Using a Micropipette - University of Leicester

A short film showing how to use a Gilson Micropipette. The film was made to support the teaching of undergraduate Biological Science students at the University of Leicester.
The film was produced by GENIE, based within the Genetics Department at the University of Leicester. Further information and materials can be found on the GENIE web site www.le.ac.uk/genie and the GENIE VGEC web site www.le.ac.uk/vgec
The film was directed by Jon Shears of ITS-Multimedia Services and the film was produced by Raymond Dalgleish, Nicola Suter-Giorgini and Cas Kramer.

[@cubehire3653]

This is the best Pipette instructional video I have found thank you.

[@ceciliarosenqvist9797]

Cube Hir. e

[@DA-js7xz]

I laughed so hard at that sound effect for the "Don't Do's".

[@nicholaspark4165]

The Nos sounded like a broken tuba or a fart

[@burcusatelier]

me too!! the sound...was great! :)

[@seven7wenty]

haha that's how you'll remember the do's and don'ts

[@valeriozippo5720]

poooo poooo X .......... LOL

[@cmrnqueen]

only pipette video that shows how to read the the P2...Thank YOU!!!...just saved me from getting hell from my lab proff

[@willdrew6825]

This video made me want to micropipette some liquids so bad

[@HZAexNB]

Not as easy as it looks mate

[@denwin4221]

No bro What my teacher could not explain Like first stop and second stop Easily explained by the mam ,for me to understand. Really helpful ❤️

[@melissarichten6323]

Same

[@Dinlek]

Without a doubt the most helpful and informative lab video I've watched. Thank you very much.

[@dontownsend9724]

You've covered the possible ways the pipette can be contaminated or damaged,but you've failed to follow up on what to do about it. I started working in an analytical lab over 20 years ago and they were having trouble passing the unknown quantitative metals tests each year required by the EPA. My first year on these analysis's I was testing the output of my eppendorf pipette on an analytical balance with reagent grade water to insure in was delivering the proper volume before making my standards. Another analyst asked me what I was doing , I told them and they then said." well I worked in the medical field for over 10 years and we never tested our pipettes". All I could think was no wonder so many people die in hospitals if this is normal SOP in the medical field. If the pipette is found to be not delivering the set volume it must be taken apart and cleaned or repaired !!!! After this all our measuring equipment was checked closely for accuracy. We never failed our yearly test again .

[@TheSnakeboy11]

Awesome story, that's making the world a better place.

[@jauld360]

The first thing I did on receiving a new pipette was to test it. a) for accuracy, b) for repeatability and c) to check my technique. That analyst wasted 10 years.

[@gilong1311]

Awesome video, answered all my questions.

[@chriswong1516]

How a clear explanation. I'm going to do laboratory practical tomorrow. This may help a lot to me.

[@김규형]

정말 유익해요! 과학 실험을 진행하는데 필요한 기본적인 지식들이 알차게 들어있네요!!

[@christyxoxo]

lecturer told me to watch.. and i found it very useful!!! Thanks!!

[@johannesmthembu8663]

Thanks University of Leicester for this video,well explained thank u.

[@jtxc3]

Thank you! This is brilliant! It helped me review for my lab mid-term

[@austindiehl6540]

This video was terrific. Thank you

[@KulbirAhluwalia]

This video is so good 😍😍😍 Covered the smallest details Awesome work

[@ifeyinwaobikwelu1314]

A Very Good video, detailed and well explained. this video really helps. thank you

[@marcelomesa2154]

Excellent video, thank you for bringing such a great content, University of Leicester! Subscribed : )

[@oreadeboye4506]

Thank you so much for the video. You made pipetting so easy to understand.

[@akritino7437]

Thank you for such an informative video ! Loved it :)

[@cherryy_2468]

this was honestly a really helpful video thank you

[@quack703]

This video truly helped me! Thank you!

[@anjichant543]

That was an excellent revision of my 4 years of study in lab.

[@Nayyarscorner93]

greatest demonstration ive evr seen good job

[@fabyalauture5929]

Thank you this video explain the process of pipetting very clearly

[@lizziesinclair4254]

Thank you so much for making this video!

[@KhanaHatake]

I had to watch this for uni, but it's actually so interesting!

[@nayabghazali5455]

thats the best video i hv found so far!

[@korenmoscovich4681]

Literally the best pipetting video ever

[@raghadalani9706]

Nice work.. I understand now... Thank you very much 👍👍👌😊😊

[@mohammedahmedjalloh531]

This video is super helpful... so very detailed

[@FieldGamers]

This was perfect. Thank you

[@gabvela8192]

Excellent explanation thank you!

[@yolniejacques8117]

This video is very helpful, thanks!

[@cherylpatel8197]

Such a helpful video! Very thorough.

[@fireemblem2770]

I agree!!

[@palashmassey]

wonderful...love it and learn it..keep uploading please

[@Hiphop101ize]

Oh yeah, all this talk about the tip got my body tingly!

[@winterhatharrived2037]

P2 range: 0.2-2ul P20 range: 2-20ul P200 range: 20-200ul P1000 range: 100-1000ul (in this case u is the symbol for micro, or 10^-6)

[@Sp-fe1eo]

thank you so much for the video...it's really cool and easy to understand

[@Evemaru2]

Best video tutorial I have seen.

[@chauhuynh8158]

Wonderful video, thank you for helping me with my lab

[@bottledblue]

thanks heaps - helped my prepare for my first molecular biology lab! :)

[@xeryd6037]

same but 6 years later

[@nafis_rk]

@@xeryd6037 same but a month later

[@retinitispigmentosawillbec6447]

Thank you so much! A very helpful video!

[@zahidwajdan2672]

Best Explanation, and of course Sound Effect.

[@kkmatt]

¡Muy excellente! Totally ready for lab!

[@lglintott]

Brilliant. Putting this on my teaching lab page.

[@BioLogicalNerd]

Thank you for this, I'm a micropipette pro now!

[@hazelezeribe5767]

Soooo Helpful!! Thank you: :)

[@natxnatxnat]

What a brilliantly useful and helpful video. Thank you!

[@noshinnamira6608]

this is so detailed, i learned a lot from it

[@hamismwinyi3723]

Thank you, this video is really helpful

[@TheSexyN3RDZ]

"puhpet"

[@essentialsofbiology1643]

Puppette! It's French. 'Whilst' is the worst. Why not 'while,' dear Brits et al.? But excellent video, thanx Leicester.

[@lalayatem3758]

amazing video , thank you and God bless!

[@KingofDundee]

Sounds like Douglas from the IT Crowd, great video!

[@selamyemane1640]

You have been very helpful. Thanks

[@mikejedi182]

Thank you for creating this video

[@ifrahjameel4588]

best explanation so far

[@minasdrakos7544]

excellent, very analytic!

[@sobster123]

Best pippetting vid out there!

[@yeyhu4767]

excellent video, thank you !

[@microfetish]

A lovely job indeed!

[@brookebain7250]

Thanks for this video! My professor didn’t go over how to set the volumes and I was so lost

[@liying1994]

Excellent video.

[@lyubomirgizdarski8191]

Great video!

[@sarahjt1]

Very helpful! Thank you!!!

[@fatimainigo25]

Best Video! Thank you very much!

[@thanhmiennguyen895]

Thank you so much. It's useful.

[@LoveResidentEvil4]

Thank you :) helps my lab bio a lot :)

[@JustAethnicDonut]

Thanks, so helpful

[@ayeshashaikh9729]

Thanks a lot for the best video 😊💗

[@andraskovacs5247]

fantastic video

[@JoChen-gb4xr]

honestly, the part which the video demonstrates dos and don'ts is the best

[@lucysnowe9974]

Very useful tips, thank you. :)

[@razahasanbhatti8454]

Thank you very much very informative video. kindly tell me how to pack culture of nitrifying bacteria in sashay or a bottle actually i am asking about the media some people pack bacteria in powder form which media they use to bring alive bacteria into powder form?

[@Densitometry]

Amazing tutorial.

[@KeptNow]

not bad for an instructional video. these science instructions are usually 20 years old and out of date. this video was really well done--with camera angles, explanation, and reasonings

[@riyadhdahham2075]

Cood... amazing explains Thank you شكرا

[@Teo_live]

Great video. The [X] don't-do's were entertaining

[@fisslimen]

Great help!

[@huongnguyenvan4020]

thanks! it is very useful!

[@joanne7699]

Hi may I ask does the Pipette need to be vertical at all times even when dispensing liquid and Does the pipette tip need to be against the wall of the receiving tube during dispensing ? Thank you

[@elenaautumnalmermaid6913]

As a biochemistry student, my heart breaks everytime the liquids touch the actual pipette. But great video for learning what to do and what not to do.

[@deadbzeus]

I feel this lol

[@nxph2108]

So satisfying.

[@cutler610]

awesome vid thank you

[@victorenriqueriosortega5917]

Es un video muy interesante sobre el uso de las micropipetas, desconocía qué algunas pipetas tenían en el botón un número lo que nos dice hasta cuanto puede medir, además de los números rojos qué también sirven para lo mismo

[@sasa012]

great video ...

[@Navid115]

awesome video

[@rabindrakhatiwada3558]

is there genetic engineering lecture series? if yes than please do post it.

[@charlesvictoria6800]

good video, thank you!

[@ayomideolorunfemi]

such a good video

[@kirad1947]

I have a Biology lab pre-quiz tomorrow, haha thank you

[@CWGminer]

Same

[@a.s9509]

If we have to use the second stop to eject the volume into the receiver tube there is going to be bubbles with that which is going to give you inaccurate results when say you are measuring the OD of serum samples. And if we don't use the second stop there would still be liquid in the pipet tips. What do we have to do?

[@spkspk1416]

Literally I found gem in the form of this video

[@soulijm1757]

Thank you so much

[@bhaktisatam726]

Thanks for risking your pipette for us

[@sharon_ng]

+UniversityLeicester How should we clean the cross-contaminated pipettes then?

[@nadew.02]

Thank you so much

[@estelamestellaestellaiscry4417]

Excellent PLEASE .give examples of calculation of valume...

[@roisfatawy1712]

Thank you. You explained properly..

[@elakkeya4173]

Very helpful

[@chillagunilla]

Great for preparing blotters!

[@vanniah1]

Thank you!!!!