Gene mapping part 4 Somatic cell hybridization or somatic fusion

This gene mapping lecture explains about the somatic cell hybridization mapping. shomusbiology.w...
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Somatic fusion, also called protoplast fusion, is a type of genetic modification in plants by which two distinct species of plants are fused together to form a new hybrid plant with the characteristics of both, a somatic hybrid. Hybrids have been produced either between the different varieties of the same species (e.g. between non-flowering potato plants and flowering potato plants) or between two different species (e.g. between wheat triticum and rye secale to produce Triticale).
Uses of somatic fusion include making potato plants resistant to potato leaf roll disease.[1] Through somatic fusion, the crop potato plant Solanum tuberosum -- the yield of which is severely reduced by a viral disease transmitted on by the aphid vector -- is fused with the wild, non-tuber-bearing potato Solanum brevidens, which is resistant to the disease. The resulting hybrid has the chromosomes of both plants and is thus similar to polyploid plants. Somatic Hybridization was firstly introduced by Carlson[who?] in Nicotiana 'glauea' Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia.
Link- en.wikipedia.or...

[@user-ql6kr4gd2j]

You are my teacher of the year! I am writting a scientific work about g. mapping, i listen to your lectures and read some literature and surt the internet and I CANT STOP. this is so so intresting and exciting!!

[@shomusbiologyofficial]

All the very best. carry on.

[@user-ql6kr4gd2j]

Suman Bhattacharjee thank you! good luck to you too

[@sayednazimshah4432]

Sir what is cell line plz explain it

[@kashafrasul3952]

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[@shomusbiologyofficial]

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[@vishnuvish1647]

@kasaf rasul soo true

[@shafiaambreen7244]

This is the best explanation till date. Thank you Sir.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@waseemsheikh6676]

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[@priyankavats2241]

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[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@meghanagvi6106]

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[@shomusbiologyofficial]

You're welcome

[@sush9901]

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[@shomusbiologyofficial]

You're welcome

[@rajlaxmiprasad7087]

Easy to understand. U are a gift of god

[@shomusbiologyofficial]

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[@akashsingh-od3sy]

i was wondering for this concept but you cleared my all douts 🙂you are awesome sir

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Geostorm101]

Sir , this video is really very nice and conceptual. I understand each and every little concept or reason behind each and every step. 👍👍👍

[@sweta8135]

Happy teacher's day sir...

[@boomikamani2118]

Thank u for being life saver

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@fazeelatsheikh3539]

really informative.,.and easy to understand .,thank u

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@fazeelatsheikh3539]

yeah recently i subscribe your channel.,.one thing is very bad for me that i have lost much of my time .,.and now in exam i found u r channel

[@roseb2105]

also with this work also in diffrentiating between genes of the same tissue type

[@krishnatwayana1453]

isn't it possible that random elimination will effect in complementation ....and makes failure of hybridoma cells(which then no longer be) survival? ....Also whts the real need of hybridoma in this process of mapping?

[@arpitaghosal3354]

Sir this video is really helpful. Could you please tell me which book to follow and which chapter should I read for this topic?

[@rajashrimazumdar8390]

Best explanation ❤️❤️

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@themarilyncle]

Thank you! This really helped for my genetics exam :)

[@shikhagill555]

Sir if we want to obtain Human cell then Why we need to fuse Human cell with mouse. We can talk direct human cell

[@swarnalisarkar3739]

I have the same question right now... Why do we need to have Hybridomas we can consider human cell chromosomes only.

[@manishalohan4005]

To make random elemention of human chromosomes to make different cell lines 🤔🤔

[@a.h.m.safayetullahprodhan1797]

I have the same question too.

[@govtRamaniNirajBindeshwar]

We need rodent (mouse ) cell for the cloning process due to this we need to fuse them

[@aparnasai5326]

Hybridoma cell lines are more sustainable than human cell lines and uphold the expression of monoclonal ABs. Go through the reference: 10.1186/s43141-021-00264-6

[@chinmayeepanda2367]

Thankyou sooo much sir....

[@shomusbiologyofficial]

You're welcome

[@reebathakur5871]

Thnku so much for clearing this topic

[@ibrahimaldoori305]

it's really helping , thank you so much

[@pradeepsughra152]

Nice video i understood it

[@shomusbiologyofficial]

You're welcome

[@radhekrishnamotivationgang6115]

thank you sir so much

[@shomusbiologyofficial]

You're welcome

[@user-qg6bs7zn4e]

What kind of two cells can be fused together? Can we fuse every type of different cells together or is there any t&c for two cells to be fused?? Thank you for great effort in that age 🙂 Always a great inspiration for me Thank you.

[@linafievet7296]

Very clear and helpful, thank you!!

[@dashrthbhaliya9716]

Sir if we cultivate the cell having only human chromosomes than what is importance of fusion..We can simple replicate it via normal subculturing .???

[@ZimbardoBandura]

still not getting why would we need a hybridoma in the first place...can't you just take a human cell, take out the genome (which has all the human chromosomes), amplify the chromosomes/genes, run gel, and do all the relative placement thing? and if in the hybridoma case you use 1000s of cell lines, can't you just take thousands of human cells and run the gel? what's the point of making hybridomas anyway? thanks

[@wyattx008]

To make miniature human rat yeti's. Duhh!

[@AvnishRaghuvanshi]

@Raissa Regina Ng that mean before electrophoresis we must know a marker specific to a chromosome otherwise only microscopical examination was enough? Am I right?

[@souvikdutta2964]

To decrease the chromosome number we need hybridoma

[@shekharkumarsinoriya3700]

Why we form hybridoma.. we need to know about relationship between 2 gene by gene mapping..Why we can't run the human chromosome on electrophoresis directly to know about gene mapping..What is the requirement of formation of hybridoma here?

[@khushbooshaikh9919]

Awesome video sir... it helped me a lot.... :)

[@ibrarshahswaty745]

So what is the reason for making hybridoma? If we use only human DNA for mapping

[@nourelhodaharidy2820]

It is interesting! Thank you.

[@shikhagill555]

Sir Why somatic cell hybridization is required

[@sachinkumar-lz5bt]

very intresting

[@priyalahorani1706]

If finally we want human genome only.... Then why we are fusing it with mouse genome??? Please explain

[@gokulkrishna7411]

Thank you

[@roseb2105]

how to they identify the genes at electrophoresis to they put a tag ?

[@user-rq2cp6np4k]

it's really interesting!!! Thanks a lot!

[@syedarazvi4185]

Sir, somatic cell hybridization and somatic cell genetics-an alternative approach to gene mapping are thes both same? Please let me know at the earliest..it will be a great help for me.. Thanks and regards

[@vishvaseducation8011]

Will u pls tell me are these both same ?

[@deachanlhadol]

What about synkaryon??

[@allcontentinonechannel6970]

❤️❤️❤️

[@shomusbiologyofficial]

Thank you

[@ashar4331]

What is cell line?

[@MSTStrainrungaming]

sar plass hindi use in lacture

[@shomusbiologyofficial]

Please ka spelling thik karo. Not all individuals in India understand hindi

[@MSTStrainrungaming]

@@shomusbiologyofficial ok sar i agree

[@shubhamshrivastava289]

Sir topics Hindi me nhi h kya Apke pas

[@diya3095]

Cant understand

[@Ninojulz]

Lets find out how to create the next generation of mutants from somatic hybridisation human-mouse XD PLANET of 'HUM-RATS'

[@asmasaleem6441]

Boht complicated hy😥

[@asmasaleem6441]

Ye topic

[@ankitcreations9678]

Sir Your age before 8years ago🙂

[@shomusbiologyofficial]

I was in my master's

[@ankitcreations9678]

@@shomusbiologyofficial Sir Your are so talented❤️ ( sir mera b sapna hai zoology me master Banu par mera knowledge itna acha nahi hai😞😞) sir ab mera +3 3rd year( 5th sem.) chal raha hai. Sir mujhe ashirbad de taki Mera bhi MSc. Entrance Clear ho aur ek acha University me addmission kar paun.

[@Dagreton]

3:23 "Having a fused nucleus..." 2:49 "Nucleus never fused with each other" What is happening here ? Do you have any idea of what you say ? Had to dislike the video

[@sabujdutta-9561]

may be in case of human and mouse this fusion of nucleus is not possible but once we dissolve the walls with PEG we have three alternatives.... 1.their nucleus fuse along with cytoplasm(Synkaryon) 2.only cytoplasms fuse but not the nucleus(both the nucleus remain in the cytoplasm)(heterokaryon) 3.the cytoplasms fuse but during fusion one of the nucleus dies.(Cybrid) May be sir had to specify"never fuse" only because of the case of mouse and humans.