Quick overview of mammalian cell culture and cell counting
Пікірлер: 82
@alexandracapodicasa13333 жыл бұрын
Really great for someone who already has cell culture experience and just needed a quick crash course!
@tareksyrian24233 жыл бұрын
I can't agree more ! Exactly!
@Ratseeker2 жыл бұрын
In other words, he is being waay to casual going about this to nitpick on. Haha But still a great video.
@carolinamendez2542 жыл бұрын
This is the best video I have ever seen. Many congratulations, for having put your best effort into making it happen.
@JoshIbbotson3 жыл бұрын
This is extremely useful, thanks Neil!
@mr.gmadscientist88593 жыл бұрын
Thank you so much this was really helpful. Going to crush my interview now!
@cutlerwhitely22692 жыл бұрын
This is awesome, thanks! One note for anyone watching: Make sure you don't lay the pipette tip down with fluid still in it like at <a href="#" class="seekto" data-time="665">11:05</a>
@NewsIdeasInnovation3 жыл бұрын
Excellent video, very clear and informative.
@petersamuelemeka8710 Жыл бұрын
Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross
@greencard1245 Жыл бұрын
I love your videos! thank you!
@estefanycamilabomfimdossan2308 Жыл бұрын
This is an amazing work! Thank you so much!!
@funny117446 ай бұрын
Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?
@gitanjalideokar8482 жыл бұрын
Excellent efforts, very nicely explained
@vocalistguy98508 ай бұрын
Absolutely great
@hebaomar30013 жыл бұрын
Great job 👏
@kimmie202210 ай бұрын
Very thorough love this
@clairevernyuy58272 жыл бұрын
Thank you very much. This is very explicit.
@MohammedAmeenah12 күн бұрын
This is the best video have seen. Thank you so much
@oussboudjelthia4869 Жыл бұрын
Thank you soo much!!!! That was brilliant
@deepalishenoy6705 Жыл бұрын
Very nicely explained sir. Thanks a lot..
@brunadepaula5640 Жыл бұрын
It was very helpful!! Thanks!
@b-harmony11 ай бұрын
Thank you this was very helpful!
@prashantkumarparmanu2 жыл бұрын
REALLY GREAT .
@amansamriyar24803 жыл бұрын
véry good explanation sir
@taylormarkel1094Ай бұрын
this is so useful thank you so much for making this!!!!!!!
@aishahashmi2549 Жыл бұрын
Very informative 👍🏻👍🏻👍🏻
@shahnawaza6710 Жыл бұрын
Thank you for this nice video
@user-lp5qe1tv7d4 ай бұрын
Very nicely explained sir thank you very much 👍
@sarahmajin53582 жыл бұрын
Thanks for this video👍
@user-uf1kq3mi3u28 күн бұрын
Great vedio for cell culture indeed😊
@saminathapa829 Жыл бұрын
Very good video , thanks
@arghavanjafarijozani25923 жыл бұрын
Thank you
@welelameka2 ай бұрын
Thank you!
@danieloulhint7914 Жыл бұрын
Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.
@funny117445 ай бұрын
What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?
@kosheekaАй бұрын
@@funny11744 Phenol red actually can change its color from basic to acidic and then back again! But under two conditions it will not be a suitable component: 1. Multiple cycles of going from acidic to basic can degrade the phenol red over time, making it less effective as an indicator. 2. If the media becomes very acidic (much lower pH), the phenol red might not change back completely even if the pH is adjusted upwards. This is because the cells themselves might be stressed or dying, causing a permanent shift in the media's pH.
@funny11744Ай бұрын
@@kosheeka thank you for information.
@marwatawfik3956 Жыл бұрын
thanks so much
@Gts2pro2 жыл бұрын
Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,
@kosheeka2 күн бұрын
It is always a good practice to keep following it.
@WhatsInAName08 ай бұрын
Very helpful 👍
@hassangheisari59253 жыл бұрын
Great video!
@JYOtiRaNJanMANgaRaj3 ай бұрын
Thank u so much 🙏😊🙏🙏
@MES-S Жыл бұрын
An informative video
@user-ck4hp2eo2p3 ай бұрын
Very good presentation
@healthybutfathf89433 жыл бұрын
When you filling the 96well plate are you doing reverse pappeting?
@user-el5ur1tq1d3 ай бұрын
extremely helpful
@biologylover15653 жыл бұрын
Nice sir it help us lot in COVID situation.
@funny117446 ай бұрын
very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,
@kosheekaАй бұрын
While it's possible to count cells without Trypan Blue, this method doesn't assess cell viability. Trypan Blue helps differentiate between live and dead cells, ensuring you're working with a healthy population.
@Cornbreadddd2 жыл бұрын
I serial dilution for that plating solution?
@BlueSkyFight Жыл бұрын
No trypandblue?😮
@maggieweber84195 ай бұрын
Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?
@kosheeka29 күн бұрын
As you pointed out pouring cells out of the flask is generally not recommended. Pipetting is the preferred method for handling cells, especially primary cell lines which are more delicate. Pipetting allows for precision and reduced contamination risk.
@stooteebaruah967827 күн бұрын
I had the same concern
@valev28392 жыл бұрын
Thank you for this video, however I can't find the tutorial for counting cells :(
@user-fg6ub4dd3t4 ай бұрын
wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.
@sadiahaquekhan60032 ай бұрын
Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(
@canceronthebookss497526 күн бұрын
You can autoclave the media if you think any component of the media is causing it
@emilytravis24172 жыл бұрын
How are the cells adhering to the flask enough so they're not dumped out with the fetal calf serum?
@valev28392 жыл бұрын
Because he is using anchorage-dependent cells, cells that only grow if they adhere to a surface. The cells are simply doing what they would do in vivo, inside the body. Cells that belong to tissues produce molecules that mediate both adhesion to the surface and cohesion with each other in order to actually form the tissue. On the other hand, red blood cells for example are usually in circulation in our blood so they wouldn't naturally adhere to a surface.
@AmMalak-hd9di2 ай бұрын
Why he put 1.2 instead of 120 ? Someone explain please
@user-kc2uk2lv2h2 ай бұрын
Does this really follow aseptic practice? I doubt that。
@ISTARI222 жыл бұрын
Can I ask if there are any cells in the dmem media that you throw out or all cells only adhere to the flask?
@valev28392 жыл бұрын
cells adhered to the flask
@kosheeka27 күн бұрын
Until we add trypsin in the culture the cells stick to the flask, but after the trypsinization step, your cells will be in the medium so be careful.
@vijayaravindsubramanian93543 жыл бұрын
Sir explain how insulin cells are produced and insulin injection are prepared
@demonwolf61953 ай бұрын
Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?
@kosheeka24 күн бұрын
Washing with PBS removes unwanted components from cell cultures (dead cells, debris) and prepares them for further steps (staining, assays) without harming the cells. It provides a clean, consistent environment with the right salt balance for healthy cells.
@matthewwagner476 ай бұрын
Is breathing on it adding potential contaminating?
@kosheeka20 күн бұрын
Yes, breathing over the cell culture can be a source of contamination. You can introduce bacteria or fungi from your mouth and nose. In a sterile lab, wearing a head cover and surgical mask is essential to minimize this risk. Additionally, working in a biosafety cabinet provides further protection by filtering the air and creating a sterile work zone.
@AmruMagdy2 ай бұрын
@minato2363Ай бұрын
I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....
@kosheeka4 күн бұрын
The best practice is to avoid any type of cross-contamination during the study. Cross-contamination can be expanded to include chemical contamination that may occur if autoclave is not properly carried out. This is a common practice in many laboratories.
@samratpaul253 жыл бұрын
Are any animals killed during the extraction of Trypsin from their pancreas ? Or can it be done without killing the animal ?
@louisjacobmoon2 жыл бұрын
Why?
@samratpaul252 жыл бұрын
@@louisjacobmoon the basic idea of lab meat is to avoid killing animals, so if you are killing an animal to get Trypsin or something out of them so that the cells can feed-on and multiply then it defeats the purpose.
@neilcross94172 жыл бұрын
Many now use recombinant Trypsin eg TrypLE www.thermofisher.com/uk/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html?s_kwcid=AL.3652.3..e..o..tryple&ef_id=869904f24fa9112537fe6585ae811767:G:s&s_kwcid=AL!3652!10!76691033548341!76691086026937&cid=bid_clb_cce_r01_co_cp0000_pjt0000_bid00000_0se_bng_bt_pur_con&msclkid=869904f24fa9112537fe6585ae811767 However the foetal calf serum is most certainly animal-derived... There are some new but very expensive non-animal serum replacements
@lisaoney19732 жыл бұрын
Finally found a cure for my son Sickle cell I’m so glad you came into my life. I pray for long life so you can save more souls,Thanks #druromi .