NEW LIONS MANE BREEDING TECHNIQUE - collecting spore isolates real time! (no microscope necessary)

[@JustiNourse]

I use this method for getting spores from mushroom caps too... I got a tall container that I suspend a bread tie from the top with a magnet... Stick the bread tie through the cap and suspend it over the petri dish.... I get a lot of single spores to isolate for my monokaryon breeding purposes.

[@jidun9478]

That's cool 😊 thanks for sharing again! I just started my mushroom cultivating adventure last November. I got a lions mane liquid culture from you at the same time I ordered two of the 7lb lions mane fruiting block kits from MycTyson to make sure I could grow them🙂 They were fabulous to me, I got about two pounds from them on their first flush and just finished them off this weekend with the fourth flush about 1/3 of a pound just in time to put out my 8.5 pound block that's in my fruiting chamber from your strain yesterday, It's popcorned on the top and mycelium is growing everywhere else. I took a clone of the MycTyson lions mane to agar and it's ready to put to liquid culture now. I just want to say thanks, it's guys like you that I have learned soooo much, and I have done it all in a large still air box.

[@FreshfromtheFarmFungi]

Awesome thanks for watching! It sounds like you have got your work cut out but those two strains are strong! MUSHLOVE

[@Analyticpessimist]

The simplicity of well thought out work. Very cool video. Thanks.

[@kemaltasyurek]

you are an inspiring scientist, thank you Gary.

[@emeteriogomez3748]

Super great info and tek. Youre one of the best out here

[@FrankEmmanuel799]

I’d appreciate your comment

[@FrankEmmanuel799]

I got some lion’s mane shrooms from an online supplier

[@FrankEmmanuel799]

FROM CRUISE_MYCOLOGIST.

[@urwhatueat2170]

Nice technique, thanks for sharing

[@soccer2themax]

Excellent idea. Follows nature. Thank you so much.

[@cthomas254]

where's the other 10k views this video deserves?!

[@JeanBrnl]

Great technique

[@Snoeijhard]

My thoughts .. if you speed up the proces, are you not taking away the chance to grow the power?

[@1ntwndrboy198]

If I have a jar with spores from dried out mushrooms can I use those spores?

[@pamelawillis9714]

Super interesting. I’ll be watching to find out what happens next. Thanks!

[@youssefrochdi1994]

Thank you for the valuable information.

[@bounceurabdelaziz4973]

Thank you very much for your excellent video.

[@ErikAnders]

I was wondering Abt this in my head. Very cool.

[@Alexmw777]

interesting technique, i would have thought the flow hood would make collecting the spores difficult

[@FreshfromtheFarmFungi]

in normal circumstances yes but you can turn it off or protect from direct air flow

[@willit007]

pretty nice idea

[@absurdsanjuanino]

What about for home mycologists who don't use agar? Could you do that set-up, maybe with a smaller grill and a tupperware container inside a SAB--and have it fall onto aluminum foil? Would you then be able to make a spore syringe? You wouldn't get the different cultures to compare but maybe you could avoid contaminants.

[@FreshfromtheFarmFungi]

yes that could work as well 👍

[@oldguy3525]

I want more like this... thanks

[@Zayskibop]

Any question I have, I end up on one of your videos now😂😂 Thank you for all the great content Gary ❤️

[@ScreamSheLovesMeNot]

No sectoring? When your making those LM transfers wouldn't they contain multiple spores? So it would be far from an isolate.

[@FreshfromtheFarmFungi]

Please explain - It is my understanding as the spores are dispersed, they will germinate and the sectoring only takes place after the hyphae interact with another spore isolate. These germinated colonies of hyphae are isolated onto a petri dish before they have a chance to pair - thus creating isolates. Sectoring will happen in the next process

[@ScreamSheLovesMeNot]

@@FreshfromtheFarmFungi I understand. I guess that wouldn't be necessary if you were able to limit the amount of spores gathered on the plate prior, like if say 1-5 all fell onto their own plate? And even then there's no way 1-5 would only contain just 1 spore, so they would inherently mate immediately after germination?

[@FreshfromtheFarmFungi]

yes there is this error, but the spores that hit the outside have a greater probability of being single spores - only the next step will determine if they are compatible or not - possibly letting them go on the first plate and taking sectors after they grow out would be just as easy or easier - Thanks for the idea! In the end I just want a better lions mane culture

[@ScreamSheLovesMeNot]

@@FreshfromtheFarmFungi same! Your so helpful man. Thank you! ❤️

[@mushtemporal8295]

@@ScreamSheLovesMeNot I had the same thought. There's no way there is only one spore germinating in each of those hyphae islands.

[@alejandrobatalles]

hola !! soy de argentina , estoy cultivando cubensis !! he visto tu video y que me ingredientes me recomendas para hacer mis placas ? y que queden asi de cristal? saludos

[@CMZneu]

1:05 It takes 2 weeks inside the fitting room for it to fruit? Is that normal, how long do oysters normally take because i'm having some trouble with mine, they take like 3 weeks and i'm not sure if it's because the conditions are not optimal or it's normal.

[@mycroken8582]

Very cool idea for making a multi spore inoculation to agar. Interested to see results on this technique.

[@roadwanderer1366]

Awesome idea bro

[@reedanthony106]

👀 Haven't seen any finished product lately, just mycelium on petri dishes. Do you have a mature shroom to show us?

[@FreshfromtheFarmFungi]

check out our instagram - this channel is heavily focused on my lab procedures and sterile work but I will try to add more fruiting bodies

[@reedanthony106]

@@FreshfromtheFarmFungi😎✔ Cool beans, thanks.

[@codelicious6590]

Awesome

[@FunkyGreen]

About how long do you keep each dish under the fruit to make sure to not get too many spores

[@FreshfromtheFarmFungi]

I was pulling out new plates every 15 minutes or so

[@guccipitts]

Wonderful 👏

[@Algumnomequalquer]

You can't clone them by tissue?

[@FreshfromtheFarmFungi]

you can - but this was for breeding purposes

[@Allyourbase1990]

My lions mane on agar doesn’t look like my turkey tail or other species . It’s such a thin little layer on agar and doesn’t look like other peoples . Why does it do that ?? It’s normal ?

[@FreshfromtheFarmFungi]

yes it’s normally thin and wispy until it fruits out

[@islaminheart3834]

How can I separate spores from mycelium! Please let me know

[@FlakeyPM]

You are not worried that most of the spores will be incompatible with each other i.e. sister spores and therefore not be able to fuse? I would be looking at using spores from 2 different lions main strains

[@FreshfromtheFarmFungi]

maybe but it’s a lot simpler - going for the “quick and dirty” approach- shortcuts usually are not as precise

[@mushtemporal8295]

That's not usually a concern, is it? The sheer amount of genetic variation available when you're talking about millions of haploid spores seems like it would take care of any incompatibility among spores.

[@HeyHeyHarmonicaLuke]

Are you related to Greg Proops from Whose Line Is It Anyway? :D

[@Cams_Stuff]

Do you have to do anything in particular to trigger the fruit body to drop spores? Or does it just drop them by default after harvesting? I'm a total noob but am soaking up so much info from your channel. Thanks so much!

[@FreshfromtheFarmFungi]

they drop their spores based on maturity- you want to pick them just before they drop them

[@urbanvegsa1928]

What temperature should I be pouring agar? I poured at 160 degrees the other day and had condensation. The next day I had contamination. Should I be avoiding condensation and would that have caused the contamination?

[@FreshfromtheFarmFungi]

I pour mine around 160 all the time - I leave the plates in front of the flow hood for a day or 2 after to allow the condensation to evaporate. The contamination could have come from anywhere - this is where troubleshooting begins. Most likely it is an error on the technician. If you sterilize agar in a jar, leave it closed and it contams, then there is a problem with your procedure, if not, there is a problem with your pouring.

[@وهابالربيعي]

big thumb up ,,, thx a lot

[@tarheelgarden]

did you get a faster Lions mane yet? the one I use is 10-12 inc and 9-10 day fruiting.

[@FreshfromtheFarmFungi]

I have 3 different strains - I like the one I have been growing because of the density but that is probably why it takes a little longer

[@tarheelgarden]

@@FreshfromtheFarmFungi cool. I'm trialing 3 new strains and a comb tooth. We can trade if you want.

[@vonries]

I wish you the very best of luck. Are you going to be marketing the best stain once you are done with testing? I hope so! I was victim of 3 auto accidents, each with head trauma. I don't know if it can help me like it does alzheimers patients, but I plan on trying to find out.

[@FreshfromtheFarmFungi]

We release new strains regularly on ETSY most definitely if this one is a big yielder it will go up!

[@imurgodsgod]

You talking above the open agar plates is making me hear flash backs to my-lab partner yell at me

[@herbalvisionz6091]

How can you be sure that the spores are isolated?

[@FreshfromtheFarmFungi]

with DNA testing - this is not a perfect process but a lot better than the previous method I was using for lions mane - another approach could be to let them breed on the plate, wait until there are clearly defined phenotypes and select them out when they have isolated on the original plate - the issue with this is that you would never obtain the haploid progenator. Moving forward there could be mated pairs that I consider “isolates” but that will reveal itself in the next step when it doesn’t mate with the other pairing. Hope that makes sense - shortcuts are never perfect

[@herbalvisionz6091]

@@FreshfromtheFarmFungi cool thank you for the explaination! I look forward to updates! I am going to try it out!

[@devinfenwick1134]

What flow hood system are you using?

[@FreshfromtheFarmFungi]

air science for culture work and myers mushrooms for my inoculations kzbin.info/www/bejne/rGbPZqyEeN2meNU kzbin.info/www/bejne/r4XTl2Z7gtdkiac

[@devinfenwick1134]

@@FreshfromtheFarmFungi thanks!

[@macmckulis7440]

Awesome dude. I think the second flush should fruiting should provide better genetics, it is just my theory, make use to keep track witch bag fruits the quickest and then compere first fruting with the second flush. So the theory is that genetics are more established in the 2nd fruting compere to first, there fore creating stronger genetic code, what do you think brother?

[@FreshfromtheFarmFungi]

It might be possible - I think epigenetics can play a role in this if the mushroom is more developed later on - the only way to verify would be genetic tests

[@macmckulis7440]

@@FreshfromtheFarmFungi yes ofcourse testing genetics would be the best. But would it not be pabible to see the results of it by growth in a dish, plus eventually in substrate?

[@johnandjacquelinewarren9979]

@@FreshfromtheFarmFungi I don't understand why this would be true, but it could explain why that blob mushroom came out normal on the second fruiting in the recent "immaculate inoculation" experiment you did, right?

[@coronnation8854]

This is cool and interesting. But damn I just wanted to grow some lions mane for the first time, and sustainably. I want to do it properly, but I already have way too many complicated hobbies. And this seems like it would be a bit much, unless I just buy a premade bag.

[@LesDL69]

Hey bro! Love your content. Currently made my own agar but I got some questions. You got an email?

[@FreshfromtheFarmFungi]

[e-mail]

[@LesDL69]

@@FreshfromtheFarmFungi tank you friend. Mush love ❤️

[@LarsLarsen77]

You shouldn't set petri lids face down like that, always face up. The steel surface is merely disinfected, whereas filtered air is sterile.

[@kartalbaba981]

👍👍👍

[@LarsLarsen77]

You can't see that it's a single spore isolate, you need a microscope. There could easily be two spores there and you can't tell with the naked eye. It's LIKELY it's a single spore, but you don't know.