Eva Nogales (UC Berkeley): Introduction to Electron Microscopy

[@MirzoKanoatov]

This has answered all of the questions I had about cryo-EM! Thank you!

[@TheGreenPastures]

Thank you! Very clear, detail and very well explained. Very good teacher. Lovely !

[@rafaelmarquesdasilva948]

Thanks Eva. I am learning Cryo EM now and it was really curious when you mentioned about septins in yeast. I was the first person to solve the structures of yeast septins at high resolution. Nice how we are connected by coincidence.

[@sarahmushroomkiller9233]

Thank you, Eva! Great introduction! Very helpful!

[@qammerzaib]

Thanks Dr Nogales. It is very helpful.

[@premashismanna1989]

very useful..thanks a lot Eva..

[@VictorAndScience]

This is amazingly explained. Thank you Dr.

[@robinbreslin1626]

At about 7 mins in, that is not an X-Ray image. It's a Nulcear Medicine bone scan.

[@anonviewerciv]

5:30 Electron interactions. 25:00 Sample types.

[@TheSiliconchip]

Nice and informative. especially for biological samples.Super.

[@chantellehollingsworthplow4695]

Opened my mind into more fascination with EM

[@RoyceBarber]

1. Her presentation is top notch. She speaks clearly and intentional, boldly and confident. She's articulate and logical. 2. She's extremely intelligent. 3. I'm very much attracted to her, I can't hide that! Perhaps some day I'll marry a TEM CEM Electron Microscopy professional.

[@jacklane6483]

Thanks for posting this video!

[@mikaelfalk6720]

Love her work on tubulin :)

[@milly229]

introduction was really great, helped a lot for studying, thank you

[@skmolugu]

Worth wathing this video...! Thnx much for posting it,..!

[@sabaimdad4998]

Very well explained. Thank you Eva

[@DaiMingTang]

beautiful video, very helpful. Thank you very much.

[@nisalisan]

This is such a good lecture video on this topic! Thank you!

[@buddesatva]

Really excellent video.

[@kentheengineer592]

great information please provide your references for how you discovered these idea's

[@haidermuhamed8022]

thank you so much Eva

[@arnizaghazali6762]

When an expert in a field run analysis from sample prep to image rendition!

[@candocoaching8381]

thank you very much great explenation!

[@sethkostek]

Eva you Rock!

[@robinbreslin1626]

Amazingly instructive video though.

[@derrald]

WOOHOO, ELECTRON MICROSCOPY! GO SCIENCE! ROCK ON!

[@edwindeleon5232]

Need EM test to get diagnosed with hiv its microscopic.

[@edwindeleon1130]

it can only be seen through electron microscope hiv it microscopic

[@jogitomonva]

good video and buen acento...

[@mohamedchanfioumkouboi6443]

very thankful

[@josezavala8270]

Great... thanks!

[@kamldeepyadav6232]

Thank you

[@joncoteeglise4252]

Fantástico!

[@edwindeleon1130]

necessity mother of invention

[@classica1fungus]

She is 🔥

[@libertario1277]

Very good video gracias. You´re pretty

[@edwindeleon1130]

for hiv

[@imbis253fois]

Cheers

[@mentalerik9008]

Hot

[@vorman4]

YEAH! SCIENCE BITCH!

[@craftye2429]

Slightly annoyed by the use of metaphors and personification, rather than explaining some things scientifically, however, it was generally a good video.

[@jacquieliddell2536]

Hot chick doing the science...

[@rayr1444]

One thing caught my attention on the cryo electron microscopy method... You say, fast freezing, a million degrees per second. Really? Think about that and then please define your meaning of the word degrees.

[@2010Manusha]

It is not about defining degrees. Your fallacy is probably that you think of the temperature scale and a million degrees and wonder how that fits in. However, the sample goes from ambient to liquid nitrogen temperature, i.e., from around 20 C to -196 C. That is a drop in -216 C. Now if that temperature drop happens in a time frame of a couple of hundreds of nanoseconds, that is a cooling RATE of a million degrees per second, which neither implies that your sample was acquired from the surface of the sun nor that it is cooled to temperatures where even hell freezes, i.e., below absolute zero at -273.15 C, but only that heat exchange happens extremely fast.