Competitive ELISA

[@AZ-qx1xd]

This is the clearest explanation of competitive ELISA i have seen thus far, thank you!

[@AM000]

i don't think it's right for competitive ELISA we want to measure the ag not the ab in this video he is explaining a situation where he measures ab instead of ag

[@XDAnImEkRaZe3D]

THE ONLY VIDEO THAT EXPLAINS WELL, SIMPLE AND CLEAR!! THANK YOU SOOOOO MUCCCCCH!!!!

[@pragathiraghavender1843]

Absolutely, the best explanation. ELISA techniques have always been confusing for me. You really made it simple 😅😅.

[@kendall2004]

Alhamduliallah i found your channel,thank you a lot;May Allah bless you.

[@prathz1065]

You really have good ability to teach 👍 Thank You for your effort 😊

[@himanshibhagoria3928]

Amazingly explained and cleared all the doubts through well explanation and the animation...Thanks A lot for this!!!!❤

[@backbenchersbio3688]

Thank u sr Good & easy way of teaching Make more vdos for us on technique's

[@lenonre]

the best explanation of competitive ELISA! thank you! 👏👏👏

[@nishasinghal7375]

this is best explanation of competitive elisa on youtube...thank u so much

[@slguitarists3570]

Excellent explanation🌹🌹💮🌺🌼🌸💮

[@Rajsavaliya1410]

Supar se bhi upar 👌👌👌

[@MirabelleLum]

Very clear and easy to understand Thanks

[@jaydeepdumadiya2464]

Great great great job....🔥🔥🔥🔥🔥🔥

[@shikhasharma0038]

Very beautiful explanation.. Thanku so much for this clarification 🙏🙏

[@strivingforexcellency]

Thanks bro u saved my brain from going insane

[@dina3630]

You are blessed😊

[@sawairagul251]

Well explained 👌🏼... mashallah

[@Kalyanihealthcare]

Very nice 👍👍

[@sumitojha5587]

Sir what happens if no antibodies are present and only the enzyme linked antibodies are attached to the antigen present on the microtiter plate. And colour developed..

[@amnairshad1995]

Excellent Explanation Sir❣️❣️

[@sakhaty1794]

That was amazing , thank you

[@SohamDharmik]

Excellent Explanation

[@jithuraju6503]

👍

[@thesuperbiology5804]

Super and good explanation... Wishes to Know the way that you makes these types of animated tutorial videos

[@teshaletekle6718]

Short and clear Explanation Thanks sir!

[@simplifymlt]

Best explanation 🎉🎉🎉

[@MJ-fx2zu]

👍👍👍👍👍💯💯💯

[@lydiashaji5611]

So helpful. Thank you so much

[@footgoodball107]

Very easy to understand but am wondering why would you capture the competed Ag. And how is the primary Ab enzyme linked. But have also read that there are different procedures for competitive ELISA. So I won't argue. All in all thank you so much for the videos. I really appreciate

[@footgoodball107]

Also the one that is being competed for is the one that is enzyme linked. Not the competitors

[@amritasriv87]

Very well explained ...

[@mudassariqbal6874]

Thanks bro apny mayra concept clear kr diya

[@sajidmicrobiology]

Contact for Personal or group immunology crash course (online) [e-mail]

[@mia5091]

👏👏👏

[@rafiarehman8860]

In this video you said that two types of antibodies are used one label with enzyme while the other is not and they compete with one another. While I have read in research paper that enzyme conjugate antigens compete with sample antigen. And this is tha only difference between indirect and competitive Elisa. Kindly review it again and also guide me regarding this issue.

[@sajidmicrobiology]

If you want detect particular antigen from the sampe, you to use conjugate antigens.. if you want to detect antibodies, the conjugate antibodies will be used

[@rafiarehman8860]

@@sajidmicrobiology ok thanks

[@rafiarehman8860]

@@sajidmicrobiology it really means a lot for me

[@TutunChanda]

Exam saviour videos.

[@Theprofessionalsurgeon]

take all my money man, you deserve it

[@doctorkhan5952]

Awesome!!!!!!!

[@ushakarthikeyan6455]

Super, good explanation

[@MuhammadRamzan-fe4hz]

Very Impressive keep it up bro 😉

[@myjourneytotheendofunivers]

Thank you sir

[@avinashk9966]

Awesome bhai.. thanks for this video. I made a notes out of it. ❤️

[@achintyasasmal4471]

✌️👌✌️

[@sohaylasayed3829]

thanks a lot ☺️

[@mansikesarwani900]

Good explanation

[@vangmayireddy9516]

Plz upload western and southern blotting..by the way..u gave a wonderful presentation...tqsm

[@jiyyadmohammed7604]

Beautifully explained the concepts

[@fatimahayssam120]

Thank you for you effort ❤️

[@sajidmicrobiology]

Thanx... whr r u from ?

[@fatimahayssam120]

You are welcome ,lebanon

[@sajidmicrobiology]

Ok

[@mohammadmasroorzafar9728]

What exact reverse for antigen? Can you explain it?

[@AdityaRaj-zk9eh]

thanks sir

[@nightbot41]

best explanation, thank you!

[@maryamnashwanlaso7567]

Thank you

[@titoflash1212]

Thx a lot

[@vijayachandrakalyani779]

If more colour more we affected ? Or less colour more affected with anti gen?

[@abhijitsarma8525]

Sir, the enzyme linked ab s r taken from where? From the serum sample? Well the non enzyme one is taken from the sample and that's understood, now since we actually wanna know the concentration of the ab, so how can we link the enzyme to those ab s present in serum? Humein to conc. pata nahi hai, so serum se kitna ab lena hai... Kitne ab ko enzyme linked karwana hai.. I'm finding it confusing 😭

[@sajidmicrobiology]

Enzymes linked antibodies redymade hoti hain... jaise blood group detect karne ke liye kits hoti hain.. waise hi ELISA ki kits market me available h...

[@MirriamKatadzuka-x3p]

Nice job sir

[@nurkarimah1123]

Whether ELISA and EIA are the same or different?

[@prabhudasnair5636]

Im subscribing ur channel

[@Panda256]

Dude, thanks. That was helpful.

[@addedvalue9607]

Nicee❤

[@ericadipedeee_6060]

if your looking at the ability for an antigen to outcompete the one with the enzyme...shouldn't you want the concentration of both antibodies to be the same? Or else obviously the one with the higher concentration is going to bind more

[@amolmorrow2799]

you do a whole bunch of wells each with a diff amt of enzyme (known and labled) and then you can just read the tray to see where it started outcompeting and quantify the amount that way

[@ashritpattanayakfan]

Thank you so much, sir 🙏

[@fellahfathima1838]

Why we are testing antibody instead of texting antigen in case of HIV ? We need to find whether there is presence of antigen or not .. right? Pls explain

[@sajidmicrobiology]

HIV antigens are also detected.. in tha case p24 antigens are the target.. in intial weeks hiv antigens are dominant...but antibodies are not found... after 2 to 8 weeks antibodies can be seen

[@Mihiruwan-i2x]

❤Thanks

[@srijitgh]

Nice

[@prabhudasnair5636]

God bless u brother Such a easy and clear explanation by u really u have a great way of explaining Keep it up

[@sajidmicrobiology]

Thank you so much for appreciation

[@divyamogilipala5779]

Sir bacterial vaccins method of preparion video cheyandi plzz sir

[@prabeshtigerdhakal4660]

Can there be equal concentration of specific antibodies and enzyme linked antibodies? And what happens if there is?

[@ankitayadav1123]

I think standard readings or graph are always there to compare

[@footgoodball107]

Interesting scenario. But you are going to avoid that by using more sample

[@hiteshvishwakarma6449]

Plz upload more videos on microbiology

[@sajidmicrobiology]

Which topic ?

[@bharatrawlley6434]

Thanks a lot!

[@sangzz__world]

Thank you❤️❤️❤️

[@tarteelmohammed3257]

Your efforts are appreciated!

[@sajidmicrobiology]

Thank you so much

[@Quotessymmetry]

Sir pls Blood group k doner or recipient smjhain wo nai aj tak smj aya kon kis ko day lay skta antigen or antibodies ka chakar easily smjha dain pls

[@sajidmicrobiology]

Sure

[@Quotessymmetry]

@@sajidmicrobiology ok i will be w8ting

[@zishanahmed2441]

Why high concentrated win competition

[@naeeemali411]

antigen is reacting with both bro whose conc. we detrmine

[@bhaskerreddykoyle3738]

Is Competative Elisa is also called as Direct Elisa.......?

[@sajidmicrobiology]

No

[@bhaskerreddykoyle3738]

Okk thank you sir

[@Szabarmawi]

thank u so much

[@sajidmicrobiology]

Most welcome

[@shaswatraj11]

Damn man , you made it easy

[@travelwithpaalii007]

Strange sounds behind the video scary

[@hoshyarjamal4597]

I think I heard the call to prayer

[@hiteshvishwakarma6449]

Plz inform me if you get my mail

[@khushbuchasiya3075]

Very low sound

[@hiteshvishwakarma6449]

Plz check your mail I had mail my syllabus to you

[@Salaar00]

🫶🤲