How to Use a UV-Vis Spectrometer

[@sisacperu240]

What happens if I change the value of the threshold? the peaks in the minute 7:52

[@1light4love]

holy crap.... only 4 yrs later, and our spectrometer is prolly 1/100th of that size!!! craaaaazy days

[@mohdmaaz-eo3uv]

kzbin.info/www/bejne/f3fMY3WGrdmrl8U

[@amreshkumar-db4li]

It was a very helpful video. thank you.

[@mohdmaaz-eo3uv]

kzbin.info/www/bejne/f3fMY3WGrdmrl8U

[@ianlee5812]

Can this software be used for UV-Vis-NIR spectroscopy as well as UV-Vis spectroscopy?

[@themindstorm9947]

At 6:34, is number 7 the blank and number 1 the sample?

[@SupremeScience]

Yes!

[@SupremeScience]

This way you can subtract the absorbance of the cuvette and blank solution from the readings

[@RazaKhan-hn6oi]

welldone excellent presentation

[@hrithikababber6718]

What is in cuvette 7? Water? Do you calibrate it before?

[@irisikey]

this video is very helpful. thank you. please which software do you use and what is the manufacturer of this machine?

[@osamabarja8471]

have you ever used a lambda 25 instrument ? i have some question about using it

[@sylwyu1]

Great video, but I don't understand why you didn't add the y coordinate as well on the peak (not just the x). What harm will that extra piece of information do?

[@SupremeScience]

No harm! Just not useful information in this case.

[@mohdmaaz-eo3uv]

kzbin.info/www/bejne/f3fMY3WGrdmrl8U

[@syeda6936]

One cuvtte for sample while second one is?

[@KasulaGopal-k2r]

very helpful

[@JaswinderSingh-bh1gr]

Can u plz let me know UV Vis spectrophotometer and UV Vis spectrometer is same or different..?

[@sruthisruz9943]

Spectrometer is a part inside spectrophotometer. I think they are same..

[@varence7007]

How do I turn on the monitor?

[@varence7007]

Help! I've fallen and can't get up

[@mohdmaaz-eo3uv]

kzbin.info/www/bejne/f3fMY3WGrdmrl8U

[@nareshvarma826]

I want to calibration video

[@mk-daysai4330]

thnx hommie

[@JaswinderSingh-bh1gr]

Can u plz let me know UV Vis spectrophotometer and UV Vis spectrometer is same or different..?

[@frankreiserm.s.8039]

You ask so many questions.

[@SupremeScience]

How's that?

[@tiny_toilet]

@@SupremeScience See?

[@SupremeScience]

@@tiny_toilet ;)

[@sukieyakie]

no taring??

[@kaushikumarihami1982]

awsome

[@iankim748]

Mih Snell AP Chem- 2019

[@tsoekmanyiu8580]

Just wonder if anyone own spectrophotometer in home😂

[@chell6022]

Amobea sister music.

[@sierraboga7223]

clearly forgot basic lab safety and chemical handling...never put the stopper on benchtop

[@SupremeScience]

Seeing as the container needed to be open and two hands were required for the transfer - and the fume hood is a safe place to set a stopper ... Where would you put the stopper?

[@sierraboga7223]

The video is great, that is my only concern is the placement of the stopper. It is definitely possible to hold the stopper between 3rd and 4th fingers if the stopper is a little smaller, it would be much better however, considering the size of the stopper to use 2 or 3 of the kimwipes (that are right behind the stopper on the benchtop) and place the stopper on the kimwipes. Yes the fume hood is a safe LOCATION but the benchtop (even in the fume hood ) is not. There is no knowledge of whether or not someone that worked in there before hand wiped down their work area. Say they were working with something particularly volatile or even something that even a small quantity could cause a chemical reaction with the solvent being used (you never know what was in there!).... now it has been picked up on the stopper and placed in the solvent container.

[@sierraboga7223]

Just s recommended procedure. It is such a common bad practice in labs to think that things are "clean" and wont contaminate anything, but you never know! Its always better to take extra precautions (creating a barrier between the benchtop/workspace and the items that come in contact with housed chemicals).

[@SupremeScience]

Given all surfaces you work on are cleaned by the person before you, you would have a reasonable expectation that there wouldn't be significant interaction between the solvent and whatever may be on the bench top. Ideally there would be nothing, but that isn't practical. Unless you are using extraordinarily sensitive chemicals in super low concentrations, and are very concerned about background contamination, there is really no issue. Whatever your sample is, it is going to be many orders of magnitude greater than background contamination. Just the dust in the air contaminates the sample significantly more than the stopper sitting on the clean bench top.

[@SupremeScience]

Also kimwipes are expensive as heck!

[@alexplorer]

Real women have cuvettes.