Very nice technology and very good explanation, bravo!
@greta984 жыл бұрын
This explanation was great! Thank you so much!
@mspacey4 жыл бұрын
great video! best explanation of digital PCR I've seen so far
@stillatechnologies86344 жыл бұрын
Thank you! If you are interested in learning more about Digital PCR please visit www.gene-pi.com/
@SevenPhotonsFilms3 жыл бұрын
What a great explanation, perfect for understanding this specially in Covid times.
@alicewu25753 жыл бұрын
very clear explanation!
@geneluffy2 жыл бұрын
Nice video! Does digital PCR also need to consider the effect of non-specific amplification caused by primer?
@DivyanshBhadiar Жыл бұрын
Very helpful and informative Thankyou
@ojasvinibali14576 ай бұрын
Excellent video!
@ANZESIBOIDDI-b2m25 күн бұрын
I appreciate
@ivanaugustodelorena68423 жыл бұрын
Thanks for the great explanation!
@loicsavidan8186 Жыл бұрын
Very nice video, i would be grateful if you could give me a reference of the Poisson law to convert the data into copies/µL/ Thank you very much !
@chrspa20903 жыл бұрын
Why do you use fluorescence markers for the wild type allel in conventional qPCR if you are only interested in the mutation allel?
@stillatechnologies86343 жыл бұрын
In qPCR, as for the digital PCR, the wild type strain is often considered a reference used to compared data relative to mutations. The dPCR is a method which allows the absolute quantification of a template (DNA or RNA), but it is also used to produce comparative (or relative) results, one example could be the necessity to compare the fraction of mutated sequences out of the wild type ones in a certain sample. At the following link www.gene-pi.com/tutorial/introduction-rare-mutation-detection-2-3/ you can find an example of an assay of this kind.
@soura4 жыл бұрын
Why don't we construct a primer binding with the SNP therefore only the Mutant DNA would get amplified and we can visualize it
@stillatechnologies86343 жыл бұрын
Of course ! This could be also one strategy. Depending on what are your expectaction you can design the assay you prefer. The naica® system offers the possibility to have multiple channels which could allow at the same time, and in the same experiment, the detection of different targets.
@wayne020584 жыл бұрын
So you separate the sample before testing .?
@stillatechnologies86343 жыл бұрын
Yes, the PCR mix is loaded in our chips chips and then is partitioned in thousands of small droplets in which the template sequences (DNA or RNA) will be randomly distributed. For more information about our workflow, please check on our website here: www.stillatechnologies.com/naica-system-workflow/