DNA Replication | Prokaryotic vs Eukaryotic Enzymes

In this video of DNA replication we have discussed about the enzymes involved in the Prokaryotes and Eukaryotes.
n DNA Replication , the Single DNA molecules having two strands is replicated to form two DNA molecules…. we see we having DNA molecule,its two strands acts as Parent strands to make two DNA copies ,so on these parent strands the enzymes and proteins will work on to synthesises the daughter strands on both the strands ,so that in the end we get two identical DNA molecules.

[@biologywithmayank364]

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[@Shine_On-t1s]

Amazing representation! Thanks a lot. You got a new subscriber as i instantly subscribed watching your first lecture just for one minute . It shows how your efforts can attract students, keep going.

[@hussainbiology]

Awesome, thank you!

[@hope5746]

Thank u sir for your videos on replication , transcription and translation ...it helped me a lot during my exams ... I am an education student and I had biochemistry as my elective paper for 2 semesters and I had failed in sessionals untill I watched the whole series ...thanks a lot....

[@hussainbiology]

Thanks Asha for appreciation..Glad to know that it helps ✌️ 😊

[@srikalluru8690]

By seeing the difference between eukaryotic and prokaryotic DNA replication.. Enzymes involvement give me a clarity.... It is soooo help full for me... 👌👌👌👌👌👌👍👍

[@hussainbiology]

Thanks Sridevi for appreciation...

[@sandeepsah4937]

Pol €( pol epsilon) - it is recruited to complete DNA synthesis on the leading strand. It is associated with Proliferating Cell Nuclear Antigen(PCNA) protein that serves as a sliding DNA clamp.

[@williamcowan4936]

I've read that it's delta that is associated with that! am I wrong?

[@tanvirahmed-rr7vt]

@@williamcowan4936 dna pol epsilon is more suited in that functioning than delta.

[@doctornorthern]

​@@williamcowan4936 New updates are different He js right Epsilon enzyme works in Leading strand and delta works in lagging strand.

[@Snowman61785]

The best summary of the comparison between DNA replication of prok and euk. thanks

[@hussainbiology]

Thanks for appreciation..Glad to know that it helps ✌️

[@masroorkhalil9655]

you have made easy this whole book like question in one minutes thsnk you bro

[@happychemist]

sir you have all my respect, thanks for share this. I hope you always be happy and stay healthy

[@hussainbiology]

Thanks dear for appreciation .... Glad it helps...... Stay Blessed

[@priyaprasad4237]

Explanation is up to the mark but written part is not seen properly for reading.

[@anushkasupakar3305]

Great way to be involved in some learning during self-quarantine period! (Stay safe. everyone!

[@hussainbiology]

thanks Anushka for appreciation..Keep learning , keep sharing and stay safe.✌️✌️

[@_atifch]

nice sir good job keep it up

[@apoorvajoshi5867]

Thankyou so much, so helpful ❤

[@hussainbiology]

most wlcm... keep sharing and supporting

[@kiarahumphrey4105]

Thank you for explaining. So helpful!

[@ammarahmehak9986]

Excellent and ossum

[@hussainbiology]

Thanks Ammarah for appreciation... Glad to know that it helps

[@pubgedits4883]

Satisfactory video 👌👌👌

[@sandeepsah4937]

Superb explanation 🤩

[@dyaneshwarsontakke51]

Best videos...,👌👌👍🌹💐

[@hussainbiology]

thanks

[@ammaramehak9473]

good and nice explanation of replication.

[@WasimAkram-tk9sh]

masallah...very good job ..that will b the very helpful for csir net lifesc aspirant

[@hussainbiology]

Thanks for appreciation...And yeah all my lectures are directed for CSIR net.

[@WasimAkram-tk9sh]

@@hussainbiology thanks

[@goldimehranegi1019]

Your voice imaging sir.. and ur explanation technique is supper✌👍👌👏

[@hussainbiology]

Thanks buddy for appreciation.....Glad to know that it helps

[@yaaansndgn2391]

Thank you sir for this. Truly a life saver 😁

[@hussainbiology]

You are welcome ✌️

[@shivanimishra2045]

I have read in Lehninger, latest edition Epsilon Polymerase is involved in Leading strand synthesis, please go through this, and do a bit correction in video

[@hussainbiology]

Most of the books have mentioned what you are saying but 2015 recent study has shown what i have mentioned. www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/

[@ileanaascencio2464]

Soooooo good and clear Thank you!!

[@hussainbiology]

Thanks Ileana for appreciation.... Glad to know that it helps

[@sayedsajid2529]

Osmly explained ❤️

[@SR-ok3ym]

Plz make videos on.. Cell all cycles and proteins

[@yosef4742]

Thank you Hassan bhai

[@ashutoshkosariya191]

Sir could you plz tell me witch books should I prefer to get some more detail knowledge about this topic

[@hussainbiology]

✓Lodish Cell Biology ✓ Alberts Cell Biology

[@uthandolwethoo]

greatly explained i have no questions at all now, if only it was a bit slow though

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@misbashabir2063]

Recently I learned that Dna Pol delta synthesize lagging strand ....and Dna Pol € synthesize leading strand ...m I wrong ???

[@hussainbiology]

www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/

[@Amritpalsingh-do5yv]

Nicely explained... Sir Your video on supercoiling is also required..!!

[@hussainbiology]

thanks for appreciation...Glad to know that it helps

[@yauheni7429]

thank you very much for DNA replication series. Very informative, very accessible form in which you've presented such a hard topic for me. Can you answer a couple of questions, please? 1. As one of the commentators has already said that eukaryotes primase activity is built in alpha-polymerase, can you clear the telomerase replication plz? during this, the primase is also a subuinit of the alpha-polymerase or it is some kind of special one? 2. As far I can understand eukaryotes have a clamp loader and sliding clamp which are independent from delta- or alpha-polymerase and prokaryotes have gamma and beta subunits of polymerase III for that. Is that correct? 3. I understand that it might be a complicated one, but I don't grasp whether there is any difference in the unwinding of dna (during initiation) between euka- and prokaryotes. For the exception of that pro's have gyrase and euka have topoisomerases. I mean the negative coilng part and chain stabilization in eukaryotes (not that they have orc instead of protein A). You have a done a great job, appreciate it very much Sir.

[@hussainbiology]

1.Yes Dna Polymerase Alpha has built-in primase in Eukaryotes PRIM1 and PRIM2. 2.Ur 2nd statement is right. 3rd statement is complex one and i need to see it how it drives... Btw thanks for appreciation..Glad to know that it helps...😊😊😊

[@yauheni7429]

@@hussainbiology Thanks again, for replying, keep up the good work Sir, much appreciated.

[@bidishakumari7095]

Explanation is brilliant but video quality should be more good, it is coming blur in screenshot

[@hussainbiology]

thanks for appreciation....Plz Play Video at 720 or 1080 and then take screenshot

[@pragyasharma3412]

Sir your videos are awsome 😍 In your 7-8 min. Video.. You gave a lot of information about the particular topic.. But i want to ask you something.. Now I'm preparing for gate life science... So this information is enough for gate?

[@hussainbiology]

first of all thanks for appreciation.... And yes this is enough information for GATE , CSIR or any other Advanced Biology exams

[@pragyasharma3412]

Thankuu soo much sir... 🙌

[@arundhatikarmakar6258]

E (eta) polymerase synthesises leading strand, d (delta) polymerases synthesises lagging strand. mentioned in watson's molecular biology of the gene.

[@doctornorthern]

It's Epsilon not eta ....🎉

[@maryamfarooq9452]

​@@doctornorthern😂

[@michaeleisenberg7867]

Shabir, Is it known how and when the Mg++ ion is loaded into the polymerase active site, please?

[@qaisarsbiology5267]

thank you so much dear for making this so simple while covering all the proteins and enzyme. You didn't mention about DnaT, PriA,PriB and Pri C which make Primosome complex with DnaB protein for loadin it, Please guide me to make it clear.....

[@havzaimtiaz3049]

But it is said that epsilon rep.the leading strand and delta replicate the lagging strand....?

[@hussainbiology]

That is according to old research...Plz refer NCBI for new research

[@havzaimtiaz3049]

@@hussainbiology thank u soo mch

[@hussainbiology]

@@havzaimtiaz3049 You are welcome.. keep sharing and supporting

[@alighteningstar0775]

Great work ♥️

[@hussainbiology]

thanks for appreciation

[@alighteningstar0775]

@@hussainbiology May Allah bless you ❤️

[@snowlinvinnarasij5352]

Sir, Is this notes sufficient for the preparation for CSIR NET exam?

[@hussainbiology]

Yes....this is sufficient

[@sumitthakur698]

All dobut clear in one video

[@hussainbiology]

thanks Sumit for appreciation..

[@reallifestories3687]

well done👍👍

[@hussainbiology]

Thanks Aliza for appreciation. Glad to know that it helps. Keep sharing and supporting

[@Catalinamercado0]

is E. coli dna replication and prokaryote dna replication the same?

[@Saurabh-uh8bg]

Yeahh

[@riteshsinghal1469]

Can u tell us the source. From which prepared this

[@bhatjas1820]

sir.. thanks for ur efforts.. and i have a query.. sir i have read that delta replicates lagging stranf and episilon replicates leading strand.. plz do correct me

[@biology_mcqs3037]

U r having old concept

[@biology_mcqs3037]

Check out ncbi website

[@hussainbiology]

www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/ Recent Studies , this is the summary of study : The major findings of this study and their implications are summarized below. (1) Our observations indicating the prevalence of L612M Polδ generated signature mutations on both the DNA strands in pol3-L612M msh2Δ at different positions in the genome in two different S. cerevisiae strains provide positive proof for the conclusion that Polδ replicates both the leading and lagging DNA strands. (2) In agreement with the role of Polδ in the replication of both DNA strands, genetic analyses with the pol2-M644G Polε mutant lacking MMR have failed to provide any evidence for the involvement of Polε in the synthesis of the leading strand. (3) We provide evidence that in addition to MMR, Polε exonuclease and Exo1 function in the removal of Polδ replication errors from the two DNA strands, and that these different mismatch removal processes can act differentially on the leading and lagging DNA strands. We suggest that yeast strains differ in the relative contributions of different mismatch removal processes for correcting Polδ errors from the two DNA strands. (4) Previously, it was concluded that in S. pombe, Polδ and Polε replicate the lagging and leading strands, respectively. A reconsideration of published data, however, implicates a role of Polδ in the replication of both the leading and lagging DNA strands in S. pombe also.

[@hussainbiology]

Most of the books have mentioned what you are saying but 2015 recent study has shown what i have mentioned. www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/

[@bhatjas1820]

@@hussainbiology thanku sir

[@ashasrivastava9222]

You are such a gem😍

[@hussainbiology]

thanks Asha for appreciation

[@sumoqil8601]

Hi sir may I know for DNA repair, the enzyme that involved in proofreading is DNA polymerase III for prokaryotes right??? Then the enzyme used to replace the damaged nucleotides is DNA polymerase 2?? Or both proofreading and replacing the nucleotides are done by DNA polymerase 2 ????

[@appannakhot6622]

Polymerase 3 has exonuclease activity once it completes the replication from 5 to 3 prime then it go for 3 to 5 prime exonuclease activity(proofreading ). After this in case still any wrong nucleotides are present on dna strand then DNA polymerase 1 will come and repair it. Dna polymerase 2 also involve in proofreading activity or dna repaing activity but correct function is still unknown.

[@ankitasamal8067]

Thank you so much ❤

[@hussainbiology]

Most welcome...✌️

[@ranjeshsharma6942]

In DNA polymerase III ( prokaryotes) there Should be 5'-3' instead of 3'-5'.

[@hussainbiology]

Yes..

[@squirrel670]

yay i need this so helpful

[@hussainbiology]

✌️✌️✌️

[@swagatmallick209]

Thank you so much sir

[@hussainbiology]

most welcome

[@akankshabhardwaj2877]

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[@hussainbiology]

thanks Akanksha for appreciation.❤️

[@dr.muzafarhussain9821]

Super.......

[@hussainbiology]

Thanks for appreciation

[@rezurizwan3512]

Hats off sir

[@hussainbiology]

Thanks Rezu for appreciation..

[@midaloshakantsieni2882]

You are the best

[@urmibarmudai1739]

Thank you

[@hussainbiology]

You are welcome.. Keep sharing and supporting ✌️

[@bratatidas4647]

Dna polymerase enzyme always acts from 5'end to 3' end... Sir pls let me know why u hv mentioned 3'-> 5'.. 🙂☺️

[@hussainbiology]

DNA polymerase moves along the template strand in a 3'-5' direction, and the daughter strand is formed in a 5'-3' direction.

[@misbashabir2063]

Thank you sooooooooooo much sir

[@hussainbiology]

thanks Misbah for appreciation

[@xaaboopinkly]

Thanks sir!

[@tayyabsulehri439]

Good

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R u from kshmir

[@hussainbiology]

Yes Sir...

[@WasimAkram-tk9sh]

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[@hussainbiology]

Wasim bhai,,, i upload video on Mondays only which means i can upload only four videos before 15 December of which DNA replication videos are coming soon.

[@WasimAkram-tk9sh]

@@hussainbiology thanks to reply me ....

[@knowledgehub4276]

Can I have pdf if this ?

[@elisalcedo3993]

Thank you so much

[@hussainbiology]

Thanks Eli for appreciation...Glad to know that it helps

[@biopaths]

Can I get names of reference books

[@hussainbiology]

Lodish Molecular Biology and Bruce Albert's Molecular Biology

[@princevarshney6551]

Sir how can I contact you to join your channel

[@hussainbiology]

I think there is a Join button available there....???

[@merve_n]

God bless you

[@shabanasyed369]

Thanq sir

[@hussainbiology]

You are welcome Shabana.... Keep sharing and supporting

[@blockchainguru1379]

Great

[@hussainbiology]

Thanks buddy for appreciation..

[@hinahina7779]

Sir ap bht speed may btata hai plz thora sa slow prhya kra

[@hussainbiology]

Theek hai... InshaAllah will try my best

[@hinahina7779]

Thnku sir

[@hinahina7779]

Sir ak or bt k ap bht hi small writing my leikhta hai thora sa bold kia kra

[@hussainbiology]

Improving aur haan My Videos are to be watched at 480p or 720p resolution where even smallest letter can be recognised. Try it

[@hinahina7779]

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[@bidhisingh9982]

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[@hussainbiology]

thanks Bidhi for appreciation...

[@Hyumifu]

Thank you 😭😭😭❤️

[@hussainbiology]

Most welcome.. all good?

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Well explained, but your teaching speed fast

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[@hussainbiology]

Thanks Anjali for appreciation....and i wil surely be slow in future videos

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Thank u

[@hussainbiology]

You are welcome....

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is there an even more dumbed down version for me haha I just need to graduate :(

[@Thewiseenigma]

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[@Shairi_aesthetics3930]

Plzz slow sirr........😑

[@hussainbiology]

Adhez Jinab.... Apologies

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[@hussainbiology]

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THANKS 🤍

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[@anjalimishra1154]

Thank you so much ❤️

[@hussainbiology]

Most welcome ✌️✌️