I am jealous of your students! That is a beautiful explanation, thank you so much.
@carinaashcraft1627 Жыл бұрын
just watched 3 other vids trying to understand this, and this one helped me get it right away!
@triciaparaiso93793 жыл бұрын
I watched so many vids about getting the charge of the peptide, and this video clearly explained it!! I can now answer my homework! thank you so mucchhhh
@mrzed2614 Жыл бұрын
Short, simple and well explained. Great job :D
@justinjoseph25313 жыл бұрын
THIS PROF IS SO GOOD!!!! My prof explained only what PI was but I had no idea how to utilize in peptides. Thank you so much!
@nidhibawane11528 ай бұрын
Thank you for solving my problem 😅
@MingeTheAllKnowing3 жыл бұрын
gurl u just saved my life
@justinelewicki91695 жыл бұрын
Thank you for this video! Your method really helped me understand the process!
@khayden7775 жыл бұрын
Thank you! That is so great to hear! Good luck with your studies!
@RethaSetiawan4 жыл бұрын
Thank you so much! The titration scheme is a helpful method. Could we also appreciate that she's writing mirror-inverted
@khayden7774 жыл бұрын
Actually I am writing normally. I mirror flip the image when I edit the videos prior to publishing them :). However, I have also seen people record off physical mirror as well when making these videos.
@noneofyabusiness10985 жыл бұрын
thank you so much chegg just told me to only use two for a similar question without explaining why those two. but this literlitly explained why
@khayden7775 жыл бұрын
So glad you found it helpful! Happy studies!
@osinachiakaluka11165 жыл бұрын
This is amazing....thanks Kate!!!!!
@khayden7774 жыл бұрын
Thank you!
@heisenburger3667 Жыл бұрын
Where did the N-terminus 9.0 pKa come from? N group from Proline?
@alinadunkel-wy5hk11 ай бұрын
Gran video, mil graciasss
@TaeThei3 жыл бұрын
May I ask, what if an amino with an ionizable R group is repeated? So lets say two Lysines in a polypeptide?
@khayden777 Жыл бұрын
Hi Tae.. since both ionizable groups will be lost at the same pH, then you lose both charges (or gain both charges) when you cross their pKa. Their charges still add to the total (i.e. both will give a +1 so two lysines will give a +2.. but when you cross their pKa you lose both so go from +2 to 0... I hope that makes sense).
@Getencourageddd6 күн бұрын
so good
@POKEMONMASTER23able3 жыл бұрын
Thank you so much for this ! 😭
@abhishekbehera75143 жыл бұрын
Thank you ma'am .
@FariaKhanSinthia2 жыл бұрын
Thank you so much
@shashwatgaur72583 жыл бұрын
Thank you very much! I'm new to the subject. Can you recommend some good books to start with?
@khayden7773 жыл бұрын
We use Foundamentals of Biochemistry by Voet Voet and Pratt in my class
@shashwatgaur72583 жыл бұрын
@@khayden777 I know I'm replying a tad bit later (my apologies), but thanks for the recommendation!
@soumyajitchakraborty83092 жыл бұрын
Thank you 😁
@kiban76762 жыл бұрын
6:11 - I dont get this part ... HELP !!!!😪
@marin92073 жыл бұрын
Ácids And bases have ionizable groups but polar and nonpolar don’t have
@asyareedus3 жыл бұрын
what if they give us three pKas for the basic/acidic aminoacids? which ones do we use for that specific aminoacid then?
@khayden7773 жыл бұрын
For constructing your hypothetical titration: If it is a central amino acid, then you include the pKa of the R group as it is the only free ionizable group. If it is a terminal amino acid, then you include the pKa of the free terminus (whether it is the C or N end) and the pKa of the R group.
@asyareedus3 жыл бұрын
@@khayden777 no i mean they give us 3 pKa s for each of the amino acids (central and terminal ones) and none for the COO or N terminus .. )obviously bc they already gave us the all pKas .. im sorry im german and ive been looking for this kind of exercises in german but couldnt find any and this is the first time im seeing the question being solved right
@khayden7773 жыл бұрын
@@asyareedus ah, so you would follow my protocol, Ideally, you would be provided the N and C terminus pKa's. They don't fluctuate too much based on the identity of the peptide, so most instructors use 10 for the N terminus and 3 for the C. However, we know they do vary slightly... but it is a safe assumption especially if the isolecetric point exists between 2 R groups.
@asyareedus3 жыл бұрын
@@khayden777 yes thank you, i ignored the low pH pkas that were given for the N terminus aminoacid and the otherway round for the C terminus.. (bc those were the ones for groups that were involved in the peptidbinding) thank you ur video rlly saved my exam :)
@eviekins74963 жыл бұрын
I love you thank you
@zexicc3 жыл бұрын
Why not Ser? He has OH group...
@khayden7773 жыл бұрын
Yes, but its pKa for the hydroxyl group is around 13. Typically much higher than biologically relevant, therefor most textbook authors put serine in the non-ionizable hydrophilic group of amino acids. That being said, there are plenty of enzyme mechanisms that rely on a coordinated effort of intermolecular interactions to deprotonate serine for covalent catalysis