Follow along as we show how to sterilize grain mushroom growing substrate using a pressure cooker.

[@silverleapers]

Andrew, in addition to venting for 7-15 minutes after full boil, you should be venting the autoclave after it builds up pressure (read the All American manual, or even better do an accredited autoclave course on-line). This second venting will allow trapped air to be expelled from inside the bags and allow the hot steam to enter the bags. It will reduce your contams. Hope this helps. Good luck with the new bigger factory!

[@godzillal123321]

The quick release of the pressure may burst the bag

[@silverleapers]

@@godzillal123321 It is only a short release time. 1-3 seconds usually. Its a small pressure drop, not a huge drop. It's to get the air movement ie bags to breathe.

[@skinnywheelz]

I normally lerk around, hardly subscribing or 👍. I have watched many of your videos and I appreciate what you do. So today is the day I like and subscribe. Great video, Thanks for sharing!

[@alexandrevaliquette1941]

Please, just accept free love from a random dude in Montréal. You have outstanding tips on mycology. Very inspiring, thank you so much for sharing for FREE.

[@Mossycreekmushroomsofficial]

Thanks! Love to hear it was helpful! That's our goal!

[@Aztecorum16]

The music is fire in this video! 🔥🔥🔥

[@Mossycreekmushroomsofficial]

Thanks!

[@shellytanner4727]

Bassnectar??

[@good4u88us]

I always put a plate across the top of the grain bags in my Presto. I've heard the grain bags can open and block the pressure release valve and cause trouble.

[@antonbackstrand]

I Always do this aswell. Please everyone do this aswell and you should to Mossy. Please do some research into it. Much love

[@NedHooton]

I had this happen to me. Giant fountain of substrate/water geysered from my cooker. It was terrifying and no fun to clean. What type of plate do you use to cover your bags?

[@NedHooton]

Also curious if you are facing the concave side of the plate towards the lid of the PC or away from it?

[@Mailmartinviljoen]

The high pressure and possibility of the pot exploding is not the only issue . Another problem comes in when you think all of the steam is out when in fact it's not. I didn't have mushroom bags so I experimented with normal bags. When my 90min cooking was done I manually vented by lifting the weight and only a little bit of steam came out causing me to think its safe to open. Little did I know the bag clogged the valve. When I opened it all of the steam cam rushing out. Fortunately no burns!

[@nickkempel6540]

"cause trouble"

[@romainarnoux]

I use to sterilize 90min @ 15psi and I was having some molds. Thanks to you it seems it was not enough! You just make my day sir with this cristal clear tutorial. thanks for that! Romain from France

[@cameronferguson8016]

Andrew and the Mossback gang your back! Thank goodness, I was getting worried. Does my heart good to see you back.

[@Mossycreekmushroomsofficial]

Thanks, it means a lot. Sure has been busy around here lately! I'm doing a live soon!

[@Paul-ke5lu]

What is the water level in relation to the filter patch? I assume the water is below the patch. In your video you pointed to the water level on the side of the bag but when you did this the bag wast turned around (filter patch o other side). When I pressure cook in the fashion your demonstrated I end up with condensation in the bag, and Im concerned about getting a good seal with condensation inside the bag. Do you end up condensation in the bags? How can I prevent this? BTW great video keep up the good work!!

[@vk081064]

That's a nice informational post. What pressure and time duration would you advice for millets sterilization. Thanks

[@glennluarez6159]

Glad to see another video .... wish i had that All American Autoclave..... Greetings from the Philippines

[@Mossycreekmushroomsofficial]

It is fantastic! Thanks for watching, Glad to have you!

[@MissBlackMetal]

Love the info -- and the Mjölnir necklace! 🤘🏻

[@authenticintel]

I Love the Myco community and collective knowledge being shared. Thank you to all the contributors like yourself. I am considering this tek for sterilizing a CVG substrate bag for the hoodie tek taught by practical mycology. Would you suggest any changes to the tek for a CVG bag that I will be adding colonized spawn into for an all in one bag-ish?

[@tao76]

Did it work out?

[@patrickanders6776]

What a BOSS!!!! nice Thor's hammer! Great vid, just the help I needed!

[@paulamerson7581]

Does the filter patch get wet in the presto? If so, does it not compromise the efficacy of the filter?

[@jennybell6103]

Thanks so much. - First time for me trying to sterilize my sawdust blocks in a pressure cooker. Feeling a bit nervous - but more confident now after watching your video :-)

[@toastybrown]

Hi, Thank you for the videos! So it's ok for your bags to touch the side of your press pressure cooker?

[@RichardSimons-gy8xu]

Hey buddy. Love your channel. I am about to use my Presto 23-quart to sterilize substate and I had a couple questions for you. You instruct to put the opening of the bag to the top so water does not siphon back into the bags, but you don't mention the filter patch, which appears wold be below the water level that you indicate. Does it not siphon back through that? Also, is the extra water beyond the three quarts recommended by the manufacturer to keep it from running out when you run it for two plus hours? Thanks, Richard

[@Purplebb676]

Great video helps a lot this is going on my Christmas list lol I have hit an age where games don’t matter and I need to make money just have to wait for winter to be over

[@kicknadeadcat]

This is my first go, oyster mushrooms. My block stalled out. It’s been a month, it’s 2/3 colonized and been that way from the second week. Should I just fruit what’s there?

[@RipperYouTubeOfficial]

Can you link the heater you use for that presto?

[@ralphmalph6824]

Do you do the prep venting and releasing as mentioned in All American instructions? Thanks for the video

[@thebluetravelers7544]

Glad to see another video posted! I'm working on substrate sterilizers can you do a video on boilers trough system and the parts you use i need a good float valve 2nd time now my drum got filled with water!! Im bought to make a boiler because of

[@jrcicirello]

You might need to regulate the water pressure so your float valve works correctly. I DIY'd a steam sterilizer drum like the ones over on Bubba's Barrels. I bought the sanitary stainless steel drum from Carl (owner) and he gave me tips about how to DIY the sterilizer. The float valves work best at 5 psi to 15 psi. Get a good regulator and play with the valve and regulator to see what pressure it completely stops the flow. I hope my experience helps. Good luck and let us know how that worked out for you.

[@thebluetravelers7544]

@@jrcicirello thanks for the advice but thats what I just figured out also I ordered one and set it to 10 psi but it overflowed again maybe float valve got touched and I'm just so tired of dealing with it in gonna build a boiler so I dont loose anymore batches and I'm also scaling up my operation and want to do the trough sterilizer next

[@jonathanhella7194]

Great video man...keep at it!

[@zacbarbour5235]

so i assume submerging the filter patch in water doesnt have any negative effects?

[@jasonblunden6472]

Been watching your videos for a while [from the days before liquid spawn] - goofy smiley. Anyway as always a good video, thanks for sharing. Kudos to you, and keep up the good work. PS this worked excellent for me - thanks for the 'self-sealing' tip. Suppose logical in retrospect, but now can 'vent' my PC without bags blowing up/distorting.

[@humanearthlingthing]

Thanks Andrew, great stuff,...does any water ever get in through the filter patches in the smaller unit??

[@redfoot7951]

Same question.

[@jrcicirello]

If any it is minimal. I never noticed any additional water because of the filter. Usually what happens is the air pressure in the bag is always trying to escape causing an over pressure effect that would displace water attempting to get in. Personal experience has provided a wealth of information. If I had thought about how to set the bags in upside down, I would not have ruined seed spawn as well as grow medium my first few runs.

[@sevensurvival]

Thanks Brother. I appreciate all the good that You do a lot. Respect

[@Mossycreekmushroomsofficial]

I appreciate that

[@warlock1005]

Do you release the pressure or just let it cool naturally?

[@JohnLopez-h2u]

Used the presto 23 to do masters mix. Followed the instructions 15 psi ran it at low flame for 2.5 hr. Bags over cooked. And cracked , I rebaged the sterile substrate . ( is that a no no) using 5 lb bags seems to over cook ? Any suggestions

[@redrockpiercing]

Perfect video for today, Ill get my Presto 23 qt pc today and I wasn't sure about the water level on and around my bags, so thanks a million. BTW, I loved the video on how to make LC lids, I even ordered some(how's that coming along?) , I can't wait to start some LC. I wanted to ask, does the syringe filter have to be PTFE or will Nylon work in the pc as well?

[@articulatingprimate6980]

I put in some 2 1/2 inch stainless steel screws to prop up my plate on the bottom so they aren't sitting in the water. I've found for a 2 1/2-3hr cook it's just the fight amt of water. Leaves about 1/2 of water left in bottom. This is in a presto 23 or 28qt PC.

[@fishmut]

First time using my pot today , had the same amount of water and it was nearly bone dry on a 2 hr 15 psi . I’ll be putting about 3 to 31/2 inches to make sure I don’t wreck the pot or my grains , I found backing off the temperature the psi dropped so I think the steam counter weight is not right , thinking it should be a tiny bit heavier , old thread I know had to share it.

[@daphneraven6745]

Sir, I’m a little astonished that your bags don’t melt in that kind of heat for that length of time. Delighted about it, of course, but wondering what grade or type of bags a person needs to use for this.

[@slowpoke2372]

You said less time for rye, like how less?

[@waaaahooooo1538]

Andrew how much water should I add to my 41qt sterilizer for a 4 hour cook (for production blocks 5 10pounds bags)

[@MycoManMike]

u using grain and not hard wood pellets or brikets for substrate? and how heavy u fill your bags with substrate pls? ty so much

[@BiggDaddie008]

Great info Thank You. What size bags are they?

[@Avintrue]

You said that you would back down the time for softer grains. What would you recommend for the length of time for sterilizing rye grain? I have burnt some doing the 2.5 hrs.

[@spikespiegel2537]

I see a lot of people that make a fold at the top of the bag about 2inches down after the fold the bag down, it is intended to make a seal at the top of the bag. I notice you dont fold the top after pressing the air out. Is the extra fold on the top to seal it not necessary for grain and sub bags?

[@pny2579]

Hi there. I am just wondering what is the difference between the all american canner vs the all american autoclave? I've order the pressure canner and then realize there is also a All American autoclave. I'm confused. Thanks

[@rizgarj8050]

Hello dear Could you please advise me on the dimensions for the racks and distance between them? Thanks Rizgar...

[@prateeksharma2751]

Thanks! Why do you leave the pressure cooker overnight before opening it?

[@sirDrgreenSlick]

Thank you very much for your informative videos and generous hospitality... I would like to ask if you can make a video on substrate you use and other nutritional things we could add like ground oyster shells for example coffee ...ect if you can get around to this comment I know times r busy and lots to do but thank you very much we appreciate you

[@jensraberg8620]

Would it be good to have some small spacer between bags for the heat to permeate better?

[@dennisdicicco6406]

I bought some medicinal LCs from you. Do you know how to do a mycelium extraction?

[@BoroBootBoy]

Hi there, just wondering what you use to heat the Presto 23 quart? My cooker hobs are too small (UK sizes!) to use so was looking for a single hob unit, but again they are all too small!

[@nickkempel6540]

Is that 1,300w hot plate enough to run the presto 23? I have 4 and really wanna switch to electric is that hot plate a good option?

[@Jkfrasier1980]

Shit I’m not at this level yet. I’m growing my first at home grow bags and a little monotube at this moment. Guess I’ll come back when I get to that stage of this. Thank you sir

[@85mmonroe]

How is the tolerance on your All American thermostat? I've been running a 25x and it has a 4-5psi window. Best I can get it to run is 15psi-19psi. Do you think the pressure fluctuations will be ok?

[@sunnyjones682]

Is the filter outside or folded inside of the fold?

[@robertevans2648]

How do you get stump pumps to shut off your Mylian to another syringe?

[@Mewtwo420]

Do you have any good bag recommendations?

[@alice_orcmommy]

same for sterilizing in jars???

[@Shuniatarg]

Hey Mossy! Did you sterilize the oats for 2.30hs? It's not much? I thought 1.30hs 15 psi would be enough! Thanks x answer !! Big up!!

[@TantricBioHacker]

Do you have to steralise homemade substrate? Isn't the mycelium strong enough from the agar petri dishes to with stand any contamination at this stage?

[@Mossycreekmushroomsofficial]

Mycelium moves slower than bacteria or molds. The best way to get good growth is really to give the mycelium a head start by sterilizing the substrate. There are selective substrates which can be treated differently or even left untreated.

[@TantricBioHacker]

​@@Mossycreekmushroomsofficial Thanks for your reply so even by going to Agar petridish to grain bag to letting mycelieum spawn fully in a full grain bag and then mixing it out with untreated coconut-fiber it still risks messing up?

[@CriticoolHit]

What's the name of that dank jam playing during loading the pressure cooker?

[@jake_runs_the_world]

Hey, could you please tell mw what the insert is called? because im having a hard time finding one

[@mszombiekilla19]

Thanks for the video! What are the names of the bags you use ?

[@zachbojorquez5286]

Does this apply to gusseted and non gusseted bags forming a vacume seal?

[@jammyfindlater]

Could you help me please. I just wanted to know whether a bulk atmospheric sterilizer is sufficient for sterilizing grain spawn? Or can you only use them to sterilize bulk substrate?

[@discusctx]

Ok. So after I estrilze the blocks. Then u inject the blocks with liquid cultivation. Then wait. My question is do I use that same block to grow my mushrooms or do I transfer the sawdust (or what ever) to another bag for growing.

[@xxvietblood]

Hey there great video! Noobie question, are we sealing the substrate bags before pressure cooking?

[@tao76]

no

[@bitbybit1231]

New to sterilizing bags here, i did my first 4 bag batch yesterday and the 2 bottom bags stuck and ripped. The top 2 were find but plastic was quite crinkled. Any idea what went wrong? I vented 10 minutes and added about the same water shown in this video. Cooked for about 2.5 hours at 15PSI

[@jensraberg8620]

Thanks for wonderful videos!

[@shellytanner4727]

Is that "Bassnectar" playing in the background while loading 1st pressure cooker?

[@ShiestyDogs]

Bro I appreciate the knowledge but question, I’m doing this rn and I need the song please! I love this music lol

[@robbyhanlon]

Can you help me understand why growers don’t atmosphere sterilize grains?

[@Mossycreekmushroomsofficial]

You can't atmospherically sterilize anything. You have to use pressure to sterilize. The best you can do at atmospheric pressures is pasteurize, which isn't enough for media you plan to expand.

[@kennethgottfredsen1326]

Hello. Do you have a link to the website where they sell the pressure cooker? Thanks!

[@MycoTooMushed]

Love the music and content

[@DeannaPhillips-l9k]

Have you ever used pool chlorine to sterilize substrate?

[@jammin093]

love the music choice!

[@Elvampiro.]

Do you seal these bags after ??? Thanks

[@kylewhitlock8923]

Can you put substrate and grain together in the bag when sterilizing in pressure cooker?

[@klisaification]

Hello, can you tell me is it possibile to sterilize spawn in microwave? since i dont have pressure cooker..

[@joshcook9487]

You're awesome thank you for the video, cleared up a lot!

[@epica85smith]

How many sub bags do you inoculate with 1 5lb grain bag?

[@pyrrhospizza5938]

I consistently get 2 of 6 bags melted and burst, and I can't tell why it keeps happening. Does't happen in the regular PC since I added extra water. Any idea why this is happening in the autoclave? Thanks!

[@Shanesxperiments]

You are the best bro!

[@zen_of_I]

So no need to put rings between the bags and the inside of the pressure cooker?

[@pewpster]

Yeah I followed the video and my bags melted

[@Philipmgabler]

No links for the pcs??

[@mikehancho1613]

I was always told if your grain bag/jar is under water it is not steralized?

[@ariesmichael9993]

What bags are you using?

[@thanielxj11]

Love this channel

[@Bozo825]

Shouldnt the bags be already sealed when they’re placed into the pressure cooker?

[@spikespiegel2537]

Hopefully someone can answer this question I have.. do wr leave the filter patch facing out when we fold the bag down, or filter patch inside against the bag? Will doing it on the inside potentially cause the bag to burst if air needs to be released?

[@pumptydumpty7600]

most videos ive seen they fold it so its on the inside

[@lrw82tn]

I’m sterilizing some fruiting blocks for the first time ever as we speak….I would have assumed you wouldn’t want the filter patch touching the water. Am I off on that? I turned my filters up…..guess I’ll know if I messed up in the morning!

[@sunnyjones682]

What was your outcome? Filters in water or no? Filters folded inside or outside?

[@HerbQuest]

How much water did you put in though?

[@josephbrown7502]

You always have the tastiest music in the background, please include title and artist!

[@garrettjpeterson6440]

Thank you for this video! I’ve been using this method, packing 4x5lb bags (unicorn 3t) into a presto 23qt. I’m getting at least one popped bag per run, sometimes two. Do you have any advice? I’m venting, Keeping at 15 Slowing down cool down I don’t know what to do. :/

[@LarsLarsen77]

Sometimes the bags melt if they touch the side of the PC at the bottom. Usually it's from too much heat going up the sides of the PC. Maybe try heating it up slower with less heat on your stove, or put silicone baking mats between the bags and the sides of the PC.

[@Mike-cf9lw]

I just had two bags open on me. Would foil work on the sides of the bags?

[@dennisthamenacee]

What bags do you use?

[@pewpster]

I followed your Presto method to the T and had my bottom bags melt and open, and one of the top bags slightly melted. Am I having my burner too high? Did I run out of water?

[@pewpster]

Is that a thumbs up for melting bags?

[@brownsinglet4958]

It sounds like you run out of water. Did you see any water in the pressure cooker when you opened it?

[@timmyvolta1592]

do not exceed 124 Degress Celsius or your bags will melt, and of course keep enough water inside

[@jorgevelezterapiassonoras5138]

Why all american have vacum and the presto dont? And why still work the presto whitout the vacum?

[@slimgrows]

What hot plate u using buddy??

[@everythingomnia]

Do you sterilize the substrate that a jar of grain spawn goes into also? Or is this unnecessary as the spawn is already formed and there's plenty of mushroom to win the substrate?

[@aaronroberts3555]

I know this is off topic, but with a spent block what do you do with the bags? May I also get the exact dimensions for your fruiting bags for the 12 lbs of substrate.

[@eyesofthecervino3366]

Unfortunately I'm pretty sure mushroom bags are single use. I'm currently experimenting with using gallon jars instead for cultivating mushrooms, but it's already pretty clear it's a lot less convenient than just using the plastic.

[@jesseakc]

Thanks for the upload. I've been trying to contact you. I've joined your patrion but I don't know how to get the rewards.

[@psychomaniac189]

where do you get the bags, the grain, and the pressure coker?

[@J155P]

Internet

[@sabrinawn351]

Could i possibly use a instant pot???

[@valerievasiliou4013]

It won’t reach 15 PSI.

[@hannahfitts3453]

What size is the pressure cooker?

[@injegomontoya370]

Bruh you remind me of Stingray from Cobra Kai! Thank you for the information!

[@jollywarcry]

So these are substrate bags? How do you colonise these. Lc, grain or agar. I thought substrate was made up of sawdust etc etc. Thank you.

[@otacon1024]

A substrate is anything that will support mycelial growth. Also, doesn't matter what you inoculate with, it's all just mycelium (or spores that will soon become mycelium) growing on different substrates.

[@mtbraden1969]

Where do you get your bags?