How to Inoculate Grain Spawn Bags

[@MaggieF59]

Been watching your videos for a few months, and just bought one of your monotubs off of Amazon. Inoculating today. Thanks for the no-nonsense, simply explained videos.

[@cybersyber3602]

I hope u don't get Trich I got the ones off Amazon and somehow Trich got inside the grain bags idk how

[@toddsimmons6003]

It’s nice to hear someone who doesn’t sound like a burn out showing how this is done. Great video! 👍🏻😀

[@BoomerShroomer]

Thank you! :-)

[@Shitstain951]

Well unfortunately the “burnouts” created this industry for you, hippies and such trash amirite?

[@spewdog]

Well…depends on what mushroom you’re growin

[@giovanniabraham7653]

Who knows what videos you were watching 😂

[@vogelvogeltje]

How understanding and wise of you, the mushroom has taught you well 😂😂🤣

[@eeelblunto7377]

THANK GOD I FOUND YOUR CHANNEL! I ate some shrooms recently and I loved the experience so much that I had to give growing them a try. I am an amateur and for only having taking shrooms once I am both eager and motivated to harvest some of these beautiful things! Thank you for the detailed video and clear instructions on how to get going! Much love.

[@BoomerShroomer]

So nice of you! Thanks! Reach out to me in Instagram if you have more detailed questions and send pics!

[@ricky-sanchez]

What was the dosage of the shrooms you took?

[@eeelblunto7377]

@@ricky-sanchez I ate .9 grams, not too sure what strain but it was quite a ride :)

[@BoomerShroomer]

@@ricky-sanchez can you reach out to me on Instagram?

[@piltbike5289]

@@BoomerShroomer does the midwest grow kit bags come WITH spores? i can purchase everything i need to grow? had a life changing experience on mushrooms. i don’t trust to buy from others always from a close friend who has now moved away and would like to start a grow. i Emailed you as well thanks.

[@Piesoplenty]

Hands down the best, most thorough step by step instructional video I've found in the mushroom community. I have subscribed.

[@BoomerShroomer]

Thank you! ❤️🍄❤️

[@BryanHocking]

I'd use a jet flame lighter or alcohol lamp that burns clean for the needle.

[@IsedaAE]

This was exactly what I needed to see before starting my kit! Thx a million!

[@Actaeon2nd]

I second that observation!!! I'm old so I'll watch it a couple of times.

[@greymort8783]

I just started my first bag. I bought your tub kit and am very excited to see how they turn out.

[@lawanajohson1436]

How did your do? I am starting mine this weekend. Please keep your fingers crossed for me !

[@danielalt7508]

When it comes to desinfection alcohol, make sure to read the manual on how much of it must be applied. In hospital we must soak everything so wet it takes about 5 minutes for it to dry, or else there is a chance bacteria has not died.

[@jenmdawg]

Fantastic! I am so glad I found your channel. I finally feel confident enough to innoculate my first grain bag and start my first grow after watching this video.

[@BoomerShroomer]

Wonderful! Let me know if you have any questions. Thanks!

[@cg0401]

My 1st Video I think I’ve watched of yours. Thank you so much for the great narration. I feel like I’m in A classroom again😅 only this time I’m awake and paying attention.

[@JubeProductions]

Your videos gave me the motivation to start a bulk grow using a grain spawn bag and monotub. I just got my grain spawn bag and I will be inoculating it and I wanted to watch this one more time.

[@isaacgarcia2979]

Isn’t spawn already inoculated? Spawn already has mycelium growing

[@Torturantula]

​@@isaacgarcia2979 I'm willing to bet they have a sterilized prepackaged bag of grain and are planning to inoculate it, thereby creating a spawn. Not sure why the specific diction matters.

[@mattthomas1430]

Needles are gamma sterilized, so rubbing alcohol and flame cannot improve sterilization. Those steps can be omitted. When injecting, rather than pulling the needle out and re-inserting 3-4 times at different angles, just insert the needle one time straight in, inject a tiny bit, then manipulate the needle from side to side without pulling out of the injection port. Pulling out and re-inserting risks contamination. Good video. How was your flush?

[@BoomerShroomer]

Cool. Thanks! What about needles that you have already used?

[@youshowmeyours]

@@BoomerShroomer throw away used needles

[@kelseyevans8451]

I know this is old but, maybe you can still help? I’ve been told to flame the needles because the heat will cause the injection port to heal back better? Is this bs?

[@mattthomas1430]

@@kelseyevans8451 yeah, that’s bs. The injection port is self-healing. The flame does nothing to a sterile needle but leave carbon behind. I don’t know why people invent theories. Just ignorance I guess

[@tyed94]

That's all well and good for one bag. Replacing a needle for each and every jar or bag is obserd. Heat sterlization is effective and used when having to inject 100's of bags or jars.

[@MrDannyd9]

I appreciate the nice video with narration. Although there are a couple of things that seem odd for someone that did sterile environment work in college. -The flame sterilization of the needle doesn’t make sense unless you are concerned that you grain bag is already contaminated: the port, grain, and needle are clean. I think this is more important if you are cutting agar as you don’t want to transfer between plates or stratification for contamination. SIDE QUEST: the soot is sterile. -Spraying the needle is odd, as using good sterile technique will be more effective (placement of items, moving left to right, not breathing on the specimen, etc.)

[@nosferatuzodd4956]

Just inoculated inoculated my first spawn bag. Blue oysters. Can’t wait!

[@stonkmark941]

How did it go?

[@dianebryant4684]

THIS is the most detailed video ever, THANK YOU! I have watched many and I am so glad I came across yours 😄

[@uncoolasmr4847]

Great vid! 1 suggestion, after flame sterilizing the needle, there's no need to whipe with alcohol. By doing so, you are basically downgrading the sterility, since you are going from sterilized to just sanitized.

[@BoomerShroomer]

Thank you!

[@Firehunter420]

What about the soot left over on the needle? If you don't wipe it with alcohol swab, then what about the black soot left on the needle after flaming it? Will that contaminate?

[@uncoolasmr4847]

@@Firehunter420 no, the soot is completely sterile as well :)

[@uncoolasmr4847]

@Howard Hopkins just remember that there may be soot in your grain spawn. I forgot one time and thought there was contamination, but it was just the soot lol

[@Firehunter420]

@@uncoolasmr4847 Awesome! Thank you! I'm about to inoculate some bags for the first time tonight. It will be my first grow. I just want to be sure I do everything correctly and have no contamination. I am super excited!Another question: After I heat the needle do I need to squirt out a little bit of this solution before I inject? Just worried about maybe contamination due to dead spores from the heat? Idk

[@stevelove16]

Thank you! I found your tutorial really instructive! Fingers crossed for me and my first attempt!

[@BoomerShroomer]

Awesome! Let me know if you have any questions.

[@irunfromfights]

How’d it go?

[@Sir.Mr.DudeMan]

Can you share any advice? What went well or what went wrong?

[@GloUpCosmo]

I'm waiting on my Grain Bag to come in the mail and I'm super pleased with this video

[@Lolwutdesu9000]

This video should be renamed to "how to pointlessly sterilise everything 200 times and waste your time"

[@andyballenger38]

Very informative guide. Really curious to see how they progress. Thanks for sharing!

[@BoomerShroomer]

Thank you!

[@pyrojojo952]

Thanks Boomer for all your good lessons,we appreciate it!

[@BoomerShroomer]

My pleasure!

[@unclesam5913]

thanks for the fast and detailed instructions ❤

[@FattyFelix]

I like your videos and really appreciate your content. I don't mean to be a dick but there's just 1 thing I'd like to point out, liquid won't pour out of a syringe if you hold it pointing down, it would only occur if there was an air port at the top of the syringe allowing a pressure differential. I only say this as it makes sense to me to hold it pointing down to limit potential contamination for amateurs like me that only use a still air box. Look forward to more of your shared experience and wish you well!

[@gatorone]

Love your page. Keep up the good work ..

[@GlennHill-ol8qv]

Thanks!

[@BoomerShroomer]

Thank you!! 🍄🍄

[@jonnyneoross369]

Thank u! Was wondering if I could inoculate grain with spores. I’m gonna give it a try. Usually I put the spores to Agar dish. Wow that clump of spores you got in that syringe. 😮 Love the videos an thanks for the info.

[@RayRaysGrowhouse]

Just had a big problem with these bags. Wish someone could come help me learn where I went wrong.but a little after doing everything you just did, some brown sugar type dust formed at the top not sure if it was the spores or the bag

[@MiaArt13]

Awesome videos I have seen by far. Very informative and you list everything in great detail. 💜

[@BoomerShroomer]

Thanks so much!

[@meowijuana2322]

Suggestions for maintaining the optimal inoculation temp? Do you use a small heater?

[@BoomerShroomer]

Mushrooms grow best in a cool environment - but not too cold! 70 degrees Fahrenheit (21.1° Celsius) is an optimal temperature for colonization and fruiting. I typically keep a heater set at 70°F in the room, and this seems to yield good results during both stages. If you can't use a space heater and have to use a heating mat, I recommend placing a towel between the heating mat and your tub. Make sure not to set the heating pad too high, 68-70 degrees fahrenheit would be as high as I would go. If the temperature of the room in which you're growing drops below 65°F, then your mushrooms will grow very slowly, or possibly even stall. Avoid high heat as well: the mycelium has a death temperature of 104°F (40°C). Keep in mind that the temperature inside your monotub will typically be a few degrees warmer than the room it's in.

[@gamingcorneruk]

I have a question for you... If i was to take some Mycelium from my ready fruiting Mexicanas, could i add this small piece of Mycelium to a fresh batch of coco, brown rice and vermiculite and innoculate another batch? Thanks!!

[@Luca_Munz]

How sensitive is the bag to it’s environment once it’s been inoculated? Is it best for example to be in a cardboard box sprayed clean

[@rexcracin8642]

How long does one of these inoculated spawn bags last if it is not put into substrate immediately? what temp would you store it in?

[@XenoTravis]

Can you reuse a syringe by using the dried spores and just adding them to the liquid again?

[@dewy330]

You are a great teacher. Thank you.

[@jdibo]

Where the most ideal places to keep spawn bag while waiting for the mycelium to grow? I have mine in a card board box w a temperature of 75-76. Is it ok if I have it in a open room? (Basement). Hardly any traffic down there. And when people say in a dark place does it literally have to be pitch dark or is it fine in my basement with lights turned off, and as I said before nobody comes down here

[@josephwoodruff4102]

Great job. Very informative. This helps a lot bc my last try got watery and didn't take off at all

[@BoomerShroomer]

The bag probably had wetspot.. not your fault.

[@josephwoodruff4102]

@@BoomerShroomer I have some questions, my rye and casing came in. Would like to ask you things on how to fruit quickly. How do I or we contact personally ? No public platform?

[@cyberbid]

So, if I have a 10ml syringe do I inject all 10ml?

[@UMK3spaulding]

Can I put it back in the box where it was shipped while is colonizing?

[@davids11131113]

When prepping the bag spread out the grains and mix in the bag they’re tightly packed and just came from sterilization, you need to introduce air into the bag first. Way to much liquid into the bag too just 2 cc’s per pound is plenty you can do 2-3 bags per 10cc syringe.

[@takogoglidze4028]

Hello, how can I replace isopropyl alcohol for sterilization? I can’t find it here locally, what else can I use for sterilization?

[@cristinacassidy604]

Can I inoculate all in one bags with liquid culture instead of spore solution? I can not find a straight forward answer anywhere! Help! And thank you

[@Offftopic718]

Thank you! Please can you tell us what ratio to use spores/grain?! 🙌

[@mal.von007]

where do i buy a syringe i have a seed bag but it didn’t come with a syringe

[@plamenconev3387]

hi, what substrate can i use after colonization? I was thinking of using coconut bar + Brown rice, would that be enough? thanks in advance

[@SifedoXX]

One question please. I'm burning my mind on how to.know the pressure inside the pressure cooker. If its less than 15 bar it would not have the same efficiency. I have just a regular kitchen 5L pressure cooker. So again, how can I make sure everything is OK? Thank you legend

[@BoomerShroomer]

I ended up buying a pressure that had a gauge. That was the only way for me to know exactly what the pressure is.

[@Shg_Tob]

Do I have to cover the injection port or do I leave it with the puncture mark?

[@CarlosSanchez-bm3mq]

I had inoculated 96 Grain spawn bags with syringes from premium spores with exactly 3cc in each spawn (3pound bags) that were bought from Mycohaus. I turned off the ac injected the bags in a clean closed off area/ environment in my spare bathroom everything wiped down with 70% isopropyl alcohol along with spare closet in there then sprayed air with Lysol made sure everything was wiped down as in video. Temp was 75-80 in around march here in California spawn bags were white doing well took about 2-3 weeks for them to colonize 50% then broke them mixed them around waited about a week or 2 and they took longer then expect to fully colonize they all got about 70-80% some even 90% while waiting for all to be colonized they started to get “built up orange murky “water at the bottom of bags mushy type brown spawn towards the end did a little research to find out it shouldn’t smell sweet any reason why that happened?

[@hmk4216]

Is the mycelium always supposed to look like that? Does it matter what strain? I’m trying PE and there is definitely white stuff growing throughout the bag but it doesn’t look anything like that.

[@leec2101]

My package didn’t come w any of that , was I supposed to purchase the spores separate ???

[@everythingtesla1635]

Where is best place to buy spores ? Im sorry im new.. I looked on the website and didnt see them. Or maybe im looking for wrong thing

[@WOJOWNIK77]

How many CCs of the spore do you inject?

[@markebner5506]

What company did you buy your spore syringe from??

[@StefCR]

Where do you get your syringe with spores?

[@Byrian420]

I forgot to sterilize tje needle but never touched it when it came out the package is my bag contaminated now? Damn it 😪

[@BoomerShroomer]

Only time will tell, it could go either way. If you have already injected your bag, I would just see what happens. Best of luck.

[@JA-lj9od]

Great video . Very easy to follow and detailed . The best I have found so far . Where can I get liquid spores sirynges that are trustworthy

[@EM-ig2fw]

How many pounds of bulk substrate do I use per 2.5 lb bag of colonized grain spawn

[@20031792]

Hi, so the air is not important in the the grain bag. I so some others allowing air to go inside the bag before sealing it using impulse sealer.

[@BoomerShroomer]

Yes, air flow is important. You do need some airflow for germination and colonization in the bag.

[@tarkdemir6573]

Can i use parafilm to seal to tape it

[@jeffdworkin1780]

What is that last step? "ready to move them to your "monotub"? Whats a monotub?

[@wilkinsolk]

Its been 20 days since inoculation ,very little mycelium, bag is sweating? 78° ?

[@jld-ni3vf]

Where do you buy your mushroom grow bags with the injection port?

[@Ufccasual]

Love your content ashely!

[@chrisbergman9585]

Where is a good place to buy spores? I purchased some golden teachers from quality spores and followed your directions and I haven’t seen anything

[@taylorchristianson340]

If you inject too much spore liquid into the bag it can lead to bacterial growth from the amount of moisture in the bag. You typically don’t want to inject more than one cc of liquid into each bag.

[@isaacgarcia2979]

How much spore should you use per amount of substrate? Like how much per pound of substrate?

[@Chesterdacheeta]

Fuck

[@Chesterdacheeta]

She spreading bad info smh

[@isaacmartin7869]

😊What’s the lowest temperature that the room can be for the magic to happen?

[@MrSmokeingood]

How long does it take until you fist see signs of mycelium growth

[@OlajideEbiekuraju]

What is the liquid medium in the syringe in which the spores are mixed before injecting into the substrate?

[@nathanthayer]

how many cc of spore would you normaly use

[@thelovelydrkeyx3250]

Hi there, thanks for the video. What grains are being used and what liquid is in the syringe with the spores?

[@TrentonTrent]

Will you always have black specs in the syringe of spores? I bought some online and it's just a somewhat yellow liquid.

[@thesupreme7815]

Black specks is spore syringe. You got liquid culture. Liquid cultures can innoculate faster than spores

[@jamesparker7499]

How do you feel about grow bags? I've been wanting to try blue meanie and no one ever has any. Lol

[@friklefrunk743]

Are you doing infront of laminar flow hood or not?

[@xblackflagriderx]

I have probably watched this and many of your other videos 50 times each just because I want to make sure I do this absolutely correct, in preparation I've been monitoring areas in my apartment for the last 2 weeks as far as temperature goes and because I live in an old building and not all the windows are up to date it can get pretty cold, luckily it never drops below 55 in my apartment because I live in San Diego. My question and main concern is is that going to ruin any progress if the temperature drops below 70° for a few hours each day? Anxiously awaiting your reply as I just inoculated tonight

[@xblackflagriderx]

@@jocko1283 so funny story, this comment was about my second attempt because my first attempt on January 11th didn't seem to work and I thought I might have overheated my grain bags trying to counter the less then ideal temps I was dealing with. However, I was getting ready to toss the first attempt 3 days ago when I noticed it had some growth going on so they might be saved lol. As for the second attempt it's only been 7 days and so far no signs of mycelium. Fingers crossed though 🙂 feel free to message me directly if you want any more updates

[@xblackflagriderx]

@@jocko1283 yeah I have seen videos where it takes a week to see growth all the way to 45-60 days until growth so just wait a week before you even look at it again otherwise you'll end up like me checking every 10 minutes like something is going to happen haha where did you get your spore syringe?

[@xblackflagriderx]

@@jocko1283 where online?

[@squints1015]

do I want to have my bags rolled up with no airflow going through the patch or with it constantly exchanging air?

[@BoomerShroomer]

You need air flow. Leave the filter up.

[@squints1015]

@@BoomerShroomer I inoculated my bag with 10mls of Jack Frost LC and mixed it all around after and realized I'm so stupid for that. Is it ruined?

[@friklefrunk743]

Could i keep my bags in a sterilised monotub in a sterilised cupboard in the dark?

[@friklefrunk743]

In my room?

[@martinaespanya]

Hi Boomer! Just a question, I used 5cc of liquid culture instead of 2-3. Will that be fine?

[@damianallred3306]

So I’m doing my own grow now and I have noticed a fair amount of condensation in the bag is that okay

[@kev-in-space]

Is it a problem to have used the whole 10 cc's?

[@billygavlas8861]

Where can I buy those bags??

[@armysnoopy1562]

Can you make a video how to use the inflatable tub step by step with your spawn bag. Thank you,

[@Deverydoo]

I had no idea you had to shake the syringe that much. I shook it a lot and saw things floating around. I injected it all into a single place on my grow bag but nothing is showing 3 weeks later.

[@johngalt5825]

Did it work?

[@Rogelio-k5k]

What do I do if my spawn bag is dry?

[@2gooddoc]

I bought syringe liquid cultures instead of spores and they have been in my drawer for 2 months now. Is there a chance that I will still be able to get anything out of it?

[@HalfFrown93]

I’ve never had mine sit in a drawer; I always place in fridge after receiving but if I were you, I’d make sure you put that on some agar or test grains (some people don’t do agar. There’s a video that shows you how to not use agar if you’re not advanced in it yet). I’m sure it’ll be fine but sometimes LC’s can get weaker over time.

[@erekbrown3674]

What do you do with the second syringe?

[@juliusravus6509]

Can you inject the bag again after harvest?

[@diegooland1261]

My biggest concern is ordering syringes on line. Not for the spores or possible contaminants but it seems like a way for others to figure out where I live and what I'm doing. Is this concern unfounded?

[@BoomerShroomer]

Definitely thought about it. But we’re all just doing a bunch of research.. right? 🧐

[@cw4608]

Buy them at the livestock supply store. Murdoch’s, Tractor Supply, or similar. Pay with cash, you’re already wearing a mask. ;)

[@silas2722]

When can we expect your OF page?

[@BoomerShroomer]

lol

[@chemcruise2625]

If you only use half the spawn solution from the syringe, can the rest be stored for later use?

[@JoeDiMartino-jn1tf]

how do I buy my spores and portobello spores onley

[@CaptT49]

How to inoculate a Boomrbag without a inoculation port. I ordered the manure loving spawn bags!

[@BoomerShroomer]

What strain are you growing? Can you reach out to me on Instagram? Thanks.

[@Uytrghh]

Hi! I did everything correctly besides the fact I may have injected too many spores. I have a 2lb bag and injected around 10ml. Have I ruined it? It is a spore syringe.

[@BoomerShroomer]

No, it should be fine. Mix it around in the bag and then let it sit to grow. Wait 10 days to see if you have any growth.

[@Uytrghh]

@@BoomerShroomer okay thanks!

[@graphicxnature3195]

How did this go? That is a shit ton of solution injected.

[@BoomerShroomer]

@@graphicxnature3195 It went well. Colonized the whole bag.

[@graphicxnature3195]

@@BoomerShroomer I was actually asking @charlieoldman since he put 10ml in a 2 pound bag. I know you know what you're doing Boomer 😊

[@eliottbrown6959]

Is 77-80 degrees really the ideal temperature? Dang I’m gonna need a space heater for this bad boy

[@jjmjesse22]

How much is a average injection....can you over for it... and can you put to many spores in the syringe

[@BoomerShroomer]

Normally 2-5ccs. I've put the whole syringe in and it was fine.

[@mastart4971]

Hi Boomer, how do you prepare the spores? In what liquid do you put it to then inject it?

[@loganlaub4712]

Hi guys, I just had some questions. When I inoculate the spawn bag, do i kind of massage the bag directly after injecting or should not worry about it? Thanks in advance yall.

[@HalfFrown93]

Don’t do that or don’t do that again. IME, growth took forever for me to see progress. It was probably growing within the middle. I also can’t tell if you’re using MSS but I was using LC. You do not have to listen to me but if it works for you, it works for you. Most people I’ve seen say it’s a bad idea because you really don’t want to mess with the grains while they try to settle. Instead. Try to inoculate in different points of the bag and make sure you’re really careful for this part, by injecting towards the screen of the bag so you can keep track of progress

[@arthurrobey4945]

How did you get the spawn into the syringe?

[@ambersouther4775]

Where do you get you spore syringes?

[@Asherons]

I keep my AC on 69. Where am I going to find a spot that 77-80 degrees? Any idea?

[@Animal-Reaction-Clips]

Hi I made a mistake and I didnt realise until I did it. I inoculated the bag and I started mixing the grow bag and after 2 minutes of mixing I realised what I did . So its been 6 days since and I see no life yet. Do you think I would still see mycelium even though I mixed at innoculation??? Thanks a lot. Subbed too

[@BoomerShroomer]

Yes, they should still germinate. If you don't see any growth in like a day I would give the back a light mix and see if you can get them going.

[@Animal-Reaction-Clips]

@@BoomerShroomer sure thanks a lot i was worried a lot. Subbed to you too . Love your content ❤

[@EDsavant]

After you mix the bag what does it look like when it’s ready to fruit

[@toshiroolangar3855]

How long does it take from spore to a fully colonized grain bag? I just started recently but from spore to grain in a mason jar, it has been 4 days since I started the inoculation but I cannot see any activities yet.