Can We Trust Western Blots?

[@jazzmessenger8]

This video is gold

[@sarahsewdass9292]

Fellow Trinbagonian!! Currently at PSU. Good to hear a trini accent! Lol..missing my doubles:(

[@Iautus]

Basically, he just threw 10 months of my research to bin.

[@shishi6119]

This lecture actually upsets me for I don't realize that much possible problems... But still thans for sharing these info

[@mbi226]

Changed my whole perspective on WB during my postdoc studies....

[@marioduarte4167]

Dear Dr Aldrin Gomez, I'm from Brazil. We have a big problem with the disease Glanders in Brazil. The serology confirmation is done with WB. Conflict in the results make us very concerned about the test. Lots of horses are been kill. How we can check if they are doing right? Mario Duarte

[@Smiles5s5s]

Wow. Such a detailed presentation! Do all these problems mean that an ELISA would be better for quantifying proteins?

[@phatkaveh60]

Duh....

[@taylorbrooks4696]

Any new developments using PVA blocking? I've heard blocking can be achieved in less than a minute, but I am skeptical to try it out.

[@patrickcrawley346]

+

[@_Chafia]

Thank you so much :)

[@jelenazurkovic6254]

Perfect!!!!!

[@thamizh2850]

What concentration of tween 20 with TBS you use to block and dilute antibodies?

[@thamizh2850]

I tried to block nc membrane using tbst alone. But got dark blot. Why didnt work for me. Please give me your suggestion.

[@luisp.9710]

Hello. Can anybody tell me what he says at 10.42. ¨if you leave it .....of time¨ and 17:15 to 17: 29. Thanks

[@pingerthestinger]

Luis P. 10:42: This is identical samples. This is stained for just 1 minute with 5% BSA. This is stained for 1 hour with 5% BSA. 17:15: Talking about the amount of Ponceau S removed by water

[@luisp.9710]

Thank you.:)