How to Make No-Pour Agar Plates for Growing Mushrooms

[@JinTalzeus]

Nice, I first saw these containers being used by Mossy Creek. They provided a bulk warehouse link that is way too costly for me. Thanks for sharing this Amazon link. Will try!

[@peterbaryshnikov5411]

Ive been using this technique for a couple months. I ended up saving a good amount of money but it was a bit challenging for a novice at sterile technique. The drawbacks are condensation and difficulty working with one hand. For condensation: When my plates leave the cooker they are stored upside down so any residual water falls to the lid. When I use a plate I unscew it upside down and right it. Then I let the water drip off the lid before I screw it on. One handed: When I use traditional petri dishes its so much easier because one hand can hold a scalpel and one can manipulate the plate. Unscrewing these with one hand is possible but it takes a little dexterity. Keep in mind that these are smaller than petri dishes so I typically use the whole plate to innoculate grain. Buy more than you think you’ll need and buy your agar mix in bulk to actually save money.

[@treybegood]

Any other tricks to prevent condensation? Maybe wrap the dishes in foil prior to pressure cooking???

[@81Sazerac]

Another Banger! Love the different methods you showcase, especially those that help save money while streamlining workflow. 💪

[@SporenSprout]

@@81Sazerac Thanks for watching!

[@LarsLarsen77]

I love these little things. They're great for starting seeds in tissue culture too.

[@SporenSprout]

@@LarsLarsen77 I need to try that

[@ZombieSnax42069]

Lolz amazon needs to give you a discount, since you shared those twist tops everyone bought them all up. Its been 3 weeks and they stay sold out .😂.

[@SporenSprout]

Dang that’s crazy 😂

[@therabbithole223]

I love this, its a great way to cut down on the waste we produce. What are your thoughts on how thick that your agar is? Is that just using what you make or do you actually like it that thick? I usually pour my plates about 2mm and ketchup cups are thicker which I like for keeping samples longer. Keep up the great work.

[@SporenSprout]

Yeah I was just trying to use most of what was made but I do like thicker agar so it lasts longer and doesn’t dry up.

[@WhoisMichelleCollie]

Its been amost a year, are you still using these or did you go back to the reg petri dishes. Because I see your new videos with petri dishes.

[@jayrlbd8355]

Omg, I'm obsessed with your mushroomy heat mat!!

[@ashboom2175]

thanks for this video! typically use ketchup cups since they're easily accessible for me and less hassle than the petridishes but these look fantastic. I'll definitely be giving them a try, are they able to be sanitized and reused?

[@SporenSprout]

Yes they are awesome! They can be cleaned and reused.

[@dantefuego]

Hey bro once the cooking cycle is finished, do you open the PC top at all during the cooling process? and How long can you expect the cooling process to take on average?

[@kevinsmith9190]

I’m going to try this.

[@ChuckNoctis]

The poured small ketchup cups, do you store those with the lid on and in room temperature?

[@MissHelium]

Great idea I will try it 🙌 Thank you for the video

[@SporenSprout]

Thanks for watching! 🍄

[@SkornClawYT]

(1st Question) How long can you wait between pouring these and pressure cooking them (2nd Question)How long can you store them after pressure cooking before you use them in a SAB or in front of a Flow Hood. And would you recommend learning to do Agar or Liquid Culture Method first? (I know people often use Agar to LC, or Agar to Grain). I'm Currently Working on growing Yellow Oyster Mushrooms with your instructions!

[@chrismehl9842]

Learning agar and liquid culture side by side is extremely helpful. You can innoculate an LC jar with agar or an LC syringe and then after colonization use agar to test your LC jar for contamination. Once your contamination free you can again use agar to innoculate grain bags, jars, or even more LC. It's a beautiful cycle really.

[@jeremyjetson1965]

Will they last 45+ mins at 15psi, say if I wanted to sterilize some grains at the same time?

[@SporenSprout]

Yes definitely

[@jeremyjetson1965]

@@SporenSprout awesome they are in there now, thanks for bringing this tek to my attention.

[@davidsanchez-rs1np]

Hey men ! I already tried with these plates in a presión cooker, however, when i opened it after cooled down all the plates were deformed from the pressure and the agar was still liquid. Any idea why?

[@SporenSprout]

Sounds like you didn’t leave the lids loose while pressure cooking

[@kevinsmith9190]

I’m a newbie and my agar is white except for a few dots of dark brown spots close to the edge and the white is in middle where I squirt the LC. ?

[@BriggsWare]

Hi, Might be a stupid question but what are the main reasons for using plates, liquid mycelium, and grain? How do you use them, and why? Many thanks

[@SporenSprout]

Watch this video: kzbin.info/www/bejne/e3K1f62EfaatnJI It will answer all of your questions.

[@ebbstubba5845]

This seems great, but I just can't get them to work out. Almost every one has water sloshing around on top of the agar, even if I let the plates cool considerably before pressure cooking. Any advice?

[@SporenSprout]

After pressure cooking and solidifying, place them in a hot room. That will solve the problem, agar will absorb the moisture over a few days in hot room

[@ebbstubba5845]

@@SporenSprout I'll try that out and see how it goes! Thanks!

[@hardstylestrength]

Could you do this with glass petries and parafilm? Just load them up before sterilization?

[@dantefuego]

Thats a good question

[@danielchen9841]

How u iniculate the container

[@SporenSprout]

Just twist off the top and put a drop from a syringe in the middle and put the top back on.

[@rickheath5507]

I put an injection port on mine and a filter just like I do on my grain jars.

[@Kyle3x]

Could I use these cups and make modified lids for them?

[@SporenSprout]

@@Kyle3x Yes

[@joychukwuemeka2622]

How can one get the Agar powder, and Agar plate

[@SporenSprout]

Agar powder: amzn.to/48jz9qi Sterile Petri dishes: amzn.to/3YzSK2p

[@sara_7595]

What temp do you pour? I have tons of condensation

[@SporenSprout]

It’s around 120F right before it gets goopy. Some condensation is normal. Store the plates in a warm room around 78F for a few days after pouring and the condensation will go away. You can also use a heating pad and set it on top of the plates to clear the condensation.

[@sara_7595]

@ awesome thank you 😊

[@Shahbeyke]

Is it enough to use only agar agar???

[@SporenSprout]

Agar agar and water will make “water agar” which is good for cleaning contaminated cultures but produce weak mycelium with no nutrients. The mycelium still will grow but faintly

[@MustHavesbyDawnlarae]

Great videos and products. 🍄‍🟫

[@SporenSprout]

Thank you! 🙏 🍄

[@angelomespinoza]

like your agar content! Are you from SagNasty? i grew up there

[@SporenSprout]

Thanks for watching! Yes I am 😂 that’s crazy!

[@dominicporta6127]

@@SporenSproutsheesh I’m from around the way too!! Ann Arbor

[@SporenSprout]

@@dominicporta6127 Nice! I like going to the decriminalize nature event there!

[@dominicporta6127]

@@SporenSproutI’m gonnna go to the event next year! Did you not use food coloring with these plates?

[@ahosie]

Wish I could get containers like this in Australia, but for some reason this seems to be a US only product for now

[@mlowes23]

ScienceEquip online store sells polypropylene petri dishes, it's just they are not twist top. I have a bunch at home, so will test it out and see at some point using the above method, except sealing once sterilised. Also might need a weight on top so there is no boil over in the pressure cooker.

[@ahosie]

@@mlowes23 the twist top is the major selling point for me - I'd be happy to never have to cut and wrap parafilm

[@mlowes23]

@@ahosie I use glad wrap, but agree and would also eliminate more plastic waste with the twist top

[@__shaun__]

on ebay there are aussie made ones (can see Product of Australia in the photo) but i'm waiting shipping so grain of salt "250ml 500ml Plastic Clear PP Round Jar 90mm Diameter Screw Lid Food Storage Box" different dimensions but look usable and are PP dishwasher/microwave stuff, seems legit

[@AurumAura31103]

Try Temu!! They have all sorts of containers.

[@daverojas7351]

Don't they loss their shape a little bit when sterilizing because of the temperature and pressure? I know it's PP, but I use another brand of PP plates for agar, and they get deformed a little bit each time I sterilize them.

[@SporenSprout]

No these containers don’t warp or become deformed after pressure cooking. I have re used them multiple times and haven’t noticed any difference.

[@shrivanth6774]

Thats great

[@SporenSprout]

Thanks for watching! 🍄

[@devilsadvocate1338]

How do you not end up with lots of water in the agar cups

[@SporenSprout]

@@devilsadvocate1338 I just keep them in a warm room for storage and the agar reabsorbs moisture

[@devilsadvocate1338]

@@SporenSprout does leaving them in the pc to cool help?

[@renzcorpuz1989]

@@SporenSproutdo you just leave them on an open air in a room? What should be the temp? And how many days before it reabsorb moisture? Please reply

[@SporenSprout]

@@renzcorpuz1989 keep the agar cups closed in a room that is 75-78 F for 1 week to reabsorb. You can also try flipping them upside down. If none of that works try using less water in the recipe

[@renzcorpuz1989]

@@SporenSprout got it thats what im doing right now lets see if it reabsorb if not. Im thinkin of shaking them after PC while theyare hot to get the moisture on top. Thanks for the reply!

[@Mr.Gandy.]

danke.

[@pressman45]

Where did you purchase your twist top dishes?

[@SporenSprout]

@@pressman45 amzn.to/4eUbPSY

[@pressman45]

@@SporenSprout thank you

[@pressman45]

@@SporenSprout Your videos are amazing. Thank you very much

[@SporenSprout]

@@pressman45 I appreciate that! Thanks for watching!

[@WallStreet30]

💯

[@goatrock123]

Are these pp5 and will they withstand 2 hours of pressure cooking?

[@SporenSprout]

@@goatrock123 Yes they are

[@troyin17331]

Ok so I'm confused. It's "no pour" but you are pouring it into the dishes. So how is it "no pour"?

[@GrowYourOwnFun]

No pour refers to not pouring post sterilization. He pours pre sterilization thus reducing the chance for contamination

[@WhiteBeardHashtag1205]

@@GrowYourOwnFun still doesnt make sense to call them "no pours"

[@orionsbelt3827]

How else would he get them in to the plates? Lol

[@MissBlackMetal]

I earnestly agree, the term should be changed as it makes zero sense. The method should be called "pre-PC pour".

[@WhiteBeardHashtag1205]

@@MissBlackMetal I think pre-pour would suffice, but I agree completely with you

[@Since-80s]

hey i tried the discord link, it links to your discord but it says unable to accept invite

[@SporenSprout]

Thanks for letting me know, here is an invite link: discord.gg/atfa4BZpBM I’m not sure why that happens sometimes.

[@joychukwuemeka2622]

How can one get the Agar powder, and Agar plate

[@SporenSprout]

Agar powder: amzn.to/48jz9qi Sterile Petri dishes: amzn.to/3YzSK2p