Agarose Gel Electrophoresis

[@addgene]

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to [e-mail] as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

[@ruchithruchi5009]

!!! only video which tells how much DNA concentretion should load in well !! thankyou for the clear explaination keep it up..

[@marksantacruz8892]

Gonna do this in my lab tomorrow, exciting!

[@ryanbaker2040]

Same

[@mpumigama2587]

Thank you so much for this clear description. Came to confirm if I use the same buffer I used to make gel in the box.

[@Matthias27182]

The knowledge in the video is very helpful, but the music in the background was a bit too loud; it sounded like an annoying phone call that wasn't being answered

[@addgene]

Thanks for the feedback! We've been working hard on getting the mix right on our recent videos, so we'll keep this in mind.

[@besmile2257]

Wow! Addgene videos have been very very helpful for me. THANKS A LOT!!!

[@addgene]

You're very welcome. Thanks for watching!

[@ishika11________]

fav channel for videos from now on

[@bhavanapatel9681]

It's amazing, I got it really easy , it helped me a lot , thank you very much

[@jnanendramajumder8394]

Gel electrophoresis process demonstration very nice .

[@Misha2010Tube]

4:15 Wow, the gel is literally running from left to right! What kind of force pushes the whole gel slab to the positive electrode overcoming the friction? Why don't I see the running gel slabs in my experiments?

[@lovemyself-wl4sz]

Ohhh thanks alot...needed in med school❤

[@kaushanichanda2583]

One of the best explaination

[@ishika11________]

make videos on microarray, rnaseq, dna sequencing , cloning, etc

[@ronaldbarrett1506]

Very good video. Thank you. Learning lots here.

[@genicadelara5243]

Very clear explanation. Thank you

[@jnanendramajumder8394]

Nice vdo for gel electrophoresis ,thank you .

[@ReemMohamed-p5q]

does gel electrophoresis separate DNA fragments according to density or size?

[@storieswithmusab]

Thank you very much 🙂

[@PravinPatil-m9b]

Very nice 👍👍

[@gyrothompson]

Is it safe to touch the gel?

[@RonitDebnath-sv8nr]

Thank you

[@EducationFailed]

The music becomes so loud I can not hear what is being said.

[@lethuytonhu7385]

Please help me, how to prepare DNA ladder before loading and how many volt to let the ladder separate beautifully. Thanks

[@addgene]

Our written protocol has some more details to help you out: www.addgene.org/protocols/gel-electrophoresis/ We also recommend following the manufacturer’s instructions for the DNA ladder you choose for your research. Good luck!

[@641-murtazapatangwala4]

You can keep the ratio i.e. 5volts for 1cm distance between the two corners of the gel .i.e. -l (no. of centemeters x 5) = no. of volts l+

[@حنينفارسعبدمتعب]

It's very well but is so fast that you said 🥺💔😍

[@muntazirhashim8809]

Thank you ❤

[@kaushanichanda2583]

What are the buffer used to prepare the DNA samples?

[@addgene]

Hello, thanks for the question! Most commonly, people use TAE (which is what we're using in this video). You can find a recipe for it here: media.addgene.org/cms/files/TAERecipe.pdf TBE is another common buffer sometimes used, which you can find a recipe for here: cshprotocols.cshlp.org/content/2006/1/pdb.rec8458.full We also have a very short video explaining some of the differences between TAE and TBE: kzbin.info/www/bejne/m4LUmYKJpZ1jh8k

[@cristianedasilvaalves772]

Obrigada

[@ronikakashyap387]

Nice

[@sarahafrin3493]

Very well but you are going very fast.

[@amirhoseinabdorahimzadeh1648]

why does the gel itself move right at 4:10?

[@addgene]

Thanks for your question! Sometimes the current created by the gel box is enough to move the whole gel itself, but that shouldn’t impact your DNA’s movement through the gel.

[@tianayoung2338]

Who is here cuz of K12?

[@MaideCanyakar]

👍🏻

[@mavicone5845]

She didn’t change her gloves when she handled the gel and then touched the microwave .. yikes contamination factor

[@AidanReed-w1m]

nerds