Fun with Mycelium: making a liquid culture from a colonized BRF jar

in this video I demonstrate an experiment I did with a colonized BRF jar of Pes Amazonian. I made a karo LC with a sterile syringe.

[@LazerCatHelmet]

I expand cultures when I get them (Peptone and malt). However, my syringes never go empty anymore either. When it gets down to a few MLs, I simply suck up some fresh lc mix. Syringes will “colonize” much faster than jars of LC. Sometimes I do something very similar to this method above. TIP- you can get extra long blunt tip needles and sharpen them on a fine stone. This is very handy for reaching past the verm in pf jars, pulling LC without gettting liquid near the lid, and for getting all over the place when NOCing up jars or bags. I have a syringe thats over a year old, never been in a fridge. It all started when I got a culture syringe that had a visible chunk (looks like they used blender) that would never come out. If you dont let them sit forever, and use proper sterile methods, it seems like you can keep it going quite a while just giving it more mix.

[@mycopsycho3130]

Right on🫠🍄👍 gotta love LC

[@rosariocatlin4845]

Could you just use sterilized water from a vacuum sealed container of sterilized water ? I work in the medical field so just curious

[@mycopsycho3130]

Sounds legit to me

[@RadioRedLight]

So you squirt it in the colonized jar and then extract it back up? Or you just hope you get enough on your needle ?

[@mycopsycho3130]

Squirt it and suck it back up

[@MIR-INDA]

наполните ванну 1 мл кипятка, затем стерилизуйте под давлением в течение 15 минут при закрытой пробке. Игла для стерилизации пламенем. ввести иглу в банку для колонизации. игла соберет маленькие кусочки мицелия. брызните на иглу спиртом и наденьте чашку. протрите инъекционное отверстие медицинским спиртом вставьте иглу и нажмите на плангер вода вытолкнет кусочки мицелия, будут кусочки вермикулита - это нормально

[@mycopsycho3130]

👍This is exactly how I did it 😁🥳🍄