The Enzyme Linked Immunosorbent Assay (ELISA)

The ELISA, or Enzyme-Linked ImmunoSorbent Assay, is an analytical biochemistry technique that uses antibodies to detect the presence of specific biomolecules (i.e. peptides, proteins, antigens and hormones) in a complex sample. These samples can be single proteins or complex mixtures like cellular lysates.
In this video, we will show you the basics of how to perform an ELISA in your classroom laboratory.
Check out our newsletter featuring the ELISA!
www.edvotek.com...
www.edvotek.com...
www.edvotek.com...
www.edvotek.com...
www.edvotek.com...
© 2015 Edvotek Inc. All rights reserved.
www.edvotek.com

[@timothyow3248]

Background music is very upbeat considering someone has just tested positive for HIV.

[@boshra2006]

yes

[@muhemensamir6369]

The video proved a person with HIV؟؟؟؟؟

[@StephJ0seph]

lol

[@KamiltheCamel]

@@kerrileelawrence2402 Please tell me you are joking? Cancer isn't even a virus that you would make in a lab. Most animals can develop cancer. It is just part of a body aging. Each time your cells replicate the DNA gets mutated a little. Sometimes these mutations happen in important places. The cell can then become cancerous because the genes needed to regulate cell growth are no longer working properly.

[@Graffitus]

@@KamiltheCamel I don't see the original comment, but I just wanted to mention that there are some cancers that are virally induced; in fact, HIV can induce lymphoma in some patients. Human papilloma virus can rarely induce cervical cancer in women. Even in animals we see virally-induced cancers; avian leukosis virus (a retrovirus, like HIV) can induce lymphoma in chickens. *Most* cancers are spontaneous, this is true, but there are some infectious cancers, too! For example, the esophageal worm in dogs, Spirocerca lupi, is a parasitic worm that can induce cancer. And both dogs and tasmanian devils have a type of tumor that is directly contagious via contact. For dogs, it's a tumor called the transmissible venereal tumor (TVT), and for tasmanian devils it's called devil facial tumor disease. And in humans, the spirochete bacteria Helicobacter pylori is thought to contribute to some types of stomach cancer, too. So yes I agree, for the most part cancer is spontaneous and is the product of accumulations of mutations - but there are some infectious/contagious causes, too. =)

[@rizqirizaldo]

I will probably put the background music in the lab as well, that was a banger

[@EdvotekInc]

💃💃💃💃

[@thejuryofwolves]

Super helpful video, and I liked the background music. It's upbeat and keeps me focused and entertained too.

[@heizeljoycecruz196]

Hi! I am a Biology student. According to my research, ELISA Method can be used to detect Cyanobacteria on freshwater. May I know how am I going to detect the cyanobacteria in the freshwater? What procedure will I use/follow? Thank you so much! your answer will be a big help for us, future researchers.

[@EdvotekInc]

Hi, great question! We at Edvotek have not specifically created an ELISA that looks for cyanobacteria, so I cannot provide a specific protocol. My suggestion would be to start with a search of the literature using PubMed to find a paper describing the assay you want to try, and using that as a starting point for the development of your protocol, including time, steps, and reagents. Hope this helps!

[@christinea5495]

THANK YOU SOOOO MUCH FOR THE VIDEO! I can finally understand this journal article im reading :")

[@alexgunawan98]

Anyway we can learn this and do a easy test like using strip?

[@EdvotekInc]

Edvotek offers multiple ELISA tests for educational purposes, available here: www.edvotek.com/search?keywords=ELISA One strip simulation is available here: www.edvotek.com/S-70 Please note, while most of these experiments use real antibodies and substrates, they are simulations of the diagnostic test (i.e. the HIV strip test does not contain any HIV antibodies or antigens).

[@heshamyahya6266]

the background music was just ✨perfection✨

[@Vishwa2087]

There are very few professional s in India to do Elisa in a large scale. Diagnostic laboratory should take brave step for Covid19

[@emamshaik9629]

Explanation is excellent

[@EdvotekInc]

Glad you think so!

[@sanjaykumar-ew6mb]

Thanks for the nice video dear sir ❤

[@MrTurtleOfDoom]

New to ELISA! I have a quick question: is the color change because the detection substrate has interacted with the "sandwiched" antigen? I'm guessing no color changed means we don't have the antigen we are looking for?🤔

[@EdvotekInc]

There are many different types of ELISA, so the complex that forms can be a little different depending on the specific assay (more info here: blog.edvotek.com/2021/01/14/all-about-the-elisa/). But, in brief, if the complex can form and the enzyme-linked detection antibody is present in a well, it can break down the substrate and produce color or light. If there is no signal, then the antigen-antibody complex has not formed. This generally means the antigen is not present, but there is always the possibility for experimental error which is why it is important to have reproducibility in your data!

[@frankreiserm.s.8039]

Nice, quick video. I am surprised that you can use the same transfer pipet for every well for most steps. Frank

[@rehammohamed8145]

Frank Reiser M.S. Is that right or wrong ?

[@enfyasmim]

He use 6 differents transfers pipets for this demonstration. Visualise this video again and look at the 30 seconds of movie.

[@tati2097]

Frank Reiser M.S.

[@ebhawesh]

@@rehammohamed8145 wrong if he did so

[@eclkt]

Thank you for your education

[@thuhanguyen6440]

Please why need used to the stop solution? Thanks

[@rabiyabasri1895]

Thank you

[@sarahsuper4]

Apparently, the ELISA Method can be used to detect BPA on water. May I know how am I going to detect the Bisphenol A in the water? What procedure will I use/follow? Thank you so much! your answer will be a big help for me!

[@EdvotekInc]

We recommend performing a literature search to find the best protocol for detecting BPA in water. This is a very general overview.

[@diya4939]

best video i saw about ELISA

[@ahaaaihittop7515]

Is ELISA and Rapid antibody test HIV 1 AND 2 both Reliable?

[@nwilczyn]

two thumbs WAY up!

[@praveenrathore315]

Very easily understandable steps nice

[@EdvotekInc]

Thank you so much 😊

[@dewHong]

simple and helpful!

[@khurramshahzad579]

Hi! This test is used for anti hcv detection??

[@EdvotekInc]

There are many uses for the ELISA, which you can research using PubMed or similar article databases. This video demonstrates a simulated test using real antibodies and antigens for people to learn the techniques.

[@khurramshahzad579]

@@EdvotekInc Okkkkk Thanks

[@khurramshahzad579]

@@EdvotekInc One of its use is hcv detection too?

[@EdvotekInc]

@@khurramshahzad579 You will have to do your research to determine whether the ELISA is a clinically relevant way to detect HCV. ELISAs are often used in medical diagnostic assays.

[@sandygamertube9610]

l cant know where will we use the primary antibody and what is the reagent and its function

[@partyp3opl393]

I think the reagent is the primary antibody

[@brandonwoolsey5801]

does the absorbance parallel the concentration? Could they be ran through a spectrophotometer to determine antibody concentration?

[@EdvotekInc]

This will be slightly different for every substrate, which is why we would create a standard curve from samples of known concentration. The absorbance of the standard curve samples would be measured using a spec, plotted on a graph, then the experimental sample compared to the graph. For more info, check our our experiment: www.edvotek.com/266. The lit covers creating and analyzing a standard curve.

[@josettenaadromo959]

please, when do you add your sample if you are to purchase this instrument. Can it be used for detecting viruses?

[@emilie6466]

The assay can be used to detect viruses, but that requires having the antigen of the virus and an antibody that binds to the antigen. Besides, RT-PCR is how we detect viruses nowadays and it is far more precise and accurate in quantifying viral load.

[@EdvotekInc]

This is a simulation that cannot be used to detect viruses.

[@pinksheeptwopointzero]

The Elisa (or the 100 microliter to nanoliter

[@prabhatimishra517]

Background music is bery irritating

[@احلامجبارين-ظ3ي]

thanx 👍

[@okeneemmy4696]

Good lesson

[@agildarmawan4753]

really educative, appreciate it!

[@ArnavPadhi]

What is the KIT used here ??

[@EdvotekInc]

This is a general video which can be used with any of our kits. Here's a few to try: www.edvotek.com/271 www.edvotek.com/267 www.edvotek.com/274 www.edvotek.com/280

[@ArnavPadhi]

Can u plz explain step 6

[@advaitnandedkar2924]

What's the function of PBS?

[@sandygamertube9610]

its a washing buffer used after eash step to prevent interaction with plastic wells as l thought and maybe l am wrong

[@pairotypeter3246]

ITC CUL

[@dustingatzke8714]

Advait Nandedkar pms

[@NARANGI113]

Very helpful😊 Thankyou

[@sivaramreddy8134]

What if steps (provedure) are not followed ??

[@EdvotekInc]

Depending on the protocol and the step you did not follow, you can end up with a false positive or false negative result.

[@piyushsen7502]

it cleared my all confusion tnqu

[@vikasjha2991]

Call me 7045020443 i will share my experience how thyrocare cheat in testing report and how A velumni murdered his wife,,

[@eshetukassa7867]

Tnxs

[@Sally6861]

THANK YOU

[@davidabraham6176]

it was really educative

[@ams8399]

my heart had heart attack by the speed of the video :)).. nice one

[@riyamaurya6129]

Can't we decant the reagent or buffer from the well instead of removing it by the dropper

[@emilie6466]

Riya Maurya you could but what would be the point of that? You risk disturbing the sediment and skewing results. On top of risking cross contamination when you pour it out.

[@Labii]

Labii ELN & LIMS has developed a widget to perform ELISA data analysis directly in the ELN platform with a few simple clicks. Learn more at blog.labii.com/2019/08/elisa-standard-curve-with-labii-eln.html.

[@Swathi230]

What is function on PBS

[@noorulain6066]

it is used for washing to remove the unbound or extra antigen or antibody

[@sandhyasandhya4266]

Background music is disturbing a lot ..m

[@uniteckrao81]

It's very good way

[@uyouyu4158]

great

[@geethamounica1103]

its so helpful

[@dr.sapnayadav4023]

Very helpful

[@Vishwa2087]

Nostalgia, we didn't have youtube those day's.

[@dominiqueskinner1951]

The background music is louder than his voice-over makes it hard to hear him talk.

[@EdvotekInc]

Thank you for your feedback.

[@prasannaprasanna4791]

Elisa reagent name

[@sandygamertube9610]

l wanna ask the same question so please if you have the answer just tell me

[@GovindYadav-kf9ok]

Usually I have to increase the speed of education al videos here I have to reduce it

[@ancientelevator9]

So much pipetting! No wonder there are so many companies working on lab automation!

[@cortexoftheego4568]

"add detection substrate like specified in product" nice advertisment.

[@EdvotekInc]

Hi! This is a video produced by our educational biotechnology company to show how to use our product! Our ELISA kits follow similar protocols but may use different detection substrates. So, while this video is not an advertisement, it is designed to go with the experiments available for sale on our website.

[@amarasaman3370]

Ye kon c type ka elisa perform kr rhy han?? Indirect? Or competitive?

[@syedalaiba4914]

Indirect

[@a.m.g1268]

I think , this is direct sandwich elisa

[@EdvotekInc]

This particular assay is a direct ELISA. However, there are sandwich ELISA experiments for education labs available at www.edvotek.com

[@sh.m.m1663]

i guess there's a device to do the washing

[@ismailsvlog1228]

Longest molecular technique process 😐

[@EdvotekInc]

I can name a few that take more time... Western blots for one! But the information that you get from the ELISA is very powerful in many fields.

[@gabs_uk6432]

Good but useless background noise

[@Christellek1550]

Can someone translate to English

[@ebhawesh]

It's in English

[@Christellek1550]

@@ebhawesh I didn't mean it literally

[@infinitefuture1828]

Lose that background music, and it'll be perfect

[@kenivia]

👏

[@level6271]

ابو قاسم هيييييييييييييه

[@Bamdeidz]

Hardest crafting tutorial ever

[@EdvotekInc]

We do have a crafting tutorial. This one focuses on the ELISA.

[@donajor8]

Hello, I have a question By Elisa there are many positive igg as ebv hhv6 cmv But with negative igm. And negative pcr? What do this mean with clinical symptoms?

[@mattiacardaropoli6922]

It could mean that there was a previous contact with the virus so igg are positive but no infection at the moment cause igm and pcr are negative. Symptoms might be for other reasons maybe

[@nipppoohagoo9092]

Elisa kamu mau jadi sahabat aku kalo mau aku minta nomor hp kamu dong😊😊

[@MaxDiscere]

The video is as mundane as the task of performing such a test

[@EdvotekInc]

Thank you for your feedback!

[@hamidwaqas4708]

annoying background music

[@nipppoohagoo9092]

Elisa kamu mau jadi sahabat aku kalo mau aku minta nomor hp kamu dong😊😊