What is Gel Electrophoresis | Don't Memorise

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📅🆓NEET Rank & College Predictor 2024: infinitylearn.com/neet-rank-p... How do we separate the desired DNA fragment from a complete mixture of several DNA fragments? The job sounds almost impossible! But there is a technique that comes to our rescue! Gel Electrophoresis is a separation technique which separates DNA fragments based on their Length or Size. Want to know how the technique works? Watch this video to understand the Principle and Technique of Gel Electrophoresis.
In this video, we will learn:
0:00 Introduction
0:44 definition of Gel Electrophoresis
1:19 is DNA charged?
2:20 principle and working of Gel Electrophoresis
2:48 principle of separation of the DNA fragments
3:57 requirements for Gel Electrophoresis
3:55 Gel Electrophoresis process
6:24 DNA separation
7:25 Elution
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[@franceraymundo3786]

Raymundo, Francezka J. •Gel Electrophoresis is a separation technique which separates DNA fragments based on their Length or Size. •The movement of charged molecules is exposed in electrical field and it occurs in gel medium, that's why it's called Gel electrophoresis. •To observe the DNA molecule, agarose gel is used with ethidium bromide (ethidium bromide is used beacuse when the agarose gel containing the DNA is observed under ultraviolet light, bright color bands are clearly seen). •Gel electrophoresis is considered to be one of the important techniques in recombinant DNA tech.

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[@colleensalting1138]

Salting, Colleen L. 8-Avogadro -The separation of DNA fragments based on their sizes is called Gel Electrophoresis -Technique is based on the movement of charged molecules occurring in an electrical field; occurs in the Gel Medium -The DNA is negatively charged due to Phosphate groups -The DNA fragments may contain DNA fragments. Because of different sizes those which are smaller move ahead, and those larger in size move slower -The gel has a mesh-like structure, similar to a sieve Gel Electrophoresis: -Since the DNA is negatively charged it will move towards the positively charged electrode -Using a colored loading dye can help identify and track the DNA in the gel since they are invisible DNA separation: -Ethidium Bromide easily binds to the DNA molecules; this helps us see bright-colored bands under UV light -To know the size of the DNA bands a DNA Ladder is used -Elution is the manual extraction of a DNA fragment from the Agarose Gel used -Gel Electrophoresis is considered one of the most important techniques in recombinant DNA technology

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[@riiel21]

Tiongco, Gabriel Felix D. 8-Edison Gel Electrophoresis • It is considered as one of the most important techniques in rDNA Technology. • It is a separation technique which separates DNA fragments based on their length or size. • It is based on the movement of charged molecules when exposed to an electrical field (occurs in a gel medium). • DNA is negatively charged. It is due to phosphate groups which are present in the backbone of the DNA's double helix. • The gel generally used for DNA is agarose gel which are obtained from seaweeds. • Treating ethidium bromide to the agarose gel makes it possible to see the separated DNA molecules because ethidium bromide easily binds to the DNA molecules. • The DNA ladder is a standard chart used to compare the bands obtained to easily identify the desired DNA. After obtaining the desired DNA, we can cut and extract the DNA fragment from the agarose gel which is a process called elution.

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Thank you so much🙏 Best explanation till date I got for one of the most important molecular biology techniques -Gel electrophoresis.

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[@brendanliamrebote699]

Rebote, Brendan Liam P. 8-Avogadro Gel Electrophoresis • used to separate the different DNA fragments based on their sizes • movements of charged molecules when exposed in an electrical field and this movement occurs in an gel medium, hence gel electrophoresis. Process • DNA negatively charged (Due to phosphate groups) • Phosphate groups are present in the backbone of the double helix. • Agarose gel is usually used for DNA • The charge across the gel helps the mobility of the DNA fragments. • Not all fragments move at the same pace. (smaller fragments move ahead, bigger fragments find it difficult to move) Procedure • Requirements for gel electrophoresis - DNA sample - Gel - Casting tray - Comb - Electrical Supply • The wells are formed from the negative charge so that the DNA will go from negative charge to positive charge. • A colour loaded dye is used to track the movement of the DNA samples. •Buffer is used for better conductivity of electricity. • The loaded dye is much faster than the DNA samples because it must reach the anode first. If the loaded dye reaches the anode, it must mean that the DNA samples reached somewhere near. • To observe the DNA samples, we treat the agarose gel with ethidium bromide solution. • Ethitidum bromide is used because it easily bonds with the DNA molecules • DNA bonds can be seen under a UV light • DNA ladder is used to compare the size • Cutting and extraction of DNA fragment from the agarose gel is called Elution. •

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[@AngelicaMayDSalvatierra]

Angelica May D. Salvatierra : Bohr - Gel electrophoresis is a technique for separating DNA fragments depending on various sizes. - It is called "Gel Electrophoresis" because it is based on a movement of charged molecules in an electrical field that occurs in a gel medium. -The process of Gel Electrophoresis needs tools such as a casting tray, gel, comb, electrical supply, and DNA Sample (Different sized DNA Fragments). -The DNA molecules are seen using an agarose gel with ethidium bromide (quickly attaches to DNA molecules). Light brilliant color bands can be seen when the agarose gel containing the DNA is examined under ultraviolet light. - The size or length of a DNA fragment can be determined by comparing the bands obtained to the DNA ladders (standard chart).

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[@maryjoycezalamea1722]

Zalamea, Mary Joyce H. 8 - Edison Gel Electrophoresis - Gel electrophoresis is a method used for separating DNA fragments based on their sizes using Agarose gel, a gel that can be obtained from seaweeds. - A DNA molecule is negatively charged due to the phosphate groups found in its double-helix structure. - A colored loading dye is added to the mixture to easily track the DNA due to it being colorless. - DNA samples are loaded near the negative electrode side of the gel as they move towards the positive electrode using the electric current. - Agarose gel is treated with Ethidium Bromide solution, as Ethidium Bromide can easily bind to the DNA molecules. - Elution is the process of cutting and extracting DNA fragments from Agarose Gel.

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[@akichu_08]

Fernandez, Akiko B. | 8-Chatelet - Gel electrophoresis is the separation of DNA fragments on a piece of gel. It is based on the movement of charged molecules when expose to the electric field. It was said that Gel electrophoresis is considered a very important technique used in recombinant DNA. - The charge present on the DNA molecule is negatively charged due to phosphate groups. - Agarose gel is the gel used for Gel electrophoresis and it is obtained from seaweeds. - It is said that the smaller fragments move faster than the large fragments, and they move from negative to positive (they can track the DNA moving by using the colored Dye). - To easily bind the DNA molecule, treat the agarose gel with ethidium bromide solution. - DNA ladders will help us know the size of DNA fragments. - Elution is the cutting and extraction of DNA fragments from Agarose gel. - The extraction of DNA can be used for further downstream processing.

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