What is Gel Electrophoresis | Don't Memorise

[@unicorntae]

This is by far the best explaination of gel electrophoresis I've come across. thanks a million

[@Mahimahi-rn2ol]

Hey ! how do we separate cloning vector and recognition site that we cut from vector for introducing desired gene My question is are cloning vector also extracted through gel electrophoresis

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@@Mahimahi-rn2ol what is a cloning vector from your point of view?

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Me too

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Agree!

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The beauty of this channel is that they answer literally every question that will arise in your head about the topic ❤️

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Hey , I'm really thankful to you , because u r really helping students in understanding things, and I know there is lot of hardwork behind these animated vedios.God bless you🤲🙏🏻

[@InfinityLearn_NEET]

Thank you so much Angel for your appreciation. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. We love our Pi Army and would like to stay connected. 🤗🤗

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Neet

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I can't thank you enough for such a clear explanation of this. You literally helped relieve my last minute practical exam preparation stress. Thank you so much.

[@HyPERBOI._.07]

Best explanation for gel electrophoresis I've come across. simple and to the point explanation of the topic . Thank u a lot for this...❤️

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This made all the things clear so simply!!!

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Ya.

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[@franceraymundo3786]

Raymundo, Francezka J. •Gel Electrophoresis is a separation technique which separates DNA fragments based on their Length or Size. •The movement of charged molecules is exposed in electrical field and it occurs in gel medium, that's why it's called Gel electrophoresis. •To observe the DNA molecule, agarose gel is used with ethidium bromide (ethidium bromide is used beacuse when the agarose gel containing the DNA is observed under ultraviolet light, bright color bands are clearly seen). •Gel electrophoresis is considered to be one of the important techniques in recombinant DNA tech.

[@mariyammaturaka5184]

God bless you mam

[@chinmayeethakur5223]

Kudos ..this is just so to the point ..I am brushing my Biotech syllabus , that I have studied 8 years back ..and this helped me really

[@layaldergham45]

oh my gosh. this channel is literally my new go to for genetics, I love it

[@Nancy-pg9zu]

Gel Electrophoresis was a puzzle for me until I found your video.. Thank you.. For such a great animation.. ❤

[@ijazulhaq9202]

the most easy and best explanation i have ever heard in my whole eduactional exposure love u maam

[@saaduddin4504]

It's excellent.... Clearly explained hidden Words... Like Agarose which is given from Seed... Or DNA got Position charge because of phosphate ❤️❤️❤️❤️ Love From Pakistan 🇵🇰

[@abubakras-siddiq1694]

Pakistan Zindabad!

[@suhaniverma2010]

I m really..happy...coz i searched the whole internet...but couldnt find the video with this kind of explanation... Ik few points r also there which r not mentioned here...but overall the video is the best...100/100

[@tinashejohnjoni7765]

thank you very much for the best explanation of gel electrophoresis

[@nidhishreeg6292]

Thank you so much for teaching this in such an easy and understanding way!!

[@barkatullah9720]

You helped me much in physics to understand different topics and you have helped me in bio also. YOU ARE ONE OF MY BEST TEACHERS. GOD BLESS YOU ALWAYS...😍😍

[@riiel21]

Tiongco, Gabriel Felix D. 8-Edison Gel Electrophoresis • It is considered as one of the most important techniques in rDNA Technology. • It is a separation technique which separates DNA fragments based on their length or size. • It is based on the movement of charged molecules when exposed to an electrical field (occurs in a gel medium). • DNA is negatively charged. It is due to phosphate groups which are present in the backbone of the DNA's double helix. • The gel generally used for DNA is agarose gel which are obtained from seaweeds. • Treating ethidium bromide to the agarose gel makes it possible to see the separated DNA molecules because ethidium bromide easily binds to the DNA molecules. • The DNA ladder is a standard chart used to compare the bands obtained to easily identify the desired DNA. After obtaining the desired DNA, we can cut and extract the DNA fragment from the agarose gel which is a process called elution.

[@ayurgreenpalasa785]

Tell me the formula and structure of ethidium bromide

[@brendanliamrebote699]

Rebote, Brendan Liam P. 8-Avogadro Gel Electrophoresis • used to separate the different DNA fragments based on their sizes • movements of charged molecules when exposed in an electrical field and this movement occurs in an gel medium, hence gel electrophoresis. Process • DNA negatively charged (Due to phosphate groups) • Phosphate groups are present in the backbone of the double helix. • Agarose gel is usually used for DNA • The charge across the gel helps the mobility of the DNA fragments. • Not all fragments move at the same pace. (smaller fragments move ahead, bigger fragments find it difficult to move) Procedure • Requirements for gel electrophoresis - DNA sample - Gel - Casting tray - Comb - Electrical Supply • The wells are formed from the negative charge so that the DNA will go from negative charge to positive charge. • A colour loaded dye is used to track the movement of the DNA samples. •Buffer is used for better conductivity of electricity. • The loaded dye is much faster than the DNA samples because it must reach the anode first. If the loaded dye reaches the anode, it must mean that the DNA samples reached somewhere near. • To observe the DNA samples, we treat the agarose gel with ethidium bromide solution. • Ethitidum bromide is used because it easily bonds with the DNA molecules • DNA bonds can be seen under a UV light • DNA ladder is used to compare the size • Cutting and extraction of DNA fragment from the agarose gel is called Elution. •

[@AngelicaMayDSalvatierra]

Angelica May D. Salvatierra : Bohr - Gel electrophoresis is a technique for separating DNA fragments depending on various sizes. - It is called "Gel Electrophoresis" because it is based on a movement of charged molecules in an electrical field that occurs in a gel medium. -The process of Gel Electrophoresis needs tools such as a casting tray, gel, comb, electrical supply, and DNA Sample (Different sized DNA Fragments). -The DNA molecules are seen using an agarose gel with ethidium bromide (quickly attaches to DNA molecules). Light brilliant color bands can be seen when the agarose gel containing the DNA is examined under ultraviolet light. - The size or length of a DNA fragment can be determined by comparing the bands obtained to the DNA ladders (standard chart).

[@AnjaliSharma-nv2iv]

Thank you so much🙏 Best explanation till date I got for one of the most important molecular biology techniques -Gel electrophoresis.

[@loubriccant7122]

The best YT channel!

[@nayaabshaikh935]

The explanation is very easy to understand u have done the good thing to teach the students who really want thanks so much video is very clear and easy thnx again ☺️

[@InfinityLearn_NEET]

It's my pleasure. We are glad that you understood the concept. We are happy now that you are all clear about your doubts. You motivate us to do better. Keep watching our videos : )

[@AdeniranTomiwa-wt8re]

Thanks so much for dropping a detailed video, really helpful it's a great one🤗

[@sebastiansanesteban]

San Esteban, Sebastian Angelo L. 8-Chatelet Gel Electrophoresis is a technique to separate DNA of different sizes using the movement of charge molecules using a gel medium. Agarose gel is obtained from seaweeds and is used in gel electrophoresis. Bigger DNA fragments are slower while the smaller ones are faster. Wells are the starting point of DNA fragments that are mixed with color-loading dye for better observation in which when the electric field is made it will make the DNA move towards the positive-charged anode.

[@ayurgreenpalasa785]

Superb the popular coloured dye used for the gel electrophoresis is ethidium bromide 6:43

[@aakashdev3881]

The quality of the video is excellent.... thank u so much............😍😍😍🥰🥰

[@InfinityLearn_NEET]

You're most welcome Aakash. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. Keep watching our videos : )

[@aakashdev3881]

@@InfinityLearn_NEET You r welcome Mam ... I have a request can u make the videos of biotechnology (full chapter) in this manner so that we can understand it in more better way and will get help for the upcoming juniors.......

[@its_anosha_khan]

Just PERFECT ' Perfecttt Lecture '' Thankyou so much ♥️♥️

[@InfinityLearn_NEET]

You're most welcome. We are glad that you understood the concept. We are glad that you understood the concept. You motivate us to do better. Keep watching our videos : )

[@vamsikrishna251]

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[@ashleyrainespedilla5226]

Espedilla, Ashley Rain P. | 8-Bohr - Gel electrophoresis is a technique used to separate DNA based on their sizes. - This separation technique is based on the movement of charged molecules when exposed to an electrical field. - It is called Gel Electrophoresis because the movement occurs in a gel medium. - The DNA is negatively charged because of the presence of phosphate groups. - DNA moves from the negative end to the positive end. - Because the gel has a mesh structure, smaller fragments move faster than larger fragments. - Elution is the process of cutting and extracting DNA fragments from Agarose Gel.

[@colleensalting1138]

Salting, Colleen L. 8-Avogadro -The separation of DNA fragments based on their sizes is called Gel Electrophoresis -Technique is based on the movement of charged molecules occurring in an electrical field; occurs in the Gel Medium -The DNA is negatively charged due to Phosphate groups -The DNA fragments may contain DNA fragments. Because of different sizes those which are smaller move ahead, and those larger in size move slower -The gel has a mesh-like structure, similar to a sieve Gel Electrophoresis: -Since the DNA is negatively charged it will move towards the positively charged electrode -Using a colored loading dye can help identify and track the DNA in the gel since they are invisible DNA separation: -Ethidium Bromide easily binds to the DNA molecules; this helps us see bright-colored bands under UV light -To know the size of the DNA bands a DNA Ladder is used -Elution is the manual extraction of a DNA fragment from the Agarose Gel used -Gel Electrophoresis is considered one of the most important techniques in recombinant DNA technology

[@brendanliamrebote699]

naknam, hi cols

[@laharigam8185]

No words to say this explanation is amazing which is very clearly understandable to students from non biology background also👌👌

[@InfinityLearn_NEET]

Thanks a ton Lalitha for your appreciation. We are glad to hear that it was helpful for you. We hope that you understood the concept. We hope now you are clear your all doubts. Keep watching. 😊😊 😊😊

[@laharigam8185]

@@InfinityLearn_NEET Iam already subscribed ur channel mam plz do more videos on Molecular biology and biophysics techniques

[@aarthipriyapulavarthi902]

@@InfinityLearn_NEET mam but may be small size particles get struck into the pores of gel how can they move faster and why large molecules move slow please do explain.......

[@shan-vw7sr]

Espeleta, Shanea Ainzley C. 8-Chatelet - Gel electrophoresis is the separation of DNA fragments on a piece of gel. - It is based on the movement of charge molecules when exposed to an electrical field and the movement occurs in a gel medium. - DNA is negatively charged due to phosphate groups - One of the most important methods used in recombinant DNA technology is gel electrophoresis. - Agarose gel, which is typically used for DNA analysis, is made from seaweeds.

[@jzpareja6673]

Pareja, Jazper Wayne J. 8-Bohr - Gel electrophoresis is a technique to seperate different fragments of DNA based on their sizes. - Gel electrophoresis happens when the movement of charged molecules are exposed through an electrical field and occurs on a gel medium. - DNA is negatively charged due to the phosphate groups. - Agarose gel is generally used for the DNA and it is obtained from seaweeds. - The gel has a mesh-like structure and the pore size is nearly constant throughout. - Ethidium bromide is used to treat the agarose gel to easily bind to the DNA molecules - Elution is the process of cutting and extracting of the DNA fragment from the agarose gel to be used for downstream processing

[@juliasalonga]

Salonga, Julia Gabrielle P. - Avogadro - Gel electrophoresis is the technique to separate the different DNA fragments based off of their size. - Separation of DNA on a piece of gel. - The separation technique of gel electrophoresis is based off the movement of charged molecules when exposed to electrical fields and these movements occurs in a gel medium. - In gel electrophoresis, fragments that are larger have difficulty in moving whereas smaller particles move ahead. Meaning molecules do not move at the same pace. - The gel has a mesh like structure when looked under a microscope having a constant pore size throughout - Ethidium Bromide is used to treat the Agarose gel in order to observe the DNA because it can easily bind to the DNA molecules. - The cutting out and extraction of DNA fragments form agarose gel is called elution.

[@brendanliamrebote699]

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[@akichu_08]

Fernandez, Akiko B. | 8-Chatelet - Gel electrophoresis is the separation of DNA fragments on a piece of gel. It is based on the movement of charged molecules when expose to the electric field. It was said that Gel electrophoresis is considered a very important technique used in recombinant DNA. - The charge present on the DNA molecule is negatively charged due to phosphate groups. - Agarose gel is the gel used for Gel electrophoresis and it is obtained from seaweeds. - It is said that the smaller fragments move faster than the large fragments, and they move from negative to positive (they can track the DNA moving by using the colored Dye). - To easily bind the DNA molecule, treat the agarose gel with ethidium bromide solution. - DNA ladders will help us know the size of DNA fragments. - Elution is the cutting and extraction of DNA fragments from Agarose gel. - The extraction of DNA can be used for further downstream processing.

[@Anshuman_M]

Thank you so much for this video. It's crystal clear now

[@lychsashyraa8997]

your explanation is incredible, thanks a bunch! it's useful for me

[@idrisgamous4895]

Best explanation ever in my life

[@josepholaoba8476]

Wow wow wow. That's all i can say. Bravo!

[@KyleYnnoCandelaria]

Candelaria, Kyle Ynno D. 8 - Del Mundo - Gel electrophoresis is a technique used to separate DNA fragments according to their size on a piece of gel. - The gel used is Agarose gel that’s extracted from seaweeds - A very important procedure in recombining DNA (Recombinant) - Due to the phosphate groups, the DNA molecule has a negative charge. The charge is applied to help the mobility of the DNA fragments. - To know the size of the DNA fragments, a DNA ladder is used. - Elution is the process of cutting and extracting DNA fragments from the Agarose gel. - The smaller fragments move faster than the big ones and move from negative to positive. - Ethidium bromide solution is applied to the gel to bind the DNA molecules more easily. - To observe the movements of the fragments, a dye is used (must be colored). - The gel has a structure similar to mesh and has pores, the fragments move through the pores

[@corvinntoledo5008]

Toledo, Corvinn D. • Gel Electrophoresis is a laboratory technique used to separate and analyze DNA, RNA, or protein molecules based on their size and charge. • The technique involves placing the sample into a gel, which is then subjected to an electric field, causing the molecules to move through the gel based on their charge. • The gel acts as a molecular sieve, slowing down larger molecules and allowing smaller molecules to move through more quickly. • The separated molecules can then be visualized by staining the gel with a dye, such as ethidium bromide, which binds to the DNA and fluoresces under UV light. There are two main types of gel electrophoresis: agarose gel electrophoresis for separating DNA and RNA molecules, and polyacrylamide gel electrophoresis for separating proteins. • Gel Electrophoresis can be used for a variety of purposes, including DNA fingerprinting, genetic sequencing, and protein analysis. • The technique is widely used in molecular biology research and has many applications in fields such as forensics, medicine, and biotechnology. • Gel electrophoresis is a relatively simple and inexpensive technique, but requires careful attention to detail to achieve accurate and reproducible results

[@MausamGurung-li5li]

Best explanation I have ever seen

[@user-xizg2499s]

Thank you so much.. for simple, nice & clear explaining 🎀

[@MarchaelaArianeAlmazora]

Almazora, Marchaela Ariane C. | 8 - Avogadro - Gel Electrophoresis is the separation of DNA fragment based on their sizes on a piece of gel. - A DNA is negatively charged due to the presence of phosphate groups. - Agarose gel is the gel used for the DNA (obtained from seaweed). - The charged applied across the gel helps in the mobility of DNA fragments. - Larger fragments find it difficult to move which makes them slower than small ones. - The complete set up includes a casting tray, gel, comb, electrical supply, and most importantly the DNA sample. - Coloured loading dye is used to track the movement of the DNA. - To observe the DNA molecules, the agarose gel is treated with Ethidium Bromide. It binds the DNA molecules. - DNA ladders is used to know the size of any DNA fragments. - The process called Elution is the cutting and extraction of DNA fragments from agarose gel. It can also be used for downstream processing.

[@TrixieReinCanlas]

Canlas, Trixie Rein L. 8 - Chatelet - Gel Electrophoresis is the technique to separate the different DNA fragments based on their sizes - It is called gel electrophoresis because it is based on the movements of charged molecules when exposed t.o an electrical field. The movement occurs in gel medium - To observe the DNA molecules, treat the agarose gel with ethidium bromide solution - The major reason for using ethidium bromide is that it easily binds to the DNA molecules and when the agarose gel containing the DNA is observed under the ultraviolet light, bright orange colored bands are clearly seen - The bands obtained are compared with a standard chart known as the DNA ladders

[@ShennaOsoteo]

Shenna Osoteo 8 - Avogadro - Gel Electrophoresis is the separation of DNA Fragments on a Piece of Gel based on their sizes. It is called Gel Electrophoresis because of the movement of charged molecules present in Electrical Field - Agarose Gel are the common type of Gel that is used, and it can be found in Seaweeds. - Ethidium Bromide can be easily binds to the DNA Molecules - Elution is the process of cutting and extraction of DNA Fragments from Agarose Gel, and it is used for Downstream processing

[@RhaneeAnneAnteza]

Anteza, Rhanee Anne E. 8- Bohr Gel Electrophoresis - Separation of DNA fragments on a piece of gel - Technique the different DNA fragments based on their sizes - Movement of charged molecules occurring in an electrical field known as "Gel Medium." - (DNA is negatively charged due to the phosphate group) - a gel used (agarose gel) obtain from the seaweeds - smaller fragments faster while larger fragments would find it difficult to come out - -DNA samples must be placed close to the cathodes and terminals on the negative end for them to travel to the anode on the positive end. Since DNA molecules are colorless, colored loading dye is used to follow the flow of DNA (it also travels a bit faster to reach the terminal end).

[@Mangoeggnogs]

Morgado, Anjanet Mauve, E. || 8-Edison •Gel electrophoresis is a technique used to separate DNA fragments •It is called electrophoresis because it is based on the movement of charged molecules when exposed on an electrical field. •The charged molecules move at different speeds, the larger ones will move slower while the smaller ones will move faster. •The sizes of DNA fragments can be determined by the DNA ladder which shows comparisons of the binds made by Ethidium Bromide. •By the help of the Dna ladder, the desired dna can be easily identified. •Elution is the cutting and extraction of DNA fragment from Agarose gel

[@martheenahernandez-hp4cm]

Hernandez, Martheena D. 8 - Chatelet * Gel Electrophoresis is important to Recombinant DNA Technology. It is the process of separating DNA fragments based on their size. * The term “Gel Electrophoresis” is derived from its process which is the movement of charged molecules over an electrical field happening on a GEL medium. * Agarose gel is the gel used in Gel Electrophoresis and is obtained from seaweeds. * After setting up, the first step is to create a “well-like structure” in the gel using a comb wherein the DNA samples will be placed. The well is placed on the side of the cathode (negative charged side) because DNA are negatively charged (because of the phosphate groups found in the backbone). * Furthermore, to track the DNA, colored loading dye is mixed with the samples. Note that the dye should travel faster when the electric current is turned on. * Once the dye reaches the other end (positive charged side), the electric supply is turned off. * To detect the separated DNA, it is mixed with Ethidium Bromide as this substance binds to the DNA molecules. Also, when exposed to UV light, bright orange colored bands are seen (these are the bands of DNA). * To give an idea of what DNA fragment to extract, a standard chart is used, DNA ladder. * Lastly, Elution is the process of cutting and extracting DNA fragments from the Agarose Gel. This process is used in downstream processing.

[@ishiivv]

Guillermo, Akeisha Chleouna C. | Grade 8-Avogadro Gel Electrophoresis - It is a technique to separate the different DNA fragments based on their sizes - It is called Gel Electrophoresis because this separation technique is based on the movement of charge molecules when exposed to an electrical field and this movement is done in a Gel Medium - A DNA is negatively charged due to Phosphate groups - Not all fragments move the same - The gel used for the DNA is Agarose gel obtained from seaweeds

[@amielenriquez6908]

Enriquez, Amiel M. 8-Del Mundo -Gel Electrophoresis - Technique to separate the different DNA fragments based on their sizes that is also based on the charged molecules exposed into an electrical field. A DNA is Negatively charged because of Phosphate groups -Agarose gel comes from Sea weeds -Ethidium Bromide easily binds to the DNA molecules thus orange DNA Bands are seen in the ultraviolet light. -Elution is the cutting and extraction of DNA fragments from the agarose Gel.

[@gullafshan6040]

The best lectures over electrophoresis 👍🏻👍🏻👍🏻

[@simeoncaluyaiv7443]

Caluya, Simeon IV R.: Edison • Gel Electrophoresis is a process that separates DNA fragments on a piece of gel based on their sizes. • It is called Gel electrophoresis because this technique occurs in a gel medium that is based on the movement of charged molecules when exposed to an electrical field. • DNA is negatively charged due to the phosphate groups in the backbone of the DNA double helix. • Agarose gel is generally used for the DNA that is obtained from the seaweed. • The charge applied across the gel helps in the mobility of fragments. • Smaller DNA fragments will escape the pores faster while the bigger ones will take some time to escape. • The Gel Electrophoresis procedure requires a casting tray, gel, comb, electrical supply, and DNA sample with different-sized fragments. • The wells that load the DNA samples should be formed near the negative terminal. • We can track the movement of the DNA in the gel by using colored loading dye. • The buffer solution is used to provide better conductivity of electricity. • The agarose gel together with the ethidium bromide is used to observe the DNA molecules. Ethidium bromide can easily bind to the DNA molecules and the DNA is seen as bright orange colored bands under ultraviolet light. • The bands obtained are compared with a standard chart or the “DNA ladders” to know the length of the fragments. • Illusion is a process of cutting and extracting DNA fragments from agarose gel.

[@akemi4439]

Cornista, Akeem Marco A. : Edison • Gel Electrophoresis is a a technique to separate the different DNA fragments based on their sizes. • The movements of charged molecules occurs in an electrical field and it all happens in a gel medium, hence it's called Gel Electrophoresis. • The DNA are negatively charged due to phosphate groups. • The gel generally used for the DNA is agarose gel, which is obtained from sea weed. • Ethidium bromide and agarose gel are used to view the DNA molecule (ethidium bromide is used because when the agarose gel containing the DNA is observed under ultraviolet light, bright color bands are clearly seen). • Tools such as a casting tray, gel, comb, electrical supply, and DNA sample are required for the Gel Electrophoresis procedure (Different sized DNA Fragments). • By comparing the bands to the DNA ladders, it is possible to determine the size or length of a DNA fragment.

[@hansthomasinosa5484]

Iñosa, Hans Thomas O. 8-Bohr - The technique of gel electrophoresis is used to separate various DNA fragments according to their sizes. - Gel electrophoresis uses an electric field to move charged molecules through a gel matrix. - Due to the phosphate groups, DNA is negatively charged. - The pore size is nearly constant throughout the gel, which has a mesh-like structure. - DNA is frequently examined using agarose gel, which is made from seaweed. - Agarose gel is treated with ethidium bromide to make it easier for DNA molecules to bind to it. - Elution is the process of cutting and extracting DNA fragments from Agarose gel.

[@hanifazam1824]

a lot of question are clear that have in my mind i am really thankful......................

[@rimshaasif689]

This was amazing! Thank you

[@InfinityLearn_NEET]

It's my pleasure. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. Keep watching our videos : )

[@maricelcristobal2696]

Cristobal, Adam Matthew Gel electrophoresis separation of DNA Fragments to a piece of gel -technique to separate the diff DNA fragments Based on their size -it is based of movement of charged molecules occurring in electrical field -is generalized on agarise gel

[@kamakshiwahi1111]

It is simply amazing.... Hats off to your hardwork👏👏💯

[@maryjoycezalamea1722]

Zalamea, Mary Joyce H. 8 - Edison Gel Electrophoresis - Gel electrophoresis is a method used for separating DNA fragments based on their sizes using Agarose gel, a gel that can be obtained from seaweeds. - A DNA molecule is negatively charged due to the phosphate groups found in its double-helix structure. - A colored loading dye is added to the mixture to easily track the DNA due to it being colorless. - DNA samples are loaded near the negative electrode side of the gel as they move towards the positive electrode using the electric current. - Agarose gel is treated with Ethidium Bromide solution, as Ethidium Bromide can easily bind to the DNA molecules. - Elution is the process of cutting and extracting DNA fragments from Agarose Gel.

[@brendanliamrebote699]

ge

[@Al-jj7uz]

Ditona, Michaela Gabrielle C. 8- Chatelet Gel electrophoresis is a technique to separate the different DNA fragments by their size. We can extract Dna with gel electrophoresis using : A container, a comb, an electrical supply, and DNA samples It's considered as one of the very important techniques used in recombinant DNA technology It is negatively charged Gel that is commonly used is agarose gel, which is obtained from seaweeds

[@akshayasasi7091]

Crystal clear....explanation is on point

[@mekaellavelasco8909]

Velasco, Mekaella 8 - Chatelet A technique called gel electrophoresis divides DNA fragments in a gel based on their sizes. Due to the fact that this method is performed in a gel medium and is based on the movement of charged molecules when exposed to an electrical field, it is known as gel electrophoresis. Gel, a comb, an electrical source, and a DNA sample with various-sized fragments are all required for the Gel Electrophoresis procedure. Near the negative terminal, the wells that load the DNA samples should be created. Using loading dye, we can observe how the DNA moves within the gel. To improve electrical conductivity, the buffer solution is used. Ethidium bromide and the agarose gel are used to observe the DNA molecules. To know the length of the fragments, we compare the bands obtained in standard chart or the "DNA laders".

[@alexabandada]

Bandada, Alexa Marie H. 8-Chatelet - Recombinant DNA technology uses the technique of gel electrophoresis, which involves sorting DNA fragments on a piece of gel according to their sizes. - Gel electrophoresis is the name given to this method of separation because it is based on the movement of charged molecules in an electrical field, where the movement takes place on a gel medium. - When a DNA sample that contains fragments of different sizes is loaded onto a gel, the charge that is applied throughout the gel helps in the mobility of the fragments. However, not all fragments move at the same rate; smaller fragments move ahead while larger fragments are finding it hard to move. - A gel's wells at one end are loaded with DNA samples, which are then pulled through the gel by an electric current. -The DNA fragments will move in the direction of the positive electrode because they are negatively charged (because of the presence of phosphate groups).

[@dananthoneybasabe8474]

Basabe, Dan Anthoney B. Bohr -‌DNA is negatively charged due to the presence of Phosphate groups. -‌Since that DNA is negatively charged, it will move to the positive electrode side. -‌Gel electrophoresis is a technique used to separate different DNA fragments based on their sizes. -‌It is based on the movement of charged molecules when exposed to an electrical field. -‌This occurs in a gel medium known as Agarose gel. This specific gel medium has a mesh-like structure and pore size constant throughout. -The larger DNA fragments in this medium lag behind while the smaller DNA fragments move faster. -‌Ethidium Bromide is used to easily bind to the DNA molecules -‌The process of manually cutting out and extracting DNA molecules from Agarose Gel is Elution

[@shyny8463]

ty so much for such an amazing explanation

[@InfinityLearn_NEET]

Most welcome, Shyny! Keep watching! 🙂🙂

[@aazmaturky70]

Ur way of explanation is really amazing

[@harlenekimoliveros110]

Harlene Kim C. Oliveros 8-Bohr •Gel Electrophoresis is the method of separating DNA fragments on a piece of gel. It is separated based on their sizes. •The separation is based on the movement of charged molecules when exposed to an electrical field and it occurs in a Gel Medium. •The gel that is used in DNA is the Agarose Gel •Not all segments move at the same phase: Small size-move ahead, Larger size-find it difficult to move. •We can't see a DNA molecules when it is separated.We can observe the separated segments, we trade Agarose Gel with Ethidium Bromide Solution. •Why Ethidium Bromide Solution? ait easily binds to the DNA molecules. •We can see DNA segments with Agarose Gel by ultraviolet light. •Elution is when we cut or extract DNA fragments from Agarose Gel.

[@rainierbernales1938]

Rainier D. Bernales | 8 - Chatelet - Gel electrophoresis is the separation of DNA fragments on a piece of gel. It's based on the movement of charged molecules when exposed to an electric field. - It's an important technique in Recombinant DNA. - Due to phosphate groups., the charge of the DNA molecules are negative. - Agarose gel is a type of gel used for Gel electrophoresis and is obtained from seaweeds. - Agarose Gel with Ethimium Bromide helps in easily binding to the DNA molecules. - Smaller fragments move faster than the large fragments in which they move from negative to positive. - DNA Ladders are used in helping us know the size of the DNA fragments. - Elution is the cutting and extraction of DNA fragments from Agarose gel. - The extraction of DNA can be used for further downstream processing.

[@higgsboson67]

Thank you so much...Cleared all doubts 👍👍

[@marquishasantos566]

Santos, Marquisha R. || Edison - The method used to separate the various DNA fragments according to their sizes is called gel electrophoresis. - Because it is based on the motions of charged molecules when subjected to an electrical field, it is known as gel electrophoresis. The gel medium also known as agarose gel allows for the mobility. - The DNA molecules are easily connected by ethidium bromide. - Elution is a technique for splitting and extracting small amounts of DNA from Agarose Gel.

[@madyj31]

Thank you so much!!🥰

[@briqnnq11]

Dimatulac, Frances Brianna C. - Gel electrophoresis is a technique to separate the different DNA molecules based on their sizes. DNA is negatively charged and this is due to the presence of phosphate groups. - Ethidium Bromide easily binds to the DNA molecules. - Elution is the cutting and extraction of DNA fragments from agarose gel. - The movement of charged molecules is exposed in electrical field and it occurs in gel medium, that's why it's called Gel electrophoresis. - Gel electrophoresis is the name given to this method of separation because it is based on the movement of charged molecules in an electrical field, where the movement takes place on a gel medium.

[@alexajoycenunez4677]

Nuñez, Alexa Joyce M. - Gel electrophoresis is the process used to separate DNA fragments according to their sizes. - It is a method of separation based on the movement of charged molecules in a gel medium when exposed to an electrical field; this is why it is known as gel electrophoresis. - The presence of phosphate groups causes DNA to be negatively charged. - As the DNA in the gel is invisible, using a colored loading dye can help detect and track them. - Elution is the process of manually cutting apart and extracting DNA molecules from a gel medium. - One of the key methods used in recombinant DNA technology is gel electrophoresis.

[@justindaria9256]

Justin Brielle M. Daria: Chatelet - Gel Electrophoresis is a technique to separate different DNA fragments based on their sizes. It uses the electricity to move the negatively charged DNA fragments through the gel which has a mesh like structure that acts like a sieve. To observe this, the Ethidium Bromide is added to the Agarose Gel and then observed under Ultra Violet light. After the process is done, the DNA fragments are extracted through a process called Elution.

[@zenithpotter8797]

Very thoroughly explained. Thanks!

[@KatrinaGubat]

Katrina ysabel G. Gubat (8-BOHR) Gel Electrophoresis - gel electrophoresis is a technique to separate the different DNA molecules based on their sizes - this separation technique is based on the movement of charged molecules when exposed to an electrical field and this movement occurs in a gel medium hence it's known as gel electrophoresis - the gel which is generally used for the DNA is agarose gel and it's obtained from the seaweeds - the gel that is prepared for the experiment here at the microscopic level we find that the gel appears to be a mesh-like structure and the pore size is nearly constant throughout - it's considered as one of the very important techniques used in recombinant DNA technology

[@sbrnrd]

Bernardo, Sophia Marcela O. : Del Mundo ➤Gel Electrophoresis is a technique to separate the various DNA fragments according on their sizes. ➤The separation technique is based on the movement of molecules when exposed to an electrical field. ➤The DNA is negatively charged due to the presence of phosphate groups and phosphate is negatively charged and is found in the backbone of the DNA double helix. ➤Not all fragments move at the same pace; small sized fragments will escape the pores faster while the larger fragments will find it difficult to come out. ➤The Gel Electrophoresis Requirements are casting tray, Agarose Gel, Comb, Electrical Supply, and the DNA sample with different sized DNA fragments. ➤We treat the Agarose Gel with Ethidium Bromide to observe the separated DNA segments because Ethidium Bromide easily binds to the DNA molecules. ➤The bands obtained are compared with a standard chart known as the "DNA Ladder." ➤Elution is the process of cutting and extraction of DNA fragment from Agarose Gel. ➤Gel Electrophoresis is considered as one of the very important techniques used in recombinant DNA Technology.

[@LeanAspiras]

Aspiras, Mary Leana D. | 8-Avogadro - Gel Electrophoresis is the separation of DNA fragments based on their sizes with the use of Agarose gel which can be obtained from seaweeds. - It is known as "Gel Electrophoresis" since it is based on the movement of charged molecules in a gel medium when exposed to an electrical field. - DNA is negatively charged due to the presence of phosphate groups in the backbone of its double helix. - Due to the various size of fragments, smaller in size moves faster, and the larger ones find it difficult to move. - The gel used is a mesh-like structure and the pore size is almost constant. - Since DNA is negatively charged, the DNA fragments are loaded near the negatively charged electrode so they will move to the positively charged anode/electrode. - Colored loading dye is used to track the DNA since it is colorless. - The Agarose gel is treated with Ethidium Bromide solution to clearly see the bands of the DNA under UV light since Ethidium Bromide easily binds to the DNA molecules. - Any DNA band will be compared to a DNA ladder to know their sizes. - Once the desired DNA is identified, it will undergo a process called Elution, which is the cutting and extraction of a DNA fragment from the Agarose gel. - Gel Electrophoresis is considered one of the very important techniques in recombinant DNA technology.

[@CJReyes-su6eo]

Reyes, Ralph CJ D. 8-Chatelet GEL ELECTROPHORESIS - A method of separation of DNA fragments by size with the use of gel. - The separation technique is based on the movement of charged molecules when exposed to an electrical field and it occurs in Gel Medium. - is a method that separates DNA or RNA based on it's sizes - One of the most important methods used in recombinant DNA technology is gel electrophoresis. - Electrophoresis enables you to distinguish DNA fragments of different lengths.

[@hafsarasheed9298]

Superb.Thank you for such an amazing lecture

[@aristotleeducation7282]

Thank you so much. My beloved youtube chanel

[@InfinityLearn_NEET]

You're most welcome. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. Keep watching our videos. 😊😊

[@notsohan95]

amazing , its as clear as water and easy to understand

[@Dehrandomnub]

Steven P. Oliveros (8 Delmundo) Gel Electrophoresis -Gel Electrophoresis is a Technique to seperate the different DNA fragments based on their sizes. -Based on the movement of charged molecules when exposed to an electrical field this movement occurs in a gel medium -The gel used is an Agarose Gel -The seperation is based on the size of the fragments because the smaller fragments moves quicker while the larger ones move less. -To be able to know the scale of how big the DNA fragments are, it is compared to the standard chart known as the DNA ladder. -The Cutting and extracting a DNA fragment from the Agarose Gel is called Elution.

[@knowledgeandinformations3565]

Ever The best video on this topic 🤗

[@athenanoelle]

Cruz, Athena Noelle B. | 8- Del Mundo Gel Electrophoresis: - A method of separation of DNA fragments by size with the use of gel. - This separation technique is based on the movement of charged molecules when exposed to an electrical field that occurs in a gel medium. - The DNA is negatively charged due to the presence of Phosphate groups. which are negatively charged and present in the backbone of the DNA double helix. - The gel used in DNA is Agarose which is obtained from seaweeds. - Not all fragments travel at the same pace; little sized fragments will escape the pores faster while the larger particles will find it difficult to come out. - The necessary components for Gel Electrophoresis are Casting tray, Agarose Gel, Comb, Electrical Supply, and the DNA sample with various-sized DNA fragments. - Ethidium Bromide is used to treat the Agarose Gel in order to observe the split DNA segments since it attaches to DNA molecules effortlessly. - The resulting bands are matched to a commonly used diagram called the "DNA Ladder." - Elution is the method for cutting and removing DNA fragments from Agarose Gel.

[@jerscielouviebonita5725]

Bonita, Jerscie Louvie M. Edison - Gel Electrophoresis is the technique to separate the different DNA fragments based on their sizes. The separation technique is based on the movement of charged molecules when exposed to an electrical field and it occurs in Gel Medium. - The seperation is successful based on the size of the DNA fragments. The larger molecules move slowly. The smaller molecules moves faster. - To identify the size of the fragments - it is compared with a standard chart called "DNA Ladder". - Once we know the length of the fragments, we can now identify the desired DNA. Desired DNA can be cut and extract from the Agarose Gel is called "Elution" and can be used for downstream processing.

[@aaroncamporedondo5223]

Camporedondo, Aaron Michael, S.: CHATELET - Gel Electrophoresis is the technique to separate the different DNA fragments based on their sizes. The separation technique is based on the movement of charged molecules when exposed to an electrical field and it occurs in Gel Medium. - Gel electrophoresis is said to be one of the important techniques in recombinant DNA tech. - The major reason for using ethidium bromide is that it easily binds to the DNA molecules and when the agarose gel containing the DNA is observed under the ultraviolet light, bright orange colored bands are clearly seen - One of the most important methods used in recombinant DNA technology is gel electrophoresis.

[@lancedimaano6755]

Dimaano, Elwyn Lance S. 8-Del Mundo - Gel Electrophoresis is the process of separating DNA fragments according to their sizes. - Based on the movement of charged molecules when exposed to electrical field. It occurs in the Gel Medium therefore it's known as its name. - DNA is negatively charged due to the appearance of Phosphate groups. - The gel used for DNA is Agarose which is obtained from sea weeds - When a DNA sample containing different sizes is loaded onto a gel then the charge applied across the gel helps in the mobility of these fragments - Not all fragments move at the same pace. Those that are smaller move ahead and those that are larger find it hard to move. - Gel Electrophoresis is considered one of the important techniques used in recombinant DNA technology Materials used: - Casting Tray - Gel - Comb - Electrical Supply - DNA sample with different sized DNA fragments - To see the DNA molecules, treat the Agarose Gel with Ethidium Bromide - Ethidium Bromide easily binds to the DNA molecules - To see the size of the DNA fragments, the bands obtained are compared to the standard chart known as the DNA ladder - Elution - The cutting and extraction of DNA fragment from Agarose Gel - The extraction of DNA can be used in such a way that it can be used for further Downstream processing

[@MartinaBiancaSantamaria]

Martina Bianca Santamaria | Grade 8 - Del Mundo Gel Electrophoresis: - A method of separation or separation technique of DNA fragments by size with the use the of gel - In a gel medium, movements of charge molecules occur in an electric field. - Gel used in DNA is Agarose gel from seaweeds Principle of Separation of DNA Fragments: - Size of the parameter is used for the separation. The charge of a molecule helps with the mobility of the fragments but not all fragments move at the same phase. Smaller fragments are ahead of bigger fragments because of the constant size of the pores where the fragments will pass through Note: - In a DNA molecule, negative charge is present due to the phosphate groups. These are present in the backbone of DNA double helix impacts the negative charge. - The DNA molecules will move from negative cathode to the positive charge anode by the use of electric field with the help of buffer solution to conduct electricity better Process: - DNA dye is added to the DNA for us to be able to track the DNA because it is colorless, and it’s selective in such a way that it travels a bit faster than the DNA segment. Once the dye reaches the anode, then it’s the cue to turn off the power supply. After the Process: - We can't really see the separated DNA segment, unless the agarose gel would be treated with Ethidium Bromide and observed through ultraviolet light. Under the UV light we would see DNA Bands, and the separation by DNA fragments is successful. Analyzation: - To know the size of the DNA fragment, there’s a chart called “DNA Ladder." Once compared with chart, we can now easily identify the desired DNA fragment to be cut out and extracted. The cutting and Extraction of DNA fragments from Agarose gel is known as “Elution.”

[@akashikvai]

Your the best teacher maam

[@ElyssaVolante]

Volante, Elyssa Reian S. EDISON - Gel Electrophoresis - is the process of SEPERATING DNA fragments on a piece of gel (Agarose gel) - Wells - are formed near the NEGATIVE side. Since DNA is negatively charged, the fragments will move from the negative to the positive side. - Colored loading dye - is used to TRACK the movement of the fragments. - Buffer solution - is used for better CONDUCTIVITY. - Ethidium Bromine solution - is applied to Agarose gel to OBSERVE the seperate fragments as it easily binds to DNA molecules. - Bright orange bands = Bands of DNA - DNA ladder - DNA bands are compared to the DNA ladder to know their SIZE. - Elution - is the process of EXTRACTING the desired DNA fragment

[@prodyumna2826]

Beautifully Explained Ma'am.. Thank you

[@InfinityLearn_NEET]

You're most welcome and Thank you so much for your appreciation. We are glad that you understood the concept. Do support us by subscribing to our channel. 👍👍

[@nidanazirarife6210]

Perfect

[@biologywithannie]

Absolutely love the graphics...

[@JanMartinVillarico]

Villarico, Jan Martin R. (8 - Edison) - Gel electrophoresis is a method that separates DNA or RNA based on it's sizes. - DNA is negatively charged because of Phosphate groups located in it's backbone. While Ethidium Bromide binds DNA molecules. - DNA samples move from the negative anode to the positive anode in the gel medium.

[@tiachen7701]

Tia A. Chen | 8 - Avogadro - Gel Electrophoresis is a technique to separate the different DNA molecules based on their sizes. - This separation technique is based on the movement of charged molecules when exposed to an electrical field, and this movement occurs in a gel medium, hence known as Gel Electrophoresis. - DNA is negatively charged, and the gel used for DNA is an agarose gel. - The charge applied across the gel helps in the mobility of the fragments. However, not all fragments move at the same pace. To further understand the principle of separation of DNA Fragments based on their sizes, those who are smaller move ahead, and those larger find it difficult to move. - DNA Fragments being negatively charged will travel from the point of negative charge to the point of positive charge. Thus, the wells will be loaded in the cathode or negative terminal. - The colored loading dye is also used to track the movement of the DNA. - To observe the DNA molecules, agarose gel is treated with Ethidium Bromide Solution, which easily binds to the DNA molecules and produces bright orange-colored bands when observed under ultraviolet light. - The DNA ladder is the standard chart that is used to estimate the size of the DNA bands in experimental samples. - Lastly, Elution is the process of cutting and extracting DNA fragments from the Agarose Gel.

[@hannahjasleenhomeres3219]

Homeres, Hannah Jasleen A. (8- Edison) - Gel Electrophoresis is the separation of the DNA Fragments on a piece of gel. - It is a technique to separate the different DNA molecules based on their sizes since it is one of the most important process in recombinant DNA. - This separation technique is based on the charged molecules when exposed to electrical field and it occurs in a gel medium. - The charge of a DNA molecule is negatively charged due to the presence of phosphate groups. - The phosphate which is in negative charge and present in the backbone of the DNA double helix impart a negative charge to the molecule. - Agarose Gel is a gel generally used for DNA and It is obtained from sea weeds. - The pores that have small in size can be easily escape whereas the bigger size pores will be retained back. - The first requirement in order to work with Gel Electrophoresis is a complete set up including the casting tray, gel, comb, electrical supply and DNA sample. - To observe the separating segments of the DNA molecules, we treat the Agarose Gel with Ethidium Bromide which easily binds to the DNA molecules. - The larger molecules are the ones that moves slowly while the smaller molecules are the ones that moves fastly. - The DNA Ladder bands obtained are compared with a standard chart. - Elution is the process of cutting and extracting of DNA fragment from Agarose Gel. It is used for Downstream processing.

[@Cunnuu]

Thankyou very much ❤😊