Cre lox system | Conditional gene knockout using the Cre Lox system | site specific recombination

[@IlyaKuzovkin]

Thank you for making this video! It is by far the best one I have found on the topic of what Cre Lox system is and how it works.

[@animatedbiologywitharpan]

please share my channel link with your friends

[@priyankanehrwal1935]

I was very confused about using TAM for activating cre. You made it very clear. Thanks a lot.

[@animatedbiologywitharpan]

youtube.com/@letscrackbioexams94?si=ZWdPXv2NSpZeDHZu please subscribe to my second channel 🙏

[@aj7612]

Your visual and simple explanations are so amazing! Thank you for your channel.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@IlyaKuzovkin]

Thanks!

[@animatedbiologywitharpan]

Recived with thanks. Please suggest topics on which you want to see videos

[@irisfan5386]

The best explained of the system I have heard, thank you

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@kngoud4647]

Wow , one of the best video on cre lox p . Thank you so much ... I understood 😊😊 #from UoH

[@animatedbiologywitharpan]

Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

[@puekai]

Why is this technique not given a Nobel yet?

[@animatedbiologywitharpan]

No idea

[@Worldstop-bw9pn]

nobel prize is no longer for true scientist, it is for corporate scientists that's why

[@semb12]

Thanks for this video. Best explaination I have found.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@HaimingWang-c1w]

Super well-explained video! I hope you are my professor teaching me!

[@animatedbiologywitharpan]

Please support me by subscribing my second channel youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve

[@puekai]

The explanation is quite clear

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@nilaratnadas5246]

Thanks for explaining the application. 🙏🙏

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance.

[@AliviaMiriani]

Thank you for being so clear in your explanation, super helpful video. I have a question; how can we use the CreloxP system to restrict a particular gene knockout to a certain stage of development (i.e. adolescence)?

[@animatedbiologywitharpan]

You need to use the cre ER system . Tamoxifen inducible Cre-estrogen receptor (ER) fusion protein. For this, Cre is fused with the ligand-binding domain of a mutated ligand-binding domain of ER, which does not bind to estrogen but binds to tamoxifen with high affinity. Cre-ER proteins are sequestered in the cytoplasm via association with the HSP90 chaperone. Upon addition of tamoxifen, tamoxifen-bound Cre-ER dissociates from HSP90, translocates into the nucleus, and carries out site-specific recombination between flanking

[@AliviaMiriani]

@@animatedbiologywitharpan Thank you for your help!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@gabrielamaral3167]

Thank you my friend, great explanation!!

[@berlyglobe7]

Can you also discuss how does Off-target recombination can occur with Cre? I think its also called Cre leakiness... I'm still confused

[@animatedbiologywitharpan]

OK I will make a video on that

[@shruthi8974]

clear explanation thank you

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@VincentZhao-pb2hs]

so good thank you

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@elsieureta2719]

Thank you for making this very simple! Very helpful!

[@animatedbiologywitharpan]

please share my channel link with your friends and help me to reach big audiance

[@nishat_zaman]

Amazing video!! I have a question, so in the LoxP mouse, all the tissues will have the gene you want to delete flanked by LoxP sites. And only in tissues where Cre recombinase is active will you have the gene deletion occurring. Do the LoxP sites around the gene interfere with the correct transcription/translation of the gene in tissues where Cre recombinase is not expressed? I am wondering whether the LoxP sites could interfere with the translation of the gene.

[@animatedbiologywitharpan]

Good question, in most of the cases it does not interfere. The loxP sites are small.generally they flank exon and not in orf.

[@kriteebiswas488]

Hi, can the cre recombinase travel to other tissues (even though it has a tissue-specific promoter) and knock out the GOI e.g. the cre is brain-specific but can it travel to the liver and knockout loxp flanked gene of interest? And is the loxp flanking also tissue-specific?

[@animatedbiologywitharpan]

No it can't

[@barbaramoreno329]

In KPPC models what does it mean when they say there's Kras recombination or P53 recombination?

[@animatedbiologywitharpan]

I am not sure about this

[@ibrahimhassoun2595]

Thank you man!

[@animatedbiologywitharpan]

Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.

[@PinkuHalderVlog]

very nice explanation

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@marcom0196]

Incredible video !!! Thank u very much for this clear explanation !! ❤

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@rheaseth]

Thank you for the nice video. Could you please explain once how the LoxP mouse would be generated?

[@animatedbiologywitharpan]

yes i would make a video on that

[@ManuelGomezLopez-wf5is]

nice video

[@animatedbiologywitharpan]

@@ManuelGomezLopez-wf5is Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@codycasanova2579]

Why the phosphodiester bond on the 2' carbon

[@yuribanicol9490]

hola, podrías ayudarme, como resolverias este problema: “Usted generó un ratón KO para el gen XYZ. Luego de la obtención de las quimeras usted obtuvo ratones XYZ+/-, los ratones XYZ+/- son viables. Al criarlos se obtuvieron crias con la siguiente frecuencia: 50% XYZ+/+, 25% XYZ+/- y 25% XYZ-/-, pero los ratones KO mueren a los pocos días de nacer. Usted decidió hacer un knock in para el gen XYZ, con el fin de verificar los tejidos en los cuales se expresa, encontrando que se expresa en el hígado, páncreas y riñón. ¿Cuáles son los pasos necesarios para obtener un ratón donde el gen XYZ solo se elimine del páncreas? Esquematizando los vectores que se utilizarán, indicando todos los elementos importantes para la transgenesis.”

[@tushyamehta]

Can you explain how AAV might be used with CRE-loxP sites

[@animatedbiologywitharpan]

I will try to make a video on that

[@Lulla3sl]

thank you! better than me prof definitely lol

[@animatedbiologywitharpan]

Please support out channel using super thanks. Your 1$ contribution is our motivation

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@akemumoren4133]

break it down for me.. so cre is what, a virus? and when does one use cre-lox or creER-lox? thanks

[@animatedbiologywitharpan]

Cre is an enzyme ( it's a recombinase enzyme). Cre loxp system can be used to knockout genes.

[@anshuu5339]

E knockout rat killer cake 3, 4 packs thinty chachipotara emaina effect untada leda chepandi

[@animatedbiologywitharpan]

I have no clue

[@pranabkarmakar7775]

Please make it a detailed version of this video! You have a great concept on these topic but when you are teaching you are jumping into related topics very often thats makes understanding quite difficult! Please make a detailed video! I request you! Thank you sir!

[@animatedbiologywitharpan]

Thanks for the detailed feedback

[@anshuu5339]

If one person can eat this knockout rat killer cake 3, 4 , packs what will happens

[@animatedbiologywitharpan]

I haveno clue