Agarose Gel Electrophoresis

Рет қаралды 268,102

Күн бұрын

Пікірлер: 41

@addgene 2 жыл бұрын

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

@ruchithruchi5009 Жыл бұрын

!!! only video which tells how much DNA concentretion should load in well !! thankyou for the clear explaination keep it up..

@marksantacruz8892 5 жыл бұрын

Gonna do this in my lab tomorrow, exciting!

@ryanbaker2040 4 жыл бұрын

Same

@ishika11________ Жыл бұрын

fav channel for videos from now on

@besmile2257 3 жыл бұрын

Wow! Addgene videos have been very very helpful for me. THANKS A LOT!!!

@addgene 3 жыл бұрын

You're very welcome. Thanks for watching!

@mpumigama2587 3 жыл бұрын

Thank you so much for this clear description. Came to confirm if I use the same buffer I used to make gel in the box.

@bhavanapatel9681 3 жыл бұрын

It's amazing, I got it really easy , it helped me a lot , thank you very much

@Misha2010Tube 4 ай бұрын

4:15 Wow, the gel is literally running from left to right! What kind of force pushes the whole gel slab to the positive electrode overcoming the friction? Why don't I see the running gel slabs in my experiments?

@jnanendramajumder8394 2 жыл бұрын

Gel electrophoresis process demonstration very nice .

@Matthias27182 4 жыл бұрын

The knowledge in the video is very helpful, but the music in the background was a bit too loud; it sounded like an annoying phone call that wasn't being answered

@addgene 4 жыл бұрын

Thanks for the feedback! We've been working hard on getting the mix right on our recent videos, so we'll keep this in mind.

@lovemyself-wl4sz 11 ай бұрын

Ohhh thanks alot...needed in med school❤

@kaushanichanda2583 3 жыл бұрын

One of the best explaination

@ronaldbarrett1506 3 жыл бұрын

Very good video. Thank you. Learning lots here.

@ishika11________ Жыл бұрын

make videos on microarray, rnaseq, dna sequencing , cloning, etc

@genicadelara5243 4 жыл бұрын

Very clear explanation. Thank you

@jnanendramajumder8394 2 жыл бұрын

Nice vdo for gel electrophoresis ,thank you .

@ReemMohamed-p5q Жыл бұрын

does gel electrophoresis separate DNA fragments according to density or size?

@PravinPatil-m9b Жыл бұрын

Very nice 👍👍

@storieswithmusab Жыл бұрын

Thank you very much 🙂

@lethuytonhu7385 3 жыл бұрын

Please help me, how to prepare DNA ladder before loading and how many volt to let the ladder separate beautifully. Thanks

@addgene 3 жыл бұрын

Our written protocol has some more details to help you out: www.addgene.org/protocols/gel-electrophoresis/ We also recommend following the manufacturer’s instructions for the DNA ladder you choose for your research. Good luck!

@641-murtazapatangwala4 3 жыл бұрын

You can keep the ratio i.e. 5volts for 1cm distance between the two corners of the gel .i.e. -l (no. of centemeters x 5) = no. of volts l+

@gyrothompson 11 ай бұрын

Is it safe to touch the gel?

@muntazirhashim8809 Жыл бұрын

Thank you ❤

@kaushanichanda2583 3 жыл бұрын

What are the buffer used to prepare the DNA samples?

@addgene 3 жыл бұрын

Hello, thanks for the question! Most commonly, people use TAE (which is what we're using in this video). You can find a recipe for it here: media.addgene.org/cms/files/TAERecipe.pdf TBE is another common buffer sometimes used, which you can find a recipe for here: cshprotocols.cshlp.org/content/2006/1/pdb.rec8458.full We also have a very short video explaining some of the differences between TAE and TBE: kzbin.info/www/bejne/m4LUmYKJpZ1jh8k

@RonitDebnath-sv8nr 6 ай бұрын

Thank you

@amirhoseinabdorahimzadeh1648 3 жыл бұрын

why does the gel itself move right at 4:10?

@addgene 3 жыл бұрын

Thanks for your question! Sometimes the current created by the gel box is enough to move the whole gel itself, but that shouldn’t impact your DNA’s movement through the gel.