Thanks for this presentation. The explanation was very clear. I'd like to read more about the subsystems to annotate genomes, could you provide any link to a paper in order to know more about the subsystem approach? (I'm reading now the 2005 Nuc. Ac. Research about the 10 thousand genomes, but any other reference would be appreciated). Also, could it be possible to annotate genes with the subsystem methodology using high throughput transcriptomic data from an eukaryote with no sequenced genome?