DNA extraction from Blood

[@MJ-ye7dd]

The best ever, so professional and clear.

[@samrakhan4408]

So well explained!

[@nandadeep999]

Very well explained. Please give little brief about its applications in pharmaceutical

[@pseudolichiacoliaerogenes3451]

Thank you for this amazing and well organized vid Professor!

[@NazriB]

Lies again? Project Artemis Blood DNA

[@datisalaee4693]

Very helpful presentation. Thank you.

[@chubiin20s]

This looks very different from the method I performed: salting out method. I added RBC lysis buffer + Trenton and centrifuged at 10,000 rpm for 10 min. Then collected the pellet and discarded the supernatant. Then added WBC lysis buffer + SDS + Nacl and centrifuge again. Here I collected discarded the pellet and added chilled 99.9% ethanol. Centrifuged again and lastly collect the pellet, air dried and added TE buffer and stored at -20 degree Celsius.

[@moemoethecoolest]

I don't know how I got here because I'm just a regular person but I still think this is cool. I want to study biology. I wish I could work in a lab but I'm very clumsy so I think I'll be a writer instead.

[@qaisariqbal6758]

Superb explanation

[@medicalacademy8135]

excellent presentation many thanks

[@EmmanuelAsiimwe-ey6fj]

Well organised and presented

[@Daniel_loung]

wow, i am so Passionate with this, but i cant do what i want because i have anxiety disorder, i always shaking hand during the experiment, i got rejected from other laboratory so many time, anyone can share experience or advice for me. Best regard .

[@chemistryinstruments7156]

Maybe you can see a neurologist first to get an idea what is going on. I had some problems with that also, some lab stuff needs some finesse and it makes me nervous sometimes. Drugs are an option too for anxiety. I'm sorry it may involve doctors which are expensive sometimes.

[@markgearing]

Identify what it is about this process that you’re passionate about. Is it working in medicine? Is it assisting in treatment? Is it process? Something else? Once you know what inspires you look for alternative roles with similar inspiration in which your anxiety isn’t detrimental to the outcome. Addressing your anxiety will be helpful in many aspects of life, but it’s a big world with many roles available. You don’t have to do that job; find one that you’re ready for. And remember, whatever you start with is just a stepping stone to your next role. You can work on reducing your anxiety and expand your skill base at the same time. Or one at a time. Whatever works for you.

[@sunnymadar4056]

Learn art of living workshop do sky process with me also same thing happened but after practising sky technique I got selected and that is now thing of past , I got good job too 😌

[@hrahselmuanchhana8359]

Smoke weed everyday

[@nwaobigift9924]

Just try to be bold and focused. Don't be scared of making mistake. When your mind is comfortable and not scared, your hands won't shake. Pron you're afraid of the people in your surroundings. You have to work on that. Train yourself to not be scared of them. Because you're all learning and everyone makes mistake so please just try to first grow ur confidence.

[@Lifescience_aspirant]

Please make a video on DNA isolation from saliva samples

[@priyam8261]

Thank you very much. Nicely explained

[@HAUWAUMOHAMMEDBELLO]

Thank you so much 🥰

[@yenantruong987]

Thanks for the creative video, kindly you please share the ingredients in the buffer AL, AW1, AW2

[@ahmetziyaaktas9179]

So nice

[@evaberger1628]

So helpful! Thank you

[@natpethunai3964]

great job docter

[@ammarkareemrashidghareeb5762]

excellent video. thanks a lot.

[@moamenmetwelly1898]

Great work👏👏

[@marwanj.r2513]

Wow my alaah bless u 🤩🤩🤩🤩

[@ms.kalico4489]

That was a very good video!

[@MagajiBadamasi]

Awesome

[@erinwusiblessing9262]

Very helpful Thank you so much.

[@muhamadjameel1517]

appreciating ,,, hafiz waheedullah

[@harshkumaragrawal5278]

THANKYOU SO MUCH MAM I AM TRYING TO THE BEST PERFORMES

[@Tepmodify]

Good video.

[@huntergaming9793]

I fell in love with you lady how well you explained it 😘😘

[@mouhamadoumoustaphadiop8024]

I am very glad for this movie Thanks

[@droneasmr]

This may be a stupid question, but is this is DNA extraction from the white blood cells of the blood component, right?

[@shimashima8585]

I like it i need a PDF of it.

[@mhdamins]

amazing thank you so much

[@parsasanjana7813]

Hello, when you draw blood from a volunteer, should the samples be kept in ice, in case, one has to travel from the hospital to the lab? ( to avoid DNA degradation). I am confused since I read direct contact of blood with ice causes damages WBC.

[@priyankaflorina8411]

you should keep it on the ice after 10-15 minutes prior to withdrawal.

[@noorpk]

You may use lysis buffer, 2X concentration, then you can keep the it for longer times

[@khushboogautam6730]

Hii ma'am I have done as same all the procedure with my blood sample but my conc is very low like 5 and 7 ng/ul and one more thing some time ratio is above than dna means rna

[@harrison7952]

How do i modify this protocol to extract DNA from dried blood spots?

[@kingofnuclearfallout39]

Go to some DIY video type in gene editing DIY it's easy just the typing part good luck

[@lavanyamadhusoodanan3432]

If I add proteinase k just the double amount..suppose 40ul instead of 20ul.. to blood during DNA extraction..then what happens?

[@Tepmodify]

Clot in column tube. Trableshooting

[@ivanrafael4472]

hi, why u did not use buffer ATL

[@noman12310]

Thank you

[@samanaguib5063]

im watching this for fun for no reason

[@liveisnice9510]

Thank you doctor

[@paulidam6582]

Nice

[@zebafatima4462]

Maam we use ssc(mixture of sodium acetate nd EDTA) buffer instead of proteinase k

[@francestolentino9396]

"Running buffer in this case is 1 times TBE containing a 30 ????? is added to the tank" at 9:13

[@kristtyguevara7497]

Ethidium bromide

[@eshetuseid3337]

Perfect ,except for the a noisy background instrumental. Thanks with alot of respect!!!

[@eshetuseid3337]

...apologies for the typing error

[@hazlanshah]

Hi. May I know how do you create the animation in the video?

[@hamidizouina4787]

MIS7 i am asking same question

[@snehajpraj9100]

Thank you....

[@deepalibhardwaj2696]

your welcome

[@rizkard311]

So is it okay if the blood sample was lysis in the EDTA tube?

[@r4rallinone64]

Thanks you

[@hananeboukrid880]

thanks

[@Somehackcopiedmyuser]

My Centrifuge max spin is 7000 RPM, will this be sufficient for this protocol?

[@erysaputra8995]

is this the same procedure used for extracting DNA of SARS-CoV-2 diagnosis with PCR?

[@sumaiyamansoor7784]

No. The PCR test for SARS-COV2 requires RNA extraction from blood since the virus is an RNA virus it does not have any DNA.

[@sheereen-gy9ih]

How the specific gene or species chromosomes whats getting affected in human

[@The_Eminence]

very interesting

[@josephwhite4933]

Am I able to just use a centerfuge

[@heshamalsayaghi1013]

Dear:what mean Operation Profile (OR) . In blood Groub . O rhD Positive. Please tell me if you have experince about this.

[@nexigent]

I want to look at my own dna from my blood how can i do that?

[@leah8238]

What kit do you use?

[@MJ-ye7dd]

I saw QIAamp DNA Blood Mini Kit

[@azlieabd]

I loveeee it.

[@DarlinMarleneVeVo]

nice, thanks

[@lianaa8629]

What is the size of DNA ladder?

[@apol8245]

ay yo next time i bleed ima take some dna out: 🧬

[@patrickchibwe8716]

Great

[@nikita8927]

which method is this??

[@naimjelahastu3669]

is there any lab report for this hehe I'm just asking for friend

[@ayeshaafzal8450]

Yes

[@zainabafzal9613]

@@ayeshaafzal8450 can you please share that report with me. I urgently need it😬❤️

[@zyyma6802]

@@zainabafzal9613 same hereee

[@zainabafzal9613]

@@zyyma6802 I have it. I can send it to you. Just give me your email or something

[@drproductiontv8593]

GREAT

[@mehrunnisaarif2191]

how to prevent the lysate in purification column from thickning?

[@zapp1984]

Anti coagulant in the draw tube. Should be already taken care of before you get the sample.

[@IamAshokKumarSingh]

How I found dna fo onion plz make a video

[@ayeshanawaz4206]

what are consumable in DNA extraction

[@JoeMama-kg9lx]

Esha Nawaz the dna is, but make sure to let it dry

[@nashidsultana7689]

why the buffer AL is so frothy😔

[@derek3052]

How to extract DNA out of a bone Can we really extract DNA from bone or not can you please tell me

[@TF2Scout..]

We can

[@derek3052]

@@TF2Scout.. Oh ok thx

[@headdragondavidaustinsimmo4025]

in Bakersfield California there my grandfather's skull chopped in half worth millins is Samuel prophet reborn

[@xcp4518]

Thanks David very cool

[@sashamuller9743]

where can i learn genetics to be able to understand this?

[@victoriarichard3468]

Same with I, honestly seems I will save probably will be useful in future

[@ImNotQualifiedToSayThisBut]

Pharmacy, chemistry, biology, biochemistry etc. Enter university or learn from free resources online. Some universities offer their lectures for free only downside being you can't actually attend exams and graduate but for autodidactic learning there's enough resources.

[@مختارحمودرمضان]

❤❤❤❤❤❤

[@GursewakSingh-py4wi]

how much it expensive process. please tell the price.

[@Sharkyktc001]

A qiamp DNA kit has enough reagents for 50 extractions, costs $259. So excluding the quality control steps and other consumables used, that's $5.18 per sample.

[@linamahmoud651]

so isn't it written in the SOP ?? what is the benefit of watching this video '?

[@headdragondavidaustinsimmo4025]

i need blood test

[@sharifulislamhridoy4122]

best

[@amirulafiq1365]

nice

[@tanmoysaha2490]

nice.

[@ankitbarnwal4328]

Wats this aw1 and aw2??

[@Hey_reema]

Wash 1 & wash 2

[@pitou4108]

kumpliketed

[@wingsofgalaxystarunlimited6160]

ssoooo pro

[@ragavipriya08]

It will take 1yr ... 4me to learn this lengthy... Technique😆😂😂

[@sailplaneFan707]

I am going to interrupt incidentally

[@aliarshadali8019]

السلام وعلیکم ورحمتہ اللہ وبرکاتہ ھو جناب محترم کیا انسان کے جسم کے پسینے سے ڈی این اے ٹیسٹ سے جنس عمر نشوونما اور غذائیت زنا اور دوسری چیزوں کو با آسانی معلوم کیا جاسکتا شکریہ

[@bruhbruh1123]

kendrick lams

[@silviaalexandrecordeiro8400]

🌹🌎🌍🌏🌹

[@Arbulatov]

What can you do with extracted DNA?

[@amortality999]

eat it and youll have the guys powers?

[@akumar7298]

Ratna singh

[@sandeshbme]

Extracted DNA is used in applications related to molecular diagnostics, Genetics, Forensics, Even in developing vaccines like for eg corona virus

[@abdulezizmuhemmed6279]

u can use it for DNA fingerprinting and capture the criminals.

[@sharmeensarfraz8198]

Why Again and again repeat same process 🥺🥵🥵

[@commonsensedailypod]

Bru i study linguistics what the fuck on earth am i doing here at 3 am

[@gabrielavelandia526]

No entendí

[@bobbybrownfromchinatown]

z-DNA @0:00

[@mominjaved7616]

Don't use music at background.. Its haram in Islam

[@headdragondavidaustinsimmo4025]

doctor said 200.000 in 80s i seen it im telepathic i asked him to bring me and kids witnessed it 30 kids

[@AndruideAtario]

"please note that blood sample collection should always be performed by a --- plubottelist --- ?" What the fuck, not even 30 secs into the video and already fucked up.... :((((

[@headdragondavidaustinsimmo4025]

im in gold beach Oregon i have b+ blood half DNA of man im adam yoseph Joseph Moses Ishmael Gidian im Jesus Bethlehem son of David Mike Simmons died Winston Oregon every storm and earthquakes happens the same day i was crucified0000 A.D. jews were forced to keep B.C. records along side A.D. tell my brother Jesus Nazareth came

[@sourabhbabu2900]

nice

[@ZERODEGREE82]

nice