This animation is enlightening. Cre-loxP system is indeed an intricate wonder.
@matteobarca10505 жыл бұрын
Thank you SO MUCH. Why in my university there are not videos like this one? GREAT JOB and what an incredible genomic tecnique!
@abmgood5 жыл бұрын
Hi Matteo, thanks for watching and leaving such a nice comment! Please let us know if you have any questions and we'd be glad to help!
@richard.f4 ай бұрын
Very helpful! Greetings from Brazil.
@abmgood4 ай бұрын
We're glad to hear that!
@marcossanchez75945 жыл бұрын
Extremely helpful video, THANKS!
@abmgood5 жыл бұрын
You're welcome. Thanks for watching!
@harryfire4113 жыл бұрын
Dr. Moreau approves this video
@vitorluis51622 жыл бұрын
Amazing video! Why is it called a FLEx switch if it's irreversible? You can't toggle it off after inversion, right?
@omoooooo4275 Жыл бұрын
Very useful! Thank you so much!
@AlexFower4 жыл бұрын
Great video and explanation, really helpful!
@abmgood4 жыл бұрын
Thanks for watching!
@CarolineBay4 жыл бұрын
such a helpful video! Thank you!
@abmgood4 жыл бұрын
Hello Caroline, Thanks for watching! We are really glad to hear that our video was helpful :)
@parastoomohebkhodaei39182 жыл бұрын
it was so helpful thanks a lot
@abmgood2 жыл бұрын
You are welcome! Glad it helped!
@amandarojas59905 жыл бұрын
How is it that these lox sites flank genes of interest without including other genes? It seems unlikely that so many genes are flanked by palindromic sequences, but am I incorrect in thinking that?
@abmgood5 жыл бұрын
These lox sites are typically inserted into expression vectors and genomes by researchers in very precise locations. They do not exist naturally as flanking sequences of particular genes. Researchers will determine where they want the inversions to occur, and will either clone in the lox sites into their plasmids, or perform knock-in experiments to insert the lox sequences in the desired positions in the genome before carrying out their Cre-induced inversions. Hope this helps!
@abcxyz98523 жыл бұрын
@@abmgood This is super helpful, thank you very much!
@Premark3 ай бұрын
Really short n sweet
@abmgood3 ай бұрын
tysm!!
@mayukhroy53934 жыл бұрын
This video was very helpful.
@abmgood4 жыл бұрын
Hi Mayukh, thanks for watching our video! Please let us know if you have any questions and we'd be glad to help!
@muhammadhisyamfadhil8252 жыл бұрын
Now I can continue my article.. thanks!
@abmgood2 жыл бұрын
You're welcome ;) Thanks for watching!
@edilsonjuniorpsi2 жыл бұрын
Cre é projetada para um tipo de célula em específico? É preciso encontrar um promotor específico de uma determinada célula que deeja a marcação e então produzir uma cre recombinante própria para ser ativada por esse promotr em específico?
@sumitrasaini21584 жыл бұрын
It helps to clear my basics
@abmgood4 жыл бұрын
Hi Sumitra, thanks for watching and glad our video was helpful - please let us know if you have any questions and we'd be glad to help!
@peterlauridsen84033 жыл бұрын
very informative!
@abmgood3 жыл бұрын
Thanks for watching! :)
@chantal_zz_ Жыл бұрын
Thank you
@jeromekoestel12584 жыл бұрын
amazing explication!
@abmgood4 жыл бұрын
Thank you! :)
@Donniejoe12342 жыл бұрын
Thank you! :)
@abmgood2 жыл бұрын
You're welcome!
@swetakushwaha90494 жыл бұрын
Nicely explained!! I have a question..how to incorporate cre sequence in a mouse at the gene specific promoter? And also the lox p sites at the specific position?
@abmgood4 жыл бұрын
Depending on whether you would like to overexpress the cre sequence using a gene specific promoter, or to knock-in the cre sequence under the endogenous promoter, gene overexpression constructs or CRISPR knock-in can be used. If you have a specific project in mind, please contact technical@abmgood.com for further discussion.
@thefenerbahcesk41565 жыл бұрын
Why does it matter if a gene is inverted?
@abmgood5 жыл бұрын
The gene must be inverted in the OFF (no expression) condition for the FLEx system. The Cre-Lox FLEx system controls expression by changing the orientation of the gene in relation to its intended promoter. For example, in the OFF position, the promoter would be in the forward direction while the gene (flanked by Lox sites) would be in the reverse direction, which would not lead to expression of the functional gene. After Cre is introduced to turn the system ON, the gene would be flipped into the forward direction and be able to be expressed under the control of the promoter. Hope this helps!
@redbluelocke42692 жыл бұрын
I know I have the genes to be sexy. I just need the switches to be flipped