How To Calculate The Isoelectric Point of Amino Acids and Zwitterions

[@TheOrganicChemistryTutor]

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How did you determine which charged group gets deprotonated first?

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[@acecold6316]

i was literally having a breakdown over how complicated my teacher made this, youre a lifesaver dude

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[@jianeecarrasco9106]

literally me

[@lh9591]

Thank you so much! My biochem professor just told us "use the pKa values that are closest to each other, that is usually the answer."

[@bobbykang5332]

thats what my prof said too...

[@redpilledbachelor7776]

use only two pka, not three.

[@RirichiyoShirakiin]

but at 12:04 9.1 and 10.5 is closer than 2.2 and 9.1

[@sebastianelben4795]

Quick tip that helped me understand „zwitterions“ better: „zwitter“ is the german word for hermaphrodite, so a zwitterion, (or „hemaphrodite-ion“) is hemaphroditical by having both a positive and negative charge

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My Biochem exam is on monday. Thanks for uploading the video

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WOW so much easier to comprehend with the number line...understood it instantly

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[@henrydillon2115]

You just helped a dude who randomly selects two pka values to calculate for the pI and just hope for the best, whenever the pka values are 3..........and now you make him wanna speak spanish: gracias

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[@sreemahalakshmivlogs]

iam very fear about this topic when ever i saw your video of calculation of ISP of amino acids . now i got very good confidence in this concept. you explanation is awesome. thank you so much sir

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Thanks man You’re better than my lecturers

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If you have not put this video on KZbin, I would not have understood any content about PI from references. Thanks a lot!

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[@tisharani519]

Thank you sir , for helping me understand the application of the formula

[@bethelsamson7434]

great video! at 0:11 you say glycine has chiral carbon, but it's actually the only AA without a chiral carbon because the R group is just hydrogen

[@svda814]

He says _an achiral carbon_

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[@askmargo]

Thank you!! My professor didn't even try to teach this, he just read off of the slides 😢

[@ananditatiwari21]

Best video on isoelectric point

[@MayMoorefield]

Why do the pKa values for the amino group and carboxylic acid change from problem to problem?

[@ellieberri]

i also wonder why

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[@ariaman-]

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[@aprajitapaliwal9217]

Glycine does not have a chiral carbon ( it is the only amino acid without an asymmetric carbon)

[@stevennguyen7930]

totally agree

[@nimasabri4000]

He said Achiral meaning not Chiral...

[@Sankalp-sd6fm]

So

[@justdeiax]

Why do i understand this better than our biochem class???

[@BossAwesome11]

Glycine doesn’t have a chiral alpha carbon. In the beginning of the video you said chiral carbon but Glycine isn’t chiral. Thank you for all you do just wanted to clarify.

[@veronicachauke2031]

He said achiral

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[@MrDarth44]

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[@ozumeceozcan6231]

umm i thought glycine did not have a chiral carbon. am i wrong to assume that a chiral carbon is a carbon atom that has 4 different groups binded to it?

[@cheeragsridhar1696]

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[@blue_._.3_]

OMG YOU GOT RIGHT TO THE POINT THANK YOU

[@CyrusMuema-q8q]

Excuse me sir have a question,,,using glycine as an example will it also not exist as zwitterion at a Ph of 7 which is also in between the two pkas

[@Honestpath4]

Please make a video on Newton's 2nd law of motion (Physics) specially on problems like . " YOU ARE MOVING A DESK ACROSS A ROOM AND MUST USE A FORCE OF 467 N . IF THE MASS OF THE DESK IS 65.0KG AND ITS ACCELERATING AT 1.22m/s,WHAT IS THE COEFFICIENT OF FRICTION BETWEEN THE DESK AND THE FLOOR?

[@harshilpatel7855]

I think the answer is 0.733 Here is what i thought - You need atleast a force 467 N to move it which must be equal to the limiting force of friction. Also limiting force of friction = umg ( since mg=N by FBD and u is coefficient of friction) So 467=umg 467/(65*9.8) = u u= 0.733 If g= 10 then u=0.71

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[@beatricedawn9567]

I really enjoyed this video and understood it as well. But when calculating the PI I was taught that we take the pka values to the left and the right of the zwitterion. Please I really need a quick response😢

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very good explanation.