How do you use a Micropipette? A step-by-step guide!

The Micropipette is the most important tool in the molecular biology lab - but how do you use one?
0:00 Introduction
0:19 What is a micropipette?
0:52 Step 1: Choose the right micropipette
1:46 Step 2: Set the volume
2:50 Step 3: Use a fresh tip
4:09 Step 4: Draw up and dispense the liquid
5:35 Step 5: Eject the tip
This practical guide introduces you to the basics of how to use a Micropipette with advice for things to avoid and Pro-Tips so you can hit the ground running.
Cartoon Illustrations by Michelle Lotker
This video is copyright Jayme Dyer, published under the Creative Commons CC-BY SA 4.0 license.
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[@angiem2192]

You’re amazing. 10 years in biology. And not one professor has ever said THE SECOND STOP IS FOR DISPENSE ONLY. this fixed every experiment I have for micro this semester thank you

[@YouTooBio]

Yay I'm glad my video was helpful!

[@bnrakesh9156]

​​​@@YouTooBioYes ma'am your teaching is super🙏🙏🙏 You are enhancing both the theoretical n practical knowledge about the topic. Thank you so much ma'am

[@prophetlocs7040]

You’re video has been the best in explaining everything in detail yet very easy to understand, thank you.

[@mubrra4748]

The attention to detail is fantastic here .....every possible point is covered with the utmost clarity 😮

[@jamiehollanders3259]

Clear, concise and fun video! Perfect for students and beginners like myself. Thank you!

[@YouTooBio]

Glad it was helpful!

[@OffGridInvestor]

FAR better than the first video I watched. It couldn't explain the 2 stops properly. Another one of those videos where the person explaining it is like he's explaining it to someone who already knows.

[@dannachavez275]

this has been so helpful. thank you so much. I won't be walking in clueless into my intro bio lab now.

[@YouTooBio]

Glad it was helpful!

[@osmirod1]

I love teachers like you. Thank you 😊

[@YouTooBio]

Thank you! 😃

[@DrFodz]

That was a very helpful, informative, fun, and memorable video! Clearly a lot of thought and work has been put into it and it SHOWS Love your style!

[@YouTooBio]

Thanks so much - glad it was helpful!

[@imperpekto12ify]

I like how you made this video so friendly and funny at the same time. Thank you for making the video.

[@YouTooBio]

Yay glad you found it to be approachable!

[@SaraGreen-xr3tl]

What a fantastic and concise explanation. Thank you!

[@anime991]

This is very helpful. I have to teach some new undergraduate students how to use micropipets but I was having a hard time figuring our how to accurately convey what I know in a way that makes sense to people with no lab experience

[@fishfish20]

I'm liking your channel. I'll be working on a lab soon, so I'm familiarizing myself before the training. 😂

[@bluesteno64]

Just had the pleasure of watching this in my AP bio class. I laughed quite a few times! Thank you for putting this video together

[@YouTooBio]

Glad you liked it!

[@queenesther.chukwuka]

You’re an amazing Teacher ma’am. I love the demonstrations ❤

[@madi6880]

Tomorrow is my first in-person second-year lab, so I was looking to refresh my memory on how to use micropipettes, and this video was perfect! It was so easy to understand and all of the visuals and animations helped so so much. I was worried about not remembering how to use the micropipettes, as I never got to physically use them in my first-year labs due to everything being online because of COVID, but now I can say I'm fairly confident, and even excited to use them, all because of this video :)

[@YouTooBio]

Hooray! Glad the video was helpful. Good luck in lab!

[@madi6880]

@@YouTooBio Thank you!

[@himilyiasriprabagaran2691]

Loved the way you explained it in detail way simpler. Thank you!!

[@naomia9848]

thank you so much! I have a micropipette practicum today, this was super helpful!

[@Ceecee_00000]

Very Informative! Thank you

[@crochetbyhafsaaa]

this video legit saved me before my OSPE exams, thankyou🥰

[@1313babinski]

The best I’ve seen you covered pretty much all possible question great great video!!!!!

[@umba9610]

Thank you for this video. It is so helpful. I have to learn this immediately

[@bulbulshaikat4417]

Very well said. Thanks from Bangladesh 🇧🇩

[@jamesdeancafino1240]

I really liked the way you explained micropipettes. Thank you.

[@YouTooBio]

Glad you enjoyed it!

[@alitahir7471]

I watched too many videos but this one is perfect like the best thank u ma'am ❤️

[@BChew-ku9he]

Thank you for your video!!! You helped me with my lab homework!! ❤️❤️

[@YouTooBio]

Hooray! Glad it was helpful

[@Sp-fe1eo]

thanks for the video...best explaining...easy to understand and helped me a lot...love this channel ❤

[@YouTooBio]

Thanks!

[@primalshasandunigamage5682]

Wow..ur explanation is clear and amazing 😘😘

[@christinephilip3849]

Really helped me in doing my experiments properly 👍🏻👍🏻

[@sydneykate]

Thank you for this video!

[@faheenan385]

Thank you so much, u made my day 😊😊

[@snarlymacsnarls488]

This is such an amazing tool, thank you for such a thorough and entertaining video 💪🏻😄

[@YouTooBio]

Thanks! ❤️

[@o.m9514]

Cool! Thank you, your video makes learning about an equipment, easy, breasy and fun!

[@YouTooBio]

Thanks!

[@DarkSoul-du1ck]

The video is very useful and understandable. Thank you very much !

[@YouTooBio]

Thanks! Glad it's helpful.

[@debjitbanerjee8512]

Please make more such amazing videos...

[@nalin_gunathilaka4067]

excellent explanation

[@cookwithme1698]

Very helpful video thanks

[@chinememila6998]

You are so awesome...you made this fun and I understood everything thank you 😊

[@YouTooBio]

Yay! Glad it was fun

[@lbe1932]

Thank you very appreciated :)

[@Sabridemir1981]

great video!!!

[@khairatallyamour5117]

Understand...enjoyable

[@mohammedidreesshafi4244]

Thank u

[@user-nu4gn3nx8m]

thank you so much...

[@lorazam_]

The best video I have ever seen

[@nourhanelsherif2787]

Thank you 🌷🌷

[@aslhanv6531]

such an amazing video that really teach me cool tips

[@YouTooBio]

Glad you liked it!!

[@arianmahi1915]

So helpful for me☺ really cool

[@Alchemist_171]

Thank you so much!!

[@YouTooBio]

You're welcome!

[@vyankateshzambare1440]

I liked how you added those dramatic Eppendorf tubes reactions in the video🤣

[@fizarafique8150]

So nice of you mam amazing lecture 😊

[@YouTooBio]

Thanks a lot 😊

[@habibullah1090_]

Awesome

[@shrishtinarang8650]

Very nice video You are soo cool. Wish could take you to my lab . Need this kind of cool

[@Felix-dt1tu]

Amazing video, really liked everything, especially the tips and the warning being so clear, BUT please mention somewhere that at 2:18 you made a mistake on the biggest pipette saying "0 6 5" equals 650qm, please make a note in the top comment. For the rest; amazing Thanks a lot

[@gheedalfawaz370]

You are an angel

[@kiarajelo87]

love this! do u have a videoo saying the right way (angle) to pipette?

[@YouTooBio]

I don't! What is your vision for that video?

[@beyuorichard968]

Good

[@tchistermorrelebissa8628]

Good demonstration, Madam, but could we know which graduation is considered for a given micropipette? I mean, for example in one pipette there are three (03) numbers appear and which number to consider?

[@Ammu______Nisha]

You are very nice 🙂🙂🙂🙂❤️❤️❤️❤️❤️❤️

[@keyspark]

after watching this video, I feel like a mircopipetting pro

[@pandjnixon]

Great teaching video ! - You no doubt can recycle the used tips by autoclaving ?

[@YouTooBio]

In theory, yes but most people don't. There's no automated way to clean and sort them back into tip boxes, so it's practically very expensive (labor) to clean, autoclave and sort.

[@pandjnixon]

@@YouTooBio OK - Thanks Jayme.

[@osmirod1]

I love you.

[@michaeldurairajs6696]

Does the depth at which a tip is sent into the "sample" effect the accuracy as small portion is stuck outside especially for the 20 ul pipettes?

[@Ahmed-zu9yv]

Yes.the Depth is very important. Its proportional directly to the volume you have to draw.

[@Mark-zo4zh]

I have a bunch of these how do I sell them

[@AleForFun]

How without a stand for both micropipette and tips?

[@tasneemabdulsattar7417]

Isn't 065 65ul and not 650 ul?

[@polo8601]

Ma'am let's say we need to pipette out 1500µl...and we have a P1000 micropipette.....then first we have to set 1 0 0 to pipette out 1000µl and then AGAIN we have set the volume display as 0 5 0 to pipette out 500µl more....total 1000+500=1500µl....is that the correct procedure mam ?

[@YouTooBio]

You can do it that way, but it would be faster to do 750ul twice.

[@IdiotWithEducation]

If I were to use this to extract rotifers from a slide, what sized tip would I need?

[@YouTooBio]

I would try a p1000 tip. If the opening of the tip is too small, cut a little piece of the tip off with a razor blade to make the opening a little bigger.

[@IdiotWithEducation]

@@YouTooBio thanks so much!

[@jcdyer3]

Two viewers were looking for macropipettes.

[@juliusmaime5959]

careful pi petting ,check tutorial for fixed pi petting .