Supercoiling is something I struggle to get my head around, so this was super helpful, thank you!
@MITxBio4 жыл бұрын
Thanks for the feedback! Try our courses, too! bit.ly/700xBio
@himanshuprasad44096 ай бұрын
Juji
@himanshuprasad44096 ай бұрын
Chutchattam
@himanshuprasad44096 ай бұрын
Hello sir
@himanshuprasad44096 ай бұрын
Boorchatta
@laeunlee25373 жыл бұрын
7:27 *video suddenly became horror
@nephartuzim88793 жыл бұрын
@Crosby Isaac Shut the fuck up dumb fucking bots
@swatimavinkurve23052 жыл бұрын
understood the whole concept of DNA topology in 7 minutes of this video than 7 hours of lecture. SO HELPFUL! THANK YOUU
@mohammedkarim86277 жыл бұрын
why is it so hard for me to understand such logical problem ?
@ibnabbas59573 жыл бұрын
thank you so much!!! your explanation was clear and easy to understand. My professors terrible and vague explanations was making me anxious and stressed - again thanks!!!
@MITxBio3 жыл бұрын
You're very welcome! Join our free-to-audit courses, too! bit.ly/700xBio
@hiranshreebabaria5 жыл бұрын
the way u guys explained using tubes was really helpful for visualization thank you very much for the video
@MITxBio5 жыл бұрын
Thanks! Try our 7.28.1x Molecular Biology course, too. Registration open now for a Nov. 6 start. bit.ly/2AC9zP7
@dmadhav48673 ай бұрын
Is it available now @@MITxBio
@sandhyatelluri23462 жыл бұрын
this is the best explained video I have watched so far about DNA supercoiling . Thank you so much.
@dr.angelbaez633 жыл бұрын
Thank you, when I understood the importance of each topoisomerase, I understood the whole process.
@himul95264 жыл бұрын
You have no idea how much I'm thankful you.
@MITxBio4 жыл бұрын
Glad it was helpful! Join our courses, too! bit.ly/700xBio
@shamithagovind74918 жыл бұрын
"Thanks for watching" haahaa. :P
@isupportcharliehebdo-moham30815 жыл бұрын
nobody in the world could explain dna supercoiling this better..thanks.
@MITxBio5 жыл бұрын
Thanks! Try our 7.28.1x Molecular Biology course, too. Registration open now for a Nov. 6 start. bit.ly/2AC9zP7
@charliebrett75103 жыл бұрын
could you please explain the writhe thing again? What circular strand in the right direction, which colour are you on about?
@internalmedicinedoctordoct50573 жыл бұрын
Try this kzbin.info/www/bejne/l3WZaJqfa8eLrdU
@user-ec6kt2fg7m4 жыл бұрын
One moment you're playing with threads the next you're learning topology of DNA with Knot theory. Every time now that I think of grandma knitting I think of knot theory.
@foodnuts41015 жыл бұрын
Thank you for the video 😭 I finally understood!
@pollydowdlepolly4 жыл бұрын
Still a bit confused but definitely understand more than I did when I started!
@321123Nelly4 жыл бұрын
Same
@jaivenkats89858 ай бұрын
A single thanks is not enough. Lots of love ❤❤❤❤
@erikvitalis31074 жыл бұрын
i am still confused af :D
@shiyaoli23704 жыл бұрын
Makes the topic so clear to understand! Thanks
@MITxBio4 жыл бұрын
Thanks! Join us in the course as well. bit.ly/MITx7281x
@timur22476 жыл бұрын
Thank you. This video helps me understand this section of genetics.
@MITxBio6 жыл бұрын
Excellent! Glad that you liked it. Try our free Introduction to Biology course (goo.gl/Y5QcRa) or Molecular Biology course (goo.gl/ZDMVza) starting in January to learn more!
@arnabdas58833 жыл бұрын
I'm just hugely grateful to you for making it so clear,I just banged my brain over this uncleared concept.
@chantalgillett77976 жыл бұрын
Extremely well done video. Thanks for the clear explanations.
thank-you for providing this easier version of DNA topology.
@nileenakd92532 жыл бұрын
Well explained, thanks
@michaella51104 жыл бұрын
This video was so helpful, thank you!
@luizinxz988 жыл бұрын
excelent video! thx for sharing!
@BD-rj8wb3 жыл бұрын
thx for sharing! thx for sharing! thx for sharing! thx for sharing! thx for sharing! thx for sharing!
@generator69468 ай бұрын
Fantastic! More puzzle pieces!
@bhaktizanzrukiya9 ай бұрын
Not gonna lie that was 7 hours of lecture in that 7 mins
@jakobvanspronsen91384 жыл бұрын
understood it by watchihg once, and my english is awefull. This video is excelent!
@akankshyadash82694 жыл бұрын
Great explaination
@chandnisingh87072 жыл бұрын
Thank you 🔥
@MITxBio2 жыл бұрын
You're welcome 😊
@saeedekaasi10226 ай бұрын
Thank you very much. It was really helpful.
@kelleyforeman7 жыл бұрын
thank you for this excellent video!
@akhilachandrans42443 жыл бұрын
Really helpfull. Finaly I got it.Thank u for this one..
@MITxBio3 жыл бұрын
Glad it helped! Join our free-to-audit courses, too! bit.ly/700xBio
@pujeetjha82652 жыл бұрын
so good!
@user-ky1kf3hu3v5 ай бұрын
Thanks!
@reenamohanta29945 жыл бұрын
Wow .....thanks for the demonstration ......... it cleared a lot of confusion .....🙂
@ritikabounthiyal64274 жыл бұрын
Thanks for the video...nice explanation
@MITxBio4 жыл бұрын
Glad you liked it!
@el13393 жыл бұрын
Superb... Plz make more videos... It would be helpful
@MITxBio3 жыл бұрын
Thanks! Also try our Intro Biology course! bit.ly/700xBio
@mayankrajsuman15534 жыл бұрын
wonderful video, it helped me a lot.
@MITxBio4 жыл бұрын
Glad it helped!
@sadiakhalid31094 жыл бұрын
Thanks ma'am
@akshayam97736 жыл бұрын
Is the course ready to start? it would be best if it does starts
@MITxBio5 жыл бұрын
Thanks for your interest. 7.28.1x Molecular Biology Part 1 starts next week. Registration open now for a Nov. 6 start. bit.ly/2AC9zP7
@teegee11-58 Жыл бұрын
Super helpful!
@asmita_19992 жыл бұрын
Thank you for this video ❤️
@drujjawalrathore5 жыл бұрын
Wow!that was just awesome
@MITxBio5 жыл бұрын
Thank you for the feedback! Try our courses as well. bit.ly/2AC9zP7
@BorisNVM10 ай бұрын
very cool video
@hussainal-daheri3134 Жыл бұрын
شكراً
@noahgrasser25572 жыл бұрын
great video thank you:)
@MITxBio2 жыл бұрын
Glad you liked it! Try our courses, too. bit.ly/700xBio
@biofisicoquimica64705 жыл бұрын
May I use your video for teaching? I would need to add Spanish subtitles. Thanks!
@MITxBio4 жыл бұрын
Sorry, we didn't see this post earlier. Yes, you can use the video for teaching. We can add Spanish subtitles if you can do the translation. Email mitxbiology@gmail.com if you are still interested.
@neginjson31474 жыл бұрын
It was really helpful thanks:-)
@marco15x155 жыл бұрын
I can't understand why in the overwound DNA the number of bp/turn decreases c.c I mean, when we use the ethidium bromide, the number of bp/turn increases and the Wr tends to become positive. Could someone explain me this? Thanks PS: The video is awesome
@annahall84805 жыл бұрын
When you add intercalators like ethidium bromide, the binding of the intercalator to the base pairs results in a local unwinding of the helix by about 26 degrees - a reduction in twist (and an increase in writhe). Picture it - as the strands uncoil from each other slightly, the twists in the strand get further apart, so there are more bp/turn. However, this is UNtwisting the DNA from its relaxed state. In overwound DNA, intercalators are not used to wind it up. More twists are added to the double helix. As the twists in the strand increase in frequency, the number of bp/turn gets smaller.
@akshithany68452 жыл бұрын
Very informative.. Thank you
@MITxBio2 жыл бұрын
Glad it was helpful!
@funkdefied16 жыл бұрын
Incredible
@agriculturalknowledge24906 жыл бұрын
thanks
@donger05 жыл бұрын
thank .... you....
@PlushieMistress6 жыл бұрын
Question : why would any organism use Type 2 topoisomerase when using type one twice yields the same result ? one consumes ATP and the other Doesn't
@samiali-gn1go6 жыл бұрын
When you have cell replication, in prokaryotes, you get two dna strand which are circular and connected with each-other thus when you want to separate the two cell dna circles from each-other you have to use the type two to losen up one of the helixes and take those two blunt ends to the other side, and from there each cell in the cell replication get a dna strand each. Otherwise you would have two interlocked dna circles. Do you understand why now?
@PlushieMistress6 жыл бұрын
Why can't i cross over the strand one at a time with type one instead of two at the same time with type two? It even said in the video at 4:39 that crossing over one by one yields the same result .
@pushpankkatare42264 жыл бұрын
type two topoisomerase in bacteria helps in decreasing the linking number by two(refer Lehninger), but for eukaryotes type II topoisomerases cannot underwind DNA (introduce negative supercoils), but they can relax both positive and negative supercoils, I think what is told in the video is a bit misleading
@brishtibrishti92067 жыл бұрын
helped a loooot..thnx
@diegogranados27934 жыл бұрын
I still don't get it but still great video!
@kuldeeparya93922 жыл бұрын
After watching 20s to 30s of videos on thia topic... Finally!!!! I found someone who just explained this with so much ease and a dumb like me understood this 🥲 Thankyou so much mam for this explanation....you have saved me from failing in exam... thankyou so much again 🥲🥺
@MITxBio2 жыл бұрын
Also try our Introduction to Biology course! bit.ly/700xBio
@chunglamyu882 Жыл бұрын
what happen if simply increase the length of both strand? will it result more turns and more supercoiling?
@poorvivishwakarma79457 жыл бұрын
It was awesome mam..
@kiranjeetkaur35296 жыл бұрын
Thank you so much... now, finally i am able to understand DNA topology.... Best video...great...
@MITxBio6 жыл бұрын
Thank you for your feedback! Try our courses, too. goo.gl/KEbNJ6 goo.gl/TCRrJ3
@deliamariacoman2144 жыл бұрын
i still didnt get the idea of positive and negative
@siddhanthravichandran32454 жыл бұрын
Ok from what I understand, positive supercoiling is when u tighten the coils together such that there is more tension, so the double helix wraps around itself to relive this tension. helps. Negative supercoil is when u loosen the coil, but now that's it's loose it can wrap around another time, sort of like giving it extra space, I imagine this to be sort of like how earphones get coiled up in pockets, since they are in an unwound state, they are loose and can be pushed around easier causing coils. This can be thought of as a compensation mechanism, where the coil " tries " to become tighter. Hope this helps , although u probably already found ur answer by now lol
@sccm1004 жыл бұрын
4:34 isn't the top and the bottom the same shit? what difference does it make which strand goes on top?
@alminmekic44184 жыл бұрын
This is a way for me thinking of the difference and if the coilong is negative or positive. Put your thumb up like you would do when you wanna say "good job" for instance, do this with both of your hands. Picture your thumbs being the "ends" of the figures in the video, or the curves furthest away from the crossing point- your right hand shows the right end/curve, and your left hand showing the left end/curve. Then put your hands close to your desk and "rotate" your wrist forward so that your thumbs hit the desk. Your right thumb (as the right end) will rotate in the same way (forward) as positive supercoils. Your left hand (as the left end) will rotate in the same way (forward but the opposite end) as a negative supercoil. Most people ar right handed, and so am I, so I associate right with positive and left with negative.
@Jmjholden4 жыл бұрын
the direction of the helix changes RH to LH and their bookkeeping system labels those at +1 or -1. This is a net change of 2
@jorgeocampo6503 жыл бұрын
goog video
@Rajeshkumar-dd8zr6 жыл бұрын
♥️♥️♥️Very very thanks fr the vdo♥️♥️♥️♥️
@bubble_gum15338 ай бұрын
How did the writhe number increased by 2 by type 2 topoisomerase???
@sukanyaroyart8 ай бұрын
Initially, the dna was negatively supercoiled with a LEFT handed turn. So by convention, W=-1. Topoisomerase 2 cut both strands and passed the dna through the gap thus created. Now the dna became positively supercoiled with one RIGHT handed turn. So W=+1. So from -1 to +1, the value of W increased by 2.
@YuRiSunga3 жыл бұрын
Upload about the Central Dogma!
@MITxBio3 жыл бұрын
Thanks! There is more in our courses. Join our free-to-audit courses, too! bit.ly/700xBio
@vishwabarchha23756 жыл бұрын
The course mentioned is not available currently, is the course going to happen again?
@MITxBio6 жыл бұрын
Yes, thanks for your interest! It will start in November and stay open for a longer period of time. Sign up on the wait list on our site to get a notification when registration opens. web.mit.edu/mitxbio/courses.html
@MITxBio5 жыл бұрын
The course registration is now open. Join us! bit.ly/2AC9zP7
@pageread71354 жыл бұрын
Big FaN Love from india Please make subtitles in hindi
@magihiyoriyona_nanatsu50923 жыл бұрын
Can you please add the French subtitles? it's hard to concentrate translating your words and trying to understand
@workpual4251 Жыл бұрын
2:30 it's not underwound it wound more why you call it underwound?
@prateekyadav98116 жыл бұрын
Please tell me what you used for the DNA model?
@MITxBio5 жыл бұрын
Lab tubing that we painted red and blue on the inside.
@ayanmandal8345 жыл бұрын
Wow
@Sol-zf9wc3 жыл бұрын
6:24
@Jini11777 жыл бұрын
Great video!!! I was just wondering how does negatively supercoil DNA store energy in it's coil?
@footfault19414 жыл бұрын
While this is intriguing, evolutionists would find their job, hypothesizing a theory, further complicated! Great!
@YuRiSunga3 жыл бұрын
Add Filipino subtitles
@AweZombE7 жыл бұрын
Wait guys what does this have to do with topology of the DNA
@DudeWhoSaysDeez7 жыл бұрын
everything
@vloggerno13915 жыл бұрын
please upload translation
@MITxBio5 жыл бұрын
If you know someone who can do the translation (and tell us what language), we are happy to upload additional transcripts.
@francislemieux072 жыл бұрын
but why does't dna get tangled into a mess of knotsssssss
@sukanyaroyart8 ай бұрын
That's the requirement, that's how tgey are packaged and chromosomes are formed.
@gyanichele3 ай бұрын
Because there are topoisomerase which relaxes the DNA
@RoyMustang. Жыл бұрын
❤❤❤
@ouahibasabri1836 жыл бұрын
I don't understand scientific english very well any translation to french
@MITxBio5 жыл бұрын
If you know someone who can do the translation, we are happy to post additional subtitles.
@parthchauhan72875 жыл бұрын
Hi what is RNA ? How many types of RNA? The structure of RNA what is Amino Acids ? The structure of Amino acids ? What is Genes ? what is Chromosome? can you share information of list of systems of the Human body ?
@ronymararenhas9313 жыл бұрын
Cure HIV crispr cas 9.
@saramadeleinezinniker38 Жыл бұрын
chome emmer noni drus aber jäno
@boozscha992 жыл бұрын
fml
@wasek1526 жыл бұрын
talking like robots is not helpful. thanks
@rajashrimazumdar83904 жыл бұрын
The accent is so irritating.. Not clearly understand.. 👎👎👎
@internalmedicinedoctordoct50573 жыл бұрын
kzbin.info/www/bejne/l3WZaJqfa8eLrdU Try this to solve your problem. Give comment about the video