very easy and informative video, you have explained it very well, thank you ma'am, but no explanation about the addition of ladder and positive control and negative control, plz tell about these too ?
@ScienceCreAchins6 жыл бұрын
A DNA ladder is just a standard set of DNA fragments that are various, but known, lengths (bought from a biological supply company). They will be pulled through the gel, with the smallest pulled through faster than the larger fragments. When the gel is disconnected and stained, the ladder can be compared to the unknown fragments in other wells/columns, so their lengths can be estimated. (The ladder is used to make a standard curve with semi-log paper (distance vs. length of fragment), and the unknown fragments can be estimated by using the curve made with the semi-log paper). I don’t think we actually used a ladder or controls for this lab, just the three strains of (fake) virus DNA (it's a 9th grade, intro to biotechnology lab). Here is a link to the lab directions: www.cpet.ufl.edu/wp-content/uploads/2013/10/Outbreak_virus_dna_teacher_manual.pdf
@scolary51476 жыл бұрын
thank you for your video and further explanation
@GodiyaMoses5 жыл бұрын
this is a really informative and detailed video thank you so much
@swapnadeepd73632 жыл бұрын
Wonderful video.....I am from the field of Biotechnology and also in the teaching profession....I loved viewing the video😊
@swapnadeepd73632 жыл бұрын
Beautifully made video 😊 I myself am from the field of Biotechnology and also in the teaching profession...loved viewing the video 😊
@lamie7767 Жыл бұрын
hey! i am an aspiring biotech major and had some question that i want to ask if you have time!
@MrSoumyaBanerjee4 жыл бұрын
Thank you, this was very illuminating.
@nibrassbio89373 ай бұрын
Thank you for this wonderful video ❤❤❤I like it so much ❤❤❤❤
@AuraNaturae3 жыл бұрын
Ethidium bromide is orange dye . But here it is voilet which dye it is.
@082.apoorvanidhiacharya85 жыл бұрын
What are used as DNA samples???
@ScienceCreAchins4 жыл бұрын
We used DNA from this kit: www.carolina.com/dna-gel-electrophoresis-kits/outbreak-fingerprinting-virus-dna-8-station-kit-dna-only/211204.pr
@ramkeshsaini93456 жыл бұрын
Tell we CDNA library
@diksha87563 жыл бұрын
Awesome
@ramkeshsaini93456 жыл бұрын
How to attach DNA in vacter
@vineetpandey53092 жыл бұрын
Probably nice video.
@BarbacoaAppliances8185 жыл бұрын
Those googles though, lol I bet her eyes were boiling from steam and pressure lol