Total Yeast & Mold Count (Total Fungal Count)_A Complete Procedure (BAM, Ch-18)

[@sonandpets1215]

I haven't received notifications for your posts in a while. I missed you, your team, and your videos! Thank you! Welcome back!

[@MicroChemsExperiments]

So glad to hear this. Actually we were so busy in our professional life. Now, we are back. Stay with us.

[@ammardilawar510]

Thanks for the excellent video. I have few questions 1. What can we use instead of stomacher bags ? 2. How to prepare sample if we have swab samples or environmental samples ? 3. Formula if we have n3 or n4 also ?

[@MicroChemsExperiments]

1. Large duran bottle 2. Dissolve the swab stick in 10ml buffer solution. After shaking and vortex, dilute if necessary. Then analyze. 3. First calculate average from 2 plates. In this way, make 2 values from 4 plates.

[@christopherkambondo4806]

this I waited for long, thanks

[@MicroChemsExperiments]

I made it for you

[@md.shahinsarkar1625]

Good video

[@sujonali7125]

Thanks for procedure

[@karthikpharmacist5271]

Nice teaching mam

[@MicroChemsExperiments]

It's my pleasure

[@shubhamsorate8918]

Excellet video

[@MicroChemsExperiments]

Thank you very much!

[@RubenNdosiba]

merci pour la video

[@manarasmr6946]

Well explained thanks

[@queeniedalisay9970]

Hi, is okay to use potato dextrose agar? with same procedure?

[@kelvindanntobias5642]

Yes.

[@jkgou1]

Would you please instruct us: The sample on each plate is 100μl(0.1ml) Do we need to multiply the final result with 10 for 1ml sample for this spread plate method ? We use 1 ml sample on each plate for pour plate method but we use 0.1 ml sample on each plate for spread plate method.

[@davoudsalehi8386]

Sehr gut 👍

[@mohdabrar2839]

thanks

[@MicroChemsExperiments]

You are welcome

[@santoshrock1943]

Can u please tell ........What is the formula for calculation if colony count in one successive dilution is less than 150?

[@srikanthsri9442]

Kindly upload the Enumeration technique of Bacillus Coagulans

[@MicroChemsExperiments]

Noted

[@sibamdatta9723]

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

[@dennarkoe5928]

26 degree for mould yeast with slowly incubation 5 day

[@firasabili5142]

Is this method same with ISO 16212:2017?

[@dessienatalia1193]

Hello. Does pour plating method can be used for yeast and mold culture?

[@MicroChemsExperiments]

In that case you have to use SDA media for pour plate

[@soulrelaxation5977]

You said now we isolate bacteria?

[@ruqiyakhamroon4434]

Can we use potato Dextrose Agar instead of DRBG Agar

[@MicroChemsExperiments]

Yes you can but you need to add chloramphenicol as a supplement to make it more selective for Yeasts & Molds.

[@ruqiyakhamroon4434]

R can we use potato Dextrose Agar w/chloramphenicol

[@Ninxh678]

Hi! Great video. Just want to ask did the sample weight change from 25g to 50g?

[@MicroChemsExperiments]

The question is not clear. We took 25g sample for testing

[@Ninxh678]

@@MicroChemsExperiments because according to the FDA-BAM Chp 1 the sample weight stated to be 50g. So I just want to ask did the result for 25g different from 50g?

[@MicroChemsExperiments]

@@Ninxh678 Result will not differ.

[@Ninxh678]

@@MicroChemsExperiments Thank you!

[@justacommonman7000]

What is meant by BAM in the title?

[@MicroChemsExperiments]

Bacteriological Analytical Manual

[@rakeshbodhe8460]

Please do the video of residue free chlorine

[@MicroChemsExperiments]

Noted

[@manojpheiroijam]

Please upload video for Staphylococcus aureus and Enterobacteriaceae count

[@MicroChemsExperiments]

Noted

[@olabisiolatundun3696]

Please can I use thesame technique and culture Media for pharmaceutical samples?

[@MicroChemsExperiments]

Yes. You can

[@Haokip_Lunjapikai]

What are the other media that can use other than DRBC agar??

[@MicroChemsExperiments]

You can also use "Yeast Extract Chloramphenicol Agar"

[@Haokip_Lunjapikai]

@@MicroChemsExperiments thank you

[@Haokip_Lunjapikai]

Can we use other media like Potato dextrose agar (PDA), Yeast extract Calcium carbonate agar (YECA) and Sabouraud dextrose agar (SDA)?

[@neethuaneethua941]

Can we use Butterfield phospate buffer instead of peptone water

[@MicroChemsExperiments]

Yes you can

[@neethuaneethua941]

​@@MicroChemsExperiments thank you ❤

[@mumuhay2345]

is there any video for bacterial count?

[@MicroChemsExperiments]

Yes. Link: kzbin.info/www/bejne/f523n397nM6pqtU

[@sona.r1662]

Why we use diluent instead of water

[@julytran861]

👍

[@MicroChemsExperiments]

Thanks

[@gousiagulzar7002]

Can we use blending jar instead of stomacher machine

[@MicroChemsExperiments]

Contamination can occur in blender jar. So you can shake by your hand....or you can use vortex mixer

[@gousiagulzar7002]

@@MicroChemsExperiments thank you

[@MicroChemsExperiments]

@@gousiagulzar7002 welcome

[@arlhenpinera1884]

Do you still need to add antibiotic solution in the media?

[@MicroChemsExperiments]

No. No need to add antibiotics in DRBC agar media

[@arlhenpinera1884]

@@MicroChemsExperiments thank youu

[@sambathsocheata1389]

Can we use this method for determination of yeast and mold?

[@MicroChemsExperiments]

Yes

[@sambathsocheata1389]

For enumeration need 6 falcon tube then how about determination do we still use 6 falcon tube or just use 2 tube only ?

[@MicroChemsExperiments]

@@sambathsocheata1389 you can use only 2 tubes. It depends on the expected concentration of yeast and mold in your sample

[@sambathsocheata1389]

Thank you ❤

[@nainamohamed6727]

Is there any chemicals to prevent yeast and mould in packeged drinking water

[@MicroChemsExperiments]

Yes. benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate are used to prevent the growth of yeast and mould

[@willdrin2851]

Can you use buffered peptone water instead of 0.1% peptone water?

[@MicroChemsExperiments]

You can

[@ayakhaled2474]

Incubate plates in upright or invert position

[@MicroChemsExperiments]

Invert

[@ayakhaled2474]

But in iso method 21527-2 is written: 9.2.3 Incubate the prepared plates aerobically, lids uppermost, in an upright position in the incubator at 25 °C ± 1 °C for 5 d to 7 d. If necessary, leave the agar plates to stand in diffuse daylight for 1 d to 2 d.

[@nainamohamed6727]

How to control yeast and mould in packeged drinking water

[@MicroChemsExperiments]

Add one of these chemicals benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate.

[@nainamohamed6727]

@@MicroChemsExperiments thank you for your kind reply one more doubt sir how much qty add per litre sir

[@sorngsinadane8447]

Excuse me, can I get the reference of calculating formula?

[@MicroChemsExperiments]

Reference is given in the video title

[@sorngsinadane8447]

@@MicroChemsExperiments Many thanks to you. Keep this good work always!☺️

[@MicroChemsExperiments]

@@sorngsinadane8447 Thank you so much.

[@m.luqmanulhakim2672]

what is the different about bacteria and yeast in Macroscopic ? I am still confused. Thank you

[@MicroChemsExperiments]

Study more in Google resources

[@QualityControlLab-d6s]

one more issue media are not solidified

[@sorngsinadane8447]

Hi, I am wondering what is the purpose of labeling 2 trial plats? eg: DRBC10-2 / trail -1 and DRBC10-2 /trail -2. Thanks in advance.

[@MicroChemsExperiments]

Contamination can occur in every steps of any microbiological test. So double trial can increase your confidence level of the test.

[@ruqiyakhamroon4434]

I haven't received any reply

[@MicroChemsExperiments]

Sorry for late reply

[@sibamdatta9723]

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

[@sibamdatta9723]

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

[@Mmababy24]

37

[@sibamdatta9723]

Which temperature to set incubator 22-25° or 37° plzzz reply 😢