How To Calculate The Isoelectric Point of Amino Acids and Zwitterions

This biochemistry video tutorial explains how to calculate the isoelectric point of amino acids. It also explains how to identify the zwitterion structure of an amino acid.
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How did you determine which charged group gets deprotonated first?

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i'm sorry, professor? dude i learned this on high school wtf? is this college level?

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[@lh9591]

Thank you so much! My biochem professor just told us "use the pKa values that are closest to each other, that is usually the answer."

[@bobbykang5332]

thats what my prof said too...

[@redpilledbachelor7776]

use only two pka, not three.

[@RirichiyoShirakiin]

but at 12:04 9.1 and 10.5 is closer than 2.2 and 9.1

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[@sebastianelben4795]

Quick tip that helped me understand „zwitterions“ better: „zwitter“ is the german word for hermaphrodite, so a zwitterion, (or „hemaphrodite-ion“) is hemaphroditical by having both a positive and negative charge

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Bro this helped me so much I can't even tell you. My biochem professor did a VERY poor job explaining this

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iam very fear about this topic when ever i saw your video of calculation of ISP of amino acids . now i got very good confidence in this concept. you explanation is awesome. thank you so much sir

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You just helped a dude who randomly selects two pka values to calculate for the pI and just hope for the best, whenever the pka values are 3..........and now you make him wanna speak spanish: gracias

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This was such a good explanation! No more crying into my biochem textbook

[@bethelsamson7434]

great video! at 0:11 you say glycine has chiral carbon, but it's actually the only AA without a chiral carbon because the R group is just hydrogen

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He says _an achiral carbon_

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[@aprajitapaliwal9217]

Glycine does not have a chiral carbon ( it is the only amino acid without an asymmetric carbon)

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He said Achiral meaning not Chiral...

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umm i thought glycine did not have a chiral carbon. am i wrong to assume that a chiral carbon is a carbon atom that has 4 different groups binded to it?

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[@BossAwesome11]

Glycine doesn’t have a chiral alpha carbon. In the beginning of the video you said chiral carbon but Glycine isn’t chiral. Thank you for all you do just wanted to clarify.

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He said achiral

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Why do the pKa values for the amino group and carboxylic acid change from problem to problem?

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i also wonder why

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[@harshilpatel7855]

I think the answer is 0.733 Here is what i thought - You need atleast a force 467 N to move it which must be equal to the limiting force of friction. Also limiting force of friction = umg ( since mg=N by FBD and u is coefficient of friction) So 467=umg 467/(65*9.8) = u u= 0.733 If g= 10 then u=0.71

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