ImageJ Analysis: Length Measurement, Area Measurement and Thresholding

In this ImageJ tutorial basic analysis of any image like length and area measurement are demonstrated both by manual and thresholding process.
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Instructor: Santanu Mandal
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[@baikibadai]

Thank you so much from Buenos Aires, Argentina. My students and I were watching your video. The cadence of your voice is perfect for people who is not a native English speakers. Keep doing videos for research community.

[@SMSTechEdu]

You are most welcome :)

[@anna-ne9zk]

I am using Image J to analyze herbivory in my honours thesis, and this tutorial really helped me understand how to use it! You are a life saver, thank you!

[@VirtualSolace]

I am so delighted to watch this video. Now my problem is solved🙏🙏

[@kalpanar5896]

Thanks a lot. Was exactly what i was looking for. I had no idea how to start using it but this video had all the info i needed and i was able to tweak the steps for my study. Lifesaving! So grateful😄😄

[@Dankhard_Schmidt]

Thanks for the tutorial, has now allowed me to analyse bubbles in a foam, the threshold is very difficult but your demonstration on how to do it manually has helped

[@bavyadevi1349]

thanks for the team who made this video in a fruitful way. come up with many more useful videos for the research community. All the best for SMS team.

[@SMSTechEdu]

Thank you so much for encouraging words!!!

[@rmg7298]

Thank you so much for this video! It's hard to find straightforward explanations for ImageJ.

[@angelicaclaus]

Thanks for the good work. It's my first time trying these analysis, videos just like yours helps a lot!

[@utkarshmisra7611]

Thanks for the tutorial. It is a life and time saver. Kudos 🎉

[@BBlueFlores27Y]

Thanks from Mexico. It really helped me a lot. It's clear and useful for beginners in this software

[@Anatomy.doctor]

Thank you so much sir 💐 for making this so simple for me... You are a great teacher.... ❤

[@gargivaidya3099]

I was searching for such calibration since many days.. Thank you so much you solved my query!!

[@karenwells1604]

This video was super helpful. I'm trying to determine if ImageJ will work for my project and it seems that it will. Thanks for making this video.

[@johnziyadeh5489]

Thanks for your video from Beirut, Lebanon!

[@Kush-v3l]

very informative video, thanks a lot for such an easy way explanation

[@suleimanbashiradamu]

Very rich presentation for me as a beginner. Thank you very much

[@claraconstanza9321]

So helpful!! Thank you so much! I could easily apply the methods on my data and had great effects, the video was an amazing help! :)

[@markopapp1777]

best vidio ind the word my best youtuber :D

[@rahmetm2784]

very helpfull, thanks too much

[@patcharawansujayanont1761]

Thank you very much. This video saves me a lot.

[@mhasan-vd3vr]

Helpful. pretty nice presentation

[@rubinaajdary3349]

BIG BIG thanks for the great video!!!

[@helent.862]

This is very helpful! Thanks for your video!!

[@SMSTechEdu]

Glad it was helpful!

[@arpita8416]

​@@SMSTechEduhey , I am not able to do it manually while calculating area like I have selected the area and after clicking analyize,there is nothing

[@winks2910]

Thank You for this. Very helpful.

[@farahmirzoyeva9674]

Thank you very much! Super helpful!

[@amarariaz992]

This video is really helpful. I have a request, plz guide, can we automatically measure cross-sectional area for different colored particles in one image in a way it can detect, measure and enlist all the particles i.e. their number, their respective areas instead of considering them same kind of particles?

[@bhagathyb9920]

Thank you sir, very useful video.

[@sharmilag3061]

Explanation super sir

[@prashantkumarchoudhary9744]

Nicely explained

[@ergobibamus4409]

Awesome, thank you so much.

[@ghozinashiruddin2414]

Thanks a lot for your explanation Sir

[@muhammadwaqasluqman2977]

Thank you so Much for This.

[@Commando303X]

I wish the audio were louder, and I wish you spoke faster. Other than this, an excellent video, and I thank you for sharing it.

[@hongshen903]

Thank you for your video and tutorial! Very friendly for new users!

[@Seyanhyriu]

Thank you for your help. Very slow and well explained features. Thanks again.

[@maramarchetti9552]

Thank you very much, your work is so precious...

[@yoganurwijaya5877]

thanks for all of your videos, very good explanation, slow but you have explained all the details :)

[@memoghal]

Thank you. really helpful tutorial

[@FajrulF]

Thank you from Indonesia. This video help me a lot!

[@SamSam-jr5qu]

Great video....it's really helpful

[@arismulyana9758]

Thank you for your video.. Very usefull for me

[@OthmanAlikhan]

Thanks for the video =)

[@pradipbhattacharyya3968]

Awesome video. Very helpful.

[@Anilkumar-yd5dx]

Very useful, Thanks for providing such things

[@颜十二]

thanks for your contribution

[@brianmakuza2953]

Thanks for the video

[@lalitha55555]

Thank you very much.I could learn plotting histograms using this video

[@PatriciaNerydesiqueira]

Hiii, I hope you are well :) Do you know of any alternative to using Threshold in macro, so that it automatically matches the result of each image? Because each image generates different values, and there is no way I can provide a fixed value that works for the entire set of images (there are many)

[@SAURABHMANDAL]

Thank you santanu mandal!!! From, Saurabh Mandal😊

[@m3lisa15]

if my images was more than 100 can i measure the length and width automatically instead of select the images manual?

[@haleemasadia2787]

please upload more videos regarding to foliar anatomy.... (cells length and width measurement)

[@ajayhathur]

Great video!!! Thank you for the effort.

[@merveemen3838]

Thank you so much for this informative video. I would like to ask Is the threshold be calculated separately for each sample? So, the values ​​set at the threshold do not need to be the same in every example, right? Thank you so much.

[@SMSTechEdu]

You ae right. The threshold need not be the same in every sample.

[@chenerrera9158]

Great video! thank u. Quick question though- what are the units for the area? if I clibrated a scale of micrometers, than is is um^2 or some other defalt units?

[@pandaramperumal7010]

Thank you very much Sir. It is very nice and informative lecture for the learners.

[@karthikaguna2431]

Thanks. What is the area value showing in output when we measure the distance between two particles. Highly appreciated for your help.

[@unnamed1125]

Thank you sir. Really appreciating work.

[@bishnuyadav273]

nice lecture..pleas upload more videas regarding image J

[@raqibahsan8186]

Thank you very much for this tutorial 🙂

[@vgyan4u525]

its really helpful sir thanks a lot

[@shazlir5415]

Learned alot. By any change do you know how to calculate collagen fragmentation index of superficial dermis?

[@TobiasDettinger]

Thank you for introducing this cool software. How to draw not a straight line but a curve? I have fibres i want to measure the lenght of but a line is not the tool i need for this.

[@TikTokTrendsPinas]

Thank you very much

[@cocowang3227]

Thank you very much! It is pretty helpful for freshmen. May I ask a question? Why it is required converting to 8-bit before using image?

[@apinyaraksa6860]

Thank you for your sharing

[@JBoardOnly]

SMS, we need to determine the area of a JPEG image and then within that area an anomaly.... that way we can compute the percent that the anomaly is of the total area. Do you recommend ImageJ or jiji ImageJ? We cant calibrate or determine the area of size within the image, that is why we need to determine the percent. The 1st area is essentially a circle the 2nd inside area is somewhat of a triangle. Thanks in advance.

[@sumitsharma9187]

Simple and awesome......:)

[@deepaknegi6126]

Thank you Sir for the video

[@jaquelinevazquez5325]

Excelente. Muchas gracias.

[@loucouoh]

So helpful!! Thanks so much!!

[@shanmugapriyaig8521]

sir, i have 2 questions. 1. why you convert image to 8 bit? 2.after measuring length of the particle, i want to write text in the image. i used A symbol and i write length unit. once i write i cant edit. can you help me about this

[@pianickname8514]

This is a very good and helpful video. Thanks! Also I noticed you are recording this at 1am :D

[@mwdasja1127]

Thanks for the video, but need sound improvement please..

[@UziTryHard]

thank you sir for your video

[@DOMIN8RRR]

Very useful

[@dularmiru]

Sir, could you help me, how can I measure 0.0625 sq. mm area on an image by using 0.25 mm scale bar. Thank you

[@devasritadash3590]

Hi, this is very helpful and easy to follow. Thank you very much. Is there a way to measure the selected rectangular area so that the same area of interest can be kept uniform for multiple images? or if we select the rectangular area in one image and are analyzing a stack of images, will that same rectangular area selection will apply to all?

[@arghaghosh4113]

Hello, I had the same question as that of yours but unfortunately, I was unable to find any videos regarding having a constant rectangular area and using it for all analysis. Please let me know if you figured out a way. It would help me greatly! Thanks!

[@yousefdehghani2050]

so helpful, ty.

[@abdulsal]

I dont have the scale value when i open my picture like in your case (300nm). what should i do?

[@Mo.w77]

Abdul Salam you can use an image measured from the same camera and magnification to set a scale. In biology people often use hemocytometer to capture an image and set a scale

[@bigyanpanda1]

how to get a scale bar for any digital clinical photo of facial structures.

[@maheshbiradar2510]

Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ?

[@leixiao169]

great video! why you need to convert your image to 8bit?

[@TheArmedwithWings]

thx for the informative vid. although there were times where i had to rerun with your accent a bit thick at times. perhaps add subtitles for future vids?

[@abdelkaderazou3134]

Thank you for explain but I have question about thresholding with ImageJ...how I can devise histogramm of grey scale image in different ranges from 0 to 255 for your image J for exemple.. Thank you

[@fang-djamal]

Is there anyway to automate finding distance between points? I am trying to find the distance between neighbor atoms in a lattice from a TEM image but it would be cumbersome to do it one by one.

[@nazatulain3211]

hi may I know where is the next video on threshold in image j?

[@jeansalazar9685]

Hi professor. Great video! I will appreciate so much if you can clarify my doubt, it is about the result of the mean value obtained by the thresholding method. The summarized results showed 2851.485nm^2 +/- 1217nm^2. So, is it possible to say that the grain size of your sample is: 2851.485^0.5 +1217^0.5 = 53.40 nm +/- 34.89 nm ?? Thanks for your time

[@albo5194]

that would obviously depend on the shape you want to assume. Most particles shapes are approached using spheres so you would need to find the diameter of a sphere with given cross sectional area.. if the shape is very irregular i would either take an average of different lines through the particle or choose a strategy (like e.g. finding the maximum Feret diameter)

[@Venom8040]

your Video helped me a lot thank you but plz try to make your voice more clearly =)

[@vireshkumar9241]

wow sir .....very easy to use ImajeJ after your tutorial.......one question sir can we get the composition of composite materials (eg: Nickel Ferrite and carbon) from ImageJ?

[@SMSTechEdu]

Thank you. You have to do XRD/EDX for composition analysis. Using ImageJ ,we don't think its possible!!

[@vireshkumar9241]

Thank u sir

[@tanjirahmed3046]

Thanks. But what area is measured when you measured the inter-particles distances?

[@SMSTechEdu]

There is no area. It is a linear distance.

[@aishaz5488]

I have question ...if our image has no scale it's taken from smart phone then how it's work ?

[@SMSTechEdu]

For making length and area measurements, a scale is mandatorily needed with the image.

[@Harsh.Parekh]

Why do you convert image into 8bit mode? What happens if you retain RGB mode? Thanks

[@fikriirsyad2223]

Why did we have to change to type to 8-bit for measuring?

[@oscarnoe3953]

Perhaps it is easier to process the images with this configuration, in addition to its lower weight.

[@denisedemurtas3985]

Thank you very much, for your tutorial. I was wondering how can use the threshold if I have holes onto a surface area that I want to measure? It feels like they break my total area in small pieces. I was thinking to take about 5 measurement of it manually at this point and use a standard deviation, could that work?

[@SMSTechEdu]

Yes. It should work perfectly.

[@rashmishivananda1704]

Thank you for the video. I am using Image J for analysing my bubbles (from Foam). Since the bubbles are see through, I need to use the manual method for selecting the bubbles. However , this is taking a lot of time and some cases it considers one big bubble as two and calculates the area. What is the solution for this? Though I set the scale initially to cm, it displays results in area percentage. In this case what would be the unit of my results? Please help.

[@marouanesh9566]

hey i have also the same situation. did you solve this problem?

[@samanjaved8983]

hi, how first scale can be set in the pic. as in your example the image already show the scale of 300nm in length, how this scale can be set.. kindly resolve that problem

[@SMSTechEdu]

That is the scale bar which you have to obtain directly from microscope using any image capturing software like LEICA IMAGE ANALYSER.

[@joschateichmann1065]

Nice video, more fps would be great though.

[@IzraSunshine]

Why do we need to convert the image to an 8-bit mode? Is there any specific reason for this?

[@AliceritaE]

conversion to 8-bit improves the quantification because it converts the pixel into binary i.e white or black

[@Ulfguis]

hello, why do we have to convert the image into 8bit? just curios

[@marriamyamin3766]

can we use imageJ for measurement of diameter of cells e.g. bacterial cells from TEM images??