C elegans Chemotaxis Assay

[@77palombo]

Hello, how are you? Great video on chemotaxis with C. elegans! I would like to know if you have (or where you got it from) the complete protocol, as I would love to use it.

[@sbbiovideos]

Hi Kenshiro, Happy to share here's the protocol we use for this lab: docs.google.com/document/d/1axDuy41I3ZZdNVmM55q_DOoF6Yq7qquGZsq8u1f0j5s/edit?usp=sharing

[@77palombo]

@@sbbiovideos Thank you so much !!!

[@77palombo]

Hello, here I am again. In my synchronization, I can't get only adult individuals. Young individuals always appear as well. Does this also happen to you? Is there any way to remove fragments of worms after shaking with the bleaching solution?

[@bigq5472]

No need to care about that. After bleaching, only eggs left are live.

[@francinecoa3553]

Hi Gustavo, I have used a cell filter (blue) to remove the bodies that are left over after synchronization. Thus, the eggs will be in the filtrated solution.

[@joshgraytox]

@@francinecoa3553 can you provide a catalog number for the cell filter?