Decorating My Tree With Bioluminescence

I love the holiday season but have never gotten to decorate a tree, so this year I thought I'd change that. I picked up a beautiful pine tree, and set about decorating it. First I genetically modified some E. coli to fluoresce under black light in a variety of colors. Then picked up some bioluminescent marine algae. In this video I go through how I used these different organisms to make some stunning decorations and decorate my tree. And while I decorate, I took some time to look back at some of my favorite projects from 2019 and talk a bit about all the exciting ones coming up in 2020!
Taste Emporium: / thetasteemporium
Algae: pyrofarms.com/
Videos I mentioned:
Cloud chamber - • Make Invisible Radiati...
Spider Silk Overview - • Spider Beer - Making Y...
Glowing yeast - • Engineering Yeast to G...
Eggs Benedict - • Eggs Benedict Don, Fit...
Ultrasonic Alcohol - • Instantly Age Alcohol ...
They sent me water instead of DNA - • They Sent Me Plain Wat...
Neuron - • Growing Human Neurons ...
Meat berry - • A Grape Made of... Mea...
Cold Fire - • Cold Fire You Can Touc...
Glowstick of Doom - • The Terrifying Glow of...
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Ko-Fi: ko-fi.com/thoughtemporium
Become a member: / @thethoughtemporium
Store: thethoughtemporium.ca/
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Website: thethoughtemporium.com/
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[@Halesnaxlors]

Nothing says Christmas like enslaved glowing poop germs. I love it.

[@ayupermata1367]

I love that

[@Gabriel2.0]

of course you have e.coli in your christmas tree...

[@MrN1c3Guy100]

better then having it in your food. ;P

[@swedneck]

@@MrN1c3Guy100 i'm pretty sure E. Coli is basically on every surface

[@synchrolord]

Well uf he got sick he could probubly cure himself

[@invertacreator5865]

E. coli are pretty common and only some or half or so harm you it’s more like a body shape of bacteria

[@pandamaster8306]

He said it so calmly too

[@darrennixon8765]

‘My friend sent me some DNA’ I think we all know what that means

[@Mr3344555]

Oh you Nixon's! 😂

[@cryingwater]

Maybe he meant his sugar daddy

[@Ezekiel_Allium]

@Ryan Rafter imagine having the kind of friend where you can say "oh boy hope theres cum in my mailbox today!" Lol

[@ladystarlightasmr]

You could literally just do a cheek swab

[@science_bear]

@@ladystarlightasmr yeah but that’s not as fun

[@ThePhantazmya]

"What did you do for Christmas?" "I decorated my tree with E.coli and Algae." Happy New Year!

[@TehErectedSponge]

Can't wait to see him telling the robot "You pass butter".

[@MrGoatflakes]

And then the robot misunderstands and does a crap of butter onto a plate for him?

[@a2pabmb2]

@@MrGoatflakes I'd buy that for a nickel.

[@debbiehargreaves3350]

Oh... oh god

[@serenitytrek]

Meanwhile: disposal pile escapees creep foreward singing "This Little Light of Mine"...👾👾👾...🌆🌌

[@DerSolinski]

Next year: GMO Glowing Pine tree... "Save on decoration and electricity"

[@DejcoDoesStuff]

That glows only when disturbed.

[@DerSolinski]

@@DejcoDoesStuff Uhhhhh Imagine a tree nursery when the wind whistles through. That would be a sight to behold. Yup can we do that please, I want that!

[@JM-us3fr]

Or engineer it so it only glows when given a certain time of water to drink.

[@DejcoDoesStuff]

That would really be a site to behold.

[@paulsmith-gi5vm]

Your tree decorations are fabulous and educational. I hope they inspire others to understand how the sciences bring light and joy into one's life.

[@timschafer2536]

I have to say that this channel is by far the most amazing one I’ve subscribed. It’s science but not just theory it’s practical and with little to no equipment. Then it is also diverse in its topics. I originally subscribed because of the radio projects and the satellite image project, but the biological stuff is fascinating. I’m particularly interested in how the brain computer interface Project will turn out. I wish y’all a happy new year.

[@JamesDoesStuff0603]

Chrysippus styro is god

[@lipo3502]

I'm very impressed by all the projects you've invested yourself into. I respect the amount of work and efforts you put in your videos and it is very inspiring. Keep up the great work

[@herlofrumfragi4361]

Forcing bacteria to light up my Christmas tree

[@MrRolnicek]

How do you just ... "design a completely new type of silk that doesn't exist in nature"?

[@thethoughtemporium]

well I did it at like 2am so the details are a bit fuzzy.... Kidding of course. I mean, I did do it at 2am, but was done carefully and I kept detailed notes. The long and the short of it is that I found 6 short pieces of silk DNA from various types of silk and spider species, and then carefully randomized their codon usage and ordering to allow the DNA to be successfully printed.

[@Nae_Ayy]

@@thethoughtemporium I am so happy to be alive in the time we are now. Science is fucking awesome. "I'm having trouble extracting spider silk DNA, so maybe I should *fuckin design my own.* "

[@MeepMu]

@@thethoughtemporium How is DNA printed?

[@seppe1138]

@@MeepMu with a dna printer

[@deadgaming20]

@@seppe1138 lmao

[@KittyBoom360]

Netflix's "Lost in Space" also used bioluminescence to decorate a Christmas tree in their second season just released this month.

[@Kuratius]

2120: So we gave making cat girls a try

[@JamesDoesStuff0603]

Kuratius me in 3 years

[@abdito_ry]

its actually possible to do that we just don't have money to try it

[@bowlofpasta8846]

@@tigertoxins584 regardless of if it's possible or not it's just not ethical or worth it. The joke is so overplayed and overdone because some weaboos don't want to admit they're furries

[@niko2239]

@@bowlofpasta8846 we could also grow tentacles out of our bodies, so that's an option lol

[@ELbabotas1]

Yeah, also, you'll have to raise it

[@CJ_McK]

This is quickly becoming one of my favourite KZbin channels. Thanks for sharing all your hard work with us.

[@zetareticuli757]

For next year's tree, I suggest looking into lichen. Some strains have amazing fluorescence.

[@JamesDoesStuff0603]

My dad first showed me your brain cheese video and since then I’ve watched all your videos I’ve wanted to be a biogenetic engineer and I’ve learnt so much during watching your videos. Your channel has also inspired me to try to start a genetic engineering club at my school! I love your videos keep it up!

[@_ccyberia]

No one: The Thought Imperium: *Hey my friend send me DNA*

[@BLUYES422]

just wanted to say that i love this channel. i'm ALWAYS excited when i see theres a new thought emporium video. super excited to see whats up with that synthetic spider silk. im a programmer and im super interested in the plasmids make up

[@RobMellor]

I never cease to be amazed at this channel. i just don't see how you have the endless inspiration, dedication and ability to keep churning out these absolutely fantastic projects. Merry Christmas

[@serenitytrek]

You had me at "bioluminescent"...👾❤

[@revenevan11]

Your channel and how you show the fact that this stuff is possible diy, is the main thing that makes me feel like I'm actually living in *[current year]*

[@engineer0239]

Your whole channel... It's just... Amazing. You are the best Science Channel on KZbin 2019. Congrats. I'm confident that 2020 will be even better. Keep it up!

[@JacquesDV100]

I'm glad I found this channel, you make some awesome stuff man. Looking forward to all the crazy stuff next year. Thanks, good luck and happy holidays and new year

[@morkovija]

I cant believe this is possible for a non-darpa level of funding. Unbelievable. Cant wait!

[@Draakdarkmaster6]

couldn't hit that subscribe button hard enough for your second channel, glad you had good holidays and finally got to decorate your own tree, hope you have a happy new year as well.

[@solidacid1337]

I REALLY enjoy these videos! every single video so far has been AMAZING. I especially enjoyed the wifi radiotelescope videos

[@drummerkingrulez672]

So long as I live that reference will ALWAYS MAKE SENSE!

[@noboto4686]

I very rarely comment. But your channel is too great not to say something. Honestly, it's up there in my subscriptions, truly amazing content. So thank you. Can't wait to see what's coming up in 2020!

[@sohaib303]

Looking forward to your new videos, and being a neurosurgeon, especially the neurons on chip project. Your channel has inspired me alot, with bold new ideas.

[@Axileoni]

You make some of the best videos on KZbin. Never change.

[@lrvfb]

Honestly this is the best channel I´ve ever come across on youtube.

[@snootdingo9365]

It's so cool to see a man of great intellect take part in our winter revelry. We hope you enjoyed our humble celebrations.

[@AscendtionArc]

Thanks for the cool video and massive props to you for using your tree.

[@vishnukolal5208]

hey man, i think yours is one of the most underrated channels on youtube. i LOVE your videos am excited to see more.. happy holidays and all the best for all your future endeavors!

[@garethronaldo8692]

good job and i am looking forward for your new projects 👍

[@Suchega_Uber]

So glad you pointed out that pine is a good flavor. I used to grab needles to chew on while we walked around town as a teen. My friends thought I was nuts, but eventually I got them to try it, and they were really surprised. If by chance anyone has any good recipes with pine, let me know.

[@Davvg]

A beautiful and E. Coli scented Christmas tree

[@CookieCraftMedia]

This channel and the videos you make are so interesting, keep it up!

[@BlueTJLP]

Absolutely fucking brilliant again. Your videos are more or less hidden gems on KZbin. I never regret clicking the bell for your channel.

[@jacewalton6677]

I made my Christmas tree glow with poop germs that I genetically modified in my shop.... Me: I'm listening....

[@hugoreze]

Gsus... man, you bring the DIY to an insane level....

[@JamUsagi]

So looking forward to the Taste Emporium

[@MrThonny15]

I love this shit so much. Looking forward to your 2020 videos!

[@MrKclo42112]

thats totaly something I would have done ,awesome Christmas tree,

[@Rotem_S]

Just being able to buy finished genes for gene editing in the mail for less than the price of a laptop is amazing and I really need to try it sometime

[@revenevan11]

Even though it's almost new year, do you finally feel like it's 2019 after finding out about that? 🧪😁

[@franciscojavierramirezaren4722]

Happy new year!! my best wishes!! greetings from Mexico☺

[@wlritchi]

This might be the greatest hype video the world has ever seen. I can't wait!

[@cocok.291]

When the apocalypse hits you're gonna help humanity rebuild

[@BothHands1]

every video is just amazing

[@user-rc4zk8ge1g]

I both appreciate and hate this channel for showing science for what it is: a process of iterating failure and frustration, sometimes with a satisfying end result.

[@sagal.h.462]

You are probably the coolest guy ever! Happy new year! PS. I love your neuron- electron project! I wish i could grow my on brain like that and put it in a robot body and somehow transfer my consciousness into it.

[@ftrouve7180]

Have an awesome holiday season!!!

[@Narnald_Ewman]

Super awesome!

[@samhoff1724]

I know it’s gonna be a good day when you upload

[@jasondworkin6597]

PyroDinos look amazing. I failed to do a project involving isolating dinoflagellate luciferase in 1990 as there was just no commercial source of bioluminescent dinoflagellates at the time.

[@The_Mimewar]

Same. I wasn’t raised with Christmas as a Jesus thing, and I am not religious at all, but as an adult, I have kids, and I LOVE Christmas. It’s such a fantastic time of celebration and optimism!

[@AjinkyaMahajan]

You always amuse me.

[@acompletelynormalhuman6392]

I love your videos so much and half of the science you do berlines on Magic

[@EverythingDigital5779]

This is by far the bast channel on KZbin

[@namless4508]

Amazing video. I hope you will have more cool videos in 2020

[@Zen-rl5pv]

Wish I could subscribe twice, love all your content and can't wait to start producing some just as good :^)

[@loonyt22]

Would love to see something from you about the attempts to make an open source insulin. I know there is a group working on it.

[@gdn6664]

Very cool!!!

[@GoldSrc_]

This grants you at least 1 million nerd points, amazing. Oh boy, 2020 will be lots of fun.

[@vidal9747]

It is nice to see that you, apparently, love various fields of sciense.

[@haydenparsons5783]

This dude just does things people dream to do and go to school and train for years for fun

[@V1ctoria00]

The main issue with generating light from sound, "sonoluminescence" is that it requires very sensitive frequency modulation of a specific area. The resonant frequency of the liquid you wish to suspend a bubble in must be matched, and then maintained. Then once the bubble is captured the device can be tuned to apply pressure to the bubble. If someone coughs on the outside surface of the container of liquid it can be enough to pop the bubble. Also. Temperature is a non considered variable so far. Both the proper operating temperature of the entire device and the active temperature fluctuations from within the bubble. Apparently the gas inside becomes plasma when squeezed properly on all sides. Plasma In a bubble trapped in resonant frequency matched liquid is Supposedly in excess of 10,000⁰F. The bubble will bounce from this tight plasma state back to "full expansion" and shift from that extreme high temp state to under -250⁰ extreme low state and oscillate between the two until disturbed. So the second issue is how to deal with the super cold temp, and harvest the light, electricity, and heat coming off the plasma.

[@Ferroes]

Thought emporium after growing innumerable failed artificial meat: *I’ve decided to open a cooking channel*

[@larchaspen6818]

Merry Christmas

[@HeaanLasai]

Amazing!

[@Funlifedoga]

Damn , that is cool!

[@DrEru]

Black agar and gloves. Cool.

[@lablulz2483]

incredible stuff as always, please never stop posting!! looking at the balls, I wonder if agar would gel while in constant motion? you might be able to use a rock tumbler setup to do a roto-gel and evenly coat the inside. Heads-up about christmas trees: They have a reputation for being heavily sprayed with pesticides. Personally, I collect them this time for the next year's worth of bonfires :)

[@MenkoDany]

Alright, you earned my subsr- oh, I'm already subscribed Damn, you're good!

[@andrewhamop6665]

Yay new video!!

[@albertogregory9678]

Oh this gonna be good

[@kareno8634]

You have GREAT Friends! *8 ) CHEERS!*

[@skop6321]

stop reminding me of your cloud chamber . . . you're making me jealous. I want one.

[@fuyuminekimaya7571]

your videos are basically my study (applied science) but then fun xD

[@Saka_Mulia]

Please upload the previous food video you did to the Taste Emporium channel for those of us too lazy to search your channel. Also, thanks for the informative videos you make, can't wait for another years worth of them :)

[@thethoughtemporium]

It's linked in the description already

[@BracketsAttackets]

Holy mother of _Mortasheen city itself_ ... That's one way for a biologist to party!

[@unofficialaudiobooks7878]

Yay another video

[@bjarnes.4423]

Nice, more genetics!

[@travpots6318]

Good job

[@matts2581]

Tree of Life! :D

[@foxytv1592]

Dude I literally saw shokugeki no soma right till season 4 episode 11 because of you. That show is kind of too foodgasmy but those dishes are crazy!

[@Lillly353]

Really Got an ad for cat food with algee😂

[@Steve-uu7yx]

this is sick!

[@OccamsPlasmaGun]

I held back for 30 comments, hoping that someone else would be "that guy". That's not a pine tree. It is a spruce. ;) Love your videos.

[@pattheplanter]

Very clearly a spruce and not a pine or fir. I am sure he did a close-up of the branches to test us. He will probably reward you with some spruce beer. Nobody makes pine drinks, do they? Pine tastes like floor cleaner, spruce has a much nicer flavour. Also contains vitamin C in the young shoots.

[@CJ-df7gf]

It's all fun and games till the cat knocks down the Christmas tree

[@israelramos7441]

YOUR CHANNEL IS AWESOME!! 🤣🤣🤣

[@fcknhllkllyll3767]

Im so damn exited for the next braincheese episode!!

[@voidbeing7754]

You are my hero

[@Nyarmith]

local man unleashes e-coli on the jolliest day of the year

[@vidal9747]

The year is 2019 and you can create and order DNA for $800. It seems like sci-fy to me.

[@3rdHalf1]

I can't wait to get hobbyist 3d printer equivalent of DNA editing tools. How awesome would it be to re-create some kind of super-virus, based on leaked documents from some university. :D

[@vidal9747]

@@3rdHalf1 FBI, Open the door! hahaha

[@ELbabotas1]

xD, we all joke about a real possibility... What the heck would you do with a ailurantrope? Drive it to kindergarden? Make sure it has all its shots with the vet? Go get groceries with it on a leash like a football mom with a HDD human larvae? These critters would be alive, y'know? What in the freaking heck does one do with a catgirl?

[@ClockworkAvatar]

wish i could like more than once.

[@sealpiercing8476]

I keep managing to forget that these days you can order custom DNA for similar price as CNC machining a custom mechanical part.

[@kar27k]

1:43 "... I realize in another decade that reference in isn't going to make any sense" I think it's already doesn't make much sense, as CD drives are pretty much absent.

[@CrazyGamer1541]

From PCs. Most laptops still have disk drives

[@ThePlayfulJoker]

Sorry to point this out but I think you mentioned "the new decade" right at the end of the video, which is not the case as the new decade starts only in 2021. Anyway, happy new year! Love your videos so much!

[@Volvary]

Ah yes, the cooking channel is now a thing. Now all we need is to get you and Nigel to collab on episodes using some of his edible chem into the recipes you guys make.