Generating Standard Curve and Determining Concentration of Unknown Sample in Excel

[@TeachingJunction]

How to Add Error Bars of Standard Deviation in Excel Graphs: kzbin.info/www/bejne/lXndf3uomKigrJY

[@VishakaMandal-f5f]

you saved my lifee, ThHANKYOUUUUU

[@TeachingJunction]

My pleasure

[@aishakhalfan2760]

where did you get that 0.484, answer please

[@nausheenghaffar3941]

can we use this equation to calculate conc. if unknown sample absorbance value higher than ploted values?

[@TeachingJunction]

Yes

[@kavyap4453]

Very useful.... thank you so much

[@TeachingJunction]

Welcome 😊

[@nailabashir-g1b]

Can you make a video on how to compare our results with a standard?

[@TeachingJunction]

Thanks for your suggestion. I have note down your suggestion

[@uzmahismat5829]

Thank u so much

[@TeachingJunction]

You are welcome

[@asifmohammed5717]

How did you find the value of concentration, as well as absorbance? please let me know Thank you

[@TeachingJunction]

You can get these values from experiment. I have used imaginary values for demonstration purpose

[@48_aufa41]

how do we know that the calibrated graphic that we made is correct sir?

[@TeachingJunction]

There are multiple factors. Firstly, make sure that your values falls into a linear range. Secondly, the value of correlation coefficient and coefficient of determination is also indicate the accuracy of calibration curve

[@NashwaAlg]

Please how to found the number 0.0249

[@nurainshamilabintikamalari7452]

Why u use 0.0249x like where did u get the value?

[@TeachingJunction]

That is the intercept value. you can get this value from regression equation generated by excel

[@sumithat07]

sir how did you get the value 0 .0249

[@NashwaAlg]

What is the answer please if you know now

[@qussaialiraqi3661]

why you chose value 0.484

[@thebluebeyond2329]

Please find the unknown concentration without the trend line equation. It's highly advicable not use the equation in most experimental reports

[@rmrmsafarii9542]

Do you mind sharing how to go about doing that?

[@akshaykirthan9281]

TREND(select the concentration of std.,select the O.D of std.,select the O.D of unknown sample) Do this in excel

[@oursoils2405]

you thumbnails videos hidden the video at the end of the video please remove it

[@TeachingJunction]

Thanks for your suggestion. I have modified the end screen

[@Urarto]

Don't use English title for your video if you are not making an english video. It is very cheep when you do a video in your own language and use a title from another languages.

[@TeachingJunction]

Actually, you are a cheap person. Think before you speak. I am using English language as teaching medium. The people from more than 50 countries are taking advantage from these video. You have a sick mentality