explanation starts at 2:14 if you already have a grasp on what antibodies, enzymes, and conjugation are
@michaelhammonds7359 Жыл бұрын
Dr. Hammonds: Thanks, you took a complex idea and made it much more simple and easier to understand.
@mbalenhledlamini26329 ай бұрын
10 years later and this is still the best explanation I have found. Thank You!
@maddieharman83779 жыл бұрын
THANK YOU!!! Thanks so much for making this video! My classmates and I were so confused about competitive ELISAs and this video really helped clear things up for us! We really appreciate your help!
@Daniel47yes8 жыл бұрын
BEST EXPLANATION EVER! Te pasaste flaco, grande!
@leabilicic63842 жыл бұрын
Simply the best video about competitive ELISA I have ever found on the internet :D
@jonathanmartinbenitez74274 жыл бұрын
THE BEST EXPLANATION BY FAR
@alexgalvezmorante96119 жыл бұрын
This video has helped me a lot. Thank you for your work.
@DaLokNessMonster6 жыл бұрын
very clearly explained! the illustrations really help to get the information across!
@kateillac9 жыл бұрын
this was a great, easy to follow presentation. thank you so much.
@kateillac7 жыл бұрын
Just Observing what an original comment, you must find yourself to be so funny. Gender jokes are outdated dude, move on.
@bobby-joe80637 жыл бұрын
Katie Ritchie you on tinder?
@basetwahiz11365 жыл бұрын
Very helpful, i have been searching the chemistry behind it, you made it really easy thank you
@julitequenum1128 Жыл бұрын
This is great. Simple explanation. Thank you so much!
@angryblackwomanphd5 жыл бұрын
YOU JUST HELPED ME UNDERSTAND MY RAW DATA! THANKS!
@nielp918 жыл бұрын
Better than the video my super gave me when I started working. This is on point!
@sarahe.s72315 жыл бұрын
Big thanks.. Praying for you of success forever 🙏💙
@bakeezawzaghlool11 жыл бұрын
Thanks a lot helped me with the topic , I have an exam tomorrow :)
@janejordan8511 жыл бұрын
Very helpful! :) Thank you from Italy!!
@NGOCNGUYEN-df8jk7 жыл бұрын
this video saves my life thanks a lot
@ScheveSneeuwSchuifSchep4 жыл бұрын
Thank you so so so much, this is really saving me for my exam for bioanalytical techniques tmorrow
@skyisnotblueskyisblue23027 жыл бұрын
thank you so much for this!!!!! i was really confused about how competitive elisa works
@sam_liston982 жыл бұрын
perfect explanation man, keep going👌
@Hamlet1374757 жыл бұрын
pretty straightforward, good presentation, i liked it!
@abdul4boys3 жыл бұрын
Thanks a million, the video was very helpful
@056vatsalapandey33 жыл бұрын
thank you so much (tears in my eyes)
@daviejeon10 жыл бұрын
Thanks for the video. It was easy to understand.
@KanyesigyeGeofrey Жыл бұрын
???
@siawweilai24143 жыл бұрын
Thank you sir for the clear explanation
@Daniel-cj1pt8 жыл бұрын
thx my friend very good informative and clear video!!
@shoootingstarss8 жыл бұрын
this is so clear. thank you
@yourfuturedocburenbeiya5 жыл бұрын
This is awesome!! Thank you so much for this video explanation!!
@nwilczyn7 жыл бұрын
the best competitive elisa vid I have seen... still, it is hard to keep this variation on the elisa process straight in my head (grrrr)... any tips or mental tricks?
@yelenasofiapajaroesquivia36346 жыл бұрын
THANKS!! Excellent explanation!! it helped me a lot!
@mustafatalib23673 жыл бұрын
It is quite informative thank you for this effort
@rajyalaxmikonathan8324 жыл бұрын
It was very helpful,thanks a lot
@akasprime11 жыл бұрын
very clear !! ...excellent video
@brijeshverma46776 жыл бұрын
Thank you so much. This was really helpful
@godfreykamugisha40338 жыл бұрын
Thanks for your good clarification
@jjing_jjing_tta5 жыл бұрын
Thank you so much. I could understand clearly!
@alaaal-maliki623211 жыл бұрын
Love all your work and your tutorial!!! Keep it up ;)
@nazminizarimi20308 жыл бұрын
thanks for this video. helped me a lot too
@anumsiddiqui39864 жыл бұрын
When we added the reagent to our sample why not all the GOI get tagged by HRP enzyme..and when we add sample to the plate what are these non tagged antigens that binds with antibody...I m not getting this point?
@Khaledf2 жыл бұрын
Thank you. BUT Why is it used competitively? Wouldn't be more easier to use direct or indirect or sandwaich to detect the interest antigen?
@retomatinho5 жыл бұрын
THANK YOU. I really needed this explanation, I was very confused lol
@GordonChao05088 ай бұрын
Does Competitive ELISA first coat antigen to well plate or antibody?
@GK09011 жыл бұрын
So helpful! Thank you so much!
@sakshipant94416 жыл бұрын
Easy to understand thank u..
@AntoineIbanezgio6 жыл бұрын
Sir, this helped me a great deal, my thanks!
@nathanielhogge954810 жыл бұрын
saved my life
@AZ-qx1xd5 жыл бұрын
Thank you! so clear..
@charityubani18888 жыл бұрын
Clearly understood
@1627anat9 жыл бұрын
Thanks!
@zinaadams23893 жыл бұрын
u r amazing, thanks so much!
@mostaphaaaa38 жыл бұрын
so useful thanks a lot
@fukyouisrael11 жыл бұрын
thanks you for the informations
@rayans18328 жыл бұрын
Thank you very much
@QFBPLR2 жыл бұрын
Está genial tu explicación, gracias (Y)
@nwilczyn8 жыл бұрын
my question is, if I run out of milk for my breakfast cereal, could I substitute BSA?
@madihakaleem88807 жыл бұрын
Its still not clear for me when this experiment would be required. Am I correct in understanding that this can be used to check if a synthesized protein binds similar to a native protein by the antibody?
@Protocolplace-about7 жыл бұрын
Sure, it could be used like that. But competitive ELISAs are most often used to simply measure the amount of a certain protein or reagent that is present in an unknown sample.
@elia182311 жыл бұрын
Thank you
@spicyotato79407 жыл бұрын
Very helpful, thanks :)
@sashathomas5810 жыл бұрын
With reference to RuNs comment " alot of this is unecessary as people will know what an anitbody is" THIS IS RUDE and so very untrue, im glad he illustrated this as sometimes I get the two mixed up ie, antigen and antibody. I was wondeing if you could have videos on competative radioimmunoassay or would you say that the competative elisa is based on the same principls? very good video thanks xx
@severerevenge85754 жыл бұрын
Greatest
@nasira.423210 жыл бұрын
thanks
@sand86837 жыл бұрын
Wonderfull!!
@sajs77785 жыл бұрын
شكرا جزيلا
@fatdah81407 жыл бұрын
Merci beaucoup
@lelawati2310 жыл бұрын
If competitive ELISA can be used to detect antigen, when to use competitive ELISA and when to use sandwich ELISA?
@Protocolplace-about10 жыл бұрын
In my experience, competitive ELISAs take a little less time, but could be less accurate / sensitive than sandwich ELISAs, and could be more prone to user-error than sandwich ELISAs (especially on the step where you add sample / enzyme conjugates to the competitive ELISA plate). But the reality is that nowadays people typically buy commercial kits from companies and use them no matter what type of ELISA they are, and most kits seem to work pretty well. It usually comes down to price and availability of the kits/reagents that you need to detect whatever it is you are trying to measure.
@lelawati2310 жыл бұрын
Thank you !
@reddishcat110 жыл бұрын
It is not really needed to bock the plate with BSA or detergent. noting is really going to go wrong. except if it has any influences on a well ratio transfer. what it does not in my opinion.
@Protocolplace-about10 жыл бұрын
Thanks for the comment. If you could define "well ratio transfer," that would be great. That's a new term for me. My understanding is that if you don't block the plate, enzyme conjugate could bind to the plate instead of the capture antibodies. Then, you would be measuring a false signal, making you think there is less sample protein of interest than there really is. As far as I know, the Competitive ELISA will only work well if your sample's protein of interest competes with the enzyme conjugate for a limited number of capture antibodies. Without blocking the plate, both of them could potentially bind the plate instead of the capture antibodies, undermining the assay. Is my reasoning correct?
@reddishcat110 жыл бұрын
Thanks for your reply. I ask my teacher today before reading this ,and you are completely correct. with "well ratio transfer" I just meant "would the ratio(conjugate/sample) get's good across on the antibody's?" I first thought that this ratio would sit on the antibody, but also on the plate, without any consequence. I was wrong. But not completely, you could get a somewhat right result without blocking the spots it is not really trustworthy, but still more trustworthy when you do the same to another ELISA kind anyway, you make great videos. I like the clear simplistic way they look.
@greencard12452 жыл бұрын
One like is not enough :-) Even I understand you well! Thanky you :-)
@aksharabachu23754 жыл бұрын
noice work
@ÁgataAdriánPérez Жыл бұрын
happy happy happy (voz de pito)...
@cformerly10 жыл бұрын
A lot of this is unnecessary, most people coming for this will already have knowledge of what an antibody is etc.
@Protocolplace-about10 жыл бұрын
Thanks for the suggestion. You have a good point, but I'm trying to help people from all sorts of backgrounds understand these concepts, and some may not be as familiar with the key terms as others. Having listened to many scientific talks, I usually appreciate it when people give a brief review of the basic terminology before moving on to more complicated stuff. It just helps everyone get up to speed. In any case, I hope you benefitted from the video! Best of luck to you.
@xDomglmao8 жыл бұрын
helped a lot and please stick to your manner of explaining, it's awesome what I don't get: isn't it possible to get wrong results, e.g. by any chance many more conjugates binding, than proteins binding leading to a wrong guessed ratio?
@josh3658edwards7 жыл бұрын
You are assuming a lot. The background info is appreciated :)