How to Calculate CFU per ml of Bacterial Sample? in 3 Steps || cfu/ml in Microbiology

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[@GraftingAvocados]

I'm a farmer not a trained scientist But I wanted to check my own cfu on the biological products I buy. This video is the best step y step. Thanks a lot for shearing your pedagogical skills

[@biologyexams4u]

Dear Friends Thank you so much for watching @biologyexams4u Please consider subscribing using the link: bit.ly/3kG2kKf A simplified video explaining to calculate cfu/ml of original stock? Hope this will help Watch, Understand and enjoy Biology :)

[@drnickatnyte2688]

This is the best crash course I could find. Thank you so much!

[@edwinkaroney5370]

It has never been made this easy before. Thank you.

[@MiorAkif]

I love how concise and to the point this explanation is. Helped me a bunch!

[@172na]

Thanks 🎉 you saved me an hour before my Microbiology test 😊 You are my G.O.A.T

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Thank you so much! I'm taking A-level Biology and you explained it so well :)

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thank you so much Sir. this is very helpful in doing the research i'm doing right now

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Thank you so much. I just couldn't figure this out with the materials provided to me by my professors. It's so hard sometimes to figure out these things out for yourself, when the given material is always in a foreign language and has many terms, which you only are familiar with half at the best circumstances..

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Thank you sir 🤭 now Im ready for my exam!!! 😂🤗

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[@aleaoleandra7116]

Thank you so much sir, it's so helpfullllll

[@muhammadkaleem4251]

Nicely explained. Thank you

[@adriannarodriguez8805]

Thank you very much greatly helpful

[@biologyexams4u]

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This is a nice video. well explained. Thank you sir for the video.

[@rajadurai283]

Good sir.easily understand.thank you sir

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All the best

[@rezvanmohammadi9834]

Thanks a lot 🙏

[@biologyexams4u]

Welcome Rezvan...Thank you:)

[@P_poo]

Thank you so much 😭🫶

[@angelicadin08]

thanks. it helps a lot

[@bobi9281]

Nice explanation

[@MarykMakwelele]

Life saver ❤

[@bilill24]

Thank you so much ❤!

[@SinhaSahab1]

Very well explained

[@Matt-or9tq]

very helpful thanks

[@avasava7720]

alternatively, couldn't you use: no. of colonies/ TDF x Volume of culture in mL? As long as the units are consistent between DF and Volume and multiply those two values and then divide that by the colony value

[@gustinfinnegan9000]

Very helpful, thank you for making this video, i have a question, sir what if we have 2 plate for each dilution (duplo) how to calculate ? Example: 10^-3 10^-4 Petri 1 287 31 Petri 2 292 42 Vol of stock transferred = 0.1 ml Thank you, sir

[@emekaokey511]

Take the average

[@drsirgay9176]

thanks man, great video!

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Thank you for your nice words Please consider subscribing and sharing with your friends Thank you so much:)

[@cartoonanimationlovers-8922]

Thank you so much you explained it very well 1st time I tolerate it ^_^ Please I have a Q, might be silly. now after counting it plate, tube (f) looks has low count, but after calculation it will be 10*8 in compared to tube (e). Does it mean higher count (f)? .

[@biologyexams4u]

Welcome.. Thank you for the question. We often wont consider the plate with low count or high count as the chance of error is high. Hope I answered the question.

[@almacazares3405]

Thanks times 10 raised to 10000000

[@biologyexams4u]

Thank you so much. really appreciate your creativity. happy to know the video helped. Pinning this comment. take care, stay blessed

[@savitasoni719]

Hello sir Thank My question is Sample of E. Colo it is use one loopful cuture on the plate

[@rajeshp6444]

Hello, could pls help me, I ,0.5ul of stock solution diluted in 495.5ul of diluent what is the dilution factor

[@hanimaisaraa]

Do i have to count for the overall average for the three plates or i shouldve just ignore the ones tgat are not in range?

[@sajithkarunaratne8965]

How do you get ten into the plus numbers? The calculations gives 10 to the (-) values Right?

[@biologyexams4u]

Hi Sajith We are using dilution factor not dilution.. Hope this video will help kzbin.info/www/bejne/e4DWioKcgLKaiZo Thank you so much

[@sajithkarunaratne8965]

@@biologyexams4u OK. Thank you so much for understanding me. Got it.

[@AdnanAfzaal22]

Great presentation. Why we took 0.1 ml and how we can take such minute quantity.

[@biologyexams4u]

Small quantity; there wont be overcrowding of bacterial cell. We can take small amount using micropipettes. thank you

[@dieudonnenyamaifofe9646]

You can use calibrate your micropipette at 10 microliters (=0.1 ml)

[@debabanighosh4950]

Your videos are excellent, if possible please make some videos on PCR, Elisa, buffer preparation, sds-page, electrophoresis, endotoxin test and their calculations.

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[@arunahsubramaniam7006]

hi, If we use raw samples there will be have dilution factor. Also how to count for raw samples transfer into plate 0.1ml.

[@tanishakumari2958]

First E. Coli sample O.D is what we consider?

[@a.s9509]

What if I want to have a bacterial solution with 1*10^7 for example. How do I have to prepare it with this 6.3*10^7 solution?

[@sabinakhatri7734]

if you diluted 1ml of a sample with 99ml of sterile water then plated 100ml of the diluted sample on plate count agar and 75 colonies were found. calculate the cfu/ml of the original undiluted sample can you please help with this

[@syamilahshaarani]

From the 6.3 x 10^7, how to make it 10^6 bacterial suspension?

[@ammardilawar510]

What is the formula if we get countable colonies in two plates ?

[@fazalmaqsood907]

Sir how to calculate aliquot factor in endophytic analysis??

[@berlindaantwi605]

What if all of them are within range, will you find the CFU/ml of each and then find the average?

[@padpakbboong]

Thank you

[@Qson2321]

how can we get 10^7 as final when we had 10^6 after 10^5/0.1?

[@AdnanAfzaal22]

Great

[@Dineesh86]

Why is vol. of culture plated 0.1 ml and not 1 ml as you use 1 ml from the total 10 ml?

[@biologyexams4u]

If 1 ml is used there is always a chance of overcrowded plates

[@dieudonnenyamaifofe9646]

@@biologyexams4u then the 63.10^6 CFU should be in 0.1 mL not 1 mL and afterward express it in CFU/ mL

[@rajakumarangb2912]

how is the voulme plated i.e 0.1 ml is calculated?

[@rekhapandey5363]

i have a broth culture of a bacteria and i need 10^9 CFU/ml concentration of it to inoculate in a material. Can you help me how i can do that?

[@biologyexams4u]

What is your colony count? Did you do serial dilution and plated on an agar plate?

[@mandeepnain6162]

Dilution will be done by adding media or distill water or buffer

[@biologyexams4u]

Hi Mandeep Phosphate buffered solution or isotonic saline solution (0.85%)is commonly used as both wont causes osmotic shock..other diluents is Sterile distilled water . Often the selection of diluents depends on the further procedure and also the type of microorganisms ... Like for cell count or for subculturing.. Thank you

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[@hananesaidani7059]

c'est quoi la différence entre ufc/ml et ufc/g ? quand on doit calculer par ml et quand on doit calculer par gramme ?

[@souryajyotidash2096]

Hello Sir, I've a question. How to prepare 4×10⁸cfu/ml ? An earliest response will be immensely valuable.

[@MohammedMusa-t7s]

How to get volume of culture plate

[@keepgoing6630]

How do we know the number of colonies?

[@biologyexams4u]

Using bacterial colony counter... Thank you

[@sharisalee6347]

not understanding how it went from 10 to the 6th power to 10 to the 7th power though can someone explain?

[@biologyexams4u]

Hi Sharisa Lee Which part? Is it the final calculation? 63X10*6 and 6.3x10*7 both values are same.

[@sharisalee6347]

yes, you were at 10 *6 but final answer became 10*7 so I'm not sure how final answer became 10*7

[@sharisalee6347]

is it because once you move the decimal over to the left to get 6.3 instead of 63 , the 10*6 changes to 10*7 ?

[@mahendrathalari9878]

Nice

[@mahamfatima6093]

Didn't get the 0.1ml part. Could anyone explain?

[@UnKnown-sp5ye]

How did you get 443, 63, and 3 colonies?

[@meditationrelaxingpeaceful5632]

Someone give the value of total number of cells 83x 10 power 8. Is it possible.. please clear calculation if possible

[@hananesaidani7059]

what is the difference between ufc/ml and ufc/g ?

[@ahlemahle7491]

ML for when we use a liquid sample (eg milk) G for when we use a solid sample (eg a sandwich)

[@CoffeyDeirdre]

1590 Annie Plain

[@akgarg100]

Total dilution factor ..? How to calculate that ?

[@biologyexams4u]

Hi hope this will be helpful in understanding dilution factor kzbin.info/www/bejne/e4DWioKcgLKaiZo

[@sauravsagar8735]

Serial dilution se

[@saifurrashid8913]

Volume of culture plated should be 1 ml

[@biologyexams4u]

This is the standard procedure as per the manual. There may be change in volume.. Yes... Thank you

[@rimadessai6625]

Sir why we don't consider minus sign of dilution when doing calculations ?

[@biologyexams4u]

Really appreciate your question.. Here we are using dilution factor not dilution.. Hope this video on dilution factor will help kzbin.info/www/bejne/e4DWioKcgLKaiZo Thank you so much 👍

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