Mammalian cell culture

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Neil Cross

Neil Cross

Күн бұрын

Пікірлер: 89
@alexandracapodicasa1333
@alexandracapodicasa1333 3 жыл бұрын
Really great for someone who already has cell culture experience and just needed a quick crash course!
@tareksyrian2423
@tareksyrian2423 3 жыл бұрын
I can't agree more ! Exactly!
@Ratseeker
@Ratseeker 3 жыл бұрын
In other words, he is being waay to casual going about this to nitpick on. Haha But still a great video.
@cutlerwhitely2269
@cutlerwhitely2269 3 жыл бұрын
This is awesome, thanks! One note for anyone watching: Make sure you don't lay the pipette tip down with fluid still in it like at 11:05
@mr.gmadscientist8859
@mr.gmadscientist8859 3 жыл бұрын
Thank you so much this was really helpful. Going to crush my interview now!
@carolinamendez254
@carolinamendez254 3 жыл бұрын
This is the best video I have ever seen. Many congratulations, for having put your best effort into making it happen.
@JYOtiRaNJanMANgaRaj
@JYOtiRaNJanMANgaRaj 9 ай бұрын
Thank u so much 🙏😊🙏🙏
@joel2757
@joel2757 4 ай бұрын
Great tutorial; it helped me to remember some basic stuff!!
@petersamuelemeka8710
@petersamuelemeka8710 Жыл бұрын
Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross
@MohammedAmeenah
@MohammedAmeenah 6 ай бұрын
This is the best video have seen. Thank you so much
@albinaamangaliyeva756
@albinaamangaliyeva756 Ай бұрын
This is so helpful! Thank you so much!
@Zhi-u6j
@Zhi-u6j 10 ай бұрын
Very nicely explained sir thank you very much 👍
@JoshIbbotson
@JoshIbbotson 3 жыл бұрын
This is extremely useful, thanks Neil!
@NewsIdeasInnovation
@NewsIdeasInnovation 3 жыл бұрын
Excellent video, very clear and informative.
@alethea1158
@alethea1158 4 ай бұрын
This video was very helpful, thank you so much!
@SumiKhatun-y9i
@SumiKhatun-y9i 6 ай бұрын
Great vedio for cell culture indeed😊
@Gts2pro
@Gts2pro 2 жыл бұрын
Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,
@kosheeka
@kosheeka 6 ай бұрын
It is always a good practice to keep following it.
@home4429
@home4429 4 ай бұрын
Super informative, thank you so much!
@nikinajafi3823
@nikinajafi3823 3 ай бұрын
Really helpful thanks! I needed those tips
@psirer..9625
@psirer..9625 5 ай бұрын
Thank you for the content.
@danieloulhint7914
@danieloulhint7914 2 жыл бұрын
Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.
@funny11744
@funny11744 11 ай бұрын
What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?
@kosheeka
@kosheeka 7 ай бұрын
​@@funny11744 Phenol red actually can change its color from basic to acidic and then back again! But under two conditions it will not be a suitable component: 1. Multiple cycles of going from acidic to basic can degrade the phenol red over time, making it less effective as an indicator. 2. If the media becomes very acidic (much lower pH), the phenol red might not change back completely even if the pH is adjusted upwards. This is because the cells themselves might be stressed or dying, causing a permanent shift in the media's pH.
@funny11744
@funny11744 7 ай бұрын
@@kosheeka thank you for information.
@funny11744
@funny11744 Жыл бұрын
very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,
@kosheeka
@kosheeka 7 ай бұрын
While it's possible to count cells without Trypan Blue, this method doesn't assess cell viability. Trypan Blue helps differentiate between live and dead cells, ensuring you're working with a healthy population.
@aishahashmi2549
@aishahashmi2549 Жыл бұрын
Very informative 👍🏻👍🏻👍🏻
@taylormarkel1094
@taylormarkel1094 7 ай бұрын
this is so useful thank you so much for making this!!!!!!!
@JosephKyalo-q5t
@JosephKyalo-q5t 9 ай бұрын
Very good presentation
@kimmie2022
@kimmie2022 Жыл бұрын
Very thorough love this
@maggieweber8419
@maggieweber8419 11 ай бұрын
Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?
@kosheeka
@kosheeka 6 ай бұрын
As you pointed out pouring cells out of the flask is generally not recommended. Pipetting is the preferred method for handling cells, especially primary cell lines which are more delicate. Pipetting allows for precision and reduced contamination risk.
@stooteebaruah9678
@stooteebaruah9678 6 ай бұрын
I had the same concern
@greencard1245
@greencard1245 2 жыл бұрын
I love your videos! thank you!
@vocalistguy9850
@vocalistguy9850 Жыл бұрын
Absolutely great
@WhatsInAName0
@WhatsInAName0 Жыл бұрын
Very helpful 👍
@amlmohamedelshabasy
@amlmohamedelshabasy Ай бұрын
THANK YOU VERY MUCH I REALLY APPRECIATE THAT
@clairevernyuy5827
@clairevernyuy5827 2 жыл бұрын
Thank you very much. This is very explicit.
@estefanycamilabomfimdossan2308
@estefanycamilabomfimdossan2308 2 жыл бұрын
This is an amazing work! Thank you so much!!
@funny11744
@funny11744 Жыл бұрын
Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?
@oussboudjelthia4869
@oussboudjelthia4869 Жыл бұрын
Thank you soo much!!!! That was brilliant
@b-harmony
@b-harmony Жыл бұрын
Thank you this was very helpful!
@marwatawfik3956
@marwatawfik3956 Жыл бұрын
thanks so much
@deepalishenoy6705
@deepalishenoy6705 2 жыл бұрын
Very nicely explained sir. Thanks a lot..
@hebaomar3001
@hebaomar3001 3 жыл бұрын
Great job 👏
@BlueSkyFight
@BlueSkyFight Жыл бұрын
No trypandblue?😮
@amansamriyar2480
@amansamriyar2480 3 жыл бұрын
véry good explanation sir
@gitanjalideokar848
@gitanjalideokar848 3 жыл бұрын
Excellent efforts, very nicely explained
@brunadepaula5640
@brunadepaula5640 2 жыл бұрын
It was very helpful!! Thanks!
@sadiahaquekhan6003
@sadiahaquekhan6003 8 ай бұрын
Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(
@canceronthebookss4975
@canceronthebookss4975 6 ай бұрын
You can autoclave the media if you think any component of the media is causing it
@welelameka
@welelameka 8 ай бұрын
Thank you!
@shahnawaza6710
@shahnawaza6710 2 жыл бұрын
Thank you for this nice video
@saminathapa829
@saminathapa829 Жыл бұрын
Very good video , thanks
@MES-S
@MES-S 2 жыл бұрын
An informative video
@sarahmajin5358
@sarahmajin5358 2 жыл бұрын
Thanks for this video👍
@arghavanjafarijozani2592
@arghavanjafarijozani2592 3 жыл бұрын
Thank you
@healthybutfathf8943
@healthybutfathf8943 3 жыл бұрын
When you filling the 96well plate are you doing reverse pappeting?
@AdeelHassan-v1v
@AdeelHassan-v1v 9 ай бұрын
extremely helpful
@ZainAbbas-i3e
@ZainAbbas-i3e 10 ай бұрын
wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.
@prashantkumarparmanu
@prashantkumarparmanu 2 жыл бұрын
REALLY GREAT .
@刘涛-c6y
@刘涛-c6y 7 ай бұрын
Does this really follow aseptic practice? I doubt that。
@Cornbreadddd
@Cornbreadddd 3 жыл бұрын
I serial dilution for that plating solution?
@demonwolf6195
@demonwolf6195 9 ай бұрын
Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?
@kosheeka
@kosheeka 6 ай бұрын
Washing with PBS removes unwanted components from cell cultures (dead cells, debris) and prepares them for further steps (staining, assays) without harming the cells. It provides a clean, consistent environment with the right salt balance for healthy cells.
@hassangheisari5925
@hassangheisari5925 3 жыл бұрын
Great video!
@AmMalak-hd9di
@AmMalak-hd9di 8 ай бұрын
Why he put 1.2 instead of 120 ? Someone explain please
@biologylover1565
@biologylover1565 3 жыл бұрын
Nice sir it help us lot in COVID situation.
@emilytravis2417
@emilytravis2417 2 жыл бұрын
How are the cells adhering to the flask enough so they're not dumped out with the fetal calf serum?
@valev2839
@valev2839 2 жыл бұрын
Because he is using anchorage-dependent cells, cells that only grow if they adhere to a surface. The cells are simply doing what they would do in vivo, inside the body. Cells that belong to tissues produce molecules that mediate both adhesion to the surface and cohesion with each other in order to actually form the tissue. On the other hand, red blood cells for example are usually in circulation in our blood so they wouldn't naturally adhere to a surface.
@ISTARI22
@ISTARI22 2 жыл бұрын
Can I ask if there are any cells in the dmem media that you throw out or all cells only adhere to the flask?
@valev2839
@valev2839 2 жыл бұрын
cells adhered to the flask
@kosheeka
@kosheeka 6 ай бұрын
Until we add trypsin in the culture the cells stick to the flask, but after the trypsinization step, your cells will be in the medium so be careful.
@matthewwagner47
@matthewwagner47 Жыл бұрын
Is breathing on it adding potential contaminating?
@kosheeka
@kosheeka 6 ай бұрын
Yes, breathing over the cell culture can be a source of contamination. You can introduce bacteria or fungi from your mouth and nose. In a sterile lab, wearing a head cover and surgical mask is essential to minimize this risk. Additionally, working in a biosafety cabinet provides further protection by filtering the air and creating a sterile work zone.
@valev2839
@valev2839 2 жыл бұрын
Thank you for this video, however I can't find the tutorial for counting cells :(
@samratpaul25
@samratpaul25 3 жыл бұрын
Are any animals killed during the extraction of Trypsin from their pancreas ? Or can it be done without killing the animal ?
@louisjacobmoon
@louisjacobmoon 3 жыл бұрын
Why?
@samratpaul25
@samratpaul25 3 жыл бұрын
@@louisjacobmoon the basic idea of lab meat is to avoid killing animals, so if you are killing an animal to get Trypsin or something out of them so that the cells can feed-on and multiply then it defeats the purpose.
@neilcross9417
@neilcross9417 3 жыл бұрын
Many now use recombinant Trypsin eg TrypLE www.thermofisher.com/uk/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html?s_kwcid=AL.3652.3..e..o..tryple&ef_id=869904f24fa9112537fe6585ae811767:G:s&s_kwcid=AL!3652!10!76691033548341!76691086026937&cid=bid_clb_cce_r01_co_cp0000_pjt0000_bid00000_0se_bng_bt_pur_con&msclkid=869904f24fa9112537fe6585ae811767 However the foetal calf serum is most certainly animal-derived... There are some new but very expensive non-animal serum replacements
@vijayaravindsubramanian9354
@vijayaravindsubramanian9354 3 жыл бұрын
Sir explain how insulin cells are produced and insulin injection are prepared
@AmruMagdy
@AmruMagdy 8 ай бұрын
@minato2363
@minato2363 7 ай бұрын
I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....
@kosheeka
@kosheeka 6 ай бұрын
The best practice is to avoid any type of cross-contamination during the study. Cross-contamination can be expanded to include chemical contamination that may occur if autoclave is not properly carried out. This is a common practice in many laboratories.
@lisaoney1973
@lisaoney1973 2 жыл бұрын
Finally found a cure for my son Sickle cell I’m so glad you came into my life. I pray for long life so you can save more souls,Thanks #druromi .
@mrx4814
@mrx4814 Жыл бұрын
wow can you share your work or publications?
@lisaoney1973
@lisaoney1973 Жыл бұрын
@@mrx4814 Yes sure
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