In this video I am showing the click reaction which won the 2022 nobel prize in chemistry! Support my channel with patreon: / chemiolis Follow me on twitter: / chemiolis
Пікірлер: 214
@AkashS97 Жыл бұрын
I am a PhD student and doing Anti cancer drug discovery and targeted drug delivery. We frequently use click chemistry to couple linker-drug with antibody. I think Click chemistry has ease the process of drug discovery by more than 50%. Super easy and quick to make a triazole ring.
@inspiringmuslim Жыл бұрын
Wow...that's interesting. U r doing in India or abroad??
@AkashS97 Жыл бұрын
@@inspiringmuslim At an Israeli university
@inspiringmuslim Жыл бұрын
@@AkashS97 Wow..that's amazing. I m currently an 11th grader in India interested in cancer research I m a pcmb student preparing for neet...can u just guide me a bit u know like how it all goes...like I m solely interested in research and I m interested in applying to IISC or universities abroad...plz guide me a bit brother
@inspiringmuslim Жыл бұрын
@@AkashS97 Thank you so much for your valuable suggestion and yeah kvpy has been abolished from this year its through neet rank under 5000 one can get into IISC. Best of luck for ur future endeavors 👍🏻
@markhays170 Жыл бұрын
I was shadowing in a lab that does anti cancer drug design with click chemistry too recently! This lab was doing it by releasing carbon monoxide at target cells, do you also work with CO-releasing molecules/drugs or is it something else?
@kaezaklimber3391 Жыл бұрын
DUUUDE, replicating nobel chemistry is THE GOATEST idea. Beautiful !!!
@Jeremy.Bearemy Жыл бұрын
Goatest??? 🤮
@selfactualizer2099 Жыл бұрын
you do realize anyone can repeat scientific projects right? it was published my guy.
@stefangadshijew1682 Жыл бұрын
Click chemistry being convenient to replicate is kind of the point, isn't it? :D
@SodiumInteresting Жыл бұрын
@@Jeremy.Bearemy greatest of all timeest I guess 🤷
@dipankarbanerjee1130 Жыл бұрын
Nowadays, Dictionary is frequently getting new costumes
@That_Chemist Жыл бұрын
6:36 this is how you know you are a real chemist now - this is exactly the type of trick you would see from a clever peer in a research lab
@user-mp4bc9qp2x Жыл бұрын
triturating? we teach this method of purification to undergrads. you must be lucky if youre making molecules that are soluble in most things.
@Acute. Жыл бұрын
My two favorite KZbin chemists ❤
@stefangadshijew1682 Жыл бұрын
@@user-mp4bc9qp2x I think the point is that he made an observation and used it to choose a purification method that he knew would work and would be convenient. If I was his peer, I would beg him to shake his sep funnel more vigorously and use a slightly bigger stir bar, but hey, if it works, it works. :D
@HaIsKuL Жыл бұрын
Which part? Removing the impurity with 5% ethyl acetate and hexate or the vacuum filtration?
@mnxs8 ай бұрын
@@HaIsKuLthe former
@patricksweetman3285 Жыл бұрын
Click chemistry can run in living cells. It well deserves it's Nobel Prize.
@myronwilde491 Жыл бұрын
As a practicing chemist I have 2 tips that will save you much pain and time. 1) Brine washes. Any time you do an organic/aqueous extraction and your product is in the organic layer, wash it with brine before any drying step. I've made very polar substances that will go into water, but they don't go into brine. So it doesn't hurt yield (unless you spill) and it seriously cuts down the amount of drying reagent and time required to achieve a dry organic layer. 2) Buy an aquarium air pump. Spending a day doing column chromatography for such simple reactions is just pain. You can speed most columns up (not advisable with DCM/MeOH, but great for all other solvent systems) by doing it under pressure. Even a cheap fish pump is designed to maintain a steady, sometimes variable, low pressure without over-pressurizing. It will accelerate chromatography substantially for not many dollars.
@brannigandupreez2083 Жыл бұрын
Flash column chromatography is the way to go, man. Ever heard of countercurrent chromatography?
@bromisovalum8417 Жыл бұрын
Definitely, a final brine wash also pre-dries your organic layer to a certain degree. Also helps if emulsion formation at the separation layer is an issue. Regarding flash chromatography, I've seen it done in those big kitchen syringes.
@humr2346 Жыл бұрын
one advise for column chromatography: Use sorption of crude product on small amount (5x mass of crude product) on silica gel. Disolve the product, add silica and evaporate. Than just pour it on the top of silica slope in column. This way you avoid diffusion at the begining of chromatography and problems like you had.
@brannigandupreez2083 Жыл бұрын
This seems a good point.
@jakob_212 Жыл бұрын
Celite as a carrier also works very well, gets nice and dry so is easy to get into the coloum
@andrewg.carvill4596 Жыл бұрын
5:30 I was a working chemist 30 years ago, before changing career completely (Catholic parish priest!). Happening on your expertly made video brought back to me the fascination of working the "classical" methods of synthesis and purification, and the sense of achievement experienced in upping yields. I'm wondering whether the low solubility of the product could be used to advantage in looking for recrystallization procedures from solvent or solvent mixtures in which the product is much less soluble than the impurities. Might this be worth a try (in place of the column) at this stage ?
@habboUdviseren Жыл бұрын
Watch the discussion between Mohammed Hijab and Jordan Peterson, then the discussion with Jordan and Hamza Yusuf
@stefangadshijew1682 Жыл бұрын
I think it definitely should be possible to find a good crystallization strategy, the products and educts are different enough. It actually pains me a bit to see chromatography being a used in a process that is advertised as being easy and convenient. No criticism to the maker of the video intended, just the principal of the thing. I'm working in organic process development, using chromatography automatically kills a process in our price range. I'd rather use harsh conditions and poor yield, but be able to separate the product on scale. Finding a good crystallization strategy is so satisfying, recently brought a process up from 50 % yield to 65 % yield, purity after synthesis of 99,98 % instead of 98,00 %, just by slight adjustment to the crystallization conditions. It takes a lot of work to develop in the lab, but the pay off is huge.
@alexreidzero1357 Жыл бұрын
@@habboUdviseren ?
@chandrakantasarkar7556 Жыл бұрын
Are you really a chemist.can u answer one of my questions do we get to study mechanisms of inorganic reactions in higher studies chemistry.i was not really intrested in chemistry till 10th standard but when mechanisms of organic reaction came in class 11th and 12th it fascinates me.but when I start to read inorganic I feel the same when I was in class 10th.so I was asking do wet get to study mechanisms of inorganic reactions in higher studies???? Pls answer🙏🙏🙏🙏
@stefangadshijew1682 Жыл бұрын
@@chandrakantasarkar7556 Hey there :) Mechanisms make sense if you look at the movement of charge and atom groups in molecules, so you will find them a lot in organic and metal-organic chemistry. Inorganic chemistry is often better described by other models, but those get just as interesting. Inorganic chemistry gets more interesting in university, I don't know how far you will go with that in High School. Hope you have fun in class. :)
@slimp4644 Жыл бұрын
Nice video and showcase of click chemistry! When I run a column, I always load my sample with the tiniest amount of DCM or Acetone to avoid having it crash out of the solution, otherwise nice job!
@angelarredondo1016 Жыл бұрын
I use click chemistry to couple PEG to MOF drug carriers, where the azide of a organic modulator bound to the MOF reacts with a terminal alkyne on PEG. Really cool and easy stuff that you can monitor with FT-IR
@I_XuMuK_I Жыл бұрын
If you have an access to enough tlc plates you can try finding your own eluent for the column using other solvents. Maybe using acetone seeing it's good at dissolving the product.
@IanHaltom Жыл бұрын
Yep. Have always done preliminary tlc before running any column. Saves time, prevents wasting solvent, and of course you then know what you're looking for when you check all of your eluted fractions on tlc (using the same solvent system)
@kel000001 Жыл бұрын
Acetone is definitely not what you should use, it will dissolve the silica and come out with your product. Dry loading is what you should do.
@quantrox8191 Жыл бұрын
Don't even need to have a lot of plates. Cut the plate very small, use a very fine spotter and if done with care will be enough to see if the separation is enough or not for given solvent polarity. And definitely, doing tlc before the column is a really good idea if there is no information which eluent should be used
@I_XuMuK_I Жыл бұрын
@@kel000001 If using a lot of acetone - sure. But if it is used as a component in not so polar mixture everything should be fine. It is in the same selectivity class as ethyl acetate so it should give somewhat similar results.
@nitrgnlab9400 Жыл бұрын
@@kel000001 still it is easier to separate the product from silica than running liters of eluent. Dry loading is not the best choice if the product and the impurities have similar rf
@fcsolis Жыл бұрын
Great! I remember all the endless column chromatography I had to perform in graduate school. So much work for so little yield. Thanks.
@IronTeddyBear Жыл бұрын
You and me both.
@kaischapen3552 Жыл бұрын
Yaeh, me too. Especially nasty during summertime, when the heat and the vapour really make you dizzy :-)
@bencetoth9491 Жыл бұрын
Advice for column chromatography: Layer number 1: cotton wool Layer number 2: sea sand for flat surface Layer number 3: slurry of silica gel Layer number 4: pack the silica gel Layer number 5: sea sand (flat surface is required for good separation) Layer number 6: add the dilluted crude product with glass pasteur pipette (easy to clean) and use circular motion with your hands to evenly distribute the diluted crude product (gently add the diluted crude product to do not mess up the evenly distributed sea sand surface) Layer number 6 alternative: you can use celite as the carrier (flat surface is required for good separation) Layer number 7: another sea sand layer (flat surface is required for good separation) Gently add the eluent mixture to the column and have fun.
@visiblur Жыл бұрын
One of the recipients was my Ochem professor. He was so excited about this, even before winning. The party we threw for him at UCPH was amazing. Great professor
@paulmatamane3397 Жыл бұрын
Absolutely remarkable, Nobel prize well deserved on this one
@swapnilpradhan9543 Жыл бұрын
This is by far the best video I ever watched on KZbin, thank you very much for your explanation. Best explanation of organic chemistry work.
@swastikbiswas8293Ай бұрын
I loved the trick of washing the isolated compound laden with coloured impurities with insoluble solvents... had it been me I would have did another column chromatography again!
@jimparsons68033 ай бұрын
Nifty and thanks. Heard about this approach. As a chemist that has dabbled in polymers, this sounds sort of familiar, as the stated objective often is to make molecules (usually up to about 5k or so monomers) of repeating monomers. Well done, altogether.
@lablulz2483 Жыл бұрын
Cool vid! My understanding is that part of click chemistry's appeal, is that it tends to avoid the more fidgetty workup procedures like chromatography. Maybe simple recrystallization of the reaction extract residue would work?
@Chemiolis Жыл бұрын
I have seen an easier purification method when they simply used an expensive Cu(I) catalyst, but for what I did I only saw them doing column so I just followed that. Maybe there is a better way to do this, but I don’t have the time to experiment on it.
@brannigandupreez2083 Жыл бұрын
This is one of the best videos of practical organic chemistry I probably ever saw. Click chemistry is cool.
@gradboften Жыл бұрын
in circumstances where your crude material does not completely dissolve in your eluant, it often helps to load your material onto celite or silica, by taking a solution of your crude (in e.g. acetone), forming a suspension with the celite powder, and then removing all solvent under vacuum (with or without heating). You can then add the resulting free-flowing powdered solid directly atop the bed of sand at the top of your column
@kkgt6591 Жыл бұрын
There are so many chemicals and steps involved. How were these chemicals produced before the click reaction was invented? Also there was so much heating involved and not at room temperature.
@giovannipelissero18867 ай бұрын
Morten Meldal is my MSc professor, great teacher and very very knowledgeable in synthesis. He came up with the "hand rule" that takes advantage of the fact that our hands are chiral, let me tell you it's sooo useful! You can go from a very complex conformation and spatial disposition of the reagents and rewrite the structure while keeping all the chiral centers correct. It saves so much time and mental energy. The hand rule alone could be enough for a nobel prize 😂
@daikucoffee5316 Жыл бұрын
RIP vacuum pump
@666ksmith2 Жыл бұрын
Love your channel, keep up the great work 👍🏼 👏
@dopa_explainz0080 Жыл бұрын
i want to make Methamphetamine through click chemistry, please help me synthesize it!
@crabcrab2024 Жыл бұрын
Yeah! I can already see that in the news: “DEA found out that Nobel prize winning chemistry CAN be used to synthesize METH!!! The agency calls for immediate BAN of click chemistry in ALL of the US!”
@carelessanonymous65704 ай бұрын
I got to meet Dr. Sharpless today. He gave a lecture at my university talking about his work developing treatments for tuberculosis using Sulfur flouride exchange.
@fluxqubit Жыл бұрын
Wonderful work. Not a chemist, but it is nice to see a nobel creation experiment as it runs in the lab. Thank you!
@nomcopterlabs Жыл бұрын
Super cool reproduction! What do you use to protect your vacuum pump from the solvent vapors? Or is it a water aspirator?
@Chemiolis Жыл бұрын
My regular pump is a diaphragm pump, teflon internals, so it can handle pretty much anything going through it. For high vacuum applications I use a rotary vane pump, but then I use a cold trap.
@nitrgnlab9400 Жыл бұрын
@@Chemiolis don't be so sure, even teflon coated diaphragms swell and die after 2-3 years. Personal experience
@bromisovalum8417 Жыл бұрын
For such work I've used a refrigerator compressor as cheap vacuum source. They're very cheap, free even (dumpster diving, recycling plants...), they pull a similar vacuum to a water aspirator (50-60 mmHg, I've seen some as low as 30 mmHg) although the flow rate isn't stellar (just takes some more time to reach peak vacuum). And they can take a surprising amount of abuse. Solvent vapors, acid fumes... sometimes for years. When it dies, you can always get another one. Oh and you can even run lengthy vacuum distillations with it, just put a fan on the pump for cooling and it will run for 12+ hours.
@LiborTinka Жыл бұрын
Could this be used in the preparation of BOP or PyBOP (peptide coupling) reagent?
@That_Chemist Жыл бұрын
the synthesis of BOP/PyBOP/HATU is a really good idea for a video
@ChemistryProfessor_unStoned Жыл бұрын
Great video! Very informative 👏
@guisimoso5 Жыл бұрын
Awesome as always!!!!!!
@joshg.6536 Жыл бұрын
Nice Video Mate and very interesting!
@microwave1119 Жыл бұрын
I think on a technicality this is a “copper-catalysed azine-alkyne coupling” rather than a click?
@stevestarcke Жыл бұрын
Great video. I love chemistry but if this is "click" i would hate to perform "normal"
@jacob9673 Жыл бұрын
Wait why? This ran pretty smoothly. (outside of the slow column)
@frankmercer7009 Жыл бұрын
Very good video. I did my graduate work on organic azide chemistry. I liked you video presentation very much.
@IronTeddyBear Жыл бұрын
This is a really cool reaction, and I appreciate all the detail you took creating this video. I wonder how easy it is to release the tosyl group to add other substituents to the triazole ring. Also, this looks like there is a bit of Diels-Alder effect causing this "snapping into place" that's aiding the facility of this reaction. Pure chemistry doesn't get a lot of love these days from the Nobel committee.
@asensniper Жыл бұрын
You should try loading your crude with a solvent you know is going to dissolve your crude (i.e. DCM/Et2O) with a minimal amount. Then flush your column with 100% hexanes (DCM/Et2O) gets taken away, only until then you can start flashing with your desired eluent
@ejkozan Жыл бұрын
i think here better way would be dissolving mix in DCM and evaporating it with siica gel to coat it, then pouring dry silica onto column and eluting everything
@asensniper Жыл бұрын
@@ejkozan that is a great alternative, however dry loading isnt alwais ideal since some product will.alwais adsorb permanently on silica and never elute. I alwais found my yields to be lower when dryloading vs when I dont, but it has its applications!
@EthnobotanikFAQ Жыл бұрын
@@asensniper that’s why you should use Celite
@ejkozan Жыл бұрын
I agree, it depends on separation. As for celite, i found it can decrease flow a lot. I prefer to use coarsest silica i can get, for graffiti separation or even bigger particle one
@nervonabliss Жыл бұрын
@@ejkozan Have you ever tried a 80/20 mix of celite to silica to improve flow?
@user-yb2vt2xg4i Жыл бұрын
That's pretty cool. Could I transmit this video to a Chinese video website (bilibili) with a translation attached?
@spiderdude2099 Жыл бұрын
I use this technique in my lab all the time. We've engineering a protein with a non canonical amino acid with an azide allowing it to be coupled to an alkyne linker
@ArjanD78 Жыл бұрын
Mooi man! Dit ga ik zeker in mijn les in 6V gebruiken. Top!
@scrotiemcboogerballs1981 Жыл бұрын
Thanks for sharing
@wissen5410 Жыл бұрын
Sorry but my Prof says ist was Noting new and from what i read it was the active Youse in biological Sythems what was Winning the Price Not the Reaktion ist Self
@Chemiolis Жыл бұрын
Both the inventors of the reaction and the inventor of the modification for biological systems won the prize. It has been in use for many years now, so yes it is not new, but that is not a requirement for the prize.
@richardwiersma Жыл бұрын
Deutsch bitte
@anonymizationoverload9831 Жыл бұрын
@@paradiseexpress3639 "Sorry but my Prof says it was nothing new and from what I read it was the active use in biological systems that was winning the prize not the reaction itself" So basically, from what OP read, the thing that got the prize was the use in biological systems and not the reaction itself + the prof said it wasn't a new reaction, even though as Chemiolis said both the original reaction inventors AND the one that discovered the bio-applicable modification got it :)
@nitrgnlab9400 Жыл бұрын
@@Chemiolis except Valery Fokin who actually developed this reaction
@littlehills739 Жыл бұрын
well would be nice if u explained how it would be used and the imprecations of it
@IronTeddyBear Жыл бұрын
Lots of pharmaceuticals have triazole rings.
@VideoMakesMeHappy Жыл бұрын
Subscribed!
@elvedin65 Жыл бұрын
How to you determine the amount of silica gel you need for the column?
@Chemiolis Жыл бұрын
Depending on how good the separation is on TLC, you multiply the amount of g of product you want to purify by 20 for very easy separation. If the separation is very difficult then 100x. Anything else somewhere inbetween.
@xanshriekal Жыл бұрын
The workup and purifications required for any sort of synthetic chemistry are why I'm a computational chemist.
@bromisovalum8417 Жыл бұрын
practice makes perfect
@emlakes Жыл бұрын
Barry Sharpless has already won the Nobel Prize for the same subject, I believe. Can anyone explain the differences between the two prizes?
@riturajsinghpanwar6470 Жыл бұрын
Thanks
@brettmoore31949 ай бұрын
Could you show a non enzematic way to make 1 andostenediol or 2 andostendiol? Im really looming for the process of changing its locality🎉
@maskedmarvyl4774 Жыл бұрын
The number of steps involved in this is dizzying. Where they all Really necessary to obtain this product? Given the length of time involved, and the number of chemical processes to obtain this final product, I'm missing the advantage that "click" chemistry is supposed to provide.
@sudiXP Жыл бұрын
Well done.
@CartwAalbiel Жыл бұрын
I really regret not choosing chemistry in university, would have really liked to do this as a career. Anyway, fun to watch!
@brannigandupreez2083 Жыл бұрын
Can still be done. You're never too old to do so.
@CartwAalbiel Жыл бұрын
@@brannigandupreez2083 Thank you for your kind comment. I know you're right on principle, but things are a lot harder and more complicated when you're older. Still, maybe I can keep that in mind as a goal...
@selfactualizer2099 Жыл бұрын
everyone has more than one thing they want to do. society just doesnt really let us since the world is so competitive.
@frysebox1 Жыл бұрын
It's all fun and games until things like 5:10 happen and you spend hours upon hours in the lab trying to unfuck the situation, with 10% yield and a dubious NMR spectra to show for all of it
@C134B Жыл бұрын
how about the tetrazole producing ugi 4CR?
@MCDainter Жыл бұрын
Nice video ❤️
@defaultname992 Жыл бұрын
I was hoping you would just mix two things and get the new product. I think there is another Nobel prize waiting for someone who would actually make this process much simpler.
@RuthMorsosa Жыл бұрын
The click reaction starts at 2:44 and ends at 3:46, before he was making the reactants and after he was purifying the product
@arsenehanger7456 Жыл бұрын
I thought you have to use a reagent like TBTA to protect the Cu(I) catalyst for a complete reaction. Otherwise it would disproportionate to Cu(0) and Cu(II) which means your catalyst is not active anymore. Thumbs up if you agree😅
@e_gorrr Жыл бұрын
Cool video!) Remeber how I made some tetrazoles from sodium azide and nitrilles)
@Tarquin2718 Жыл бұрын
But what can you use the created compound for? And is this the best way to make it?
@ahmedaldoori6221 Жыл бұрын
Dude you are amazing
@bikrampaul66507 ай бұрын
How much amount (equivalent) of catalyst you have used?
@ugarit5404 Жыл бұрын
I think butanone would be a better solvent,being more stable- amazing vid btw
@ibrahim-sj2cr Жыл бұрын
would it be a problem that butanone doesnt mix as well (obviously) or high BP (80 degrees) ?
@chrisX1722 Жыл бұрын
Did I miss the explanation of the mechanism?
@orca_ah Жыл бұрын
Hey, but we still need so many solvents, etc. I think then it's not a very green reaction? Or there was a reaction before for this product which was not green at all even worse than this one
@ph1ss1ks93 Жыл бұрын
Hearing the words column chromatography gives me PTSD from my silica layer cracking 5 TIMES IN A ROW. But as a Chem major I love watching these videos
@mani-rn1xh Жыл бұрын
What is low radiation EMT magnetic oil?
@AndreZoiaum Жыл бұрын
we did that reaction to produce a 5 membered ring. I remember it was a slave drive and it made me change to plasma chemistry lol
@felixbums624011 ай бұрын
Man thats why i hate column chromatography
@benzen3179 Жыл бұрын
what kind of vacuum pump do you have? I mean the model
@robina6645 Жыл бұрын
Why is acetone added to the reaction mixture? Acetone is know for reacting with itself and forming tautomers right?
@Chemiolis Жыл бұрын
The aqueous acetone is just the solvent
@experimental_chemistry Жыл бұрын
7:28 "still very simple"... 🤭
@Simonsays7258 Жыл бұрын
Can you make LSD like that?
@akramraza8361 Жыл бұрын
Why dont you use cu+1 directly, ?
@Chemiolis Жыл бұрын
Many Cu(I) salts are air sensitive, there are some organic ones that are very good for this but they are very expensive.
@kkkkk3212 Жыл бұрын
Perfect opponent of nile red😂💕💕
@anujith9697 Жыл бұрын
Man that was fun
@tongbunsing Жыл бұрын
What’s the advantage of this molecule?
@a3mia3mi82 Жыл бұрын
I was going through some comments and understand one thing that lot of non chemical or less chemical background folks are here watching video. For non chemists, the name is actually missing that they thought click chemistry is like a Lego pieces snapped together, they raised concern about why to many chemicals and heating involved etc. if you know the actual process chemistry, then we understand the simplicity of this click chemistry and again in my opinion, it can be named differently.
@johnl7524 Жыл бұрын
Why u didn't use rotavaper?
@Chemiolis Жыл бұрын
If I had one :)
@cspicer77 Жыл бұрын
Do you do this in a home lab?
@thedude7319 Жыл бұрын
Jonge, wat een efficiëntie
@ryhol5417 Жыл бұрын
I effing love this.l concept. First time
@californium-2526 Жыл бұрын
Nobel Prize chemistry. I like this.
@RoastCDuck Жыл бұрын
Click chem sounds like combining 2 lego pieces without glue. Sadly it dosn't work that way.
@intheshell35ify Жыл бұрын
Voodoo and witchcraft (I'll stick to v=i*r and a oscope) but very cool tools.
@loganmichaels6853 Жыл бұрын
dawg what just happened
@fmdj Жыл бұрын
I wish I could understand the significance of this but... not there yet :) Cool video though, I presume :)
@yourlocalscribe948 Жыл бұрын
Instructions unclear ended up making crack in my basement.
@scienceandmind3065 Жыл бұрын
The idea is worth the Nobel price but the way it "clicks" is far inferior to biochemical bond breaking/formation.
@Friholio Жыл бұрын
Gotta get this guy some air pressure for his columns!
@mmmhorsesteaks Жыл бұрын
One of the postdocs during my master's once ran a click reaction in Jack Daniels on a bet. Got something like 99% yield ^^ Didn't drink the JD after tho.
@nitrgnlab9400 Жыл бұрын
I remember Phil Baran showed one of his reactions which proceeded smoothly in different types of beverage (including JD) at one of his conference presentations
@kenopyowo Жыл бұрын
Quantum Entanglement in Action: The Physics Behind the 2022 Nobel Prize
@robinhidam2868 Жыл бұрын
Molecular formula of chocolate?
@davidvaca6593 Жыл бұрын
Where is that accent from?
@karolus28 Жыл бұрын
Cool
@jordan4786 Жыл бұрын
no... but you did a good 42 brother
@OmerFlame Жыл бұрын
This dude has sodium azide?! Damn, you're scaring me more than Nigel
@crabcrab2024 Жыл бұрын
NaN3 is actually nothing special, man. You should not be scared at all.
@hnz4339 Жыл бұрын
Is this organic chemistry?
@DoINeedAHandle Жыл бұрын
4:15 looks so much like projectile vomiting
@kapynm8591 Жыл бұрын
Hype
@UnitSe7en Жыл бұрын
Simple!
@hinz1 Жыл бұрын
Doesn't deserve nobel price, if it requires column chromatography ;-D