Preparing an Agarose Gel For Electrophoresis - Edvotek Video Tutorial

[@kewltodamax]

This vid shud be the most watched at a time like this. PCR is bloody important as of this moment to detect sars-cov-2 presence

[@kwekker]

Thank u very much this spared me a lot of time looking up how to do it

[@mrvyper8553]

Thanks for making the full procedure ...👍

[@LEGENDGAMING-ns6gw]

Thanks for full preperation

[@lucaslopez2091]

excelente video, todo claro, ¡gracias!

[@LEGENDGAMING-ns6gw]

Thank you

[@CNkids73]

Excellent 👍👍👍

[@merveyavuz7310]

should we add a fluorescent dye in order to observe running of DNA/RNA such as EtBr?Cant we observe with such preperation you did?

[@EdvotekInc]

+Merve YAVUZ Agarose gels can be stained in many different ways. We did not add EtBr in this protocol because we wanted to keep it very general. Here is more information on the DNA stains Edvotek provides www.edvotek.com/site/pdf/DNA_Stain_Guide.pdf

[@sislam5154]

very helpful

[@EdvotekInc]

Thanks, glad you liked it!

[@choomiealhajaff2925]

Greetings.. im just wondering, most of the agarose gel prep video didnt add any EtBr in their solution including this one. however, i've been using Etbr for the agarose gel prep in my research. Is it okay if i didnt add the EtBr? Will it make any difference?

[@EdvotekInc]

+choomie Fitri Many of our customers are not allowed to use Ethidium Bromide in their classrooms, so we do not feature its addition in this video. If you do not add Ethidium Bromide to your gel, you will need to stain the gel post electrophoresis. This playlist (kzbin.info/aero/PLdpmHMO6NC2ziJIs3i3uZrWe6zHEV5vpL) features our post-gel stains. For research purposes, you may want to continue adding liquid Ethidium Bromide to your gels. You would add the Ethidium Bromide liquid to the gel after the molten agarose has cooled, but before pouring the gel. Be sure to swirl the agarose to mix with the stain before pouring the gel.

[@warisachanthet8518]

Cheers!

[@marishashanthikumar6173]

We could use sybersafe instead of EtBr yeah

[@communitycollegeassociatio1310]

It's possible to use an alternative that is not a mutagen. We use gel red stain and it can be added after the agar is boiled. If you are only prepping a gel to show how different dyes run, then you wouldn't need the EtBr.

[@paouw6877]

what can be used as buffer solution except for tbe and tae?

[@EdvotekInc]

It depends on what you are trying to accomplish. Some home scientists will use lemonade mix because of the citric acid, which acts as a buffer. But, for research purposes, you should use a buffer made from commercial-grade chemicals.

[@wearit23]

Excellent! 🌸

[@gerjaison]

What's the power rating for the microwave?

[@EdvotekInc]

I'm not sure of the exact power rating for that microwave. The most important thing is to heat in shorter bursts to prevent the solution from boiling over.

[@axellefrancois387]

merci le s

[@bosycaramela]

Please can you turn off the music in other videos

[@mohamedacro521]

Thanks for these video

[@mannequia8294]

"Dissolve the agarose powder by boiling the solution." Pulls out a f*cking microwave

[@EdvotekInc]

If you don't want to use a microwave, there are many other ways to boil the solution, like a hot plate. What resources do you have in your lab?

[@lucaslopez2091]

seriously, you have to act like that

[@mriduchaudhary9111]

Thanks sir

[@isabelfernandezmenacho1415]

Super chuli