Chapter 7.2: Making a Bio-Filler presented by Dr. Richard Miron

In this video, an explanation for how to make biological fillers (AKA Bio-Filler) using White/Blue Top Tubes is presented. Noteworthy, by remixing the buffy coat zone, the denatured albumin gel is re-infiltrated with many platelets, leukocytes and growth factors to further assist in the generation process, all while maintaining longer stability when compared to standard PRF. The Albumin gel + liquid PRF (Alb-PRF) mixture (often referred to as extended-PRF or e-PRF) has been shown to last 4-6 months instead of 2-3 weeks found in standard PRF.
Understanding Platelet-Rich Fibrin Full Textbook is available for purchase at www.prfedu.com...
For research articles on Alb-PRF, please read:
bio-prf.com/re...
For info regarding ordering tubes or the Bio-PRF system, please visit www.bio-prf.com
Full textbook and Hands-on courses are available at www.prfedu.com/
Please leave comments below.

[@geoffsummer5866]

So it’s an amazing filler , bio compatible , gel consistency , what would you do to break down or dissolve this filler if there was a problem , Vascular or arterial occlusion , or intravenous injection how could you break it down fast if needed in an emergancy

[@armenkarimyan7074]

Dr. Marion I was wondering why did you choose to centrifuge 700g for 8 min instead of 2000g for 8 min?

[@Lizeeeeee.e.e]

This is also something I wish he answered because now im wondering if I should be spinning all my injectables at 700g. Very confusing.

[@guntherludgar]

Any news on the exact difference? The KZbin video's, articles published and the book PRF in facial aesthetics don't give me a clear answer. Also where does the 300RCF/5minutes protocol comes in and when 2.000RCF/8 minutes vs 2.000RCF/5 minutes? In the book PRF in facial aesthetics they also talk about 2.200RCF/8 minutes for albumin gel. So really getting confused.

[@kellymcallister2024]

Thank you so much for these instructions. I don’t have a bio-cooler, can you please tell me what temperature the bio-cooler is set to and is it possible to achieve the same cooling results with a sterile bowl of cold water monitored by a thermostat?

[@Hillaryalsbury]

What is the maximum quantity recommended for one session for someone who is wanting a larger volume such as a body area?

[@ginarominej5808]

What should the ratio of albumin heated to liquid be, 2 to 1 for total of 3? Is it important to get the bubbles out before mixing? Thank you, its an excellent video.

[@onlylovel.o.v.e]

Dear Dr, how many RPM IS 700 RCF? PLEASE EDUCATE US THANKYOU I LOVE UR VIDEOS VERY CLEAR

[@onlylovel.o.v.e]

Dear Dr how cold is the bio cool?? Thankyou

[@brcmedhealth]

Thank you for your videos! My Bio-Cooler arrived yesterday and my heater. I would like to know where to order the blue caps or can I use a similar cap device ?

[@prfedu]

You can order those also from www.bio-prf.com!

[@gracech123]

Am I able to use regular syringes or do I need specific syringes?

[@86jinyoungan]

How long this Bio filler last o body? Can this replace normal HA filler.? Can I use bio filler for lip augmentation..?

[@ExposeClothing]

How long does biofiller last in the body? Also does the cooler make the albumin same as body temperature?

[@prfedu]

Typically 4-6 months. Very similar half-life to Botox

[@goharabrahamyan4358]

What happened to the Tubes which were first placed in the bio cool after drawing up the 2 cc of ppp. Because in the video , I noticed the PPP from the bio heat was mixed with the 2 tubes on the rack snd not in the bio cool

[@prfedu]

Those were pulled out of the Bio-Cool and those are the 2 tubes you see in the video which are then mixed with the heated albumin gel.

[@huongphan5059]

3' 52" ?

[@Learn2SingInEnglish]

Is it ok to transfuse the remaining of the blood back to the patients after separating prf, IF the tubes were kept in the biocool device after the spin?

[@chrisKyle7274]

I would definitely recommend that NOT be done.

[@heatherdiaz4380]

That is a big NO NO NO!

[@winnie6504]

can we use A prf as a biofiller instead

[@prfedu]

No, A-PRF will not act as a Bio-Filler. That is an older technology for the early 2010s but doesn't last long in the body (1-2 weeks).