DNA Replication in Prokaryotes | Initiation

In this video we have discussed the Initiation part of DNA replication in Prokaryotes where the enzymes and proteins attach to the DAR sequence and initiate the process of unwinding . The unwinding is done by Dna A protein and then other proteins accompany the initiation process.
The Proteins involved in process are :
Dna A protein : ADP and ATP Bound.
Dna B : The Helicase enzyme which breaks the Hydrogen bonds.
Dna C : Helicase Loader.
Dna G : Primase enzyme.
Previous Video : • DNA Replication | Prok...

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Wow sir u r an artist who explain it's knowledge in terms of art. Tysm for explaining this topic

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[@Emilia-we2dc]

Literally the BEST video on this subject. Summarized everything that was in the textbook way more visually. Thank you!!!!

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thanks for appreciation....Glad it helps

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You can also check here. kzbin.info/www/bejne/sKCtf2CGi8mYp8U

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Which book?

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Which book?? Please do tell

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Thank you very much Hussain Biology

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thanks for appreciation..Glad it helps✌️

[@Mission-Omega.]

You are the boss of biochemistry,Sir.♥

[@hussainbiology]

thanks for appreciation

[@RohitPant04]

Great explanation, Hussain but doesn't Ori-c sire in prokaryotes contain 9 nucleotide motifs of 5 repeats & not 4 repeats as you mention here?

[@alisalisna2019]

I agree with you! There is for sure 5 repeats of 9-mer motif. Plus at the end of his lecture, he mentioned a DNA pol III holoenzyme, that in fact has three DNA polymerases (not just two!): one of them is working on the leading strand and another two on lagging strand - "trombone model". Here is some evidence: pubmed.ncbi.nlm.nih.gov/22157955/ www.ncbi.nlm.nih.gov/pmc/articles/PMC2859602/ kzbin.info/www/bejne/qanIlXWIdpZriNE

[@kaavyasurianarayanan8247]

I agree with you!!

[@risingsun9247]

You are right there are 5 R boxes instead of 4

[@risharajkhowa5060]

Yes , theta are 5 9mer repeats

[@RobertJurilj]

No, there are 4 of 9mer repeats in E.coli He was right

[@ammaramehak9473]

Nice videos for post graduated students thanx

[@hussainbiology]

thanks Ammara for appreciation.... Glad to know that it helps

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Thanks u so much U make every concept very easy

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[@bilaliyafcjrusson2967]

⭐⭐⭐⭐⭐ God bless you sir and shine like a star in the sky of knowledge......

[@misbahnaik4987]

Excellent explanation

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@debaswapnaray3295]

But sir pehle dnaB bind karta he uske baad ssb protein nhi toh helicase kam nhi karega agar pehle ssb lagade toh...otherwise its a best explaination video

[@hussainbiology]

Thanks for ur feedback and that is right what u say . I too have mentioned the same @ 3:59 Thanks

[@kathy-leew3989]

My professor gave us a different approach as to how the dsDNA becomes ssDNA at the 13-mer region. They told us that the ssDNA-binding-domain in the DnaA Protein causes the strand separation and didn’t mention an introduction of topological stress due to the coiling of the DNA around the Protein DnaA. I find your explanation much more logical. Thank you!

[@muskangoyal4030]

sir there are 5 9mer sequences rather than 4...

[@hussainbiology]

yes.. accepted.. there is a site missing

[@laxmanhanji6301]

Simply supperb

[@snehaldesai4423]

There are 5 copies of 9 mer sequence

[@nifatjan7458]

Clear cut concepts....great explanation....thnx sir

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[@nigelsimbarashe6433]

i like this, pure accurate dispensing of molecular biology knowledge. Thank you very much Sir

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I am a Hussain Biology loyalist✊

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Glad to know that... Lots of love

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[@hussainbiology]

thanks Shweta for appreciation.... Glad u like my stuff

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yes.. they do... very much!!! great efforts👌

[@merve_n]

God bless you

[@biologytutelage6960]

The video is good... But i found it quite complex to understand.... It should be more simplified...

[@bidhisingh9982]

u r awesome my bro . thanks.....

[@vrindasingh1306]

Thank you so much sir, its really helpful to clear the concepts.

[@hussainbiology]

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[@hafizmuhammadrehan074]

Ahaaa kea bat ha🤗🤗🤗🤗

[@NaDee-mh2po]

I have a test for this on Thursday. Thank you for making it easier to understand.

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Thanks Daniel for appreciation...

[@takemein]

You can also check here. kzbin.info/www/bejne/sKCtf2CGi8mYp8U

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Perfect! Just what i needes :)

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Thank you sir,make more videos on CSIR topics

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[@lonenaveed2870]

Thankyou so much sir

[@nouriakhan8656]

MashAllah ...hats off to u .....brilliant lecture delivered ❤..... Teach pharmacology as well...😢💔 will be thankful to u 😊...

[@hussainbiology]

Thanks for appreciation.. Glad it helps.. InshaAllah Pharmacology soom

[@nouriakhan8656]

JazakAllah ...

[@barashakalita7772]

Does the four 9 mer sequence lies on the downstream of replication recognition sequence

[@laxmanhanji6301]

Supperb

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thanks for appreciation

[@WasimAkram-tk9sh]

hats up sir may allah blessed u sir

[@riyajiyani3844]

Are these videos enough to study for concept of CSIR NET JRF or GATE biotechnology

[@hussainbiology]

Yes definitively.....

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[@hussainbiology]

thanks brother... Yes i am Kashmiri

[@shivaniojha4344]

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[@TheZitface5]

5:05 I think you got your leading/lagging strand messed up

[@hussainbiology]

yes i have a mistake there....

[@Wanderer_priya]

9 mers seq are 5

[@dr.muzafarhussain9821]

Nice explanation....make more vedios..

[@hussainbiology]

Thanks for appreciation

[@anavictoriagonzalezcarreon252]

Excelent!

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thanks Ana for appreciation..

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👍👍👍👍

[@hussainbiology]

Shukriya boi...

[@alfredafriyieasiedu]

Very detailed and understandable, thank you!

[@saibarsha4614]

Thanx sir for solving my doubt through this vdo

[@hussainbiology]

Thanks for appreciation

[@mitynonang1372]

What does mers mean sir plz reply?????

[@hussainbiology]

Mer means part ( in Greek ).. like hexa-mer ( six part when we have Length of six oligonucleotides)

[@ammarahmehak9986]

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Ammarah I have already replied to you abt this , currently i am unable to upload videos due to unavailability of high speed internet.

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Alhamdulillah...I am really glad to know that..... All the best

[@kartikeyverma6651]

how does replication occur in bacteria during starved condition

[@kaavyasurianarayanan8247]

Woaahh!! You are sooo good sir!! Thank you so soo much!! Really helped me!!

[@hussainbiology]

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[@monishas906]

Tq sir pls more and vedios

[@sahibaandherideas2252]

9 mer sequence -5 times

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i use Lodish and Alberts cell biology

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[@tanu6464]

Thanku

[@armaansidhu3018]

Why there are four 9 mer sequence on ori? Why R3 is not mentioned in the diagram ?

[@hussainbiology]

apologies... certainly there is one ori site missing

[@anamikagarg9703]

This is so helpful, Thanks much for making this.

[@hussainbiology]

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Good.. 🤗

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[@nishanisharma6289]

👍Well explained Sir. Thank you so Muchhhhh

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What a nice explained sir

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[@hussainbiology]

thanks Purnima for appreciation

[@WasimAkram-tk9sh]

sir we r waiting 4ur next lectures elongation n termination process ...Insha -Allah it will b very soon

[@hussainbiology]

InshaAllah Elongation and termination process coming up on following Monday

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This video is so old but still it is the best for this topic. Excellent👏

[@hussainbiology]

Glad you think so!

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You're so welcome!

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Best detailed video on entire KZbin

[@jyotisoni9814]

Hey! Hope u will reply soon. I have my mol. Bio exam tomorrow and there is a topic written in my syllabus "structural genes (as +nt in mRNA) - existing gene number by kinetics of RNA driven reaction" I m not able understand what should i write on this and also not getting it on google.

[@hussainbiology]

Hi Jyoti , have read ur query but a also couldn't find anything related to it.

[@jyotisoni9814]

@@hussainbiology Okay. Is that structure gene ....mean gene structure? In reference to internal organisation what it could be.

[@hussainbiology]

@@jyotisoni9814 Most probably it could be the Structural genes organisation ( structural genes include lac z lac y and lac A genes -operan ) there are several other structural genes but these are the most imp ones

[@jyotisoni9814]

@@hussainbiology okay....tnq so much.

[@nithyapriya.n1047]

Very well explained sir...Thank you..

[@hussainbiology]

thanks for appreciation

[@Arrahaha]

The best you are.

[@hussainbiology]

thanks for appreciation

[@ridhazainab3225]

are u from kashmir

[@hussainbiology]

Yes Ridha I am Kashmiri ....

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Thaaaaaanks a lot

[@hussainbiology]

Most welcome ✌️

[@firdoskhan5580]

I want the link on DNA replication on the Goole site ..... Can u plz provide it.......

[@swarnapravajenajena2803]

Tqq sir. i need dna replication NOTE

[@hussainbiology]

I haven't compiled notes yet.... Thanks for appreciation

[@prakritisamnotra6671]

Thankyou so much for making this too easy

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@monishas906]

Sir pls help me for to qualify the csir net .

[@hussainbiology]

Study and do continue watching videos...

[@soundariyasoundararajan9133]

sir in starting you said from 5'-3' but when Dna G Protein binds why you said from 3'-5' can you please explain sir

[@quranbydaniyal]

Antiparallel

[@aashmagyawali6331]

Thank you so much for your effort.. clearly understood

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@Helpnote9987]

Does this process occurs from ori site of plasmid?

[@letstudy2425]

What is the function of DNA pol 1 ...if DNA poly 3directly coming after primase

[@girikowshik3724]

Very clear explanation sir

[@hussainbiology]

thanks for appreciation

[@satyasantan855]

हिंदी बोलो यार हमलोग भारत के है 😂

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Kya fayda itne mehenge textbooks kharidne ka

[@hussainbiology]

kyu

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nice bro....!! as usual..👍👍👍

[@hussainbiology]

Thanks Suriya,,,,

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[@hussainbiology]

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Where did you learn this bro.

[@hussainbiology]

from books and internet

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@@hussainbiology which book?

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👏sir plz... do upload more videos

[@hussainbiology]

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[@whats_on_your_mind_asksbhavika]

You explained it so well. Thanks

[@hussainbiology]

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Thanks Salman for appreciation

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Your graphics are spot on. 🔥

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will improve

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great

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Thanks for appreciation... Glad to know that it helps