Cell scraping

[@mohamedsabeelilislam1823]

Nice explanation, Thank you

[@thebumblingbiochemist]

Thank you

[@OctavioCastillo-h4n]

Hi! Great video. Question, is there a way to scrape cells for passaging and not kill them with a cell scraper? Would you use a cell lifter instead?

[@thebumblingbiochemist]

You typically trypsinize them to dislodge. kzbin.info/www/bejne/p3Wvo4F6i5pqa5Y

[@Europe-nk8dz]

This entire method is performed inside laminar flow?

[@thebumblingbiochemist]

Depends on the context

[@mayo_sandwich8161]

How much force do you usually put on the scraper?

[@thebumblingbiochemist]

Not much - good luck!

[@jacobmiller272]

Nice Video!! Hello, I'm currently working on cardiomyocyte primary cell lysis. I failed the experiment twice and I don't know whether it has to do with cell loss during the process. I went back to my protocol and assume primary cell aren't easy to attach to the dish; as a result, they could float away after removing the cell medium with followed rinsing with PBS. I have been trying to find articles talking about cardiomyocyte cell lysis protocols and journal, but so far to no avail. I have done protein extractions from other immortal cell lines before and it went well. What are some tips do you have for my particular circumstance? Should I change my protocol completely, or I guess I shouldn't rinse the dish with PBS before adding lysis buffer? Any articles that could help me with understanding primary cell? Thanks.

[@thebumblingbiochemist]

Sorry but I haven't worked with primary cell lines. I would try looking at the plates under the microscope before and after rinsing to check for loss. Good luck!