ELISA test

ELISA assay - This immunological assay lecture explains about the elisa test procedure and principle behind the elisa assay including direct, indirect and sandwich elisa techniques to determine the presence of antigen and antibody in blood sample or serum sample.
For more information, log on to-
www.shomusbiolo...
Get Shomu's Biology DVD set here-
www.shomusbiolo...
Download the study materials here-
shomusbiology.c...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in KZbin. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.co...
LinkedIn - / suman-bhattacharjee-2a...
KZbin- / thefunsuman
Thank you for watching the lecture video on Elisa test including direct and indirect Elisa assay.

[@Safiro416]

i've been watching your videos since my second year of college, and years later i'm here again to understand more the work i'm doing. thank you shomu!!

[@pakhisingh6378]

Sir we are getting a lot from your efforts

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@divarryyaa790]

Way alot ✨

[@uma8452]

Thanks a lot! I couldn't get a clear fundamental understanding from any online source or journal so far. This has been very helpful.

[@shomusbiologyofficial]

All the best

[@lifaam]

Thank you. Your teaching is so helpful and the best I’ve gotten. You put it in a way that the students understand the concept and that’s the best part.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@nickharrison2916]

Hey shomu, i've only just gotten into your vids this semester. They've been great for helping me with Immunology and Microbiology (lac operon). I appreciate the specificity of your videos. Keep it up!

[@Cherriheart]

This really helped me study for my labs! Thank you!

[@dawnhoran3353]

As always a perfectly clear and fabulously concise explanation thank you so much. I now always go straight to your lectures for clarity on subjects so thank you very much.

[@imanekabouche3720]

This was very clear, it truly helped me understand this procedure better ! Thanks a lot

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@cathyyanagi428]

Thank you for making the concept so much easier to understand. You are a great teacher :)

[@sanyarajput6397]

All concepts crystal clear! Thank you 10000000 times. Very helpful ✨

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@findyourniche1007]

Finally it clears my concept about ELISA😊

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@jasonsharmamusic2925]

Today I have Microbiology exam 2nd prof. MBBS. Thank you Sir shomu for this video. My mind was about to blow up looking at the book.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@tatinikayal9295]

I can't find this topic in clug and Cummings.your video helped me to know this.topic..thank you sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@MrCompenso]

Love your videos man! So clear, not over complicated. I'm currently working an internship at a biotech startup and your channel has probably been more helpful then any other channel on these topics. From the heart, I appreciate it! Keep it up!

[@Theodore660]

Got my exam in 3 days... thanks for this... saved me a lot of precious time.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@jaidevdh01]

Sir i study biotechnology your concepts in videos are very clear and specific thank you 🙂

[@shomusbiologyofficial]

Thank you

[@blossommingupdaybyday6075]

I didn't understand in Ananthanarayan book sir. Ur viedio is very helpful to me. VERY BENIFICIAL

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@fishtailstinkytoe3637]

Shomu is a living legend

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@shreyasengupta2984]

Awsome...explanation..thank you...being a bsc nursing student...i got my doubts even more clear...thank you vry much sir...

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@shreyasengupta2984]

Yes sir...thnk u again

[@siphesihlebongiwe1540]

Well explained. Thank you so much, Mr.

[@shomusbiologyofficial]

You're welcome

[@jemimahrajakumar7770]

Im kinda disappointed that the pics in the thumbnail weren't used but still this video helped me better than any other, thank you bro

[@ahmedeldesouky1878]

your explanation is always great

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@mayukhroy5393]

Wonderful explanation, Sir. Thank you So much.

[@shomusbiologyofficial]

You're welcome

[@mahparakhanam5093]

Thank you for such a good and helpful video...i understood it completely

[@shomusbiologyofficial]

You're welcome

[@abhinavhp9405]

Thanks a lot bro😍😍 ur teachings alwayss come handy for my exams.. The lessons i watch at the last min of exam wil always appear and fetch me marks😍😍 thanks a ton

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@abhinavhp9405]

@@shomusbiologyofficial yesss alot.. You're master of all in this.. Best of all😍

[@gnneerajashree5550]

Thank you so much sir.......I watched all your immunology videos and did my exam today very well sir.....Once again Thank you so much sir.....

[@shomusbiologyofficial]

Glad it helped

[@bubbleminho9092]

Really beneficial 🙌🏻🙏🏻like before I didn’t understand anything 😩Thanks a lot 🙏🏻🤝😃😊

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@ifeanyichukwueke9942]

The illustrative teaching helps a lot! Thanks

[@shomusbiologyofficial]

+EKE IFEANYICHUKWU glad you liked it

[@smiritigupta5681]

Sir your videos are really very helpful in clearing my doubts and after watching your videos i dont go for any other online videos 👍

[@shomusbiologyofficial]

Nice to know that my lectures are helping you out

[@naveenkanthj9404]

Really usefull bro.... preparing xams by watching ur videos not reading books....

[@Aestheticalien.]

Thankyou so much for your efforts sir😊❤

[@shomusbiologyofficial]

You're welcome

[@stanzmahiii5819]

God bless uh....I was very unfamiliar with the topic ..u made it superb clear 👌👏🙌🥰🥰🥰

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@renadgalal14]

Thank you very very much i wish my school teacher explains like you

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@chelseawebster4028]

This video is a life saver- thank you!!

[@shomusbiologyofficial]

You're welcome

[@BrownieCraver865]

Thankyou so much! this video is so helpful for me cause I'm studying biotechnology 🙏

[@shomusbiologyofficial]

I am honoured

[@medhykhalil9169]

Thank you Sir you made me understand RIA in 9 minutes 🤝

[@awke5640]

ELISA ASSAY IS A GOOD METHOD FACILITATE TO KNOW OR IDENTIFIED DISEASE EASILY AND THAT DISAESE INCLUDING HORMONES,HBVSAG,RVI ETC. AND THE LECTURE WAS CUTE AND THE LECTURER WAS DEEPLY EXPLAINS THE ELISA,PROCEDURES,PRINCILES ETC.

[@shomusbiologyofficial]

+Abdisalan Awke thank you

[@gowthamim8986]

Thanks a lot sir!! 🤗 Ur videos helped me at last time of exam..... While I was struggling to understand and I'm scared what to do?? In exam..... I watched ur video and i did well in exms..... Thank you so much.... Really it's very helpful ✨

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@nopinkienoluyolo883]

I learn everything in your videos, they more informative as a new comer in a molecular studies I find it to be more interesting through your videos.

[@Neer333]

Wonderful depiction and explanation. Thanks

[@shomusbiologyofficial]

You're welcome

[@saniaambareen226]

Thank you so much for this video sir 🙏❤

[@shomusbiologyofficial]

You're welcome

[@Deepikaaryarsp]

Thank You so much sir... your lectures helps me a lot... 😃

[@shomusbiologyofficial]

Glad you liked my lectures

[@parikshitboricha5517]

Very helpful for understanding concepts

[@betulceylaner]

This is definitely so helpful, i can say that i understood it finally. Thank you very much

[@hangoo91]

Very good explanation thanks a lot

[@shomusbiologyofficial]

You're welcome

[@saminurr5793]

You saved me for attending viva 😁....Great thanks for your effort

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@vishalbandi9188]

Thanks sir it claarified my concept

[@shomusbiologyofficial]

Thank you

[@satvindersingh3644]

Thank you for explaining Mr Nanda 🙏🙏

[@shomusbiologyofficial]

You're welcome

[@anandparimal8812]

wow you made me understood in a single shot🙏🙏

[@shomusbiologyofficial]

You're welcome

[@hellobro2465]

One of the best presentation ❤

[@shomusbiologyofficial]

Thank you

[@uqwu2097]

Thank you very much, it's really helpful.

[@shomusbiologyofficial]

You're welcome

[@uqwu2097]

​@@shomusbiologyofficial I come from Indonesia, I continue my master's degree in KKU, Thailand and I have been watched your videos and really it's helpful for me, keep going on to create great content. thank so much.

[@wendwesengetahun6082]

Thank you,,my best teacher !!!

[@shomusbiologyofficial]

You're welcome

[@rachitsrivastava4958]

U are best in this field sir

[@shomusbiologyofficial]

Thank you

[@biotech451]

Your videos are just superb.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Yo-rz1qm]

Thank you so much sir it was so clear.... Great for your efforts... 😊

[@shomusbiologyofficial]

Thank you

[@rethijja3331]

This was so helpful thanks

[@shomusbiologyofficial]

You're welcome

[@shubhamb.deshmukh7920]

I think the substrate added after addition of Enzyme-linked antibodies is Chromogenic (which is itself colirless but forms coloured end product)

[@vikramjoshi3452]

Yes

[@chetan_thakur]

Yes

[@shrutibajaj2701]

Was looking for this comment 😛

[@mehnazmariyammainy6261]

Thank you for your good explanation......

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@aishaanwar6287]

thank you so much for explaining so clear

[@shomusbiologyofficial]

You're welcome

[@Ramachandran-io2eo]

Thanks sir. @@shomusbiologyofficial

[@zaara8151]

Bhai itna clear kaise samjha lete ho🙏🙏🙏. Thnk a lot

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@tiobstagashaw7100]

Thank you very much,you explained it the best way

[@aristeiasales7194]

Thank you. Very clear explanation :)

[@suvojyotinandi1648]

It's really helpfull sir. Thnk you😊🙏

[@shomusbiologyofficial]

You're welcome

[@habeebhussain2029]

That was amazing sir...my doubts were clear up..thank u soo much....

[@shomusbiologyofficial]

+Habeeb Hussain thank you. Glad you liked my lectures

[@razanfarah1879]

Thank you so much

[@shomusbiologyofficial]

You're welcome

[@nimrailyas7120]

Thankyou sir ✨ understood 🤟

[@shomusbiologyofficial]

You're welcome

[@marianohalescoscolla6962]

very usefull. Thanks a lot!

[@shomusbiologyofficial]

You're welcome

[@naureensharmi9262]

Your videos are so perfect! 😍

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@sameeraboh2050]

How good explanation!! Thanks

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@yogeeta-4477]

Best best best!! Thankyouu so much✨

[@shomusbiologyofficial]

You're welcome

[@bowbowvlog6697]

If u put eng subtitle is more good for hearing impairment ppl

[@dedicatedtorbc2588]

💜💜💜💜💜always helpful this channel

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Slowworld2.o]

very helpful for me as one day ago of my exam................thnkyu

[@zarnasolanki8522]

Thank you so much sir 😌

[@shomusbiologyofficial]

You're welcome

[@snehaar1814]

thank you these are very helpful for all my semisters

[@unmaskingdude9211]

Useful Actually.....Very Useful ♥️🙏

[@shomusbiologyofficial]

Thank you

[@josnajithmathew9946]

Thank you 💖

[@shomusbiologyofficial]

You're welcome

[@Sheezzz123]

You are such a heaven sent. Thank you for explaining things so well!

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@funnytakarar4105]

Thank you sir ... lectures helps me alot

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@gurleensaini9056]

Very good sir 👌

[@shomusbiologyofficial]

Thank you

[@arunscans9324]

very clear and nice explanation thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@gracejohane9063]

Amazing video,clear and understandable.

[@ajitdardare4565]

You are superrb bro.....this is fantastic video

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@rareandwondrous]

great explanation thank you!!

[@shomusbiologyofficial]

You're welcome

[@shinikrishna6982]

Thank you sir...❤️❤️❤️❤️❤️

[@shomusbiologyofficial]

You're welcome

[@shanzajamil8516]

once again You saved me thanku so much for the gret videos and superb explanation :)

[@shwetadubey1546]

Thanku very much 🙏👍

[@shomusbiologyofficial]

You're welcome

[@debrajdas4628]

Thank you sir.....

[@shomusbiologyofficial]

You're welcome

[@manasiovhal3134]

Thank you 👍

[@shomusbiologyofficial]

Thank you

[@lifelif2813]

Super💗👏👏 My exam is tomorrow and this is so helpful 🥲

[@shomusbiologyofficial]

You're welcome. All the best

[@ahmedeldesouky1878]

thank youuu so much

[@shomusbiologyofficial]

You're welcome

[@Adarsha__the_daily_diary]

Well explained

[@shomusbiologyofficial]

Thank you

[@aneesha1130]

Wow.... this is the best video.... 😲

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@manubashir554]

Very helpful 😊

[@shomusbiologyofficial]

Thank you

[@tehreemfatima9450]

Nice explainaction

[@shomusbiologyofficial]

Thank you

[@supravomandal515]

Awesome sir!! Thank u so much

[@shomusbiologyofficial]

You're welcome

[@pushparajsingh2553]

Good afternoon sir Thanking you sir

[@dedicatedtorbc2588]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@maheendhanani4212]

thank you! i have one question though, how do you know which antibody to add in the beginning though?

[@akashvani3836]

Actually ELISA is done for a specific test like AIDS DENGIE etc... so the antibody differs for different tests...for further queries you can ask...

[@kaneezfatima5144]

The specific antibody atificially prepared used to be there in labs. Say if we(doctor) ask a patient to get a HIV test his serum is put in that well which would be already having HIV antibody so if he is HIV positive his serum would be surely having antigen that would bind the antibody of that well....

[@kaneezfizza9484]

Thank U so much

[@shomusbiologyofficial]

You're welcome