How to calculate concentration of TBE or Tris/Borate/EDTA buffer

[@CALAMKaK]

Which of the following amino acids would you LEAST expect to find in the trans- membrane region of a G Protein-Coupled Receptor? A. Alanine B. Leucine C. Proline D. Tryptophan E. Valine

[@GeneticsLessons]

Marat, they are all non polar and can be found in non polar part of the membrane, but probably we least expect to find Proline cause it is most ridged amino acids - and usually polypeptide chain has abrupt turn where is a proline - which is unlikely for transmembrane part of a protein.

[@CALAMKaK]

Thanks