Polymerase Chain Reaction (PCR)

[@skittless3949]

HOW DOES THIS GUY KNOW EVERYTHING IN LIFE?!

[@skittless3949]

SUCH a job well done.

[@drebomuche]

lmao

[@ynotspank1]

🎯

[@hasnainbaqir1072]

😅 He Is Really a Great Teacher

[@rosalineareola6844]

he's a physician

[@marys.2607]

I've been using your lectures throughout my undergrad, and now I'm in 1st year of 'Biochemistry & Molecular Genetic' PhD program. Yet, I still find your lectures very helpful. Thank you so much! 🙏

[@joyjohn7447]

Herbs are the best, I got cured completely using DR RORPOPOR HERBAL on KZbin herbs supplements to defeat PCR • his medication is active.......

[@ikaties1]

You are so precise and concise. It is truly a pleasure to listen to you lecture.

[@AKLECTURES]

+ikaties1 thank you! appreciate that

[@jonahinthewhale]

One little mistake on 2:02 You say that primer is "a relatively short sequence of DNA" but primer is a sequence of RNA

[@mikhailrotov6536]

@@jonahinthewhale he's talking about DNA primers

[@jazminbravo-contreras3018]

Honestly, if it weren't for you I don't know how I would pass my classes. Thank you for your organized lectures. You are literally the best on youtube!

[@jessicabursler4856]

YOU ARE AMAZING. You are saving my life in biochemistry, physics, and molecular biology. No one has ever explained anything better than you. Thank you!

[@playbeats6726]

Picked this video because I just KNOW Andrey will deliver and I WILL understand by the end of the video. Huge props to this channel for being the best at explaining hard biology concepts!

[@alexandermel9987]

You truly are gifted sir. I’m currently a PGY 1 surgery candidate and I’m having to do an entry basic medical science exam. Straight to the point. Short & clear. Big 👍🏾 from 🇵🇬.

[@nnenauchendu8681]

OMG! I'm prepping for USMLE and your lectures are awesome. Keep it up!

[@dannyholley]

Andrey, you have a real gift for teaching. I was glad to donate to your site this evening, as AK Lectures is one of the absolute best sources for instructional science content on the web. Thanks for making us all MUCH more knowledgeable!

[@AKLECTURES]

+Daniel Holley Hey Daniel, thanks for your donation, I greatly appreciate that! Thanks for all the kind words, happy that my content is helping! Have a great one!

[@MarmsRegineShop8]

I agree with you. I looked at other videos but AK lectures are the best. #Cheers

[@winelandashipala8413]

3 years later,im here to appreciate this channel. AK lectures is really the best source for science. thank you so much.

[@samad934]

thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.

[@abdifatahfarahhamud6426]

I am also like that, because biochemistry Examination in 30 marks, I have given by AK lectures Thank you, for 1000000 million times.

[@Someb0dy1Day]

I never donate to anybody, but you are an absolute genius to explain such complicated concepts and make them so simple to understand. I always look for your videos. I don't have much money to give as a student but i will gladly give you $10. Hope it is worth your while.

[@temguegael]

I have no words. absolutely amazing. Thank you. God bless u.

[@goldayanosilversea5775]

Much love from Kenya 🇰🇪🇰🇪🇰🇪🇰🇪

[@NelleJohn01]

I'm so glad I came across this channel. You truly have a gift for teaching. I understand everything perfectly. Thank you

[@1MoreStep1]

One of the best teachers in KZbin.

[@lindajanney7088]

May DR RORPOPOR HERBAL on KZbin leave long to fulfilled GOD promises in this life, your herbal supplement to my PCR worked like a magic, thank you for giving me completely healing may your days be long sir, I love you ❣️❣️❣️

[@songthanh896]

Really helpful to help me understand more deeply this technique. I'm from Vietnam so English is not my native language and studying biotechnology in the USA was really challenging for me. However, thanks for your videos, it makes a lot of sense for me to be clear to study this major and I graduated in Dec 2020. I watch your lecture videos everyday and still continue to watch them. My next step is to study graduate school to achieve my dream to become a scientist in biotech. I also recommended your channel to my friends who study biotech, biochem, or bioscience in Vietnam my home country. Super helpful, thank you a lot Andrey!

[@arianneducharme7542]

Thank you so much!! You are the only one on KZbin to do that kind of awesome videos of avanced biology!! I speak french and i understand everything you said, thanks a lot!!

[@Nilojan657]

this was by far one of the most informative video I have watched about PCR-- Thank you so much!!!

[@adimchinakaonyejekwulum3293]

Your videos are so detailed. You are a good teacher. Your videos have been so helpful. Thank you so much

[@anandrahangdale5214]

YOUR LECTURES ARE AWESOME , ITS HELPING ME TO PREPARE FOR AIPMT ( All India pre medical test) VERY EFFECIENTLY !

[@adlizulkefle4915]

This is truly awesome. I watch this for my final exam.

[@saltysuga9986]

this is a great video. you're really saving my life the day before exams!

[@AG-qm7be]

Superb, precise, and comprehensive at the same time...one of the few best teachers I encountered. Thank you so much.

[@AKLECTURES]

+alexander debru thanks for the kind words! :) appreciate it

[@AG-qm7be]

It is my pleasure!

[@goldayanosilversea5775]

Thank you , you've always made my life in university easier

[@NguyenNhung-wj1pi]

I've watched many your videos for learning. Thank you so much. You are a great teacher.

[@andreyapatinha6727]

U just saved my grade with this video. I love it. So informative!!!!

[@ezzaddeenalshuaibi6679]

Truly love the way you teach. You're amazing…

[@shealasuarez8797]

.....i think I love you! seriously just understood 3 weeks of microbial genetics lab/lecture from the last few videos. God bless you

[@meinbherpieg4723]

Great explanation. Thank you so much. You clearly know your stuff and are able to communicate effectively.

[@lailatulferdaus5317]

Thank you so much for making videoes that are very much understable.

[@amandak.5355]

I am learning so much from you! Thanks!

[@ngweforkum9252]

Tnx Andrey for this it helped me out to understand PCR after hectic time struggling to retain

[@tybksn]

Thank you, you made this so much simpler than my lecturer

[@AKLECTURES]

kofi gansah you're welcome! :)

[@paulosfissiha]

The most brilliant lecturer wow please add more ?

[@pensivenincompoop2016]

You are a great lecturer.

[@godlovemukwen]

I LOVE YOUR VIDEOS SO MUCH, YOU ARE JUST A COMPLETE TEACHER

[@ademolaajibade587]

Thanks. Really broke it down into tiny understandable pieces.

[@ibrahimmostafa372]

I got the idea by simple explanation,good job.

[@readytolearn77]

Wonderful and well put revision. God bless you!

[@faqa2111]

you are really helpful and gifted, you made my studying experience easier, thank you so much.

[@kimonobe278]

@AK LECTURES can you please tell us, Just roughly,What is the quantity of dNTPS and Primers required per cycle? Because the no of strand keeps getting amplified after every cycle,so that means more dNTP's,polymerase and Primers required. I will be really grateful if you reply.

[@manelyoona]

u make me curious n excited to know more,,, THANK U 💔

[@nadam3720]

such a great teacher. I will donate to your website with pleasure.. thanks

[@BasicInstinctx3]

This was absolutely amazing!

[@LyataTECHvfx]

I enjoyed and understood the lecture thanks to God who has given to you such amazing teaching talent.

[@rose12382]

THnx alot for all those great lectures

[@th-pp8uf]

Thanks a lot ❤From India

[@feriellarbi6857]

finallyyyy im so happy i finally understood PCR thank you so much .....u do awesome videos ^^

[@goldayanosilversea5775]

Thanks

[@justtry3914]

اجعل لك وردا يوميا من الصلاه على الحبيب المصطفى صلى الله عليه وسلم تفوز في الدنيا والاخره.

[@mochramadhan]

THANK YOU SO MUCH, YOU MIGHT SAVE MY SEMESTER

[@akum2010]

awesome lectures. Thanks

[@iamnobodyy]

You are the best of the best ❤

[@رندمنتظريوسف]

I love him so much

[@Nooba2010]

you are such a gr8 lecturer ,, keep up the good work ☺

[@Marmalade1986]

how come you're so smart? you're intelligence is amazing! unbelievable!

[@AnkushSharma-zv5hv]

nicely explained

[@elogewakwanyembo5534]

Well explained

[@Moones-ig1ks]

Thank's it's a useful lecture.

[@iliasshak1699]

thank you for nice explaination

[@MEDforyou]

Why is it that high GC content is favored for primers? This was a Q on practice MCAT! Thank you so much for your videos by the way. I'm using them to study for my MCAT

[@sajjadsaleem3014]

I think it is because g-c bond is triple hydrogen bond making it more heat stable than a-t bond. And in pcr - high temperature exposure is present.... and that may be the reason high gc content used as primer .

[@raanmouri9279]

Excellent work👍👍👍👍👍👏👏👏👏😘👏👏👏👏

[@soheillaghadery3637]

thank you sooooooo much.

[@arezofarhangi6153]

Great teaching, thank you

[@sruthi.m19]

amazing! clearly understood..thank you!

[@stockstechnicalanalysisnep849]

At last you said that PCR uses bacterial cell. so, doesn't it takes place in a beaker or a petradisk. i mean where is the use of bacterial cell here?

[@sumitaganguly2081]

Fantastic video, so well explained as always! Thanks a ton:)

[@parfoisbaker]

You explain it really well!!! Thank you so much!! :D

[@kaunispaikka9029]

You´re great! i´d love to find contents of this sort of in portuguese. Luck me i understand english!

[@DrAbhinayAnand]

Thanks for this video

[@smdr5576]

what about the Buffer system to stabilize the pH?

[@shaziasyed451]

awesome videos. great energy!

[@sruthi.m19]

what is meant by correct pcr? this is in the second cycle of pcr.. long product?

[@nurlatifahmohdnor8939]

Page 215 Le soleil = sun Sur = on Sous = under Si = balaa or na_m (Ar)

[@hasnainbaqir1072]

Please Upload A Lecture on Types Of PcR

[@samaryasser504]

thanks alot U R saving my life

[@kayihuragael2794]

At 12:35, what about the primers? Wouldn't you need to add more for further cycles?

[@AppleUploader]

Why can’t my “professor” be this good?

[@ealafabbas9745]

thank you sooo much for all your videos please l want to understsnd relative and absolute quantitation,efficiency,melting curve analysis, multiplex please explan 😢

[@javitoecua]

thanks for the video. I can ask a question, why in a PCR it is highly recommended to perform a premix instead of preparing the reaction tubes independently?

[@yiyiliu3175]

I like your presentation style although I didn't understand what you are talking lol

[@nonprophet8829]

So how does this fit into Infectious Disease Detection?

[@ryanmurdoch9581]

It doesn’t… They are calling our own genetic material viruses and calling viruses our own genetic material.

[@taxibeforesunsetclips7629]

By use with a prognosis and other diagnosis techniques. The detail is in the patents, I have one example on my channel.

[@yosefsbehat3013]

thank you

[@Blumble-bee]

Thank you 🤗

[@adisutesfaye2086]

you are amazing

[@harshalkelkar7970]

One question , 72 degrees is the ideal temperature for the DNA polymerase so initially while increasing it to 94 degrees at 74 degrees do we observe change? like in the first step because the temperature will definitely be at 74 for a few seconds at least . Will the polymerase get acivated maybe?

[@zeinab3699]

you are best best. Sorry i don´t have better words to describe you with

[@nealpatel4287]

THANKS MAN YOU"RE GREAT

[@malazhalawa7159]

You're Awesome

[@CxC2007]

how, if you can separate the 2 parts of the helix, you can use either one of the other ? how you select them?

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My trading journey was a matriculation of highs and lows, literally just like the market. you up, you down. Now I'm constantly up.+

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Over the past 5 years, buying Bitcoin every week performed better than timing the market 86% of the time.

[@M86I2001]

THANK YOU

[@احمدوائل-ط3ي]

Like before watching 🥰

[@Mr_i_o]

What are your thoughts on DNA transduction as evidence by empirical results done by Luc Montagnier?

[@carterrussell9725]

Great video. Sound is too low.

[@tybksn]

After each cycle why do we not replenish the nucleotides inside the beaker to make more DNA?

[@AKLECTURES]

kofi gansah They are there in ample amounts to continue it for many cycles.

[@bhuvandheenathayalan7048]

thumbs up for the videos ! great work!

[@nathaniele1835]

if i become a doctor, my first contribution is to you. i love you

[@Alialdarweesh]

very nice thank you

[@reemnour9837]

MANY THANX