Don’t Waste Leftover Petris! How to Save Them (Eco-Mycology Series)

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Hello Mycophiles, in today's video I show you all an experiment that has been in the works over the last year. I have been saving any leftover petris I may have from a pour, to see if I can use them later instead of throwing them away. Was it a success?
I hope you all have a great day or night!
🍄💜
Sage
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[@Mycophilia]

Fruiting content (free! But sign up for a free trial and get videos a month early) and Genetics: patreon . com / mycophilia

[@AndreaDingbatt]

@Mycophilia Im not great with Wrechnology ,(So, please excuse what might seem like a daft question,,,) What is Patreon?! Of course Ive seen it all over KZbin,,, but I Honestly havent a clue what its about!! Namaste,,,/\,, Thank you. Andrea and Critters. ...XxX...

[@bigtime4794]

Sage do you know if PE should always have casing layer? When to apply it? And best mix?

[@Mycophilia]

@@AndreaDingbatt Patreon is a place where you can support and follow content producers you enjoy with a monthly subscription fee. I have a few plans on there with varying benefits depending on tier, anything from genetics, access to a special discord channel where I will read every comment, and early access to fruiting videos.

[@Mycophilia]

@@bigtime4794 It doesn’t need one, although it can help prevent blobs early on. Check out my casing layer videos, I have a bunch.

[@bigtime4794]

@@Mycophilia yeah I did watch. Thanks.

[@MrJakutza]

Hey Sage, two weeks ago during your stream I asked for your blessing as I was about to spawn to bulk for the first time. Lo and behold today I got my first pins! Your videos greatly increased my chance of success, thank you for all the work you are doing !

[@Mycophilia]

Congratulations and thank you!

[@kevlarkarma]

Did anyone else think Petris was a game which involved tessellating bacteria or domestic animals? The title "Don’t Waste Leftover Petris! How to Save Them" I thought it was a game guide. Awesome content as ever.

[@MJ-gu6uu]

I had one sleeve with one cracked dish in it after delivery, and fragments of the dish pierced 3 cuts in the sleeve (~ 5-8mm long). I used other dishes without any sanitation (just like regular sleeve) for my transfers and had zero contamination 😄

[@AndreaDingbatt]

Thank you!! Im very Eco-friendly, and ,now I will be able to be even more so, when Im able to get started!! And, I also wanted to thank you again, for the Awesome and Honest journey that you took us on, at "Sages Intergalactic Nightclub," Also,if you are ever in the UK, you can be assured of a warm welcome, (despite the damp,cold and drizzly weather!!) Peace , love, Light and Laughter to you Sage! Namaste,,/\,,, Andrea and Critters. ...XxX....

[@Mycophilia]

Thank you Andrea 💜

[@peacesign.]

👍✌️good vibes to ya, thanks for the work as always. I had a friend named Saige with the "i" growing up. So yeah......cool 😂

[@Dirt_Worm]

If you do unmodded lids you dont even need aluminum foil for them. Foil does help with contam though.

[@madammycol_OG]

Thanks brother

[@tonystewart7624]

It's crazy brother it didn't send me a notification about this one. Either way I'm glad I found it. Thank you

[@Mycophilia]

:( I was wondering where you were at! Glad you were able to find it though!

[@Rolandfart]

I have been able to get multiple uses out of my used single used petris by washing them with soapy water spaying the inside and outside with iso and h202. I close them up and let them dry in my sab. Then I pour them like their new and I have only had a handful of failures.

[@enigma1247]

I do not like short vids! Lol. long form videos are always better. Sage has so much good info the longer the video the better. Screw anyone that prefers short form vids.

[@seekingsomethingshamanic]

This inspired me, ill do a video later to talk about what to do with old plates. :)

[@aidennymes6335]

I even reuse my petri dishes. They go through a multi step washing & UV disinfecting stage. they are just like new after that.

[@pheresy1367]

That is awesome! I will purchase a proper UV light... If you have any suggestions about getting good "bang for the buck", I'd love to hear them. Thx... :-)

[@aidennymes6335]

@@pheresy1367 I'ts all DIY. read up on UV-C safety (ozone)

[@theanalymous]

I use flat jars or polyethylene mini tupperwares so I can PC them, it's more practical

[@Mycophilia]

You can pour far more with a sleeve of petris than you can make prepours, plus you have full visibility + control condensation. You also do not need to clean them after you’re done. There are pros and cons to each.

[@InconspicuousOwl]

Glass plates are the way

[@Mycophilia]

PLASTIC! 😊

[@InconspicuousOwl]

@@Mycophilia eeeee xD fun fact: did you know humans eat a credit card or 5g worth of plastic every week D;

[@CorteX1988]

They're great for a photo contest but not practical for mycology imo. Having to sterilize them every time and lifting up stacks to pour sucks when using glass. Not worth it when I can get a case of 480pcs for 57$.

[@Lemonz1989]

I have glass plates that I wash with soap after use. I mostly use these. But I also reuse my single use plastic plates, when I do use them. I also just wash them with soap and store them in the sleeve they came in when I bought them. I don’t bother sterilizing any of them. I have a pretty unconventional way to pour plates. I leave them open while they cool in my still-air-box, so I will have less condensation. When they are cool, I close and seal them. I have a strong UV-C light in my box that is turned on while they’re cooling, so I don’t have to worry about the plates being sterile. The light kills all contaminants anyway, while the plates are cooling. I’ve never had a contaminated plate since I started doing it this way. I leave the room while the light is on, because it’s dangerous to the eyes and skin.

[@droidnick]

Very cool. Where'd you get the light?

[@MuchoNoBueno]

Just a quick ecological way, I digress

[@Mycophilia]

😉

[@cosput]

I'd love to know how to not throw away so many gloves, oven bags and foil.

[@CorteX1988]

I don't use gloves at all. As long as your technique is good it should be perfectly fine. As for foil, I've recently just been using a whole sheet and laying it on top of my jars while PCing instead of doing them one by one. This prevents any water from landing on the jars and you can just re-use it again as much as you want. This is assuming you have good lids of course.

[@wickedjack1222]

This is why I use glass petri dishes, I thought this was going to be about old grown out mycelium plates. Hell I've used 1 year old agar samples out of the fridge. Does the mycelium ever love getting outa that plate after that long lol very very vigorous !

[@Mycophilia]

Personally not a fan of glass petris, reusable petris in general

[@RawTubeTV]

What is your preferred agar recipe? Love the eco tips!

[@Mycophilia]

Regular old MEA! 5-10 grams malt extract, plus 10g agar agar

[@bravedove5041]

*I'm using glass petri dishes and sterilize them* 😉

[@droidnick]

I though HOCL was mycelium safe?

[@docphillips3291]

🍄🍄🍄

[@paulofx408]

What about using ozone generator to re sterilize them?

[@Mycophilia]

I would never recommend anyone use those in the hobby

[@paulofx408]

@@Mycophilia why? Isn't it safe or effective?

[@Mycophilia]

@@paulofx408 they are neither that, that is why

[@CMZneu]

...or you know use glass petris... tbh i used to do this but it's a pain, by drying you expose them to contam, when washing plastic gets more and more scratched and then you get more contam, etc

[@Mycophilia]

Watch the video and you will see this video is not about reusing petris 😂

[@AndreaDingbatt]

@@Mycophilia 🥰💞💕💜

[@urbanyeti659]

​@Mycophilia LOL. This is gold. The response and the video. Thanks Sage.

[@CMZneu]

@@Mycophilia Ohh you meant the ones left over from the stack, i just leave then on the table with the bag folded under them and use them next time, never thought about washing them and haven't had any problems with that.

[@LoLoThePirate777]

🍄❤🍄❤️🍄❤️💯👍🏻👍🏻👍🏻

[@spacepirate1543]

3 words glass petri dishes

[@Mycophilia]

Not worth it imo, disposable are cheap, no cleaning time involved, no need to sterilize, and doesn’t break. The only downside is environmental unfriendliness, but other than that it wins on everything.

[@spacepirate1543]

@@Mycophilia alright but i have a fleet 80 strong. they never fail and only have to buy more when i break 10. disposables are cheap in the short term but in the end i will spend less money and have less contam

[@Mycophilia]

@@spacepirate1543 idk, you also have to clean them, larger so more of a PITA to stack, plus they’re not any more sterile than disposables (assuming they’re sterilized correctly in the first place, whereas with disposables you don’t need to worry about that).

[@spacepirate1543]

@@Mycophilia you wash them in the dish washer and put them in a grow bag , fold the bag up at the top and remove all air. tape the bag with the blue painters tape it will go through the pc unscathed just chuck them in the cooker with the agar and pull them out in the sab (i have a flow hood so i do that) the staking is the hardest part dude just give it a try also works with Polypropylene ones micropose just started selling that don't break and could theoretically last a life time. but i like glass because it keeps the agar from drying out and can be used for long term storage if properly sealed like a year if you pour them thick enough

[@spacepirate1543]

and i guarantee that il have less issues with sterilization doing it this way

[@johnbauby6612]

I was very disappointed when I realized how much plastic waste goes into mushroom cultivation when mushrooms are so beneficial to the environment. Humans can screw everything up. That's what happens when we try to bend nature to our needs,