This video shows many things you should avoid during cell culture
@JYOtiRaNJanMANgaRaj6 ай бұрын
Thank you so much
@agwholland9 жыл бұрын
Nicely made video, thanks! You might also consider Gibco's 'Tryple' which is said to be more gentle on cells. Also, I would view cells on the microscope prior to detaching adherent cells, to see if they were confluent or not. If not confluent, then no need to trypsinize yet. Well made video!
@dsdeshmukh40246 жыл бұрын
Is it right way to use nonsterile tissuepaper to sterilize equipment with IPA? & Can we keep trypsin under cell culture bottle up to finish of trypsinization in incubator,what are its drawbacks?
@Suebe196210 жыл бұрын
Why did they leave the lid off the flask for so long....that's just asking for contamination to go right in there.
@margaretsmoot406110 жыл бұрын
nice to see they use sterile pipettes instead of cannulas, not concerned about the lid of the culture being left off so long in a BSL-2 style hood (they are not bench tops where contamination is a higher risk) they just move hepa filtered air around (risk is low). However the risk to touch the inside of the flip top lid of the trypson holding cylinder & then contaminate the next users cell line is a HUGE concern. really need to think about using a screw cap for this part.
@samratpaul253 жыл бұрын
Are any animals killed during the extraction of Trypsin from their pancreas ? Or can it be done without killing the animal ?
@martinasandstrom31657 жыл бұрын
Make sure not to use wristbands as shown in this video that´s not proper sterile technique.
@MasterofVirology2 жыл бұрын
Use sleeved covers as well
@aymanedaiz67662 жыл бұрын
thank you so much from Morocco for the vidéo. I have just a question do you think 5 minutes it's too long, maybe 1-2 min sholud be enough.
@ariajohn372 жыл бұрын
Depends on the cell line.
@aymanedaiz67662 жыл бұрын
@@ariajohn37 Thanks Aria ✌
@trombonbe4228811 жыл бұрын
What cell line is being passaged? I ask because the five minute trypsinization is fairly long, so I wonder if the cells are a very adherent bunch.
@MasterofVirology2 жыл бұрын
You need to consider cell density and percentage of trypsin. For example A549 cells would talks longer than 293 HEK, but 293HEK at a high seeding density could take longer than 5 minutes
@ShadyContinues5 жыл бұрын
Left that T75 flask cap on the bench... suppose to hold it upside down Yikes !
@blackbean664 Жыл бұрын
The cells must not be left without medium or Trypsin solution for 5 mins
@davvader2 жыл бұрын
Nooo, don´t spray into the LAF, this hurts the filters.
@davvader2 жыл бұрын
And the caps of the flask with the open side up!
@xxkkb86852 жыл бұрын
look at that furry bracelet on the right wrist :)
@AzureRath976 жыл бұрын
pie pet
@Pacarana18 жыл бұрын
WAW!! If you perform cell culture for pharma purposes, don't look this video.Nevertheless, I "like" for the clownish effect... ;-)