Genetic Engineering

How to isolate and copy a gene.
License: Creative Commons BY-NC-SA
More information at k12videos.mit.e...

[@celciajenifer4643]

And this is how a subject could be taught ...Kudos to your great effort 🎉... Totally loving this❤

[@hepburn118]

Absolutely brilliant!! I understand the process far better now, you should do more videos on DNA replication processes and cellular respiration etc. You have a natural gift teaching with sound scientific skill behind your methods.

[@trijetz3562]

I like how 99% of the comments are just about the woman and not the actual thing she is teaching

[@newbarrk]

I got recomened this video from a friend of mine, and it really helped me understand this for my biology a level, thanks!

[@srishtikumar5544]

This was AMAZING! Your explanations was crystal clear (and really creative) and it was really neat seeing you do the same thing in the lab. Looking forward to more!

[@mikefixac]

The really cool thing about being smart is it's totally appropriate to be goofy. Great instruction, thank you.

[@Ambri_DoesArt]

This was the easiest example ever! As a visual learner, it's way easier to understand than videos that don't further explain. Thanks!

[@lolaS2eric]

Nice video!! Great idea to explain with the draws! Just for the record... sometimes the background music was louder than your voice, so it got hard to listen and focus at these points. Thank you!

[@basherbasher2493]

ابنها ائ لتنلغابعفل

[@chumarine1115]

Plz, try to understand it, don't make a lame excuse!

[@brstudio7768]

This was a brilliant introduction to biotechnology, beyond impressive.

[@Corr7Blazers]

I'm watching this in 2016 and am watching this to study for my big exam tomorrow, helped a lot, thanks

[@matheuus05]

watching this video from Brazil and im so happy to learn with people who brings teachings from MIT

[@_biomacromolecules7782]

what a beautiful video! humour is definitely the way to go when the subject appears to be dreary. thanks for the effort guys!

[@Isha-tb4es]

you are AMAZING!!! Really did the perfect job of explaining what I find, a very complicated process with such clear illustrations and explanations. I'm sitting for my pure biology national exam paper tomorrow and this video is a god-sent blessing. Great job :-)

[@AqilDaiyan]

Everyone in the comments section have a crush on this woman Including me.

[@vika3192]

AlphieStudios boi

[@custodianvrael6471]

SIMP

[@dapet2258]

not me...

[@alekaditez3830]

no

[@trijetz3562]

bruh

[@akashalihazarika4690]

I have a doubt. Why not seperate the DNA pieces using gel electrophoresis at the very first place?

[@dergaming1751]

Neet ulal ne nai?

[@akashalihazarika4690]

@@dergaming1751 Do I know you?

[@dergaming1751]

@@akashalihazarika4690 apuni axomiya buli xudhilu

[@h.h.kumaraswamy4337]

An incredibly innovative way of teaching recombinant DNA technology..!

[@emmuita]

Watching this 2020 with online class. Very informative. Thank you

[@charlesmcdowell9436]

Crap i kept watching to vid because of how pretty she is then ended up becoming educated on how to isolate and copy a gene. It was done so well that even an idiot like me was able to understand it.

[@sandflea3789]

eew.

[@dubaigames929]

@@sandflea3789 what are you a kid

[@custodianvrael6471]

SIMP

[@snotsnarler1174]

@@custodianvrael6471, He may be a Simp but, he's the only simp which I will ever respect

[@aryans9334]

down bad

[@rowenmccormick9990]

Thank you so much! I am currently taking Biology 30 and the information that they were giving me in my lessons about genetically engineering recombinant DNA just wasn't making sense. You explained this concept so well, thank you!

[@nobodynohow8039]

I love it! It would have been helpful to show how you can run the restricted frog DNA and choose the appropriately-sized fragment for insertion into the plasmid, too.

[@ruggedbypowell]

nothing prepared me for the end!😂😂 thanks so much! 🎉

[@leanedias4594]

wow u just summarized a whole chapter (that was super hard before this video)! thanks for helping me pass!

[@jackwilkins1874]

Thanks so much. I have a science test tomorrow so this really helped me

[@ebunoluwataiwo9975]

Thank you my GCSE science exam is today and I should be in school but I'm really revising and this is going to help so much

[@SotaSports]

Great way to explain genetic engineering! Thank you :D

[@chandravenkatraman9544]

Thank you, this helped me understand REALLY well. It also helped me to answer the toughest questions in my exam.

[@s.z8223]

haha loved the cut outs of the plasmid and dna, makes it super easy to visualise whats going on

[@itsjohanne8269]

Hi thanks for the help. It was fun. A grade 8 student here. My module brought me here.❤

[@SHYLZATIPALAN-c5i]

Thank you so much Ma'am. So helpful for us to understand the genetic engineering process clearly.

[@zeltrioxo]

Brilliant visual guide! Maybe some closer detail into the 'sticky ends' and what not, but yeah really great video (y) You have gained a subscriber!

[@pateldishank1345]

The way you explained Thing used to make us understand Alll helped You totally nailed it🤗☺️

[@DiamondRose]

I need to show this to my teacher! It’s perfect for are unit rn

[@ninakoch1799]

Background music too loud Otherwise AWESOME video! Thanks!:)

[@theunpatrioticcroat6284]

0:23 i died a little bit inside

[@dominika4178]

It was way more easier and funnier to understand, the whole method. THANKS!

[@prajwalgupta5467]

Thanks yo very much I made this in science exhibition and won the first prize... 💯💯

[@AbhijeetKumar-py2vu]

Thanks for uploading this video. The way you told everything was amazing. Really helpful.

[@SH-qr7tw]

you did a great job and effort into this video! I appreciate it

[@arushidubey2000]

Nicely and simply explained.... 👍🏻👍🏻👍🏻👍🏻 My advice .....Please try not to have too much background noise/ sound effects...🙏🏻🙏🏻

[@allaboutwaterr]

Wow so simple and clear mamif I am your student I am sure I will do awesome in my exam. (I am a bad student)

[@dipteeshukla7]

she made this topic so simple im sobbing

[@drkomalpaul1864]

Awesome and creative video thanks a lot mam

[@firrialeksa3799]

Thank you so much, i really confused about genetic engineering chapter. Wish me luck for university test!

[@nashrahzaidi7878]

This is really awesome.... keep posting

[@leibling9111]

Great and simple explanation. Really helpful

[@user-pv1ng2od2n]

The last step could have just been putting the bacteria into a fluid containing ampicillin. The ones that didn't take up the plasmids (containing the ampicillin resistance gene) would've simply been destroyed.

[@shreyakrishna4403]

You are very creative! Loved the video and got to learn too :) Keep up the great work

[@andycrane551]

nice simple vid but it didn't go in to Gel electrophoresis... builds up to no conclusion :(

[@almostatheist]

Okay I'm a bio major but what you did seems a bit counter intuitive (please correct me if I'm wrong) You cut both frog Dna and Bacterial Plasmids Mixed them up and reassembled them with the help of Ligase then you INSERT the combined plasmids INTO bacteria, ONLY to EXTRACT them again for further analysis. Why would you insert the plasmids in the first place if you're just going to take them out for further analysis?

[@DeLaPenaFamily]

almost atheist They are reinserted back into the bacteria so that the bacteria can reproduce exact copies of the new bacteria, all containing the Frog DNA. Thus creating a Frog DNA "Factory". From a few modified bacteria cells you get many.

[@slotsize]

The initial insert of the frog DNA into the E. coli was to use the bacteria's replication machinery to mass produce the DNA since bacteria grows a lot faster. But as she mentioned, when the frog's DNA fragment is taken up by the plasmid you don't know which fragment is inserted. So to see if the desired DNA was inserted she take the [grown] E. coli and cut their DNA to be loaded on the gel along with the plasmids. The gel electrophoresis will separate the DNA depending on their fragments. Since the desired DNA fragment is larger than the undesired, you can visibly distinguish the fragments after running the electrophoresis. They can then just cut out the gel containing that band and use it to do what they want with it. At least, this is what I got from her video & from my lab lectures.

[@SalmansAcademy]

1 will multiply with bacteria into many... Multiple copies of edited Plasmid

[@xiaowenxu5720]

Your video is really interesting and explains things well. But l have a question that why don't first use gel electrophoresis to identify the frog gene instead of inserting all the gene into the bacteria and then cut it off and then identify? It seems more intuitive that we first find the right gene and then insert it into bacteria. And it may save bacteria😄. I am a year1 undergraduate student and can someone help me? Thank you very much!

[@CarolineZhang1]

Aren't the plasmids ampicillin resistant other than tetracycline resistant? Also, how are different plasmids distinguished from each other? What does the gel electrophoresis do?

[@WinxtotheWorld]

This video really helped me. Thanks!

[@justerfieldrapsang5240]

Jael Jordan

[@itsevcha]

Amazing explanation, helped me connect some dots

[@jaymarkechavez9311]

where can i get the picture of the final product? pls reply asap

[@anaali5mdu]

Appealing presentation...Good...

[@nias5114]

Thank you! This was very helpful!!!

[@saisohamdani7487]

Such a nice explaination that I've seen till today

[@juveriyahashmi4897]

For What course did you watch this video?

[@saisohamdani7487]

@@juveriyahashmi4897 for Biotechnology

[@melindatisado9842]

The gene of interest was transferred to another organism. Was the recipient of the gene of the same species as the original source?

[@neharahman7380]

what happens if we add tetracycline?

[@videsh7470]

I don't understand, what is the point of putting the plasmids into bacteria just to take them out again?

[@botabrat1]

Love this! I plan to use this to help My biology students!

[@elozJ1102]

Excellent illustration of Recombinant DNA

[@candy4lifeeeeee]

Best science video ever..., u are hilarious!!!

[@dvalle1100]

This video is adorable...so cute...I love the construction paper models!!!

[@tfoprincess]

You're awesome. I wanna do what you do XD fit in video making with my job.

[@jacksparrowismydaddy]

or I might be thinking "I clicked on this video about genetic engineering to learn something."

[@blue91r09]

thank you so much!! Absolutely an amazing video :D

[@mariafernandamoralesaleman8038]

Thank you so much for this video. I use it every year with my Biology students! (Leave a like if your my student and you are seeing this!)

[@GenZBro]

U have a very long name. Haha

[@Mrs.Kim2730]

Thank u sister u made it easy to understand💙

[@dararithvika9348]

thank you so much this helped me to uderstand easily more then lot of books ! can pls add the next steps too in detail

[@ShanniTheGreat]

So this is what my teacher spent two hours TRYING to teach me today???? lmao

[@pragatikhanorkar3480]

Superb thanks dear...it's became easy to understand for me now.

[@fassihbehramkhan5411]

You deserve appreciation 👍

[@MITK12Videos]

Folks, Elizabeth from this video just made a new one on the science of snot! Go check it out! kzbin.info/www/bejne/sHXSXoh9aM5qa9E&feature=autoshare

[@sriramprabakaran1907]

Mam finally u made it, which had not been happen's to me by any lecture 😂

[@MsAmJay]

Aw this is ingenious. And she's so cute! :)

[@natewilson431]

The background music was a bit too loud at times but the info was spot on.

[@TimerUchiha]

Really interesting, and you've made me understand this, so I thank you sincerely :D

[@PhantomXero]

Dude... Damn she's cute lol

[@lukivi2388]

i agree

[@custodianvrael6471]

SIMP

[@rimm_7797]

I understaaaand evvveeerrything. Your video is very helpful :) Thanks!!!

[@dv1150]

Coolest ma'am😂❤️😎 Tysm ma'am❤️

[@jsronarayne]

Thank you for this video! A big help! 🥰

[@latanezimbardo7129]

i assume u use pcr to replicate those DNA fragments?

[@OmKumar-wm4kb]

It's really fun..😊📚

[@JN003]

would it be possible to change the DNA of a bacteria that currently (i assume) consumes & digests (?) certain types of cells. Could the bacteria's DNA be changed to consume & digest cancer cells? Alternatively, would it be possible to destroy a cancer cell with a wave in the same way a glass is broken up when an opera singer reaches a particular note ? Are cells like different shaped glasses (ie different frequencies effect different cells in different ways ? ). thx !

[@theRANDOMNESSMASTER]

No because cancerous cells are basically body cells that divide uncontrollably due to mutated proto-oncogenes and tumour suppressors. Making a virus which target the body cell would kill the person that why it is so difficult to find a cure for cancer. I don't really understand what you are saying about the waves.

[@dusk4819]

This is an amazing video!!

[@jasminemandal7596]

thank you ... for making me understand dis...

[@hanakemeha9118]

awesome explanation guys thank you : )

[@samianholt]

Hmm if you cut the same purple piece out of the plasmid, doesn't that mean that it was pointless to put them into the in the bacteria first place? It glosses over how the gell box works, as I would of liked to see how that worked :) Other than it's a great explanation of the other steps.

[@dongakhiangte9932]

I like that💥, explanation material is amazing 💯

[@dipteeshukla7]

the end was so epic dayumnn🤣

[@vanishajalandhra2344]

Great help buddy thanks!!!

[@jedlong547]

whats the process at the end called, the electro one

[@BlondeNerdSam]

Gel electrophoresis :)

[@sameervyas8375]

Polyacrylamide gel electrophoresis

[@manisha8561]

I understood the concept soooooooooooooooooooooooooooo................................ well

[@mahamudakhanam3304]

Best video That was fun and easy

[@thekohhh]

where can i learn about genetic engineering? which degree and major do i need to take? i wish to be a genetic engineer...

[@thekohhh]

is biotechnology the same as bioengineering?

[@thekohhh]

university in my country only offer degree&major in biotech.. none of them offer bioengineering.. and so i enrolled for a degree in biotech. should i major in biotech? or should i find a university oversea that has bioengineering? are both the same thing?

[@imbatman8472]

it really is amazing what they can do.

[@wolfania]

very well explained!

[@Dhruvbala]

This was amazing! Thanks!

[@adilmonteiro3337]

We can produce testosterone by this method ?