Determination of Starch Content | A Complete Procedure | Acid Hydrolysis Method

Starch constitutes the major component of the polysaccharides in most foods and consists mainly of amylose and amylopectin. The test sample is dispersed in water and heated in acidic solution to hydrolyse the starch, releasing the sugars. The resulting sugars are then determined by titration with Fehling solution according to the method of Lane-Eynon. The sugar content is further converted into Starch content of that sample. This video represents a detailed procedure for the determination of total starch content of a food sample by Acid Hydrolysis Method.
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[@JoshiNirav30]

Appreciate the amount of work and editing on this video, please make a video on "Total Dietary Fibre by Enzymatric Gravimetric Method"

[@MicroChemsExperiments]

Noted. Thanks

[@hannagraceguirigay1720]

Can you do a video explaining the formula used? Where does the 0.9 come from? 10:09

[@yadavhealthcom]

Thanks 😊

[@MicroChemsExperiments]

Welcome

[@vladimirosvasileiadis7742]

Hello i really need your help. I want to make an acid hydrolysis of proteins. Can you please tell me a method to do this? I know the most common is with hydrochloric acid but i want to learn the method. Thanks

[@MicroChemsExperiments]

Digest 2g of your sample with 20ml concentrated Sulfuric Acid

[@FST307]

share vedion on colouring matter determination in ready yo eat food

[@MicroChemsExperiments]

Noted

[@adepojulanre5234]

Why phenolphthalein indicator not added?

[@alyciaani]

what gas would be released during the acid hydrolysis please

[@nurulnadhirah64]

I want to ask, for the solution that needs to be tested for the total sugar method, is it the solution needs to do it immediately, or can wait for a few days?

[@nonatarbah2951]

Could you answer me How do you make acides? how calculate them? Is there a way or low for calculate base and acid which use in determine at lab?

[@MicroChemsExperiments]

Please study chemistry more to do these. Take help from your school teachers.

[@akanmudamilare7235]

Can this method be used for starch content evaluation in flours?

[@MicroChemsExperiments]

Yes

[@therese6051]

Hello! What specific type of reflux condenser is used in this process? Can we use the liebig type of reflux condenser? Or does it have to be the same design and type?

[@MicroChemsExperiments]

Any reflux condenser what will facilitate the condensation is applicable

[@nurulnadhirah64]

so to get the starch content, just only times with 0.9? why 0.9? may i know

[@MicroChemsExperiments]

The reference method said so.

[@narendarpratapyadav9908]

@@MicroChemsExperiments .92

[@nurulnadhirah64]

May i know what references do you use to get 0.9?

[@byrnardsalavedra5969]

hi sir/maam, can we use any type of condenser for this analysis? what is most preferred?

[@nurulnadhirah64]

do you have the method to determine starch content by using lane and eynon method?

[@nainanizami7924]

can we use this method for determining starch percentage in turmeric?

[@MicroChemsExperiments]

Yes. You can.

[@nainanizami7924]

is this procedure taken from AOAC ?

[@MicroChemsExperiments]

No. It is laboratory validated method based on Lane Eynon method

[@narendarpratapyadav9908]

sir starch industry ke bhi video banaye

[@MicroChemsExperiments]

Noted

[@ramisfadhil7384]

Total sugar 47.5 % how it come? And 0.9 😢

[@danielpecori6532]

Hello, can I use this method for determining starch porcentage in corn grains?

[@MicroChemsExperiments]

Yes

[@nonatarbah2951]

What the meaning 50% Naoh

[@MicroChemsExperiments]

Please study chemistry more to do these. Take help from your school teachers.