Determination of Total Sugar_A Complete Procedure (Lane-Eynon Method)

Total Sugar include sugars naturally present in many nutritious foods and beverages, such as sugar in milk and fruits as well as any added sugars that may be present in the product. Total sugar means the total amount of reducing and non-reducing sugar. This video represents a detailed procedure for the determination of total sugar content of a sample. __________________________________________________________________________________________
Follow us on Social Networks to Stay Connected:
KZbin: / @microchemsexperiments
Facebook Page: pg/MicroChem...
Be with us, be a Scientist. ______________________________________________________________________________________________
Music Credit:
Sound Cloud: / special-days-jayjen-au...
KZbin Audio Library: • Roadtrip - Scandinavia... __________________________________________________________________________________________
Related Tag:
#totalsugar
#sugar
#sugarcontent
#sugarpercent
#laneeynonmethod

[@Wardi_lovers]

I can't explain how much I'm Happy 😊☺️

[@MicroChemsExperiments]

Thank you.

[@sabshahchannel9530]

Hi. Nice video. I follow your method. But my sample titration reading is higher than blank titration reading. How to solve this issue? If we subtract blank and sample tiration volume, it will become negative.

[@user-gf7ji7mp5b]

Good , thank you!

[@MicroChemsExperiments]

Most welcome

[@Wardi_lovers]

Thanks for this video ☺️

[@MicroChemsExperiments]

It's our pleasure. You requested a lot for this video.

[@albaen3664]

I observed this method. i dont unterstand WHY during the sample titration you used the working standart solution as titrand used before IN BLANK TITRATION . In the method Lane Eynon THE TITRATION OF THE SAMPLE SOLUTION MUST BE DONE WITH THE SAMPLE SOLUTION AS TITRANT SO YOU HAVE USED THE WORKING STANDART SOLUTION(2.5MG/ML) TWICE. IS THAT CORRECT?

[@sonandpets1215]

Thank you! Your nice voice is more clear without the loud music. Would it be a lot to ask not to play the music? Your videos are amazing, clear, descriptive and super helpful! You're very much appreciated!!

[@MicroChemsExperiments]

Thank you. Stay with us

[@Wardi_lovers]

Very useful video 👍

[@MicroChemsExperiments]

Thank you

[@fannycastillo8558]

Hello. I hope you can answer me. I am developing a method to help me analyze the total sugars in molasses to implement it in the quality control laboratory of a company that produces Rum. I am a practicing student so that would be my contribution that I would leave to the company, I have looked for many methods but it must be a method that does not involve many reagents since I must work with the reagents they already have in the laboratory. Furthermore, being a company, its analyzes must be, so to speak, quick to carry out. This Lane-Eynon method seems appropriate to me because it is the first experiment I have seen that does not use many reagents and is quite simple to perform. If you have any suggestions on how to determine the total sugars in molasses, please I would appreciate your response.

[@princyagarwal2057]

Hello, Thanks for such a detailed and understanding video... Great work... This helped a lot.. It would be very very helpful if you could please share the official reference for this procedure along with calculation, as I have to include this in my thesis...

[@MicroChemsExperiments]

It is an in-house method.

[@rasmuspallisgaard1102]

Hey. Just wondering in the calculation of "S", how do you get (34,1-26,1)=8,7mL? Isn't it just 8,0mL?

[@MicroChemsExperiments]

Hey, you are correct. We did mistake in the subtraction, though it is a silly mistake. We are sorry for our mistake. And thanks to you for the observation.

[@AbishekStephen-pj3gt]

Hello., i tried your method and the color does not chnage nack to orange red after addition of 1% methylene blue. The blue color would not go away even after adding more than 26ml for working standard titration. Not sure what the issue is. Also, could you let me know your email to contact you directly

[@mohitsharma.9509]

Thankyou so much

[@MicroChemsExperiments]

You are most welcome

[@goda2002]

very awesome video and useful, thank you for this work

[@MicroChemsExperiments]

You are welcome

[@Guard_Jaron]

Hey there! I have a question: should it be possible to do this determination on a sirop which contains invert sugar (which is a mix between the same amount of glucose & fructose molecules)? The amount of invert sugar in the flask is unknown obviously, that's why I would want to do this experiment :). I do not know what the expected sugar concentration is atm, but I could calculate it because I already did a polarimetry on it. The sirop also has a light-brown color, if that even matters. Anyway, any help would be hugely appreciated!

[@MicroChemsExperiments]

Both glucose & fructose will be determined as together in total sugar by this method

[@bioquestsolutions]

8k

[@manuelgranadoscollazos5910]

That process is easier and should not take more than 20 minutes to complete if you have previously prepared the needed reactants.

[@mariafernandacarrillo6442]

Hi, excellent video , where can I read the procedure? Thanks

[@monicaaponte5333]

Does the stock solution necessarly have to be in repose for 3 days? or Can we heat it up to make the hydrolysis faster? How can we accelerate the reaction without using enzymes? :) thank you!

[@MicroChemsExperiments]

No idea. We just know to keep for 3 days according to Lane Eynon method

[@SIMARPREETKAUR-zy5uu]

Isnt this method for reducing sugars? Because for totals sugars we have to add citric acid to the sample prepared?

[@asmaalnuaimi4364]

Thank you for your video , I have question what do you mean by the concentration of original sample 60% when you add 5-7ml of the honey sample , i didn’t get it

[@MicroChemsExperiments]

Expected sugar concentration is 60%. That's why we added 5-7ml sample. Because sample amount varry depending on the expected sugar concentration in your sample

[@abdhjr4780]

Hello, is it the same procedure for dextrose equivalent determination?? Or you have another procedure because i need to test the dextrose equivalent for invert sugar? Please advise

[@MicroChemsExperiments]

This is dextrose equivalent test. You can use this.

[@aanchalgahalot8183]

Super. Thank you very much.do you have the videos estimation of reducing and non reducing sugar.if yes, please send. Thanks again.

[@MicroChemsExperiments]

Will upload soon

[@LabAnaysis1385]

Super

[@MicroChemsExperiments]

Thanks a bunch

[@ethiopia11]

thank you for this detailed video..., the first thing to ask on preparing the heating plate, at what temperature the pre-heated plate should be maintained also can we use this method for identifying total sugar in wheat or rice straws?...if any recommendations, and also you used sucrose in the stock solution preparation which is non-reducing sugar why is that since we are determining a reducing sugar ?... thank you

[@MicroChemsExperiments]

1. No specific temperature for hot plate. Use the temperature enough to boil the solution. 2. You can use this method for food sample only. 3. Sucrose is converted into reducing sugar while kept for 3 days in HCl.

[@hirunizoysa2977]

Thank you for the informative video . Can I use this method to determine starch content ?

[@MicroChemsExperiments]

Starch determination method will be uploaded shortly. Stay with us

[@anishaaroskar9936]

Why standard sucrose solution after adding HCL and water is kept for 3 days incubation ? Is it for Hydrolysis purpose?

[@MicroChemsExperiments]

Yes. For hydrolysis

[@maduabuchijohn7072]

Thanks for this video. Please if you say that this procedure is the same for estimating the dextrose equivalent, then why did you say it's the estimation of both reducing and non-reducing sugar? Dextrose is reducing only.

[@MicroChemsExperiments]

This method determine the total sugar, not only dextrose.

[@yeshkumar1796]

Hi,Nice vedio, I don't understand why we make 2.5gm working standard solution??pls explain

[@theresebalaga9514]

Hello, can I ask? What is your process on drying your sucrose? How did you dry your sucrose?

[@MicroChemsExperiments]

Keep the sucrose at 110 degree Celsius in hot air oven for 2 hours

[@conocimientofree2779]

hello thanks ! thats amazing ! one question ,Is this method suitable for solid foods?

[@MicroChemsExperiments]

Yes

[@sunainakash6475]

Hello, do we need to calculate blank every time when we make sugar working solution?

[@MicroChemsExperiments]

Yes.

[@bennylopez8128]

Can you tell me at which concentration the phelphthalein is? Thank you!

[@MicroChemsExperiments]

2%

[@maryroseveluz713]

Hello, i just want to ask if what's the name of the sample that has color brown

[@MicroChemsExperiments]

It was orange jam

[@nurulnadhirah64]

is it same as the starch content determinatio by using lane and eynon method?

[@MicroChemsExperiments]

Not same

[@skfun8418]

For blank reading you poured sugar standards solution in buret but what for sample... What should we pour in buret??

[@MicroChemsExperiments]

For sample titration, the same sugar standard solution is poured into the burettw

[@Tithaaa13]

Hello, thank you for the video it really helpful. I have a question about sample preparation, is it the procedure can be use to other method? Because i want to do phenol-sulfuric acid method with solid sample (biscuit). Thankyou!

[@MicroChemsExperiments]

Not sure about your method of sample preparation. You can try

[@bakacheyounes9325]

@@MicroChemsExperiments thank you so much please do you know dubois method?

[@learningagony4895]

How do you get the formula? Please explain. Plugin values is not quantitative analysis.

[@MicroChemsExperiments]

This is internally validated method.

[@mensreyrun7492]

Excuse me ! I want to know about the sugar standard , so why you choose sucrose for standard and Is it possible if prepared another sugar like (maltose fructose…)

[@MicroChemsExperiments]

SOP suggest to use Sucrose as standard. Don't know about other sugars

[@estebandxavieralvareztorre4231]

Why did you heated up the sample? Thank you and good day ;)

[@MicroChemsExperiments]

To facilitate dissolving the sample in water properly, and to release sugar molecules into the water homogeneously.

[@Nicha-qu3nw]

Thank you for this video , if sample is milk , yogurt can use this method ?

[@MicroChemsExperiments]

Yes. Same method will be applicable

[@negiboyalmora]

Hi this is very useful video. I want to know . How to determine sucrose percentage in chocolate powder. Please guide me.

[@MicroChemsExperiments]

We will make video for the determination of sucrose soon. Stay with us

[@AMalik-uu1oe]

Hi, will the procedure be the same for a liquid sample , for example fruit juice? Thankyou!

[@MicroChemsExperiments]

Yes. Same procedure should be followed for the liquid sample

[@pallavinawlakhe1122]

Aur jo liquid nhi hai jayse ki rice to usko kayse

[@huilynmak5928]

Hi, must the sucrose be dried before use? What if my sample is in powder form, must it be also dried before use? Thanks.

[@MicroChemsExperiments]

Sample should not be dried before the test. Sucrose standard shoul be dried before making standard solution

[@huilynmak5928]

Ok thanks. I tried your method, however my analyte is not as thick as yours. Hence it is difficult to see the colour change. Do you know the reason why?

[@MicroChemsExperiments]

@@huilynmak5928 Colour change depends on sugar concentration. Sample colour does not influence on the titration colour. Please perform standard titration and try to understand the color change properly. Then do the sample titration following my video strictly. You will get the appropriate results.

[@huilynmak5928]

@@MicroChemsExperiments thanks! I have a few more questions to ask. What happens if I don’t get a complete bright orange colour after heating for 3 min? I got a bright orange ppt with tint of blue during my experiment. Is this method possible to determine sample with unknown %DE? Thanks in advance!

[@MicroChemsExperiments]

@@huilynmak5928 Blue color should be removed completely which will give bright orange colour. You can determine DE% of unknown sample by this method.

[@hudarosley9857]

Hii sir/madam. Is this method quite similar to AOAC 968.28? How can I get total sugar results in grams, instead of percentage?

[@MicroChemsExperiments]

Yes. Similar to AOAC 968.28. You can convert the percentage by 100 to get the result in gram of sugar per gram of sample

[@hudarosley9857]

@@MicroChemsExperiments I see. Thank youuu. Then how about For sucrose, it shall be dried for how many hours and at what temperature before doing stock standard solution?

[@khushbukhan803]

Can I use my dried fruit sample for this analysis. Because I want to measure total sugar in my fruit species Please help

[@MicroChemsExperiments]

Yes. You can.

[@tusharnainani3368]

For biscuit, how should the sample be prepared ? Is centrifugation required ?

[@MicroChemsExperiments]

Grind or powder the biscuit and follow the instructions for solid sample given in this video.

[@pks3413]

have any gravimetric analysis of phosphorus in raw materials .

[@MicroChemsExperiments]

No

[@rajvivirani7755]

Which type of titration method is use here?? I used this method for my thesis..plz give me answers

[@MicroChemsExperiments]

Type not known.

[@sulltan2472]

I have a problem with the analysis of the percentage of fat , the results of one sample are different when i test it twice, whats the matter?! I am using gerber method … thanks for helping

[@MicroChemsExperiments]

It shouldn't be happened

[@fatimamusa1549]

Can I use this method for my dried plant roots and leaves?

[@MicroChemsExperiments]

Yes you can. But before taking for the sample, make paste of your sample using grinder or blender.

[@RonaldoRgftcut]

Is there official method or international standard method for Determination of Total Sugar_A Complete Procedure (Lane-Eynon Method) for food ?

[@MicroChemsExperiments]

This is the modified and validated Lane Eynon method

[@yousraboukssessa4849]

Please is this a dose of total or reducing sugars. Does it give the same results as a refractometer?

[@MicroChemsExperiments]

Total sugar. More accurate than refractometer

[@yousraboukssessa4849]

@@MicroChemsExperiments thanks a lot

[@nurizzah_]

Hi. May I know the reference for calculation formula?

[@MicroChemsExperiments]

Lane Eynon method

[@payalupadhyay9045]

Hello Sir / Madam Can I use this method to determine total sugar percentage in my Spices

[@MicroChemsExperiments]

Yes, absolutely

[@shirou.6243]

For sample preparation, why it must be filtered, and what the point for adding HCl, phenolphthalein ,and NaOH

[@shirou.6243]

Why, don't you just use the raw sample?

[@shirou.6243]

whyyyy

[@MicroChemsExperiments]

HCl is added for hydrolysis process to convert all Non-Reducing sugar into Reducing sugar. Filtration is needed to remove sample debris.

[@skyreacher4live]

Thanks for this. Two questions quickly: 1. Is it best practise to use a measuring cylinder to measure the stock solution used to make the working standard solution or to use a pipette or at least a burette? 2. What's the reference method used?

[@MicroChemsExperiments]

1. Best practice is to use calibrated pipette or burette. 2. Lane Eynon method

[@grownatural713]

Can we use this procedure for sugar content in wheat starch

[@MicroChemsExperiments]

You can

[@esraamossad3970]

How could I determine the D.E ( reducing sugars only not total sugar)?

[@MicroChemsExperiments]

We are working with reducing sugar. Please stay with us. We will upload soon

[@esraamossad3970]

@@MicroChemsExperiments thanks so much💫

[@MicroChemsExperiments]

@@esraamossad3970 You are welcome Ma'am.

[@gowthamimandapalli4974]

Hi can we use glucose to prepare standards solution

[@MicroChemsExperiments]

No. You have to use sucrose

[@nurulnadhirah64]

May i know the reason why

[@mariyanayab5171]

How Can we determine sugar content of feed?

[@MicroChemsExperiments]

Follow the method described in this video to determine the sugar content of feed sample.

[@izzahashimah4481]

Hello maam, do you have a video on determination of reducing sugar?

[@MicroChemsExperiments]

Not yet. We will make it soon

[@irshanapc6015]

How can we understant expected sugar concentration

[@MicroChemsExperiments]

Literature review, nutritional value standard/specifications

[@yousraboukssessa4849]

hello please I work on potato peelings can this method be valid on my sample

[@MicroChemsExperiments]

Yes.

[@yousraboukssessa4849]

@@MicroChemsExperiments tank you

[@sristivats1137]

Hello Can you make video on determination of reducing sugar ?

[@MicroChemsExperiments]

Okay. We will make

[@rajkumarjoshi6034]

Why we put solution in 60 degree centigrade water bath for 10 mins?

[@MicroChemsExperiments]

To dissolve sugar in water from the sample properly

[@vipinmishrarajnandgaon6859]

sir please make video of phosphorus content in oil sample.

[@MicroChemsExperiments]

Noted

[@VenkateshPhaltan]

Plz do video on Reducing Sugar

[@MicroChemsExperiments]

Noted

[@markjoshuabalingasa4528]

Can i ask where the other 100 came from?

[@MicroChemsExperiments]

One from dilution factor and another from percentage.

[@FST307]

share vedion on the determination of colouring matter in ready to eat food

[@MicroChemsExperiments]

Noted

[@rajvivirani7755]

This method is suitable for cold drinks?? Plz give me answer

[@MicroChemsExperiments]

Yes

[@rajvivirani7755]

Thanks.. give me a proper reference name plz..

[@pallavinawlakhe1122]

Aysehi oil ka bhi ho sakta hai kya

[@MicroChemsExperiments]

Not for oil

[@pallavinawlakhe1122]

​ bohot bar dekhi video lekin apne 5 gm liya hai 250 me to wo 50% kayse hua 2% hoga na Mera sample rice hai to kayse kre calculation me 50 kya hai

[@MicroChemsExperiments]

After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor

[@sristivats1137]

For how many days we can keep the stock solution

[@MicroChemsExperiments]

Don't keep not more than 7 days

[@sunainakash6475]

Please provide the procedure for Residual Sugars , i am in urgent need

[@MicroChemsExperiments]

Noted.

[@ritikachauhanvlogs175]

Ma'am make a video on reducing sugar in sugar syrup

[@MicroChemsExperiments]

Noted

[@anusharaut3449]

any hope for refrences ???

[@MicroChemsExperiments]

Lane Eynon Method

[@anusharaut3449]

Thank you ! But Reference of Microchems experiment !

[@nabilakhilwa2591]

Is total sugar same as dextrose equivalent?

[@MicroChemsExperiments]

Yes. Same as dextrose equivalent

[@nabilakhilwa2591]

@@MicroChemsExperiments if i use maltose as sample, the standard sugar solution is also sucrose or other sugar??

[@MicroChemsExperiments]

@@nabilakhilwa2591 Standard should be Sucrose

[@nabilakhilwa2591]

@@MicroChemsExperiments okay thank you very much 😃

[@MicroChemsExperiments]

@@nabilakhilwa2591 You are welcome

[@rajesh24292]

Dear sir/madam can you have any method to find out bypass protein and fat? If you have please mail me I need this urgently Thanks.

[@MicroChemsExperiments]

Sorry Sir, we don't provide any soft copy of any method.

[@rajesh24292]

@@MicroChemsExperiments oh thanks Sir/madam.if possible please make one video on this also. Thanks

[@MicroChemsExperiments]

Okay

[@pallavinawlakhe1122]

Original sugar concentration 5gm 250 me yane sirf 2% hua to 50 % kayse hoga 50% btaya hi aur 5ml dala hai final sample

[@MicroChemsExperiments]

After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor

[@poonamprisha1410]

From where this method took plzz reply is there AOAC method

[@MicroChemsExperiments]

It is called Lane Eynon Method, You can use AOAC 923.09 as reference.

[@poonamprisha1410]

@@MicroChemsExperiments sir muje mila nh y method iss reference par plzz aap iski pdf share kr skte h AOAc se

[@MicroChemsExperiments]

@@poonamprisha1410 We can't share the pdf. Sorry for that.

[@poonamprisha1410]

@@MicroChemsExperiments hum iss test ka reference AOAc 923.09 de skte h is this feasible for NABL accreditation plzz reply 🙏

[@MicroChemsExperiments]

@@poonamprisha1410 What is your test parameter? write in full.

[@induriramramesh756]

I have simple method useing Fenhling solution

[@MicroChemsExperiments]

Share your protocol to our mail. Thanks

[@abuayan8476]

Good job but music makes your video poor.

[@MicroChemsExperiments]

Ok. We will make the music lower from the next time

[@pallavinawlakhe1122]

Ye procedure ka concentration galat hai sample titration me colour hi change nhi hota reply to do bhayya

[@MicroChemsExperiments]

Try to follow this video strictly

[@pallavinawlakhe1122]

​@@MicroChemsExperiments bohot bar dekhi video lekin apne 5 gm liya hai 250 me to wo 50% kayse hua 2% hoga na

[@Ccrytzz]

Can I use Glucose instead of sucrose?

[@MicroChemsExperiments]

No.

[@nurulnadhirah64]

May i know why can't use glucose instead of sucrose?

[@poonamprisha1410]

Plzz Reply sir it's urgent for me

[@MicroChemsExperiments]

Lane-Eynon Method

[@poonamprisha1410]

@@MicroChemsExperiments sir I kwn this is lane eynon method but as per fssai manual is Too tough test but apne jo krvaya vo muje bht acha but iska written material kha se milega jiska reference de ske plzz urgent reply

[@MicroChemsExperiments]

@@poonamprisha1410Sorry we dont provide any written materials.

[@pallavinawlakhe1122]

Calculation me 50 kya hai reply do plz reply bhi nahi dete

[@MicroChemsExperiments]

After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor

[@pallavinawlakhe1122]

Ek point smjme nhi aya kya tum bta sakte ho reply plz.....

[@MicroChemsExperiments]

After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor

[@suneelashahzad1756]

Why we release 17ml?

[@MicroChemsExperiments]

To finish the titration very fast as we know that about 22-25ml standard solution will be needed for that titration.

[@suneelashahzad1756]

@@MicroChemsExperiments got it thanks

[@suneelashahzad1756]

@@MicroChemsExperiments I have many questions regarding experiment so how can I contact you

[@MicroChemsExperiments]

@@suneelashahzad1756 You are welcome

[@MicroChemsExperiments]

@@suneelashahzad1756 Find me in our facebook page. Link is given in the description section.