoledadbarajas]

I am a biotechnology engineer and it is wonderful to see this kind of micropropagation content! Let's hope that more people get their attention to this beautiful thing about science!

[@noobcup]

I had never heard of this until I came across this video. Now I’m freaking hooked

[@JoostinSteg]

Hello, fellow BioTech! Yes, I agree with you 100%, now I would add that a good source of information is a book titled, Plants From Test Tubes, which adds a little more science to the above video. PiJ, thank you for the amazing content.

[@rodvans6008]

30 years in Horticulturist here. We were never taught how to do tissue culture. I am excited to give this a try!

[@iamradrob]

I'm constantly astounded by how smart and resourceful young people are nowadays.

[@hillbillybackwoods]

Its the internet, it is God and Satan at the same time. Still better than a worthless degree though...LMAO.

[@Zayskibop]

Mycologist here, been dying to learn plant culture, this is such an incredible series (especially when you have everything you need) 🔥❤️😂

[@spackerinternational6131]

Hello fellow mycologist! At the start of the video she states that you can create millions and millions of plants this way. I know for a fact this is not possible in mycology as eventually genetic senescence creeps in after you've expanded mycelium X amount of times and you get diminished results and you have to start again from spores.. I'm curious if this is the case with plant cloning.

[@Zayskibop]

@@spackerinternational6131 what kind of fungi do you study as well?! 😌

[@spackerinternational6131]

@@Zayskibop mostly? The type that bruises blue.

[@rebeccaspratling2865]

​@@spackerinternational6131 same. 😂

[@sleepn_on_me2473]

@@spackerinternational6131it has to be. Thats why in general gene flow and gene mutation is good because you get an entirely new breed from an original population

[@onepiece666]

I have worked many years in many labs doing this for strawberry and other species. The fact contamination is not a major issue for you is a miracle since for many people, working with wild plants, contamination is always a problem. Great job

[@WhiteBeardHashtag1205]

its all about sterile technique. If guys can be really efficient with home mycology with home made contraptions, this shouldnt be an issue at all

[@haifutter4166]

Its all relative. I did mammal tissue culture. After that most other cell or tissue culture seems easy, even cell culture of Hela cells or other mammalian carcinogenic cell lines is much easier than working with wildtype cells.

[@DeezNuts-hf2xm]

@@haifutter4166 i remember doing a paper on Henrietta and her cells, still to this day i think about it randomly on just how invaluable her cells are to science and yet the horrible things she had to go through for her cells to end up that way as well.

[@ShineAqua]

I got an associates degree in horticulture, but couldn't justify travelling to the land grant school for a Bachelor's, so as a certified plant nerd, this is the raddest thing ever. I remember buying a pineapple plant propagated this way from Disney's The Land ride, it made me want to study plants and hydroponics, and I love combining tech and nature. Keep this up, please, and inspire the next generation of plant nerds.

[@literallyjustsomegirl]

It was actually great having your fiancee do it live, with your coaching and notes! I feel like you added a lot of really helpful details that someone with experience might otherwise forget.

[@jonathanbennett1957]

I even added gloves to my still air box when I was working with liquid culture making Petri dishes growing mushrooms

[@awnage]

26:06 "Plants are Science". This reminded me so much of one of my grad school classes from 20 years ago. Well done making it accessible to the world.

[@2010dragonclaw]

I'm sorry you got injured! I'm a surgeon and i remember the early days of putting blades on scalpel handles in class. Blades were flying everywhere!(especially with brand new handles, they were very difficult) 😂. Nowadays, nurses put them on for me in the OR or we use disposable scalpels in the clinic. Hope you are healing well, glad to see it didn't stop you for long. Keep up the good work! Thanks for sharing.

[@jenny-DD]

She's already got a man 😂

[@3231guppy]

Seeing you teach someone for their first time - that was actually EXTREMELY helpful and made me feel so much better and more comfortable about attempting this one day

[@esthershushanspikes4875]

I'm a general bio major and what you're doing is fascinating. I have adhd and not sure why but your delivery has clear direction and keeps my attention. :)

[@plantsinjars]

Thanks! I try to make videos that would have been helpful to me when I was first getting started.

[@PlantCellTechnology]

Thank you for helping make tissue culture accessible!

[@nee3029]

Your channel is the best on TC! 👍

[@PlantCellTechnology]

@@nee3029 Thanks for your support! And, you like our content.

[@FainTMako]

I've never seen a person express such love for the laminar flow hood and wanting to see your diy ones 🤩

[@cannabisscience]

Let's go! Great video series. In my plant pathology grad student years, I did tissue culturing to remove viruses from fruits and vegetables in a lab with probably $50k+ gear. Getting this down to $200 to bring the science to the masses is just awesome!

[@rosalinddenny1450]

Thanks!

[@plantsinjars]

Thank you!!!

[@ultrasoft5555]

As a biologist, I find your work very-very impressive, the content you share here is amazing, I am stunned! And of course I've just subscribed to your channel :)

[@IntenseVisuals]

This has to be the most interesting topic and video I've been recommended in a long time.

[@aaronwilliamsiscool]

I appreciate the vast knowledge you have on your passion, I hope your depth on this subject is appreciated & complimented daily. I can clearly see it takes many years & tons of knowledge to speak like how you’re speaking & I just hope it’s being appreciated in your work space… You are getting a lot of views so that’s awesome to see 👏 Even though I barely know about this subject, I can tell the complexity of the processes you had to study through trial & error & persistence just to upload this video Stay awesome!

[@ripoffrecords]

Nodes are generally the best plant material because they contain an accumulation of meristematic cells ~ the 'building blocks!'. I'm an arborist but I grow everything including mushroom and fern spore cultures. I just started tissue culturing birds nest fern prothalli because I need them to grow and multiple much faster. This was helpful thank you.

[@duleepgoonewardene]

Greetings from Srilanka. Very practical and informative. " The couple that works together , stays together."

[@TiberiusCowgill]

This popped up in my recommended feed, so glad it did. This content is truly amazing, and I'm shocked you haven't hit 100k let alone 10k subs yet. Keep up the great work, truly amazing and educational!

[@thehuntfortruth]

I looked into this years ago because I wanted to do this and it looks like now the technology finally exists!

[@plantsinjars]

The technology has existed since the mid-1900’s- it just hasn’t been very accessible! Hoping to change that

[@debbiefox6846]

Omgosh!! I am so excited your video showed up in my feed. I love plants and own about 140 houseplants. I love studying botany & science. You really broke this down into such an easy to follow tutorial. Loved this! I have always wanted to do this with orchids. I'd probably also do this with all my Orchids, Hoya, Dischidia, some ferns, Jungle cactus, Zig Zag Cactus and my philodendrons. Thank you for sharing and I am honestly so sorry you got injured badly. A 3 day hospital & severed tendon are a huge deal. I hope you don't have nerve damage & your rehab goes well. So thank you for your sacrifice for Science. I can't wait to binge your other videos abd my inner nerd is super stoked! Take care & get well soon.🫶💚🪴☘️🌵🌿🌱🍃

[@plantsinjars]

Thank you! I have a video on my channel about cloning orchids from stem propagations if that helps! It's an older vid so the quality isn't as good but the info still holds up. I am also an orchid lover :) Someone gave me a great idea this morning to make a video about growing orchid seeds in vitro.

[@ChipSchmidt-b8l]

Thanks, I have propagated plants for years. This is WAAAAY beyond anything I have ever considered! Ver exciting!!!!!

[@OrderUpASeven12]

I know y are too young to have seen a movie called "Green Fingers" But when I saw your arm and of course the subject matter of this video; I thought about that movie. Good stuff, I used to do a lot of micro for work and one particular company refused to buy a laminar flow because they6 said it was too expensive, so I was forced to do all the culturing in a small closed room just using basic aseptic techniques. Despite losing many cultures (fungi) to other non specific ones, the usual culprits, we did indeed succeed with "jam jars" and no "proper equipment" LOL.

[@Marinecreature]

please, please make a video of your aseptic way, for those of us who are going to have to try rough-and-ready versions of this!

[@brunoyudi9555]

found you because of youtube algorithm, i didnt even know tissue culture was a thing 20 minutes ago and here i am buying all this stuff off Amazon lol the algorithm knows me pretty well!

[@randimayes560]

Tell me how you did! Before I buy it all😊

[@Lemurai]

This is actually great, when I went through my biochem major, part of our senior project was industrial produce research & production then up scaling the process. We even did plasmid insertions for specific trait expressions. It was good stuff, we got to work with the Chemical Engineering class in teams to scale the process. I like things like this, it’s good to see younger people on KZbin doing productive things.

[@SansNeural]

One thing that struck me here is the quality of the comments... says a lot about the quality of her content!

[@tanveerkazi9352]

I am really sorry for your injury. You are a very wonderful person. God Bless🙏 You. 🌹🌹😘😘

[@michealkish2902]

After watching other tc channels, and reading plants from test tubes. I like yours the best. In my opinion they do things that seem odd in process. 🤷🏻

[@darreneast4443]

One of the best tutorials I've seen, regardless of subject. No relevant info left out, and only relevant info kept in. Both concise and complete. Thank you for taking the time to both create and share this, it's very much appreciated. Oh, and all the best for your approaching nuptials. May you both have a long and happy life together. Cheers!

[@semmett22]

I’m pleasantly Amazed!! 🤯 I am a rookie mycologist and had no idea this was a thing! She is Great, Funny and Realistic! FYI, people can get a still air box made from plastic on Amazon, they can sometimes be cheaper, also they do have disposable surgical blades that do not need to be changed. thank you for the introduction to this New hobby and look forward to all your videos!!🫶🏼

[@jeremykenward3082]

As a plant person (mostly edible/medicinal) that also dabbles in mycology, this video is great! So much cross over skills/equipment/techniques. Never even considered tissue culture at home as a thing...but I just may start exploring that this year, or over the winter. I already have a big chunk of the stuff. Thanks so much for sharing this!

[@working2bselfsufficient724]

I was thinking samething, same equipment used for growing mushrooms indoors.

[@d.jensen5153]

@@working2bselfsufficient724 Yup! Back in the day I bought a HEPA filter that worked _so_ well at creating a sterile field for nutrient agar inoculation w/o contamination. I'm sorry I threw it away! I didn't imagine I'd be doing green tissue propagation in 2023, or that it would even be useful for such a thing.

[@MB-zj3er]

Thank you for your video. I've been cloning female plants for years with rooting powder and rockwool cubes. I had never heard of tissue culture. So interesting the will to live that nature has put into plants.

[@ivanivanovich5904]

cannabis licensed professional here. what a great video!

[@bobnewkirk7003]

Fantastic Video, it was very informative and well put together. I'm a professional Microbiologist and can verify that your doing everything as well as you can given your limitations; still air boxes are great. I have long wanted to setup my own BSC but haven't dedicated the space or time to it yet. You may not want to introduce other contaminants into your space but this skill set translates almost 1-to-1 into other forms of Micro work. If you or Robert have any interest in home Fermentation or Mycology you could easily expand your setup to include them. Additionally, some of the ingredients you mentioned (like agar) may be available cheaper from a supplier outside of the dedicated tissue culture space.

[@theeflake]

You're my first Tissue Culture video but injuring yourself like that from your work has earned a sub. I look forward to learning more from your videos.

[@garden_creature]

Me with 9 dollars in my bank account: ah yes, this is the video for me

[@calculatedrush]

Please upload a video and teach us how we can reach the $9 milestone in our bank accounts

[@markmarsden9168]

I too would like to learn how to achieve such wealth ​@@calculatedrush

[@multiarray2320]

​@@calculatedrush its easy: you just need to find a 10 dollar bill and then cut off a corner to make it 9 dollar.

[@calculatedrush]

@@multiarray2320 I'm still having issues with step 1, finding the $10.

[@panpeter2969]

I am really impressed by the clarity of your video. And I look forward to see a follow up video how you separate those shoots and root them. Thank you!

[@squishy_madness]

thank you so much for this video! it was the best video i have seen so far on getting started in tissue culture. i appreciate you considering budget, depth of detail, showing the procedures, and for making it seem so approachable!

[@urbanfarmacademy]

Very happy I came to your page. I am a mycologist .. doing mushroom tissue culture for a while now and been really looking forward to plant tissue cultures and now I saw your page. Welldone and thank you for sharing

[@WhiteBeardHashtag1205]

Youre a Mycologist? What university did you earn your degree from?

[@jeremiahbullfrog9288]

This is amazing, I have been doing amateur mycology for some 2 years, had no idea you could do this with plants. Thank you for sharing your science.

[@sdqsdq6274]

? this has been done in the aquarium plants hobby for such a long time way before house plants

[@DarrinNeagoy]

This video just answered so many questions I had on tissue culture from just not looking into it. I always thought tissue culture was from scraping any area of a plant so you'd have a bundle of cells and from there a plant would grow in the medium. Makes sense that plants would be propagated from the node though. Maybe one day I'll be able to put this video to good use. Thank you!

[@jpdel4391]

I can't believe how much this taught me, great content and super informative! Thank you so much.

[@wildgophers91]

Man just stumbled on this channel. GREAT STUFF. Love the "show an amateur how to do it in real-time"

[@greensage395]

I am 60-year's old, and a Life-long plant Lover.....when I was around 13, I went into a Woolworth's that had a luncheon area/kitchen/diner. I found a Product Stand with Test-tubes containing clear gel and a small portion of plant growing in it. The Cardboard backing it was attached to advertised colorfully as "Grow anything in this medium", and I was captivated. To me it seemed like incredible Science to do such a thing, ...Cloning! I really wish I bought it, but knowing Regulations in those days, it was probably best I didn't considering the Ingredients you are talking about! :) I might of grown a few extra fingers, and then some! LOL

[@alejandratorres3857]

Amazing video ❤ I worked with tissue culture when I was in University at Mexico and we did not have enough money to buy proper equipment so we sterilized the material in a pressure cooker too and used baby food jars 😊 and they worked really well

[@tenekevi]

Really nice explanation. BTW, scalpel blades can be inserted manually, you just have to know the technique. During surgery, we place blades manually but remove used blades with a tool. Only to avoid slicing ourselves with a used blade.

[@Highlander.7]

Well put together video!! Very concise. Blessings and may you recover quickly

[@mikioashi3038]

Great video!! I started a tissue culture at home for 2-3 years. Here in Thailand, I'm trying to keep it low on the budget and spend less than 50$. I was scared of the surgery scalpel too. I using the X-acto knife, which uses the same style of blade but easier and safer to attach. Using a knife will make the wound on the ex-plant smooth and less susceptible to infection (from my experience). And I am afraid that the media will be contaminated so I heat them up in microwave and drop some bleach into each jar while still liquid and leave it till there is no smell of the bleach before using(for a couple of days), the result comes out great.

[@thecloneguyz]

I would be very careful with bleach since it's residual and it doesn't just wash away and it sticks around for years

[@evelynjunaviles2582]

loved the video !!! I think it was even better that Robert did it because he did everything we possibly would have done without knowing it was wrong. So it was like you were next to one of us, teaching us.

[@LaurenJacksonlocs29]

You can also use baking soda to up the PH and distilled white vinegar to lower it to save some extra bucks 😊 I love your videos! Thank you for sharing thorough easy to follow info on PTC✌🏽🌱✨

[@plantsinjars]

I love this tip! I wish I had mentioned it in the video

[@nolongerabandoned]

@@plantsinjars baking soda adds alkalinity but not pH. You could use a diluted NaOH (lye) or liquid calcium hydroxide. You did an excellent job on this video! I am looking at growing giant bamboo here in the Philippines commercially, and your video is the best explainer I have seen yet. Thank you!

[@TheDuckofDoom.]

Vinegar is a very poor choice it has chemical similarity to the auxins. Normally phosphoric, sulfuric, or nitric acid would be good choices in hydroponics as they are all plant nutrients, citric acid may also be good and does not effect the nutrient balance(food grade is often found with canning supplies at low cost). Phos is the most common hydroponic pH down. Non-adultrerated ammonia is a fair choice for pH up (it will convert to more stable ammonium as it grabs protons) but KOH or potassium carbonate are also good (not bicarb. aka hydrogen carbonate). Most other bases are either poorly soluble or will interfere with the biochemistry; sodium is generally tolerable in very small amounts though a few species are quite sodium tolerant. Nitric acid is a tricky one for normal hydroponics with large active plant growth, plants love to absorb both -NO3 anions and +NH4 cations in much larger quantities than other nutriants. The plants exchange -OH ions for nutrient anios which raises the pH and with nitric you can end up chasing the pH if not aware of the bigger picture. Likewise ammonium cations +NH4 get exchanged for +protons and this lowers the pH. So the two must be balanced, and that balance varies with plant specie. The situation is slightly different with tissue culture because of limited nutrient uptake, and the media is a gel so the bulk solution cannot readilly buffer local changes in pH.

[@LaurenJacksonlocs29]

@@TheDuckofDoom. wow, lots of info! But the whole reason I mentioned vinegar was the very affordable cost lmao because that was the point of the video? Yes, if you have a bigger budget and access, of course get the best of the best. All I know is vinegar is like 3 bucks for a gallon 😂 and my cultures have turned out great if I even had to use vinegar. As you know, before adjusting PH, it tends to be in the low 4 range. Vinegar isn’t needed unless someone went too far over, which is less likely. Btw, back on topic of budget, how much do the options you mentioned cost?

[@LaurenJacksonlocs29]

@@nolongerabandoned it literally changes the PH. Have you tried it? The meter don’t lie 😂 But baking soda is a great option for someone starting out on a budget. And if you do need to use it, you use a pinch of it. It doesn’t take much at all to raise the PH.

[@hummingbird3771]

You are my newest favorite smart person!! Thank you for sharing this knowledge and compiling the list of resources. I hope you are healing well, and I look forward to joining your youtube community. Please don't beat yourself up emotionally about the accident. We all have lapses in judgement.

[@TheSkyHive]

I found you today and I am happier for it. I have been growing mushrooms for 25 years and have always wanted to tissue culture. I have lab grade equipment thanks to my fungi obsession so I will only need to buy the media. I am ordering supplies from your links and getting started. Thanks for prodding me into action...PLUR

[@thecloneguyz]

We use UVC bulbs to STERILIZE our growrooms 5min intervals x 3 times per day LITERALLY KILLS ANYTHING ON CONTACT - Spores, bacteria, Insects. We use it as a PREVENTION TECHNIQUE NO CHEMICALS Make sure you're not in the room when the lights come on because it's highly cancerous to human skin but the plants love it and you can accidentally kill your mushrooms or your good bacteria with it if you're not careful

[@izaiahyopek5268]

My mom has a monstera that has some albino like patterns on the leaves that I’d love to tissue culture.

[@loicpage5640]

Congrats on the views for this video! Your passion and hard work is very inspiring . Keep up the great content.

[@ohoiboi9818]

Lol i didn't even realize right away as you were talking with your hands that you had a rap. Really glad you made this video. The fact that you include relivent useful information is so nice thanks. Hope your arm heals up ok

[@bkm8707]

And for your next video, can I suggest teaching us knife throwing? I kid, I kid! Excellent video, I really enjoyed watching it and learning about home tissue culture. The last segment was quite entertaining watching you try to teach us about aseptic technique while Robert fumbles around with his hands in a clear box, lost, but trying his best like any good man in a mall shopping experience. Kudos to you, Robert, for being supportive and a great sport about the task at hand!

[@sidecardude]

Great content!!!! Cannabis will be my go to for tissue culture, looks similar to propagating mushrooms in agar.....

[@larkbox8427]

Hi, Great vid. Your clean box is very similar to the one i use for my brewing yeast culture play.. However i use a pane of glass on the top of my box instead of inverting it onto its lid, which provides much better vision, and also helps stop inadvertently breathing into the box when peering in through the arm holes tho that could be just me..

[@skeletonboxers7336]

omg plants and tissue culture was THE hyperfixation i was doing before the tech layoffs started rolling through last year, sold off some clones and been job hunting since, but ur channel made me really happy to see this type of hobby again, honestly super excited to get back into it again after seeing this video. keep it up!

[@linzyweber7247]

Thanks for this! I have been reading a lot of books about getting started, and the visual representation is really helpful. Sorry to hear about your injury. Best of luck on healing.

[@plantsinjars]

Thank you! I’m glad you found it helpful

[@dylancarter4928]

this was a hidden gem for me. dont regret clicking on this video

[@noahplants1206]

I love it gorgeous and smart. I have been trying to get into this for years. After watching the video, I will make the attempt. 😅

[@ian11hendri03]

Fantastic and very impressive Ms Laur. Thank you so much for the great video. lots of work and time needed to do editing like that etc. Hope your finger has recovered. God bless you.

[@timturk1899]

Great video! I've cloned plants, and always thought Tissue Culture was only possible through highly trained scientists in a sealed lab..?! You sure busted that myth, lol! IDT anyone could have broken it down into a more simple, understandable method. A new channel to subscribe to! Many thanks, and much respect!👍💯✌️🙏

[@plantsinjars]

Thank you!

[@oldkingcrow777]

This is fantastic. I was literally commenting on people spreading misinformation (or just asking about) on succulent propagation from "cuttings" and i said it wouldnt work by cutting the leaf, except in a laboratory setting, talking about tissue culturing. 😂 I never considered this was something that could be done without lab equipment so i never even looked into it. Super cool, itll probably be a year or 2 before i ever try this, but ive built a couple flow hoods for mushrooms, so when i move into the new house eventually i think a flow hood will be one of my first projects. This will be second ❤❤❤

[@muddypawswanderlust]

I'm so happy you finally made this video! It helped me a lot! 🤓🙏

[@plantsinjars]

Thank you! I'm glad it was helpful!

[@lilolmecj]

I don’t expect I will actually do this, but I have been quite curious. Thank you for the concise but wide ranging tutorial! My son cut the tendon in his left thumb cutting a zip tie off a coil of rope, while camping a two hour drive from home, and just about as far from a hospital. It all worked out, he had an excellent surgeon, and four years later it is just a memory.

[@chrisb.5570]

What a great basics video! So sorry to hear about your finger. I'd like to try some chimera African violets since they can't be grown from leaves. I'm also interested in meristem culture to clear violets of INSV but that's a bit advanced for now.

[@plantsinjars]

Thank you! Clearing INSV through culturing meristem tc sounds extremely interested. Keep us updated on your progress!

[@FelicitousCitizenStruggler]

Thank you sister for your sacrifice in the service of our education, and for sharing.

[@hotdubtime]

People say without sterile air pressurization there's a risk of jar contamination. And that it's problematic to sterilize the medium without autoclave. Can we see the results?

[@DustyQatun]

There is definitely an increased risk of contamination, but with proper sterile technique, we can lower out error margin to the point that it doesn't matter to non commercial people. It doesn't really matter to us if we lose 2-3out of ten jars (Not even that much once you get your technique practiced) but for someone making 1 million jars it really adds up

[@neila.4289]

Pressure cooker can sub for autoclave…

[@WhiteBeardHashtag1205]

You can fit at the very least 7qt jars upright into a 23qt Presto pressure canner. Works just as well an an autoclave.

[@mountainmanorganicz7742]

If you couldnt tell by your views on this vid... TC is HUGE in the cannabis culture and industry. Not a whole lot of folks offering this info for free or willing to take the time to show. But anyways... Im no pro but i knew more than the basics on TC... I REALLY appreciate the subject, content and your channel. You now have a new loyal follower

[@1DwtEaUn]

As far as first plants to tissue culture strawberries because they are easier-ish and there are a few rarer varieties that I'd like to incorporate in breeding Premier aka Howard 17, and Catskill. Next would be peach, to preserve rarer types like Triogem.

[@plantsinjars]

I’ve been wanting to try strawberries! Let us know how it goes

[@hansoncrack]

I was into Mycology for nearly a decade. This is very similar in so many ways and will be easy to cross over. Thanks for making it easy to understand

[@NagashiChidorii]

Wow smart and GORGEOUS TOO! I love how thorough and attentive you are in all aspects of doing tissue culture at home. I've already been doing it at home so its nice to see what I have figured out already and compare it to your suggestions. Amazing. and thanks for the tendon sacrifice for this video lol

[@NagashiChidorii]

Holy crap just finished the full video and you even give ChatGPT tips (which was a great idea!!) + have good edits like showing yourself crying and trolling yourself lol

[@GeneralNOH]

tip on light meter; most phones have one for auto brightness. If you have a grow light with PPFD maps you can calibrate them and they are ususlly a good reference. As far as ph goes, the strips/ph indicators tend to be more accurate than the cheap meters. There are possibly reasons not to do this in these circumstances but a flame can be useful in sterilizing tools too, just becareful near alcohol obviously. Speaking of, a spray bottle of isopropyl is good for sterilizing equipment. Well presented video, makes it seem less intimidating overall👌

[@jeffravage3829]

Presterilized scalpel blades come in a foil pouch with a cardboard insert to allow attachment to the handle without removing the blade. Peel back the foil so the slotted shank is exposed. Grasp the pouch, with your gloved fingers tightly on the flat. This will allow you to insert the handle while the blade is secured, covered and still sterile. Dont ever take the blade out of the pouch without the handle attached. That way lies madness(or great ouchieness). Excellent video, easy for newbies to follow. Keep it up!

[@pr27038]

Thanks for this awesome work you've put together. Sorry you got injured. For anyone who will be using scalpels, I highly recommend using a needle driver to place and remove the scalpel from the handle.

[@marcfruchtman9473]

A really great resource. I do however urge caution with regards to any 'math' done by LLM AI such as chatGPT... it is very prone to error, so just be careful, and for important calculations, double check the calculations (at least in 2023)... BTW, awesome info about the recycling symbol. Just discovered this channel [subscribed]!

[@gurkengamingpvz21]

this will be needed by me in the next few years. thanks a lot

[@blakeamiller]

Do you have any recommendations for how to work with plant hormones that come in powder form? These are the only kinds I can find that are readily available in the UK!

[@plantsinjars]

Sure! For BAP, Kinetin, and 2iP, you would weigh 25 mg of the powdered cytokinin, add 3 or 4 drops of distilled water, then add 1 M HCL solution a few drops at a time until the cytokinin is dissolved. Then add 250 mL of distilled water and transfer to a sealed container for future use. The process for making auxin stock solutions (NAA, IBA, and IAA) is the same, but you would substitute either 1 M NaOH or KOH instead of using 1 M HCL. Wear proper PPE and work in a well ventilated area with the powders :) They are banned in certain countries for a reason

[@blakeamiller]

@@plantsinjars thanks so much!

[@filisildaanino5725]

Are you a Horticulturist???

[@plantsinjars]

@@filisildaanino5725 No I'm just a lady who really likes plants :)

[@user255]

@@plantsinjars The PGRs are banned for large scale (agricultural) usage. Not for lab usage. Most of them are harmless unless very high prolonged exposure. You are more likely to mess up yourself with NaOH/KOH or HCl.

[@ArmageddonAfterparty]

It's a joy watching the Frankenstein stuff you do. I will never ever think of reproducing any of it, but I find it endlessly interesting. Thanks.

[@BuilderBreakerRemaker]

Not nearly confident enough to take this on yet, but subbed from two accounts because you sacrificed your tendon.

[@plantsinjars]

You are more ready than you think! Let us know how it goes if you try it

[@autisticguitar666]

Just a point on PGRs. I used to work for a big horticultural company with a tissue culture lab. While we did use PGRs in some of the media (e.g. for meristematic cultures), the standart media usually was MS or 1/2MS (sometimes with slight modifications) and a higher sugar content you are using. These standart media were simply used for propagating and maintaining, and often did not contain any PGRs at all. This is because: 1: When transplanted back into soil plants can still be affected from the PGRs that were present in the TC Media. Since their environment changed this can lead to changed growth patterns. (Not good if you want to see actual differences of varieties) 2: Shipping. Since some of the produced plants would cross many borders, they sometimes needed to be PGR-free. Some countries don't allow you to ship in these compounds. In some countries it can be hard to buy PGRs as a hobbyist, but there are many things like coconut water or the bark of the willow (I think) that naturally contain these hormones and can be used as a substitute. Recipes with culture media with natural PGRs are available online. If it is just for propagation of already established tissue cultures, try a medium without hormones.

[@imikla]

Awesome video! You are really getting better at editing and creating with every video! Since I've done tissue culture many moons ago in a lab, things appear to have gotten a bit easier (we actually had to make our own MS Medium from scratch with the individual chemicals!). Now I'm working to get back into it in my home 'lab'. I have quite a few of the supplies, but I'm still not satisfied with any of the culture containers I can find. I do however... *really* like (dare I say ❤) those jars with the PP lids you are using. Can you please share where to get those?

[@plantsinjars]

Sure! They are the 240 mL culture vessels from Phytotech. Thanks for your comment! phytotechlab.com/culture-vessel-glass-70-mm-8-oz-240-ml.html

[@imikla]

@@plantsinjars Awesome! Thank you so much! And they have them with filter vent tops!

[@danieljheelan5256]

Hi Laur, Thanks a lot for sharing. I tried several times but each time the cuttings got contaminated. You don't get problem when you leave your tools in bleach? After my TC, I wash all tools with water and wipe them but all my tools get corroded each time I use and sterilize in bleach. Working with anthuriums these days. Daniel Jheelan from France

[@plantsinjars]

@@danieljheelan5256 You may want to try sterilizing everything (both the tools and your tissue culture media) in a pressure cooker or insapot before doing the transfers if you're having issues with contamination. I usually pressure cook everything at 15 PSI for 15-20 minutes. I have a few videos about this subject on my channel :)

[@ghettosteeve]

I’m sure it was somewhat frustrating directing a newbie to do the transfer process but I think I got a lot out of learning from the mistakes. Thanks for the video!

[@metatechhd]

🔬🌱👏 Your professional work in a research lab is truly impressive! It's amazing to see your dedication and the results you've achieved. Keep up the great job and continue pushing the boundaries of your field. 🌟💪 Your passion and expertise are inspiring, and I look forward to seeing more of your groundbreaking work. 🚀🔬 Keep the innovation flowing!

[@modernalien6949]

I cannnot wait to try this. I got a Blue Basil Culture from Disney and I’ve cloned many plants but wanted to get into tissue culture. I couldn’t find any videos about this at all a year ago. I’m so happy now 😂

[@joegurt4782]

:O ROBS IN THE VID!?!

[@lorlimann]

Ok wow, very comprehensive! Love the tutorial ❤ I hope you recover quickly from your injury 😢

[@Skirmitch]

Ok, I'll stop using my bare hands to set up my scalpels.....

[@jakersaridakis]

Well well well... Looks like I have a fun planty project to do over winter break 😁

[@zephyrwinkle6552]

Fun fact that symbol on your plastic is not a recycle symbol. It's a plastic classification symbol. This was a ploy by big plastic they made a symbol similar to the recycling symbol to trick ignorant customers into thinking that all plastic is recyclable. While more than just numbers 1 and 2 are recyclable, 1 and 2 are almost always the only ones recycled because the cost of recycling the other numbers is way more expensive.

[@Doktracy]

Thank you! I have a biochemistry background. Planning to do this with expensive aquarium plants.

[@joegurt4782]

UNDER 200 dollars!?!?!?

[@TechplantChannel]

That's a good price especially when you look at how expensive it can be without a great guide like this!

[@walterperrell4898]

Monsteras (decent ones) start @ $50

[@nikkiknight6936]

Can be cheaper if you already grow mushrooms

[@scinerd11]

​@@lazy5373 considering a mushroom is more closely related to a newborn baby than it is to a plant, i struggle to see the relevance of this comparison. Obviously the needs of all three organisms are radically different

[@audreyschilling9272]

He's trying so hard and its so hekkin cute!! The way you can see every single half error he did (note: yes it was his first time said half errors are borderline expected....) But he was like wait wait fix fix... So wholesome

[@shakysugars]

Plan to tissue culture the plants I like to keep. Cannabis, and thankyou for the video, the info on the media was essential.

[@joycelyn_walls]

I’m SUPER new and found out about tissue culture because I wanted to sell houseplants online. This video made it so much more approachable!!

[@chinupduck4849]

Still air box works for spore inoculation too, which i think is how the algos brought me here. Thank you, dear plant lady. So many possibilities...