DNA supercoiling explained

DNA supercoiling explained - This lecture explains about the DNA supercoiling and topoisomerase. it also explains about the positive and negative DNA supercoiling and the role of DNA topoisomerase in changing the DNA supercoiling. Watch this video lecture to understand the following-
1. DNA supercoiling types and how supercoiling works
2. Twist and writhe of DNA supercoiling
3. Role of topoisomerase in DNA supercoiling
For more information, log on to-
www.shomusbiolo...
Get Shomu's Biology DVD set here-
www.shomusbiolo...
Download the study materials here-
shomusbiology.c...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in KZbin. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.co...
LinkedIn - / suman-bhattacharjee-2a...
KZbin- / thefunsuman
Thank you for watching the video lecture on DNA supercoiling and the role of topoisomerase in DNA supercoiling.

[@zarachowdary6533]

May Allah bless you with huge success and happiness ... ❤❤❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@raveenabiswas3333]

Thank so much whenever I am unable to understand by reading books I refer only your video...a huge thanx to you sir ....I am preparing for net exam...Thank you soo much sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@moinakhan2151]

I recently found your channel and immunology seems to be very difficult for me that's why I asked but now I have found your playlist thanks for telling Sir. Your way of explaining is easily understandable. Thanks again

[@shomusbiologyofficial]

+Moina Khan you're welcome

[@rosafarias1565]

You made this so easy to understand, unlike my professor who complicates things way more than he should!!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@mariamrahimi-t3e]

You're the best!!!! Thanks a lot!!!! I remember I had emailed you about a certain question like 4 years ago and you answered, I appreciated it a lot, and ever since i have always came back to your channel when it comes to biology!! I just started med school and your channel will help alot!! :)

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@poonamvijaypuriya8815]

One of the best video for such a tough topic.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Honeyaami]

sir, you explain it all in a simple way. easy to grasp.. really like your videos.. very much helpful... thanks a lot...

[@nabajitbharadwaj5304]

At 15:48, you wrote that it is positive supercoiling, how can it be +ve, if the Lk is decreased from 9 to 6, according to your discussion it should be -ve supercoiling. I didn't get it.

[@sayantandas4505]

Thankyou very much sir, I had doubt on super coiling but seeing your video everything clear. After seeing your video, It help me to present my presentation on this topic infront of my college teacher

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@Anjali-gl7fz]

When I have doubts my first preference is shomu's biology..... Thank you so much for clearing my doubts 😇

[@shomusbiologyofficial]

All the best

[@monikumari5303]

Sir here your tell us that linking difference = l- l0 but actually it is l0- l is the linking difference i think so... So the. Linking difference us +3 not -3 so it is positive supercoiling 🦹🤔

[@shreyamb6118]

Such a nice lecture ❤️ the concept is clear ... Thankyou sir .... I always watch your lectures when I have doubts .

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@sanjuktaghosh8802]

Thank you so much Shomuda. I had quiet a confusion regarding the writhing during under and overwinding. Your additional example of replication solved it.

[@김대빈-w5m]

I don't understand unfortunately around 12:23 you said DNA has a right-handed (clockwise) helix having a right-handed supercoil (clockwise). That is right-handed helix structured DNA + right turn causes positive writhe right? And you said around 19:33 "positive supercoiling = left-handed turn = clockwise direction". But why left-handed? shouldn't it be right-handed as you said before?

[@medstunts3168]

video was good but it would have been better if animation was available....i am unable to visualize the process still

[@sawma3714]

Another question - When a supercoil is formed as a result of double helix unwinding, topoisomerases fix that and remove the supercoil but the unwounded helix does not necessarily return to its initial helical state. Thus we get diffence in linking number. This is how i get an image of DNA supercoiling after watching the video. Is it correct?

[@withlovesanya]

Thankyou soo much you explained it soo easily I couldn't understand a word from book. You are of the best teachers out here.

[@KHUSHBOOKUMARI-hw4zm]

You made me understand this topic very easiest way,,, Thankyou sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@anmiann601]

Thank you sir..it is very hard to understand the notes provided from college that's why I watch your lucture....it's very easy to grasp the idea thank you so much sir

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@myimmortal9488]

?Right handed supercoiled is negative and left handed is postive in ccDNA why u said the opposite

[@ananyoghosh361]

Its writren in leninger...that right handed helix is positve

[@mgold9999]

As the writhing number (Wr) can be both pos and neg and both increase or decrease the twist number (Tw) as the linking number (Lk) does not change in ccc it's all a matter of the Lk when Wr is 0 which equals Tw and if it's overwound or underwound with respect to Lk naught which is the linking number of fully relaxed DNA under physiological conditions which is usually bp/10.5 and this is what is changed by the Topoisomerases where the Topoisomerase I changes one twist and Topoisomerase II changes two twists (by changing writhes), right? So if delta Lk (Lk minus Lk naught) is negative the DNA structure is negatively supercoiled and has fewer twists when Wr is 0 and if delta Lk is positively supercoiled it has more twists when Wr is 0, right?

[@RubyKumari-mz4co]

Sir plz provide animation vedio for the same...

[@supriyapathak1111]

Thank you so much sir for this wonderful explanation 🙏😇

[@shomusbiologyofficial]

Thank you

[@amitdhyani5156]

Easiest way of explaining. Shukriya sir👌✌️

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@SL-my4fg]

right hand-anti clock wise?hoowwww? i cant understand it.

[@akeel77]

Thanks so much, I have my exam tomorrow!

[@shomusbiologyofficial]

You're welcome

[@DpkTamta157]

Very very thanku so much sir❣️

[@shomusbiologyofficial]

You're welcome

[@Vlogdaily09]

Thanks for this lecture for better and easy understanding.....🙏👍👍

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@theresecaparas3377]

What do you think is the importance of the DNA to have a negative and a postive supercoiling?

[@kusumqureshi6493]

Sir u r excellent teacher... I understood very well

[@ammarahkapur2389]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@malik8718]

❤️Thankyou Sir.....

[@shomusbiologyofficial]

You're welcome

[@suchit05]

Love u sir❤️

[@shomusbiologyofficial]

Thank you

[@supritadash2535]

Thank u.. so much.. ur lectures always help me..

[@shomusbiologyofficial]

+Suprita Dash Glad you liked my lectures

[@bareeraarshad2387]

Thanks for such amazing concepts

[@shomusbiologyofficial]

You're welcome

[@bareeraarshad2387]

@@shomusbiologyofficial Respected sir! Can you plz tell your qualification

[@meenublossom6860]

A big thank you... 😊 For explaining supercoiling in such a relexed n easy way 🙏🙏🙏👌👌Awsome lecture ✌️✌️👏👌

[@akramjahangirkhan1902]

Great , your videos are very useful for me , thank you so much 🙏

[@shomusbiologyofficial]

You're welcome

[@pushpankkatare4226]

bhai bahut aacha samzhaya hai maza aa gaya samazh ke

[@shomusbiologyofficial]

You're welcome

[@sudebahar8778]

Thank you so much great explanation and teaching

[@icsebiology6323]

Thanks for such a fantastic video 🙏

[@shomusbiologyofficial]

You're welcome

[@sravanigarimella167]

you are explaining things very clear.....but my doubt is clockwise turn of double helix produces negative supercoils ....//// positive ...plz clarify that doubt....and clear many studenyts confusion. (becoz it is useful for many students)

[@prabhleenkaur7756]

I think it produces negative supercoil.

[@aat85]

You are amazing 🤗👏👏👏👏👏 thank you

[@shomusbiologyofficial]

Glad you liked my lectures

[@aat85]

Your lecture help me with those biology classes and you teach very well 🤗👏 thank you for all the work that you do.

[@007-y1v]

acha dada bas inhi Ko reply 😊😊

[@surajmishra1125]

My professor - Shows confusing animations me - I have a better option ( Shomu Sir ) :))))

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@michelehernandez1290]

muchas gracias! me ayudo mucho

[@shomusbiologyofficial]

Thank you

[@reshmapremjith3239]

Superb class

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@chashabhusho]

Will you please explain how twist is decreased during separation of a closed circular DNA?

[@muhammadzuabi]

I think the LK should be 10 according to the drawing and the definition. Did I miss something?

[@oscarniiottodarko4510]

me too i also think it should be 10

[@manasranjan700]

Absolutely ...

[@mirajmohammad1169]

Thank you sir...😊 Great to learn from you.

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@mmmpr2261]

I feel like...I owe you so much!

[@azrathaseen]

Amazing lecture learnt a lot !!!

[@shomusbiologyofficial]

+Azra Thaseen glad to hear that

[@manibharathiduraisamy1344]

Sir nice lecture

[@shomusbiologyofficial]

Thank you

[@Debopriya-q1x]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@rezanur356]

it was a wonderful explanation

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@lakshmiparvathy5826]

Can't thank u enough❤️

[@shomusbiologyofficial]

Don't thank just like and share

[@payelmaitybiswas3165]

Sir, please make a vedio on Selfish DNA

[@AakashKumar-ue4xe]

Sir... right handed supercoil or clock wise is negative.. nd the anticlock wise is positive...

[@dreamercreations6753]

😍😍😍😍Super Definition Anna... Vera Another level of Explaination 👏👏👏👏👏👏👏👏👏👏👏👏👏

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures.

[@Manikalens]

Sir it was really helpful... Thankuu

[@emirsbio-chemistry4017]

Hello sir I need ur help..Meri Outline me 2 question hai DNA supercoiling se related 1 tu apky lecture se cover ho gaya hai that explain DNA supercoiling and second one is how DNA supercoiling Works in Flow And Transport Of genetic information..Can you plez suggest me What points need to be mention in this Topic..plez help me sir..

[@ayeshaashraf1996]

super amazing 💓

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Curious_Human954]

What a great of teaching, thank you so much, Sir!

[@anegg8571]

@Eye need Dinero U mad 😂

[@thurayae]

simplifying the complicated ideas as usual

[@SabyasachiChatterjee]

Can you plz make a video on EST ?

[@Bio24078]

Sir,as u told topo 2 is cleaved both strand of DNA. can u confirm me cleaved at same point of DNA.

[@vijaychauhan-po2jq]

SIR, LK number = number (couple) of nucleotide attaches by phosphodiesterase bond. right ?

[@alyaaalmery7939]

Great video thank you very much 😁

[@tarinibehera9985]

Sir plz provide the pdf

[@abhishekghosh8086]

Watson molecular biology summary

[@anegg8571]

Sir prefer Hindi rather than English. Anyways great effort sir

[@shomusbiologyofficial]

Thank you

[@soumyarupchowdhury7964]

pls make a video on DBT-NET also

[@shomusbiologyofficial]

+Soumyarup Chowdhury will do that soon.

[@sabbamussadiq9818]

Sir, supercoiling is present in eukaryotes or prokaryote or both.

[@shomusbiologyofficial]

Yes but mostly observed during the prokaryotic dna replication

[@sabbamussadiq9818]

In humans...is supercoiling present ...but the DNA is linear in eukaryotes...how will writhe be made....?? On one side of the loop , like the alphabet ' p'.

[@nadimahrahimii6368]

@@sabbamussadiq9818 yes actually in long linear dna which is not wrapped around protein this concept of linking number is not present and also writhing is not but in those kind of situation that just just like in eukaryotic cell when just one small part of double helix is needed so that part releases from histone protein now in this part of linear dna supercoiling can occur ! Why ? Because both side of linear dna wrapped around protein histone and cant round freely and can't broken the writing.

[@pijushsingha9232]

I don't know how to say thank u to u

[@shomusbiologyofficial]

You're welcome

[@moinakhan2151]

Sir can you please explain about immunology

[@shomusbiologyofficial]

+Moina Khan it's already done. A whole Playlist is made on immunology

[@niyasahamed9552]

thanks for this clip bro

[@shomusbiologyofficial]

+Niyas ahamed you're welcome

[@kajalthakur827]

Thank you very very much sir 🙏

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@Celtic_a]

Superr

[@kanikshagarg1728]

I didn't understand the concept .. That .. Positive supercoiling... Left handed show clockwise turn Right handed anticlockwise .. Verry confusing.. 😫😰

[@greeshmakichu2197]

thnku sir

[@shomusbiologyofficial]

You're welcome

[@istiakrana8969]

❤️

[@shomusbiologyofficial]

Thank you

[@faizahuq4562]

Why the sound of your video is so low

[@shomusbiologyofficial]

It was the voice. A decent one. Now the noise of the world is so increased!

[@kazisamiulislam9785]

animated video would be great

[@shomusbiologyofficial]

+kazi samiul islam yes. I will upload one of that

[@kazisamiulislam9785]

I love to watch your video...☺

[@omithhasan3775]

❣️

[@thefalcon3814]

It is " writhe " ..... not ride , right or wright .....

[@shomusbiologyofficial]

I said writhe only

[@nothing....5144]

😀😀👍👍👌

[@shomusbiologyofficial]

You're welcome

[@ath8859]

thanks respected sir .. I want share few problems regarding biochemistry .plz send ur email.id plzzz.

[@mahakehail8425]

Every exams on my life university, i remember your lectures to solve the qustions And you know what? I'm studying master degree now from your videos to understand again and again😁, So thank you very much! Finally i hope to upload more vidoes in different topics of science, cuz we are depend on your lecture videos Really thankk u❤

[@swetathakur7227]

Thank you so much sir

[@ashiskumarhial4083]

Thank u sir

[@shomusbiologyofficial]

You're welcome

[@chinmayabehera6315]

Thank you sir.

[@noyiseMagagula]

Thank you so much Sir for your content, even after 6 years it has helped a lot. Honestly I was lazy to learn this topic but after I went through your video I got the stepping stone I needed

[@shomusbiologyofficial]

All the best

[@aimalkhan5006]

thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@masterjiraya1118]

Thanku so much sir I was totally confused in books it is written that lk. Is constant but in example it is changing but u cleared all my doubts

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@sangeethakrishnan6431]

👍👍👍

[@shomusbiologyofficial]

You're welcome

[@maryum7192]

First lecture and i am impressed just wao... U are Amazing Man❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@aarthir2447]

Awesome explanation sir.... Tq 🙏❤

[@shomusbiologyofficial]

You're welcome

[@gunjanpatidar8403]

sir amazing I didn't know about this concept before watching your lecture thankyou sir for clear my doubts

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@fatezurislam3936]

Thank you so much 🫂

[@shomusbiologyofficial]

You're welcome

[@pijushsingha9232]

Bro ur awesome

[@shomusbiologyofficial]

Thank you

[@annymus9895]

Sir, ur voice is too low for out door...plz increase your voice quality..

[@shomusbiologyofficial]

+Anonymous I am trying to improve it soon.

[@harishkumarbio]

Increase your device volume... And use headphones...

[@your2321]

Thanks alot

[@shomusbiologyofficial]

You're welcome