Enumeration of Escherichia coli (Total E. coli Count)_A Complete Procedure (ISO 9308-1 & ISO 16649)

[@lithupaul5316]

This video's really helpful for we microbiologist .. thank you

[@MicroChemsExperiments]

Lithu Paul. You are most welcome. It's our pleasure. Thanks for being with us.

[@saniketbhosle3390]

Can I use this method for milk products like Ice-creams?

[@brianmarv]

Is this a Most Probable Number (MPN) method or Membrane Filtration Method (MFM)? Can it work for turbid water?

[@hellynlight]

Pls what if I want to further get different strains of E. coli, how do I go about that

[@JohnClintGodinez]

how good morning, can you make a video for conventional test for product like Lotion, Sunscreen, or rexona, to dtermine if the product is good to use or not..

[@MicroChemsExperiments]

Noted

[@balajiraam6131]

Hii mam i have a doubt mam why we mix peptone salt to salt mam??? What purrpose we do it mam please tell?

[@MicroChemsExperiments]

Peptone salt solution is ideal diluent for microbiological analysis as mention by ISO guidelines.

[@JohnClintGodinez]

hope you can give some sample video, on how to perform conventional test on essentials product like, lotion, sunscreen etc. to know if the is ok to use or not.. or their are bacterial growth on the product itself, thanks in advance ,& Godnless.. 😘

[@MicroChemsExperiments]

Noted

[@JohnClintGodinez]

what are the common bacteria on essentials product mr/miss microchems??

[@nurulashikin4633]

Hi..thank you for this video..may i know if i can substitute the peptone and NaCl with bacteriological peptone?...and can we confirm the presence of e.coli by dropping kovacs reagent on violet colonies? can i know where can i get the documented ISO protocol for this video? or is this validated in house method?

[@MicroChemsExperiments]

You can use bacteriological peptone. Don't drop kovac's reagent directly. Do it as done in this video. You can purchase the protocol from ISO website

[@nurulsuhana9974]

if i use samples from feces of goats, can I use the method same as the soil for the sample preparation part?

[@MicroChemsExperiments]

Yes. You can

[@zarqaarshad411]

I use macconkey agar in place of sorbitol macconkey agar

[@JohnClintGodinez]

conventional test for lotion, or sunscreen Product can you help me... thanks in advance..

[@MicroChemsExperiments]

Noted

[@almirahayunan6868]

Our laboratory doesn't have stomacher machine, what should we use as a substitution? Is it okay to shake it by hand? Second question is that, how much water sample should we dilute?

[@MicroChemsExperiments]

You can shake with hand properly to make homogeneous mixture. Dilution 25ml of sample into 225ml diluent to make 10 times Dilution

[@almirahayunan6868]

Thanks for responding, i have one question, is there any agar substitute for chromocult agar?

[@MicroChemsExperiments]

@@almirahayunan6868 no

[@almirahayunan6868]

Okay, thank you so much. God Bless.

[@amiyavb8275]

If we make dilution how will count the dilution factors ( DF)❔ and one more question is if we done 10 dilution how will the -1, -2 dilutions in trial 1 and 2 cca plates ?

[@MicroChemsExperiments]

You need to learn about serial dilutions and it's calculation

[@lithupaul5316]

Could you please tell me which microbiological analysis method you are following...

[@MicroChemsExperiments]

Lithu Paul, Reference method is mentioned at the starting of the video. Method number is also mentioned in the Title and video description. Please check it out. Thanks.

[@zarqaarshad411]

Can I use mfc agr in place of chromocult agr if I use then which color gave E.coli colony

[@MicroChemsExperiments]

Yes you can use. Blue colonies on m-FC agar

[@zarqaarshad411]

But fecal coliform and E.coli both blue color on mfc agar then how to differentiate which is E.coli and fecal coliform

[@SnoooooowMan]

Can we substitute cca with tryptone bile agar?

[@MicroChemsExperiments]

You can

[@Danurdn903]

Can we substitute peptone and NaCl with Buffered Peptone Water?

[@MicroChemsExperiments]

Yes. You can

[@Danurdn903]

@@MicroChemsExperiments Thank you for the answer.

[@MicroChemsExperiments]

@@Danurdn903 you are welcome

[@mohdsaleem3349]

Can you do videos of microbiological analysis as per USFDA BAM

[@MicroChemsExperiments]

kzbin.info/www/bejne/f523n397nM6pqtU

[@Unknown-fw8yy]

Hi, how can we use this method in water test for e coli? What should we do?

[@Unknown-fw8yy]

Is it right to mix the cooled dilute peptone salt solution in the water sample? If not, what should the right thing to do?

[@MicroChemsExperiments]

This method is also valid for water sample.

[@MicroChemsExperiments]

You can dilute water sample using peptone salt solution

[@Unknown-fw8yy]

Our laboratory doesn't have stomacher machine, what should we use as a substitution? Is it okay to shake it by hand?

[@Unknown-fw8yy]

Second question is that, how much water sample should we dilute?

[@sunitakarki9694]

Can we use this method to detect E. Coli content In meat or Meat product??

[@MicroChemsExperiments]

Yes. You can use this method for meat and meat product.

[@BhavyaRecordsOfficial]

Where u do job?

[@MicroChemsExperiments]

@@BhavyaRecordsOfficial The question is not related to the video.

[@zarqaarshad411]

Kindly you tell me which exact color of E.coli appear on chromocult agr

[@MicroChemsExperiments]

It is already shown in this video. Dark purple to blue

[@bhargovmudhukailya4727]

Can i have any research paper following this procedure

[@MicroChemsExperiments]

Yes. You can use this method in your publications. Because this method is strictly followed by ISO method.

[@bhargovmudhukailya4727]

Thank you

[@MicroChemsExperiments]

You are most welcome

[@zarqaarshad411]

We can not incubate sample after adding it into peptone salt

[@MicroChemsExperiments]

Yes

[@lithupaul5316]

Can you please do videos for enumeration and detection of salmonella spp, vibrios spp, staphylococcus spp, clostridium spp, listeria spp .. it will be helpful

[@MicroChemsExperiments]

Lithu Paul. We already started working on some of your mentioned bacteria for making videos. We will upload soon. Be with us.